Antioxidant Activity of Schisandra Chinensis Extract and Derivatives

2012 ◽  
Vol 610-613 ◽  
pp. 3549-3551
Author(s):  
Jing Chun Wang ◽  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Methyl Ethyl Ketone ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Schisandra Chinensis ◽  
Methyl Ethyl ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene

Antioxidant activities of acetone and methyl ethyl ketone extracts from leaves of Schisandra chinensis, one of traditional Chinese medicine consumed in the eastnorth region of China, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, methyl ethyl ketone extracts and derivative were superior to that of control with IC50 were 256.54, 342.28 and 124.32μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 278.29, 384.12 and 145.25μg/mL. It was concluded that Schisandra chinensis and its derivatives might be a potential natural source of antioxidants.

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

Antioxidant Activity of Acanthopanax senticosus Extract and Derivatives

2012 ◽  
Vol 531 ◽  
pp. 524-527
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acanthopanax Senticosus ◽  
Methanol Extracts ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Radical Scavenging Assay

Antioxidant activities of acetone and methanol extracts from leaves of Acanthopanax senticosus, one of traditional Chinese medicine consumed in the eastnorth region of China, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, methanol extracts and derivative were superior to that of control with IC50 were 298.58, 360.16 and 142.21μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 305.37, 400.39 and 156.69μg/mL. It was concluded that Acanthopanax senticosus and its derivatives might be a potential natural source of antioxidants.

In Vitro Antioxidant Activity of Camellia japonica L

2012 ◽  
Vol 518-523 ◽  
pp. 5555-5558
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Camellia Japonica ◽  
Methanol Extracts ◽  
Ic50 Values ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene

Antioxidant activities of acetone and methanol extracts from stems and leaves of Camellia japonica L, one of traditional Chinese medicine consumed in the eastnorth region of China, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In the DPPH system, the antioxidant activity of the acetone extracts was superior to that of the methanol extracts, with IC50 values were 246.56 and 320.17μg/mL, respectively. In the β-carotene-linoleic acid test, the inhibition ratio of the acetone extracts was found to be the higher, compared with the methanol extracts, with IC50 values were 258.19 and 396.88 μg/mL In general, the methanol extract showed greater activity than the acetone extract in both systems, such results might be attributed to the effects of the components of the methanol extract. It was concluded that Camellia japonica L might be a potential natural source of antioxidants.

Incorporation of Cornus mas L. in Soybean Oil: Evaluation of Phytochemical and Antioxidant Activity

2020 ◽  
Vol 16 (3) ◽  
pp. 397-402 ◽  
Author(s):  
Nabi Shariatifar ◽  
Massoud Amanlou ◽  
Ramin Rahimnia ◽  
Shabnam Mahernia ◽  
Amin M. Khaneghah
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Soybean Oil ◽  
Antioxidant Activities ◽  
Beta Carotene ◽  
Dpph Assay ◽  
Synthetic Antioxidant ◽  
Cornus Mas ◽  
Peroxide Values ◽  
Β Carotene

Background: The active compounds of Cornus mas L. were extracted by the aid of water and ethanol and were incorporated into soybean oil samples. Afterward, their antioxidant activities were examined. Methods: The 1, 1 diphenyl-2- picrylhydrazyl (DPPH) and beta-carotene methods were approached in order to assess the antioxidant capacity. Different concentrations of extracts prepared (0,200, 400 and 800 mg/lit) and β hydroxyl toluene (BHT, 100 mg/lit) were added into soybean oil, and the resulted mixtures were incubated for 35 days at 65°C. Peroxide values (PVs) and thiobarbituric acidreactive substances (TBARs) levels were measured each week during the incubation. The collected data from each incubation was analyzed using ANOVA test. Results: Results demonstrated that corresponded values for β-carotene-linoleic acid and DPPH assay of the Cornus mas L. extracted by ethanol and water were lower than synthetic antioxidant and BHT. Conclusion: PVs and TBARs levels of soybean oil during the incubation time was decreased by the incorporation of extracts (water and ethanol) of Cornus mas L. while compared to the control.

scholarly journals In-Vitro Antioxidant Activity and Total Phenolic Content of Ruta montana L. Extracts

2020 ◽  
Vol 10 (2) ◽  
pp. 69-75
Author(s):  
Mounira Merghem ◽  
Saliha Dahamna
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Ruta Montana ◽  
Β Carotene

The aim of this study is to evaluate in vitro antioxidant activities of Ruta montana L.  extracts. This activity was evaluated by three methods : DPPH (2, 2'-diphenyl- 1- picrylhydrazy), bleaching of β-carotene and chelation of ferrous iron. Results showed that ethyl acetate extract (EAE) represents the highest amount of total polyphenols, tannins and flavonoids with 257,1 ± 0,703µg gallic acid equivalent/mg of extract,  251 ± 1.41 µg tannic acid equivalent /mg of extract,117,4 ± 3,451 µg quercetin equivalents/mg of extract, 139,5 ± 4,107 µg rutin equivalents/mg of extract, respectively. In the DPPH assay, ethyl acetate extract showed the higher scavenging capacity (IC50 = 0.044 ± 0.001 mg/ml) followed by methanol, aqueous and chloroform extract. Whereas, AqE showed the best chelating effect and the best inhibitory capacity of the coupled oxidation of linoleic acid/ β-carotene. Keywords: Ruta montana L; polyphenols; antioxidant activity; free radical scavenging.

Antioxidant and Phenolic Characterization with HPLC of Various Extract of Verbascum glomeratum Linneus

2020 ◽  
Vol 42 (2) ◽  
pp. 222-222
Author(s):  
i dem Aydin i dem Aydin ◽  
Akgul Rakhimzhanova Akgul Rakhimzhanova ◽  
zge Kilin arslan and Ramazan Mammadov zge Kilin arslan and Ramazan Mammadov
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Phenolic Constituents ◽  
Ethanol Extracts ◽  
Β Carotene

Verbascum L. species have been used since ancient times in traditional medicine thanks to their bioactive compounds. In this study, we evaluated the antioxidant activity and phenolic contents of ethanolic and acetonic extract of Verbascum glomeratum that collected from Denizli, Turkey. The antioxidant activity were evaluated by using various methods (β-carotene/linoleic acid, ferric reducing power assays (FRAP), radical scavenging assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2and#39;-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS), phoshomolibdenum methods) and total phenolic, flavonoid and tannin contents in the extracts were determined. Antioxidant activity was determined as β-carotene/linoleic acid (%58.5and#177;0.021), DPPH (IC50: 1.220and#177;0.025) and ABTS (IC50:1.552and#177;0.021), ferric reducing antioxidant power (FRAP) (4.151and#177;0.056 mgTE/g), phoshomolibdenum assay (8.218and#177;0.015 mgAE/g) total phenolic, total flavonoid and tannin contents was found as (4.41and#177;0.007 mgGAE/mL), (87.142and#177;0.009 and#181;QE/g), and (30.178and#177;0.008 mgCEs/g) respectively, and then 14 different phenolic compounds in ethanol extracts was carried out by HPLC. Epicatechin (2742.09 and#181;g/g) and 2,5dihidroksi (2544.96 and#181;g/g) were the most abundant phenolic constituents in the extracts. According the results, the extract of V. glomeratum may be considered as a potential source of biological agents.

Phytochemical Profile, Antimicrobial and Antioxidant Activities of Cistus clusii Essential oil From Algeria

2020 ◽  
Vol 16 (2) ◽  
pp. 109-114
Author(s):  
Souad Khebri ◽  
Naima Benkiki ◽  
Mouloud Yahia ◽  
Hamada Haba
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Β Carotene

Objective: In this work, we investigated the chemical composition and the antioxidant and antimicrobial activities of the Algerian Cistus clusii Dunal essential oil. Methods: Cistus clusii essential oil has been extracted using hydrodistillation method and characterized by GC/MS. The antioxidant activity was evaluated by two different methods DPPH free radical scavenging, and β-carotene bleaching test. The antimicrobial activity was carried out using disc diffusion method against reference strains; Pseudomonas aeruginosa (ATCC 27865), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), clinical bacterial (Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus) and fungal (Candida albicans) isolates. Results: The chemical profile of the oil showed 31 compounds. This profile was dominated by the presence of monoterpene hydrocarbons (78.4%), oxygenated derivatives (18.8%) and sesquiterpene hydrocarbons as minor constituents of this essential oil (1.9%). The major constituents were Camphene (20.6%), γ-Terpinene (16.5%), Sabinene (12.8%), and Terpineol-4 (13.5%). Other compounds were found in moderately good amounts like α-Terpinene (8.9%), α-Thujene (5.9%), α-Pinene (4.2%), and α- Terpinolene (3.1%). The results of the antimicrobial activity showed that the highest activity was observed against C. albicans and it was higher than positive control Fluconazole. The essential oil exhibited a strong antioxidant activity, especially in the β-carotene system. Conclusion: This study indicates that Cistus clusii essential oil showed potent antioxidant and moderate antimicrobial activities which could be used to explain the use of this species in traditional medicine.

scholarly journals Chemical Compositions, Antioxidant and Antimicrobial Activity of the Essential Oils of Piper officinarum (Piperaceae)

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Wan Mohd Nuzul Hakimi Wan Salleh ◽  
Farediah Ahmad ◽  
Khong Heng Yen ◽  
Hasnah Mohd Sirat
Keyword(s):  
Linoleic Acid ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Chemical Compositions ◽  
Weak Activity ◽  
Lipid Peroxidation Inhibition ◽  
Β Carotene

This study was designed to investigate the antioxidant and antimicrobial activities of the essential oils from Piper officinarum C. DC. GC and GC/MS analysis of the leaf and stem oils showed forty one components, representing 85.6% and 93.0% of the oil, respectively. The most abundant components in the leaf oil were β-caryophyllene (11.2%), α-pinene (9.3%), sabinene (7.6%), β-selinene (5.3%) and limonene (4.6%), while β-caryophyllene (10.9%), α-phellandrene (9.3%), linalool (6.9%), limonene (6.7%) and α-pinene (5.0%) were the main components of the stem oil. The antioxidant activities were determined by using complementary tests: namely β-carotene-linoleic acid, DPPH radical scavenging and total phenolic assays. The stems oil showed weak activity (IC50 = 777.4 μg/mL) in the DPPH system, but showed moderate lipid peroxidation inhibition in the β-carotene-linoleic acid system (88.9 ± 0.35%) compared with BHT (95.5 ± 0.30%). Both oils showed weak activity against P. aeruginosa and E. coli with M IC values of 250 μg/mL.

scholarly journals Characterization of Bioactive Compounds, Antioxidant Properties and Antimicrobial Activity of Red And White Cabbage Leaves Extracts

2018 ◽  
Vol 2 (1) ◽  
Keyword(s):  
Linoleic Acid ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Polyphenols ◽  
Red Cabbage ◽  
White Cabbage ◽  
Β Carotene

Cabbage is known as a rich source of bioactive compounds including carotenoids and phenolic compounds which may have antibacterial and antioxidant properties. This investigation was undertaken to estimate the effect of using different organic solvents on the total polyphenols content, antibacterial and antioxidant capacities of red and white cabbage. Phenolic compounds analysis was performed by Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) and antioxidant activities of cabbage were evaluated using DPPH radical scavenging and β-carotene-linoleic acid bleaching.Methanol has the highest extraction yields of 23.83 and 22.05 %. Alcoholic extracts from red cabbage exhibit the highest content of total polyphenols (205.66-190.77 mg GAE/ 100g DW) and flavonoids (137.26-123.6 mg CE/100g DW) meanwhile; white cabbage had the highest content of β-carotene and chlorophyll. Red cabbage exhibited the highest DPPH scavenging value (IC50= 257, 80 μg/ml) and the highest β-carotene-linoleic acid bleaching (73.85%). Significant correlations were found between antioxidant activity of extracts from cabbage samples and the contents of polyphenols and flavonoids. The studied extracts didn’t show an antibacterial activity against tested germs.

scholarly journals CHEMICAL COMPOSITION OF ESSENTIAL OIL AND ANTIOXIDANT ACTIVITY OF Postia puberula, AN ENDEMIC SPECIES FROM IRAN

2019 ◽  
Vol 18 (1) ◽  
pp. 119-128
Author(s):  
Parvaneh Hemmati Hassan Gavyar ◽  
Hamzeh Amiri
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Chemical Composition ◽  
Dibutyl Phthalate ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Caryophyllene Oxide ◽  
Flowering Stage ◽  
Benzyl Benzoate ◽  
Β Carotene

Essential oils from the leaves, stems and flowers of Postia puberula at the flowering stage were analyzed using GC and GC/MS. The results showed that leaves are abundant of cis-3-hexenyl benzoate (10.75%), benzyl benzoate (8.16%) and caryophyllene oxide (8.12%). The main compounds of stems were benzyl benzoate (21.92%), E-nuciferol (11.58%) and dibutyl phthalate (7.08%), while major components of flowers were benzyl benzoate (9.99%), caryophyllene oxide (8.14%) and E-nuciferol (8.13%). The antioxidant activities of methanol extract were evaluated by DPPH and β-carotene/linoleic acid assays. The results showed that in both methods, leaves had stronger antioxidant activity than other organs.

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