Separation and Manipulation of Particles Using Traveling Wave Dielectrophoretic Force

In this work, we present a device for cell manipulation and separation by using travelling wave dielectophoretic force. The device consists of a 16 parallel electrode array and microchamber. The dielectrophoretic PDMS chamber was fabricated by using standard microfabrication techniques. The Cr/Au parallel electrode array of 100 µm wide and 300 nm thick was patterned on a glass slide by sputtering through microshadow mask. The polystyrene microspheres suspension in de-ionized water and red blood cells in D-mannitol solution were used as tested cells. Cells respond to the electric field in various mechanisms depending on the applied voltage and frequency of the AC signals. For 4.5 µm polystyrene, the traveling wave dielectrophoresis happened when the applied voltage was 10 V, and the frequency of the applied signals was in the range of 50 kHz-700 kHz. For 10 µm polystyrene the twDEP occurred when the applied voltage was 7 V, and frequency was in the range 30 kHz-1MHz. While the red blood cells experienced the twDEP when the applied voltage was 3 V and frequency was in the range 50 kHz-2MHz. The mixed solution containing equal amount of 4.5 and 10 µm microspheres were used for separation test. The big microspheres were moved under twDEP force when the applied voltage was 7 V, and the frequency was in the range of 25 kHz-1MHz while the small microspheres were attached to the electrodes. Therefore, the twDEP device can manipulate and separate the microspheres with different sizes, and it can be further applied for cells selection.

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A Traveling-Wave Dielectrophoresis Bio-Chip for Cell Manipulation in Standard CMOS Process

IEEE Transactions on Circuits & Systems II Express Briefs ◽

10.1109/tcsii.2021.3102661 ◽

2021 ◽

pp. 1-1

Author(s):

Yun-Sheng Chan ◽

Ting-Ting Hsu ◽

Yi-Po Chen ◽

Hsu-Min Wu ◽

Yun-Ming Wang ◽

...

Keyword(s):

Traveling Wave ◽

Cell Manipulation ◽

Cmos Process ◽

Standard Cmos Process ◽

Traveling Wave Dielectrophoresis

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High-resolution cell manipulation for longstanding load on red blood cells

2016 IEEE International Conference on Mechatronics and Automation ◽

10.1109/icma.2016.7558684 ◽

2016 ◽

Cited By ~ 1

Author(s):

Chia-Hung Dylan Tsai ◽

Mitsuhiro Horade ◽

Hiroaki Ito ◽

Makoto Kaneko ◽

Motomu Tanaka

Keyword(s):

High Resolution ◽

Red Blood Cells ◽

Blood Cells ◽

Cell Manipulation ◽

Resolution Cell

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An Analytical Method for Dielectrophoresis and Traveling Wave Dielectrophoresis Generated by an n-Phase Interdigitated Parallel Electrode Array

Journal of Fluids Engineering ◽

10.1115/1.2956610 ◽

2008 ◽

Vol 130 (8) ◽

Cited By ~ 4

Author(s):

Hongjun Song ◽

Dawn J. Bennett

Keyword(s):

Boundary Condition ◽

Analytical Solution ◽

Boundary Conditions ◽

Analytical Method ◽

Traveling Wave ◽

Electrode Array ◽

Accurate Analysis ◽

Exact Boundary ◽

Latex Beads ◽

Parallel Electrode

In this paper, we present an analytical method for solving the electric potential equation with the exact boundary condition. We analyze the dielectrophoresis (DEP) force with an n-phase ac electric field periodically applied on an interdigitated parallel electrode array. We compare our analytical solution with the numerical results obtained using the commercial software CFD-ACE. This software verifies that our analytical method is correct for solving the problem. In addition, we compare the analytical solutions obtained using the exact boundary conditions and the approximate boundary conditions. The comparison shows that the analytical solution with the exact boundary condition gives a more accurate analysis for DEP and traveling wave DEP forces. The DEP forces of latex beads are also investigated with different phase arrays for (n=2,3,4,5,6).

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Separation of Micro Particles and Biological Cells Inside an Evaporating Droplet Using Dielectrophoresis

ASME 5th International Conference on Nanochannels, Microchannels, and Minichannels ◽

10.1115/icnmm2007-30018 ◽

2007 ◽

Author(s):

Jung-Yeul Jung ◽

Ho-Young Kwak

Keyword(s):

Silicon Dioxide ◽

Red Blood Cells ◽

Applied Voltage ◽

Blood Cells ◽

Water Solution ◽

Biological Cells ◽

Silicon Dioxide Layer ◽

Electrical Force ◽

E Coli ◽

Micro Particles

Micro particles or biological cells mixed in water solution was shown to be separated using the combined electrical force due to dielectrophoresis and mechanical one generated in an evaporating droplet. Micro patterned electrodes of Au were fabricated on the silicon dioxide layer and were used for generating dielectrophoresis. Polystyrene particles, red blood cells and E-coli were used for separating objects. Micro particles and biological cells were separated by adjusting the amplitude and frequency of the applied voltage. The mechanical force is enough to transport the particles to the boundary of the droplet, nevertheless it could not detach the particles trapped at the electrode. Based on this work, the micro particles and biological cells can be separated, controlled and sensed without liquid pumping unit.

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Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100083266 ◽

1978 ◽

Vol 36 (2) ◽

pp. 480-481

Author(s):

Kosuke Ueda ◽

Hiroto Washida ◽

Nakazo Watari

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Renal Cortex ◽

Uranyl Acetate ◽

Osmic Acid ◽

Renal Lithiasis ◽

Electron Microscopic ◽

Hemoglobin C ◽

First Case ◽

Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

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Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072083 ◽

1973 ◽

Vol 31 ◽

pp. 328-329

Author(s):

John A. Trotter

Keyword(s):

Red Blood Cells ◽

Analytical Method ◽

Blood Cells ◽

Guinea Pigs ◽

Specific Protein ◽

Visual Examination ◽

Hemoglobin Synthesis ◽

Peroxidatic Activity ◽

Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

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Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010012480x ◽

1992 ◽

Vol 50 (1) ◽

pp. 880-881

Author(s):

Victor Tsutsumi ◽

Adolfo Martinez-Palomo ◽

Kyuichi Tanikawa

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Red Blood Cells ◽

Entamoeba Histolytica ◽

Causative Agent ◽

Blood Cells ◽

Protozoan Parasite ◽

Complex Phenomenon ◽

Great Capacity ◽

Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

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Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162132 ◽

1990 ◽

Vol 48 (3) ◽

pp. 914-915

Author(s):

D.J.P. Ferguson ◽

A.R. Berendt ◽

J. Tansey ◽

K. Marsh ◽

C.I. Newbold

Keyword(s):

Plasmodium Falciparum ◽

Red Blood Cells ◽

Blood Cells ◽

Umbilical Vein ◽

Cell Types ◽

Amelanotic Melanoma ◽

Cytoplasmic Process ◽

Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

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