Development of a Highly Sensitive Chemiluminescence Enzyme Immunoassay for the Determination of Bisphenol A

2013 ◽  
Vol 726-731 ◽  
pp. 1283-1286
Author(s):  
Li Rui Liu ◽  
Li Qin Liu ◽  
Xue Qing Chen ◽  
Guo Qing Shi
Keyword(s):  
Detection Limit ◽  
Bisphenol A ◽  
Enzyme Immunoassay ◽  
Rapid Determination ◽  
Secondary Antibody ◽  
Average Recovery ◽  
Highly Sensitive ◽  
Optimized Conditions ◽  
Chemiluminescence Enzyme Immunoassay

A highly sensitive chemiluminescence enzyme immunoassay (CLEIA) method for the determination of bisphenol A (BPA) was developed, which used a secondary antibody labeled with horseradish peroxidase detected with a luminol-based substrate. Under the optimized conditions, the linear range was 0.01μg/mL~0.74μg/mL, and the detection limit was 0.008μg/mL. The average recovery for BPA in barreled water was 104%. This developed method could be applied for the selective, high-throughput, and rapid determination of BPA in barreled water.

Selective Determination of Microcystin-LR Based on Proteasomal Trypsin-Like Activity Inhibition

2012 ◽  
Vol 518-523 ◽  
pp. 5594-5597
Author(s):  
Xiao Shen Lu ◽  
Shi Feng Li ◽  
Li Zhang ◽  
Xin Yu Guo ◽  
Guo Qing Shi
Keyword(s):  
Detection Limit ◽  
Rapid Determination ◽  
Tap Water ◽  
Selective Method ◽  
Average Recovery ◽  
Selective Determination ◽  
Inhibiting Effect ◽  
Activity Inhibition ◽  
Optimized Conditions

A sensitive and selective method was developed for the determination of microcystin-LR (MC-LR) based on proteasomal trypsin-like activity inhibition. Under the optimized conditions, the detection limit for MC-LR was 0.18μg/L, The linearity range was 0.2-2 μg/L，and the average recovery for MC-LR in tap water was 128%. Microcystin-YR (MC-YR) and Microcystin-RR (MC-RR) have no inhibiting effect on the proteasomal trypsin-like activity in the concentration ranged between 0.1-100 nmol/L. This method could be applied for the selective, high-throughput, and rapid determination of MC-LR in waters.

Determination of Salbutamol, Clenbuterol, and Brombuterol in Urine by a Highly Sensitive Chemiluminescence Enzyme Immunoassay

2014 ◽  
Vol 47 (16) ◽  
pp. 2761-2773 ◽  
Author(s):  
Yuping Wu ◽  
Fei Xu ◽  
Haiyang Jiang ◽  
Xiaoqi Tao ◽  
Kui Zhu ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Highly Sensitive ◽  
Chemiluminescence Enzyme Immunoassay

Liquid Chromatographic Determination of Sulfamoyldapsone in Swine Tissues

1987 ◽  
Vol 70 (6) ◽  
pp. 1031-1032
Author(s):  
Yuuko S Endoh ◽  
Ryozo Yamaoka ◽  
Nobuo Sasaki
Keyword(s):  
Detection Limit ◽  
Quantitative Determination ◽  
Chromatographic Determination ◽  
Alumina Column ◽  
Average Recovery ◽  
Diaminodiphenyl Sulfone ◽  
Alumina Column Chromatography ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method is described for the quantitative determination of sulfamoyldapsone (2-sulfamoyl-4,4'-diaminodiphenyl sulfone) in swine muscle, liver, kidney, and fat. Sulfamoyldapsone was extracted from tissues with acetonitrile saturated with n-hexane. The extract was washed with n-hexane saturated with acetonitrile, concentrated, and cleaned up by alumina column chromatography. Sulfamoyldapsone was separated on an ODS column by using acetonitrile-methanol-water (6 + 18 + 76) and was detected at 292 nm. Overall average recovery of sulfamoyldapsone added to tissues at levels of 0.1 and 0.5 /μg/g was 93.3% ± 6.0. Detection limit was 0.02 μg/g in these tissues.

scholarly journals Selective Determination of Iron(III) in Sea Water by Gold Nanoparticles Self-assembled with N-carboxyl- L-cysteine

2014 ◽  
Vol 17 (1) ◽  
pp. 001-004 ◽  
Author(s):  
Benzhi Liu ◽  
Min Wang
Keyword(s):  
Gold Nanoparticles ◽  
Detection Limit ◽  
Sea Water ◽  
Stripping Voltammetry ◽  
Differential Pulse ◽  
Operational Parameters ◽  
Selective Determination ◽  
Self Assembled ◽  
Optimized Conditions

Application of gold nanoparticles self-assembled with N-carboxyl- L-cysteine for the determination of iron(III) was investigated. Differential pulse adsorptive stripping voltammetry was used to detect iron(III). Various operational parameters were investigated and discussed in terms of their effects on the measurement signals. A linear range from 0.1 nM to 1.8 nM with a detection limit of 0.03 nMwas obtained under optimized conditions. The applicability of the method was successfully tested by determination of iron(III) in sea water samples.

Liquid Chromatographic Determination of Bromide Ion in Cereals, Fruit, Vegetables, and Blood with a Silver Electrode in an Electrochemical Detector System

1995 ◽  
Vol 78 (3) ◽  
pp. 841-845 ◽  
Author(s):  
Bertil Lindgren ◽  
Tomas Berglöf ◽  
Åsa Ramberg ◽  
Anna Stepdmska ◽  
Malin Åkerblom
Keyword(s):  
Liquid Chromatography ◽  
Detection Limit ◽  
Rapid Determination ◽  
Chromatographic Determination ◽  
Electrochemical Detector ◽  
Satisfactory Precision ◽  
Bromide Ion ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method is presented for rapid determination of bromide ion in commodities and blood by paired-ion liquid chromatography with electrochemical detection. The method involves extraction of samples with water and filtration. Blood is passed through a Sep-Pak C18 minicolumn. Recoveries are usually close to 100%, with satisfactory precision. The detection limit is 1 mg/kg. The method needs little labor and uses no noxious solvents or reagents.

Sign in / Sign up

Export Citation Format

Share Document