Research on Direct Effects of High-Voltage Electrostatic Field on Lactoperoxidase's Activity

At present, indirect effects of high-voltage electrostatic field on organisms and biological materials have been extensively studied. However, the investigations on direct interaction between the electrostatic field and enzyme are scarce. In this paper, the direct effect of high-voltage electrostatic field on the LP and the protective effects of sucrose fatty acid ester on the activity of enzyme in high-voltage electrostatic field have been experimentally investigated. The results show that, high-voltage electrostatic field has direct effects on the LP and the best condition to enhance the activity of enzyme can be obtained. When the electric-field strength is low, with the increasing of the electric-field strength, the activity of the LP will be weakened. When the electric-field strength exceeds a certain level, the activity of the LP will be enhanced with the increasing of electric-field strength. For too higher electric-field strength , the activity of the LP will be again weakened with the increasing of electric-field strength. The sucrose fatty acid ester in low concentration provides a proper hydrophobic microenvironment for enzyme, which leads to the enhancement of enzyme's activity, while sucrose fatty acid ester in high concentration leads to the weakening of enzyme's activity. Moreover, sucrose fatty acid ester in enzyme solutions has protective effects on enzyme in the electric field and can reduce the impacts of high-voltage electrostatic field on the LP.