Immobilized Methods and Application of Immobilized Saccharomyces cerevisiae Cells

2012 ◽  
Vol 518-523 ◽  
pp. 218-222
Author(s):  
Ying Zhu ◽  
Jian Ping Zhou ◽  
Zhi Ye Wang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Immobilized Cells ◽  
Continuous Fermentation ◽  
Production Capacity ◽  
Optical Microscope ◽  
Superior Performance ◽  
Low Costs ◽  
I Cells ◽  
Electronic Microscope ◽  
Ca Alginate

The study was to find low costs and superior performance immobilized carrier for microorganism cells using in industry. Ca-alginate , Agar , Gelatin-glutaraldehyde and polyvinyl alcohol (PVA) were investigated to immobilize Saccharomyces cerevisiae cells, and immobilized cells’ production capacity for ethanol, contents of ethanol and mechanical strength were also studied. Then, optical microscope picture and scanning electronic microscope pictures were given to research its microenvironment. Finally, Batch and Continuous fermentation with PVA immbiolized Saccharomyces cerevisiae were experimented. The PVA immobilized method is the best in the four methods with concentration of 8%, microscopic observation also offered a proof that PVA material was fit for immobilizing cells with the traits of polycellular and reticulate. At last, the production capacity for ethanol and reactivity were superior to free cells in batch and continuous fermentation. This research could provide important information on the commercial utilization immobilized-cells for biotransformation.

scholarly journals Food Grade Ethanol Production Process of Sorghum Stem Juice Using Immobilized Cells Technique

2015 ◽  
Vol 9 (7) ◽  
pp. 8 ◽  
Author(s):  
Tri Widjaja ◽  
Ali Altway ◽  
Arief Widjaja ◽  
Umi Rofiqah ◽  
Rr Whiny Hardiyati Erlian
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Sieve ◽  
Zymomonas Mobilis ◽  
Immobilized Cells ◽  
Ethanol Yield ◽  
Continuous Fermentation ◽  
Continuous Process ◽  
Pichia Stipitis ◽  
Batch Process ◽  
Food Grade

One form of economic development efforts for waste utilization in rural communities is to utilize stem sorghum to produce food grade ethanol. Sorghum stem juice with 150 g/L of sugar concentration was fermented using conventional batch process and cell immobilization continuous process with K-carrageenan as a supporting matrix. The microorganism used was Mutated Zymomonas Mobilis to be compared with a mixture of Saccharomyces Cerevisiae and Pichia Stipitis, and a mixture of Mutated Zymomonas Mobilis and Pichia Stipitis. Ethanol in the broth, result of fermentation process, was separated in packed distillation column. Distilate of the column, still contain water and other impurities, was flown into molecular sieve for dehydration and activated carbon adsorption column to remove the other impurities to meet food grade ethanol specification. The packing used in distillation process was steel wool. For batch fermentation, the fermentation using a combination of Saccharomyces Cerevisiae and Pichia Stipitis produced the best ethanol with 12.07% of concentration, where the yield and the productivity were 63.49%, and 1.06 g/L.h, respectively. And for continuous fermentation, the best ethanol with 9.02% of concentration, where the yield and the productivity were 47.42% and 174.27 g/L.h, respectively, is obtained from fermentation using a combination of Saccharomyces Cerevisiae and Pichia Stipitis also. Fermentation using combination microorganism of Saccharomyces Cerevisiae and Pichia Stipitis produced higher concentration of ethanol, yield, and productivity than other microorganisms. Distillation, molecular sieve dehydration and adsorption process is quite successful in generating sufficient levels of ethanol with relatively low amount of impurities.

scholarly journals Mead Production Using Immobilized Cells of Saccharomyces cerevisiae: Reuse of Sodium Alginate Beads

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 724
Author(s):  
Miguel L. Sousa-Dias ◽  
Vanessa Branco Paula ◽  
Luís G. Dias ◽  
Letícia M. Estevinho
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sodium Alginate ◽  
Fermentation Process ◽  
Immobilized Cells ◽  
Alginate Beads ◽  
Production Medium ◽  
Alcohol Content ◽  
Volatile Acidity ◽  
The Matrix ◽  
Second Category

This work studied the production of mead using second category honey and the immobilized cells of Saccharomyces cerevisiae in sodium alginate, with concentrations of 2% and 4%, and their reuse in five successive fermentations. The immobilized cells with 4% alginate beads were mechanically more stable and able to allow a greater number of reuses, making the process more economical. The fermentation’s consumption of sugars with free cells (control) and immobilized cells showed a similar profile, being completed close to 72 h, while the first use of immobilized cells finished at 96 h. The immobilized cells did not significantly influence some oenological parameters, such as the yield of the consumed sugars/ethanol, the alcohol content, the pH and the total acidity. There was a slight increase in the volatile acidity and a decrease in the production of SO2. The alginate concentrations did not significantly influence either the parameters used to monitor the fermentation process or the characteristics of the mead. Mead fermentations with immobilized cells showed the release of cells into the wort due to the disintegration of the beads, indicating that the matrix used for the yeast’s immobilization should be optimized, considering the mead production medium.

Use of Immobilized Candida Cells on Xylitol Production from Sugarcane Bagasse

2000 ◽  
Vol 55 (3-4) ◽  
pp. 213-217 ◽  
Author(s):  
Walter de Carvalho ◽  
Silvio Silvério da Silva ◽  
Michele Vitolo ◽  
Ismael Maciel de Mancilha
Keyword(s):  
Sugarcane Bagasse ◽  
Immobilized Cells ◽  
Alginate Beads ◽  
Candida Guilliermondii ◽  
Volumetric Productivity ◽  
Xylitol Production ◽  
Experimental Conditions ◽  
Work Volume ◽  
Yield Factor ◽  
Ca Alginate

Abstract In this study we used the yeast Candida guilliermondii FTI 20037 immobilized by entrapment in Ca-alginate beads (2 .5 -3 mm diameter) for xylitol production from concentrated sugarcane bagasse hemicellulosic hydrolysate in a repeated batch system. The fermentation runs were carried out in 125- and 250-ml Erlenmeyer flasks placed in an orbital shaker at 30 °C and 200 rpm during 72 h, keeping constant the proportion between work volume and flask total volume. According to the results, cell viability was substantially high (98%) in all fermentative cycles. The values of parameters xylitol yield and volumetric productivity increased significantly with the reutilization of the immobilized biocatalysts. The highest values of xylitol final concentration (11.05 g/1), yield factor (0.47 gig) and volumetric productivity (0.22 g/lh) were obtained in 250-ml Erlenmeyer flasks containing 80 ml of medium plus 20 mi of immobilized biocatalysts. The support used in this study (Ca-alginate) presented stability in the experimental conditions used. The results show that the use of immobilized cells is a promising approach for increasing the xylitol production rates.

Semicontinuous and Continuous Production of Citric Acid with Immobilized Cells of Aspergillus niger

1987 ◽  
Vol 42 (4) ◽  
pp. 408-413 ◽  
Author(s):  
H. Eikmeier ◽  
H. J. Rehm
Keyword(s):  
Citric Acid ◽  
Aspergillus Niger ◽  
Immobilized Cells ◽  
Continuous Production ◽  
Continuous Process ◽  
Batch Experiments ◽  
Medium Exchange ◽  
Free Cells ◽  
Semicontinuous Cultivation ◽  
Ca Alginate

Abstract The citric acid excretion of Ca-alginate-immobilized cells of Aspergillus niger in batch culture decreased with a half-time of approximately 19 days. Reactivation of the biocatalysts by regeneration in growth medium was possible, but it was followed by a submerged sporulation of the fungus, and medium was highly contaminated with free cells. Citric acid production could better be prolonged by semicontinuous cultivation with medium exchange every 7 or 14 days, respectively. After 32 days the remaining activity in semicontinuous culture was 1.4-fold higher than in comparable batch experiments. Similar improvements were obtained with a continuous process at a dilution rate of 0.125 v/v · d, whereby medium efflux completely free of detaching mycelia.

High ethanol productivities using small Ca-alginate beads of immobilized cells of Zymomonas mobilis

1981 ◽  
Vol 3 (11) ◽  
pp. 613-618 ◽  
Author(s):  
Argyrios Margaritis ◽  
Pramod K. Bajpai ◽  
J. Blair Wallace
Keyword(s):  
Zymomonas Mobilis ◽  
Immobilized Cells ◽  
Alginate Beads ◽  
High Ethanol ◽  
Ca Alginate

Desulfurization of Model Oil Containing Dibenzothiophene or 4,6-Dimethyl Dibenzothiophene by Alginate Immobilized Cells

2011 ◽  
Vol 233-235 ◽  
pp. 1171-1176
Author(s):  
Ming Fang Luo ◽  
Hui Zhou Liu
Keyword(s):  
Immobilized Cells ◽  
Resting Cells ◽  
Negative Bacterium ◽  
Simple Method ◽  
Alginate Gel ◽  
The Stability ◽  
Model Oil ◽  
Pseudomonas Delafieldii ◽  
Ca Alginate ◽  
Desulfurization Activity

Gram-negative bacterium, Pseudomonas delafieldii R-8 (CGMCC 0570) is capable of desulfurizing dibenzothiophene (DBT) and 4,6-dimethyldibenzothiophene (4,6-DMDBT) to produce corresponding monohydroxydimethylbiphenyl. The immobilization of the resting cells of this strain in Ca-alginate gel effectively improved the stability of the cells and the desulfurization ability per amount of cells. 1 mmol/L of DBT and 4,6-DMDBT could be completely degraded in about 1 d. About 39 percent of the activity for 4,6-DMDBT was recovered after immobilization. The desulfurization activity was increased with the decrease of the diameter of the beads. The Ca-alginate immobilized cells could be used repeatedly for over 190 h with the addition of calcium ions to strengthen them. A thin layer of hydrophobic polyurea was coated on the surface of Ca-alginate gel using a simple method. The desulfurization activity was enhanced after the coating.

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