In Vitro Reactivity of Bioactive Glass Fibers

2006 ◽  
Vol 49 ◽  
pp. 246-251 ◽  
Author(s):  
Hanna Arstila ◽  
Mikko Tukiainen ◽  
Leena Hupa ◽  
Heimo O. Ylänen ◽  
Minna Kellomäki ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bioactive Glass ◽  
Glass Fibers ◽  
Time Intervals ◽  
Cross Sectional ◽  
Mechanical Durability ◽  
Stationary Conditions ◽  
Calcium Phosphate Precipitation

Implants with long lasting bioactivity and mechanical sustainability would be of interest in several novel clinical applications. By processing bioactive glass fibers and biodegradable polymers into 3D structures, bone formation ability of glasses and flexibility of polymers can be combined. In order to achieve desired physiological response, reactivity of bioactive glass fibers must be specified. Bundles of fibers within the range of bioactivity were soaked in the simulated body fluid at stationary conditions for several time intervals after which the cross-sectional surfaces of the fibers were studied with SEM-EDXA. The reaction layers and precipitations formed on the fiber surfaces suggest that the fibers react according to three mechanisms depending on the glass composition. Fibers with a high in vitro bioactivity showed the formation of distinct and thick silica –rich and calcium phosphate –rich layers already at one day’s immersion. Fibers of medium bioactivity did not show any clear silica –rich layer but a formation of calcium phosphate precipitations or layers at one day’s immersion. Slow glasses showed sporadic calcium phosphate precipitation only after the longest immersion times. The results indicate that the medium and slow glasses are interesting alternatives for applications where a long term mechanical durability suggested by their slow reactivity in combination with their osteoconductive tendency is desired.

Molecular Basis of Salivary Proline-rich Protein and Peptide Synthesis: Cell-free Translations and Processing of Human and Macaque Statherin mRNAs and Partial Amino Acid Sequence of their Signal Peptides

1987 ◽  
Vol 66 (2) ◽  
pp. 462-466 ◽  
Author(s):  
F.G. Oppenheim ◽  
D.I. Hay ◽  
D.J. Smith ◽  
G.D. Offner ◽  
R.F. Troxler
Keyword(s):  
Calcium Phosphate ◽  
Macaca Fascicularis ◽  
Electrophoretic Analysis ◽  
Proteolytic Processing ◽  
Oral Environment ◽  
Reticulocyte Lysate ◽  
Polyprotein Precursor ◽  
Phosphate Salts ◽  
Calcium Phosphate Precipitation

Acidic proline-rich phosphoproteins and phosphopeptides are abundant components of parotid and submandibular salivary secretions in man and in the subhuman primate, Macaca fascicularis. The major acidic proline-rich proteins and the proline-rich phosphopeptide, statherin, of man and macaques have been shown to be potent inhibitors of calcium phosphate precipitation and are thought to function in the oral environment by maintaining saliva supersaturated with respect to calcium phosphate salts. Little is known about the biosynthesis of these proline-rich phosphoproteins and peptides, and the aim of the present work was to determine the structural relationship between statherin precursors and native human and macaque statherin. RNA was isolated from human submandibular gland, and poly(A+) mRNA was selected by affinity chromatography on oligo(dT) cellulose and translated in a reticulocyte lysate. Electrophoretic analysis of the translation products revealed that this mRNA directed the synthesis of a large number of polypeptides with M,s ranging from 5000 to 70,000. Immunoprecipitates, prepared with an antiserum directed against human statherin, contained a single component with a Mr of 7800, approximately 2000 daltons larger than native statherin. Radiosequencing of the in vitro precursor of statherin in immunoprecipitates demonstrated the presence of a 19-residue signal peptide. These results suggest that statherin is derived from a unique structural gene, and does not result from proteolytic processing of a large polyprotein precursor.

Effect of Electrical Polarization on the Behavior of Bioactive Glass Containing MgO and B2O3 in SBF

2006 ◽  
Vol 309-311 ◽  
pp. 333-336
Author(s):  
Emiko Amaoka ◽  
Erik Vedel ◽  
Satoshi Nakamura ◽  
Yusuke Moriyoshi ◽  
Jukka I. Salonen ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Bioactive Glass ◽  
Body Fluid ◽  
Charged Surface ◽  
Depolarization Current ◽  
Electrical Polarization ◽  
Thermally Stimulated Depolarization Current ◽  
Thermally Stimulated Depolarization ◽  
Calcium Phosphate Precipitation

We investigated the electrical polarizability of MgO and B2O3 containing bioactive glass (MBG). The MBG material with good manufacturing properties but low bioactivity was electrically polarized at a high dc field. The electrical polarizability of MBG was evaluated by thermally stimulated depolarization current (TSDC) measurements and immersion in simulated body fluid (SBF). The early precipitation of calcium phosphate on the negatively charged surface of the treated MBG demonstrated the increased bioactivity of the material and confirmed its polarizability. It is suggested that the electrical interactions between the polarized MBG and ions in SBF promoted the formation of the calcium phosphate precipitation. Accordingly, the increased bioactivity of the MBG in SBF is suggested to demonstrate the conversion of MBG into electrovector ceramics by the polarization treatment.

The Primary Investigation on Biological Effect of Mesoporous Bioactive Glass In Vivo

2013 ◽  
Vol 750-752 ◽  
pp. 1651-1655
Author(s):  
Bai Yan Sui ◽  
Cheng Tie Wu ◽  
Jiao Sun
Keyword(s):  
Bioactive Glass ◽  
Biological Effect ◽  
Degradation Products ◽  
Long Term Effect ◽  
Liver And Kidney ◽  
Mesoporous Bioactive Glass

Mesoporous bioactive glass (MBG) has superior bioactivity and degradation than non-mesoporous bioactive glass (BG) in vitro. But the biological effect of MBG in vivo is still unknown. In this study, MBG powders with 20μm were implanted into the femoral condyles in SD rats. BG powders with 20μm were used as a control. The local degradation and osteogenesis were observed at 1 week and 4 weeks after implantation, and the systemic toxicity of the degradation products were also evaluated simultaneously. The results revealed MBG powders had the faster rate of degradation and better osteogenesis effect than BG powders at 4 weeks, although the most of material still remained in situ. Histopathological analyses indicated the degradation products did not have any damage to major organs such as liver and kidney. In conclusion, this preliminary study demonstrated that MBG powders have more excellent biological effect at 4 weeks than that of BG in vivo. However the long-term effect needs to be confirmed.

In vitro and in vivo bone formation potential of surface calcium phosphate-coated polycaprolactone and polycaprolactone/bioactive glass composite scaffolds

2016 ◽  
Vol 30 ◽  
pp. 319-333 ◽  
Author(s):  
Patrina S.P. Poh ◽  
Dietmar W. Hutmacher ◽  
Boris M. Holzapfel ◽  
Anu K. Solanki ◽  
Molly M. Stevens ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Bioactive Glass ◽  
Glass Composite ◽  
Composite Scaffolds ◽  
Formation Potential ◽  
In Vivo Bone Formation

scholarly journals Cariogenic and erosive potential of liquid oral pediatric medicines of long-term use for children: an in vitro analysis

2020 ◽  
Author(s):  
Ola B. Al-Batayneh ◽  
Ala'a B. Al-Bataina ◽  
Yousef S. Khader ◽  
Arwa I. Owais
Keyword(s):  
Dental Caries ◽  
Chronic Administration ◽  
Low Ph ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Vitro Analysis ◽  
High Sucrose ◽  
In Vitro Analysis

Abstract Background Liquid oral pediatric medicines (LOPM) contain sucrose and glucose and can have a low pH; their chronic administration may increase the risk for dental caries and erosion in children. The aim of this study was to determine sucrose and glucose concentrations, and pH of LOPMs of long-term use by children. Methods A cross sectional survey was conducted among 95/381 pharmacies to assess the most commonly prescribed LOPM by pharmacists in North Jordan, yielding 42 medicines which were analyzed for sucrose and glucose concentrations (mg/g) using HPLC-RID, and pH measurement using pH-meter CP-505. Data were analyzed using SPSS version 19.0. Differences were considered significant at P ≤ 0.05. Results The lowest and highest sucrose concentrations were for Ramlac® (0.9%) from the gastrointestinal medicines and Varolex® (79.5%) from the nutritional medicines, respectively. The lowest and highest glucose concentrations were for Adol® (0.0%) from the central nervous medicines and Pediavit-D® (81.1%) from the nutritional medicines, respectively. The pH ranged from 3.2 for Varolex® from the nutritional medicines to 10.0 for Zithromax® from the antibiotics medicines. Conclusions Some of the LOPMs studied had high sucrose or glucose concentrations (or both) and low pH which could increase their potential of causing dental caries and erosion when ingested frequently and chronically.

Stability of Ultrathin Amorphous Calcium Phosphate Artificial Enamel Sheets under Simulated Intraoral Environment Condition

2019 ◽  
Vol 829 ◽  
pp. 16-20
Author(s):  
Nobuhiro Kato ◽  
Mizuki Hirai ◽  
Ei Yamamoto ◽  
Shigeki Hontsu
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Dental Materials ◽  
Cross Sectional ◽  
Sem Image ◽  
Long Term Stability ◽  
Optical Images ◽  
Surface Morphologies ◽  
Environment Condition

Dental materials purely composed of calcium phosphate are ideal for the dental medicine. We have been developing ultrathin amorphous calcium phosphate (ACP) sheets for enamel repair. In this study, long term stability of the ultrathin ACP sheets applied on enamel surfaces which kept in a simulated intraoral condition for 21 days was evaluated. Surface morphologies of the ACP sheets were observed by optical images. A boundary condition between the ACP sheet and the enamel was evaluated by cross-sectional SEM image. A durability of the ACP sheet was evaluated by a brushing test conducted after 21 days incubation. As the result of these evaluations, it was confirmed that the ACP sheets were stable even after long-term incubation under the simulated intraoral environment condition.

scholarly journals Effect of Toothpaste Application Prior to Dental Bleaching on Whitening Effectiveness and Enamel Properties

2016 ◽  
Vol 41 (1) ◽  
pp. E29-E38 ◽  
Author(s):  
WF Vieira-Junior ◽  
DANL Lima ◽  
CPM Tabchoury ◽  
GMB Ambrosano ◽  
FHB Aguiar ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Repeated Measures ◽  
Potassium Nitrate ◽  
Positive Control ◽  
Negative Control ◽  
Cross Sectional ◽  
Dental Bleaching ◽  
Color Changes ◽  
Physical Surface

SUMMARY Objective The purpose of this study was to investigate the effects on the enamel properties and effectiveness of bleaching using 35% hydrogen peroxide (HP) when applying toothpastes with different active agents prior to dental bleaching. Methods Seventy enamel blocks (4 × 4 × 2 mm) were submitted to in vitro treatment protocols in a tooth-brushing machine (n=10): with distilled water and exposure to placebo gel (negative control [NC]) or HP bleaching (positive control [PC]); and brushing with differing toothpastes prior to HP bleaching, including potassium nitrate toothpaste (PN) containing NaF, conventional sodium monofluorophosphate toothpaste (FT), arginine-based toothpastes (PA and SAN), or a toothpaste containing bioactive glass (NM). Color changes were determined using the CIE L*a*b* system (ΔE, ΔL, Δa, and Δb), and a roughness (Ra) analysis was performed before and after treatments. Surface microhardness (SMH) and cross-sectional microhardness (CSMH) were analyzed after treatment. Data were analyzed with repeated measures ANOVA for Ra, one-way ANOVA (SMH, ΔE, ΔL, Δa, and Δb), split-plot ANOVA (CSMH), and Tukey post hoc test (α<0.05). The relationship between the physical surface properties and color properties was evaluated using a multivariate Canonical correlation analysis. Results Color changes were statistically similar in the bleached groups. After treatments, SMH and CSMH decreased in PC. SMH increased significantly in the toothpaste groups vs the negative and positive control (NM > PA = SAN > all other groups) or decreased HP effects (CSMH). Ra increased in all bleached groups, with the exception of NM, which did not differ from the NC. The variation in the color variables (ΔL, Δa, and Δb) explained 21% of the variation in the physical surface variables (Ra and SMH). Conclusion The application of toothpaste prior to dental bleaching did not interfere with the effectiveness of treatment. The bioactive glass based toothpaste protected the enamel against the deleterious effects of dental bleaching.

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