Preliminary Experiment on Extraction of Eisenia foetida Coelomic Fluid with Water Bath Pot

2012 ◽  
Vol 610-613 ◽  
pp. 3566-3569
Author(s):  
Shao Ming Zhuo ◽  
Jia Xie ◽  
Chun Yuan Wu ◽  
Guang Yi Li
Keyword(s):  
Maximum Amount ◽  
Water Bath ◽  
Extraction Time ◽  
Generation Rate ◽  
Coelomic Fluid ◽  
Eisenia Foetida ◽  
Extraction Temperature ◽  
Diluted Solution ◽  
Optimum Extraction

This work explored a new water bath stimulating method to extract coelomic fluid from active Eisenia foetida. Coelomic fluid discharge amount and the coelomic fluid activity were tested to obtain the optimum extraction temperature (ET) and time. The maximum amount of coelomic fluid was obtained at 42 oC, at which vermis is exact activity, while the vermis activity is inhibited at 44 oC and even the vermis comprehensive death is observed at 46 oC, therefore the optimum ET is 42 oC; the optimum extraction time is 7 a.m. and 6 p.m for which the volume of coelomic fluid is the most. When extracted from coelomic fluid at 7 a.m and 11a.m, 60 times and 10 times diluted solution obtained the highest generation rate; when it is at 3 p.m., 10 and 100 dilution times is the best; when it is at 1 p.m., 30 and 200 dilution times is the best, and 30-80 dilution times is the optimal when it is from 3 p.m..

scholarly journals Study on Extraction Technology of Pitaya Polysaccharide

2021 ◽  
Vol 251 ◽  
pp. 02052
Author(s):  
Feng Xuehua ◽  
Song Zurong ◽  
Tao Ali ◽  
Gong Panpan
Keyword(s):  
Orthogonal Test ◽  
Raw Material ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Single Factor ◽  
Liquid Ratio ◽  
Extraction Technology ◽  
Solid Liquid ◽  
Optimum Extraction

Using pitaya as raw material, this paper used ethanol subsiding method to study the extraction technology of pitaya polysaccharide by single factor test and orthogonal test. Through orthogonal test, we have found the optimum extraction conditions of pitaya polysaccharide as follows: extraction temperature 60℃, solid-liquid ratio 1:30, extraction time 3h. Under the optimal extraction conditions, the yield of pitaya polysaccharide was 1.13%.

Optimization of Ultrasonic Extraction Process by Response Surface Methodology of Polysaccharides from Limonium bicolor Kunze (Bge.)

2011 ◽  
Vol 317-319 ◽  
pp. 321-324
Author(s):  
Peng Zhao ◽  
Ou Li ◽  
A Ping Liu
Keyword(s):  
Extraction Process ◽  
Ultrasonic Extraction ◽  
Raw Material ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Limonium Bicolor ◽  
Predicted Values ◽  
Optimum Extraction ◽  
Extraction Power

Polysaccharide from Limonium bicolor Kunze (Bge.)(LBPs) was extracted with the assistance of ultrasonic.Box-Behnken design(BBD) was employed to optimize extraction time; extraction temperature;ultrasonic extraction power and ratio of water to raw material to obtain a high LBPs yield.The optimum extraction conditions were as follows: extraction temperature 81.23°C, ultrasonic power 190.86W, extraction time 42.39 min and ratio of water to raw material 15.4ml/g.The yield of polysaccharide was 7.11% based on the above mentioned conditions. Close agreement between experimetal and predicted values was found.

Optimization of Process for Extraction of Yellow Pigment from Almond Shell

2014 ◽  
Vol 522-524 ◽  
pp. 429-432
Author(s):  
Feng Jie Wang
Keyword(s):  
Extraction Yield ◽  
Alcohol Concentration ◽  
Yellow Pigment ◽  
Orthogonal Test ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Optimum Conditions ◽  
Almond Shell ◽  
Extraction Agent ◽  
Optimum Extraction

The effect of extraction temperature,extraction time,alcohol concentration, ratio of material to liquid (g/ml)on extraction yield of natural yellow pigment from waste almond shell were studied. The optimum conditions were determined by L9 (34) the orthogonal test . The optimum extraction conditions are the extraction temperature at 75°C;the extraction time for 2h; the extraction agent concentration of 20%ethanol ; the material-liquid ration (g/ml) 1:15.The extraction yield of natural yellow pigment from almond shell is 6.25%,The order of effects are extraction temperature>extraction time>ratio of material to liquid(g/ml)>alcohol concentration.This research provides the theoretical basis for the industrial production of the extraction of natural almond shell pigment and provides a new way for the re-use of the abandoned almond shell.

scholarly journals Optimization of extraction technology of polysaccharide from foxtail millet using response surface methodology

2014 ◽  
Vol 20 (4) ◽  
pp. 579-585 ◽  
Author(s):  
Ai-Shi Zhu ◽  
Jin-Na Ye ◽  
Fei-Na Yan
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Foxtail Millet ◽  
Polynomial Equation ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Extraction Technology ◽  
Solid Ratio ◽  
Experimental Yield ◽  
Optimum Extraction

The experiment of extraction of polysaccharide from foxtail millet was investigated. Response Surface Methodology (RSM),based on a threelevel, three variablesBox-Behnken design (BBD), was employed to obtain the best possible combination of liquid-solid ratio(X1: 15.0-25.0 mL?g-1),extraction time (X2: 1.5-2.5h), and extraction temperature (X3: 65.0-75.0?C)for maximum polysaccharide yields. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods (ANOVA). The optimum extraction conditions were as follows: liquid-solid ratio 20.7mL?g-1, extraction time 2.0h, extraction temperature 72.3?C. Under these conditions, the experimental yield was 8.08mg?g-1, which is well in close agreement with8.02mg?g-1predicted value by the model.

Optimization of Ultrasonic Extraction Process by Response Surface Methodology of Polysaccharides from Tussilago farfara L.

2013 ◽  
Vol 771 ◽  
pp. 41-44
Author(s):  
Peng Zhao ◽  
Xiao Song
Keyword(s):  
Response Surface Methodology ◽  
Extraction Process ◽  
Ultrasonic Extraction ◽  
Raw Material ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Tussilago Farfara ◽  
Optimum Extraction ◽  
Extraction Power

Polysaccharides from Tussilago farfara L.(TFPs) was extracted with the assistance of ultrasonic.Box-Behnken design (BBD) was employed to optimize extraction time; extraction temperature;ultrasonic extraction power and ratio of water to raw material to obtain a high TFPs yield.The optimum extraction conditions were as follows: extraction temperature 65.1°C, ultrasonic power 380.5W, extraction time 28.7 min and ratio of water to raw material 23.6ml/g.The yield of polysaccharide was 2.14% based on the above mentioned conditions.

Study on Extraction of Natural Dyestuff from Lawsonia inermis

2012 ◽  
Vol 550-553 ◽  
pp. 1807-1810
Author(s):  
Hong Li ◽  
Rui Liu
Keyword(s):  
Extraction Time ◽  
Extraction Temperature ◽  
Optimal Process ◽  
Lawsonia Inermis ◽  
Extraction Solvent ◽  
Optimum Extraction

The optimal process of extracting the natural dyestuff from Lawsonia inermis were studied. Results showed that the optimum extraction conditions are as follows: extraction solvent is 60% ethanol, the ratio between material and solvent is 1:20 (W/V), extraction temperature is 75°Cand extraction time is 120min.

scholarly journals Skin-Whitening and Anti-Wrinkle Effects of Bioactive Compounds Isolated from Peanut Shell Using Ultrasound-Assisted Extraction

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1231
Author(s):  
Da Hye Gam ◽  
Ji Woo Hong ◽  
Jun Hee Kim ◽  
Jin Woo Kim
Keyword(s):  
Ethanol Concentration ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Peanut Shell ◽  
Ultrasound Assisted Extraction ◽  
Skin Whitening ◽  
Activity Inhibition ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Experimental Values

Response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) conditions for simultaneous optimization of dependent variables, including DPPH radical scavenging activity (RSA), tyrosinase activity inhibition (TAI), and collagenase activity inhibition (CAI) of peanut shell extracts. The effects of the main variables including extraction time (5.0~55.0 min, X1), extraction temperature (26.0~94.0 °C, X2), and ethanol concentration (0.0%~99.5%, X3) were optimized. Based on experimental values from each condition, quadratic regression models were derived for the prediction of optimum conditions. The coefficient of determination (R2) of the independent variable was in the range of 0.89~0.96, which demonstrates that the regression model is suitable for the prediction. In predicting optimal UAE conditions based on the superimposing method, extraction time of 31.2 min, extraction temperature of 36.6 °C, and ethanol concentration of 93.2% were identified. Under these conditions, RSA of 74.9%, TAI of 50.6%, and CAI of 86.8% were predicted, showing good agreement with the experimental values. A reverse transcription polymerase chain reaction showed that peanut shell extract decreased mRNA levels of tyrosinase-related protein-1 and matrix metalloproteinase-3 genes in B16-F0 cell. Therefore, we identified the skin-whitening and anti-wrinkle effects of peanut shell extracts at protein as well as gene expression levels, and the results show that peanut shell is an effective cosmetic material for skin-whitening and anti-wrinkle effects. Based on this study, peanut shell, which was considered a byproduct, can be used for the development of healthy foods, medicines, and cosmetics.

scholarly journals Homogenate Extraction of Crocins from Saffron Optimized by Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yingpeng Tong ◽  
Yu Jiang ◽  
Dan Guo ◽  
Yongqiu Yan ◽  
Shiping Jiang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Process Conditions ◽  
Extraction Temperature ◽  
Single Factor ◽  
Extraction Voltage ◽  
Homogenate Extraction

Saffron, which has many kinds of biological activities, has been widely used in medicine, cosmetics, food, and other fields of health promotion industries. Crocins are the main component of saffron (Crocus sativus L.). At present, most of the extraction methods for crocins require long time or special instruments to complete the process and some of them are not suitable for industrial production at present. In this article, homogenate extraction technology which is a convenient and efficient method was developed for crocins extraction from saffron. Firstly, the influences of extraction voltage, extraction time, ethanol concentration, and temperature on crocins yield were studied by single factor experiments; and then response surface methodology (RSM) was used to optimize levels of four variables based on the result of single factor experiments. Results showed that the optimum extraction process conditions for crocins were as follows: extraction voltage, 110 V; ethanol concentration, 70%; extraction temperature, 57°C; and extraction time, 40 s. Based on these conditions, the extraction yield of crocins can reach 22.76% which is higher than ultrasonic extraction method. Therefore, homogenate extraction is an effective way to extract crocins from saffron with higher extraction yield and shorter extraction time.

E10 4:30 A mitogenic factor of the coelomic fluid of Eisenia foetida earthworms

1997 ◽  
Vol 21 (2) ◽  
pp. 117
Author(s):  
Radka Hanusová ◽  
Alain Beschin ◽  
Martin Bilej ◽  
Patrick De Baetselier
Keyword(s):  
Coelomic Fluid ◽  
Eisenia Foetida ◽  
Mitogenic Factor

Microwave-Assisted Extraction of Chlorophyll from Filter Mud of Sugercane Mill and Component Analysis

2012 ◽  
Vol 518-523 ◽  
pp. 430-435 ◽  
Author(s):  
Hai Rong Guo ◽  
Shao Ying Ma ◽  
Xiao Fei Wang ◽  
Er Fang Ren ◽  
Yuan Yuan Li
Keyword(s):  
Irradiation Time ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Microwave Assisted Extraction ◽  
Optimal Conditions ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Conventional Extraction ◽  
Filter Mud ◽  
Strong Absorption Peak

Microwave-assisted extraction (MAE) was used to extract chlorophylls from filter mud. Ethanol was used as the solvent. The optimal conditions for the MAE of chlorophylls were concluded from the study as the irradiation time, 50 s, the ratio of liquid to solid, 8:1 (mL/g), the extraction temperature, 40 °C, and the extraction time, 60 min. Compared with conventional extraction, the MAE of chlorophylls from the filter mud was more effective. The extraction time for MAE was 60 min with 0.277 mg/g chlorophyll yield, while conventional extraction needed 240 min with only about 0.259 mg/g chlorophyll yield. The Ultraviolet Absorption Spectra of the extracted chlorophylls showed that there was a strong absorption peak at about 663 nm. C=N, Mg-N and C-N was not seen existed from the infrared spectroscopy probably because that the mixed extracts were not purified and the chlorophyll content was less.

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