Preparation of Phosphorylated Chitosan/ Chitosan/ Hydroxyapatite Composites by Co-Precipitation Method

2009 ◽  
Vol 79-82 ◽  
pp. 401-404 ◽  
Author(s):  
Bin Li ◽  
Xin Bo Wang ◽  
Jin Huan Ma ◽  
Long Nan Huang
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cancellous Bone ◽  
Bending Strength ◽  
Weight Ratio ◽  
Strength Test ◽  
Precipitation Method ◽  
Co Precipitation

In this work, the PCS/CS/HA composites with different weight ratios were prepared through a co-precipitation method. The properties of these composites were characterized by means of the XRD, the IR, the SEM and the bending strength test. The value of bending strength of the PCS/CS/ HA composite with a weight ratio of 10/30/60 was measured about 34.93 MPa which is 1.6 times high of the cancellous bone. The composite is appropriate to be used as materials for bone tissue engineering.

scholarly journals Preparation and Evaluation of Biocompatible Composite for Bone Tissue Engineering

2017 ◽  
Vol 4 (3) ◽  
pp. 7-14
Author(s):  
Masud Rana Md. ◽  
Naznin Akhtar ◽  
Zahid Hasan Md. ◽  
Asaduzzaman S M
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biomedical Application ◽  
Bone Tissue Regeneration ◽  
Precipitation Method ◽  
Tissue Engineering Scaffolds ◽  
Fixed Amount ◽  
Co Precipitation

Bone tissue engineering with cells and synthetic extracellular matrix represents a new approach for the regeneration of mineralized tissue compared with the transplantation of bone. Hydroxyapatite (HA) and its composite with biopolymer are extensively developed and applied in bone tissue regeneration. The main aim of this study was to fabricate and characterize of HA apatite based biocompatible scaffold for bone tissue engineering. Scaffolds with different ratio of polymers (chitosan & alginate), and fixed amount of synthetic HA were prepared using in situ co precipitation method and mineral to polymer ratio was 1:1 to 1: 2 . A cross linker agent, 2-Hydroxylmethacrylate (HEMA) was added at different percentage (0.5-2%) into the selected composition and irradiated at 5- 25 kGy to optimize the proper mixing of components at the presence of HEMA. Fabricated scaffolds were analyzed to determine porosity, density, biodegradability, morphology and structural properties. Porosity and density of the prepared scaffold were 75 to 92% and 0.21 to 0.42 g/cm3 respectively. However, the swelling ratio of the fabricated scaffolds was ranged from 133 to 197%. Nonetheless, there had a reasonable in-vitro degradation of prepared scaffolds. Flourier transform infrared spectroscopy (FTIR) analysis showed intermolecular interaction between components in the scaffold. Pore size of scaffold was measured by scanning electron microscope and the value was 162-510 μm. It could be proposed that this scaffold fulfills all the main requirements to be considered as a bone substitute for biomedical application in near future.

Biomimetic Composites Based on Calcium Phosphates and Chitosan - Hyaluronic Acid with Potential Application in Bone Tissue Engineering

2013 ◽  
Vol 587 ◽  
pp. 191-196 ◽  
Author(s):  
Florina Daniela Ivan ◽  
Andreea Marian ◽  
Constantin Edi Tanase ◽  
Maria Butnaru ◽  
Liliana Verestiuc
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calcium Phosphates ◽  
Degradation Process ◽  
Precipitation Method ◽  
Negative Effect ◽  
Co Precipitation

Composites based on calcium phosphates (CP) and mixtures of biopolymers (chitosan and hyaluronic acid) have been prepared by a biomimetic co-precipitation method and tested as scaffolds for bone tissue engineering. The biomimetic strategy is inspired by natural mineralization processes, where the synthesized minerals are usually combined with proteins, polysaccharides or other mineral forms to form composite, in physiological conditions of temperature and pH. Fourier transformed infrared spectroscopy, scanning electron microscopy, X-ray diffraction and XPS analyses confirmed the porous morphology of the scaffolds and formation of various forms of calcium phosphates with amorphous nature. Thein vitrodegradation studies showed a slow degradation process for CP-biopolymers composites and limited swelling in simulated body fluids. The scaffolds compositions have no negative effect on osteoblasts cell, emphasizing a good biocompatibility.

Preparation and Characterization of Fibroin/Chitosan/Hydroxyapatite Porous Scaffold

2013 ◽  
Vol 849 ◽  
pp. 151-156 ◽  
Author(s):  
Tarin Sukhachiradet ◽  
Wassanai Wattanutchariya
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
Chemical Precipitation ◽  
Precipitation Method ◽  
Xtt Assay ◽  
Average Porosity ◽  
Biocompatibility Test

Autograft is a general method used in orthopedic surgery for a bone replacement. However, the disadvantage of this method is the amount of risk factor to the donor sites. Currently, bone tissue engineering is another technique that could be implemented to solve this problem. Artificial bone scaffold generated by bone tissue engineering can be employed in order to accelerate damaged bone regeneration. In fact, this scaffold can be fabricated from synthetic contents such as bioceramics, biopolymers or composite. Three types of biomaterials: Chitosan, Hydroxyapatite (HA) and Fibroin were used to form porous scaffold. This research investigated the preparation of Hydroxyapatite and Fibroin from natural materials. Hydroxyapatite was synthesized from mollusk shell by wet chemical precipitation method. While, Fibroin was extracted from silk worms cocoons. Freeze drying method was employed to fabricate this composite porous scaffold. A mixing ratio of 1:2:1 among Fibroin: Chitosan: HA was studied to evaluate biodegradability, biocompatibility, porosity and pore structure of the output scaffolds. Results show that the output scaffolds have an interconnected porous structure with a pore size around 150-200μm and an average porosity of 94.26%. While the average degradation rate of the scaffold in lysozyme was 10.46% at 7 days. In addition, the biocompatibility test based on XTT assay test, shown that the scaffolds were non-cytotoxicity, which could be good for bone filling application in the future.

Preparation of Porous Biphasic Calcium Phosphate-Gelatin Nanocomposite for Bone Tissue Engineering

2010 ◽  
Vol 11 ◽  
pp. 67-72 ◽  
Author(s):  
L. Bakhtiari ◽  
Hamid Reza Rezaie ◽  
S.M. Hosseinalipour ◽  
Mohammad A. Shokrgozar
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Porous Structure ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate ◽  
Bending Strength ◽  
Cell Viability Assay ◽  
Hydroxy Apatite ◽  
Porous Nanocomposite

A new porous structure as a bone tissue engineering scaffold was developed by a freeze-drying method. The porous nanocomposite was prepared from Biphasic Calcium Phosphate (BCP) which was a mixture of 70% hydroxy apatite and 30%ß-TCP (ß-Tricalcium Phosphate). Porogen was naphthalene and gelatin from bovine skin type B was used as polymer. Gelatin was stabilized with EDC (N-(3-dimethyl aminopropyl)-N´-ethyl carbodiimide hydrochloride) by a cross-linking method. The scaffold was characterized by scanning electronic microscope (SEM), Fourier-Transformed Infrared spectroscopy (FTIR). The biocompatibility of this nanocomposite carried out through MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a tetrazole) cell viability assay. Also other properties of scaffold such as morphology, grain size, bending strength were investigated. Highly porous structure with interconnected porosities, good mechanical behavior and high biocompatibility with bone tissue, were benefits of this porous nanocomposite for bone tissue engineering.

Production of Bioactive Nano-Hydroxyapatite/Polysaccharide Composites for Bone Tissue Engineering

2008 ◽  
Vol 587-588 ◽  
pp. 22-26
Author(s):  
Ana L. Daniel-da-Silva ◽  
A.M. Gil ◽  
Rui N. Correia
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Freeze Drying ◽  
Mechanical Performance ◽  
Calcium Phosphates ◽  
Thermally Induced ◽  
Co Precipitation

Porous κ-carrageenan based composites with potential application in bone tissue engineering have been prepared by in situ co-precipitation of nanoparticles of calcium phosphates, followed by thermally induced gelification and freeze-drying. The scaffolds showed macroporous structure with interconnected porosity. The variation of the biopolymer concentration affected the microstructure and compressive mechanical performance of the composites. The in vitro bioactivity was assessed by soaking the composites in simulated body fluid (SBF) and the formation of an apatite layer on their surface was found.

scholarly journals Fabrication, characterization, and optimization of a novel copper-incorporated chitosan/gelatin-based scaffold for bone tissue engineering applications

Bioimpacts ◽  
2021 ◽  
Author(s):  
Azam Bozorgi ◽  
Masoud Mozafari ◽  
Mozafar Khazaei ◽  
Mansooreh Soleimani ◽  
Zahra Jamalpoor
Keyword(s):  
Tissue Engineering ◽  
Mechanical Strength ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Physicochemical Characteristics ◽  
Chemical Precipitation ◽  
Precipitation Method ◽  
Calcium Deposition ◽  
Salt Leaching ◽  
Cu Substitution

Introduction: Fabricating composite scaffolds with improved physicochemical properties as artificial microenvironments are of great interest in bone tissue engineering. Given advantageous properties of nano-hydroxyapatite/chitosan/gelatin (nHA/Cs/Gel) scaffolds, the present study aimed to synthesize a modified nHA/Cs/Gel biomimetic scaffold with improved features. Methods: Pure and copper (Cu)-substituted nHA was synthesized using the chemical precipitation method under controlled pH and temperature. Pure and Cu-substituted nHA/Cs/Gel scaffolds were fabricated by salt-leaching/freeze-drying method. Physicochemical characteristics of nanoparticles and scaffolds were explored using XRD, FTIR, FE-SEM/EDX, and ICP. Besides, scaffold mechanical strength, degradation, porosity, swelling, biomineralization, and cytocompatibility were assessed. Results: Pure and Cu-substituted nHA were synthesized and characterized with appropriate Cu substitution and improved physical properties. All scaffolds were highly porous (porosity >98%) and Cu incorporation reduced porosity from 99.555 ± 0.394% to 98.69 ± 0.80% while enlarged the pore size to more than100 µm. Cu-substitution improved the scaffold mechanical strength and the best result was observed in nHA.Cu5%/Cs/Gel scaffolds by the compressive strength 88.869 ± 19.574 MPa. Furthermore, 3% and 5% Cu-substituted nHA enhanced the scaffold structural stability and supported osteoblast spread, adhesion, survival, mineralization, and proliferation. Moreover, long-term and sustainable Cu release from scaffolds was observed within 28 days. Conclusion: Cu-substituted nHA/Cs/Gel scaffolds mimic the porous structure and mechanical strength of cancellous bone, along with prolonged degradation and Cu release, osteoblast attachment, viability, calcium deposition, and proliferation. Taken together, our results indicate the upgraded properties of nHA.Cu5%/Cs/Gel scaffolds for future applications in bone tissue engineering.

Effect of Hydroxyapatite: Fibroin Ratio on Hydroxyapatite/Fibroin/Chitosan Porous Scaffold Characteristics

2016 ◽  
Vol 705 ◽  
pp. 315-319
Author(s):  
Wassanai Wattanutchariya ◽  
Anirut Chaijaruwanich ◽  
Tarin Sukhachiradet
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
Chemical Precipitation ◽  
Precipitation Method ◽  
Porous Scaffolds ◽  
Fixed Amount ◽  
Replacement Technique ◽  
A New Technique

Autografting is a bone replacement technique used in orthopedic surgery. Bone tissue engineering is a new technique that offers promise, and could help alleviate this risk. Bioceramics, biopolymers or composite can be fabricated for artificial bone scaffold and used for bone regeneration. This study used three types of biomaterials – hydroxyapatite (HA), fibroin, and chitosan – to form porous scaffold. HA and fibroin were prepared from natural materials. HA was synthesized from mollusk shell by wet chemical precipitation method, while silk fibroin was extracted from silk worm’s cocoons. The HA and fibroin were mixed in a variety of ratios along with a fixed amount of chitosan before fabricating composite porous scaffolds by freeze-drying. The resulting scaffolds were evaluated for biodegradability, biocompatibility, porosity pore morphology and mechanical property. The fabricated scaffolds had an interconnected porous structure with a pore size of 200-400 μm and porosity in a range of 93-95%. The average degradation rate of the scaffold in lysozyme was between 7-17% at 7 days. A biocompatibility test showed that the scaffold was non-cytotoxic, making it a good candidate for future bone tissue engineering applications.

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