Osteoblastic Cell Differentiation on BMP-2 Pre-Adsorbed Octacalcium Phosphate and Hydroxyapatite

2007 ◽  
Vol 361-363 ◽  
pp. 1025-1028 ◽  
Author(s):  
Takashi Kumagai ◽  
Takahisa Anada ◽  
Yoshitomo Honda ◽  
Masamichi Takami ◽  
Ryutaro Kamijyo ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Room Temperature ◽  
Calcium Phosphates ◽  
Octacalcium Phosphate ◽  
Bone Morphogenetic Protein 2 ◽  
Osteoblastic Cell ◽  
Plastic Plate ◽  
Osteoblastic Cell Line ◽  
Ha Coating ◽  
Morphogenetic Protein

The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.

scholarly journals Probiotic Propionibacterium freudenreichii MJ2 Enhances Osteoblast Differentiation and Mineralization by Increasing the OPG/RANKL Ratio

2021 ◽  
Vol 9 (4) ◽  
pp. 673
Author(s):  
Jiah Yeom ◽  
Seongho Ma ◽  
Young-Hee Lim
Keyword(s):  
Signaling Pathway ◽  
Osteoblast Differentiation ◽  
Surface Proteins ◽  
Bone Morphogenetic Protein 2 ◽  
Ovariectomized Rats ◽  
Osteoblastic Cell ◽  
Enhancement Effect ◽  
Propionibacterium Freudenreichii ◽  
Alizarin Red ◽  
Osteoblastic Cell Line

Osteoblast differentiation is important for the development of bone and the maintenance of bone density. Propionibacterium freudenreichii is a probiotic with an anti-inflammatory property. The aim of this study was to investigate the enhancement effect of P. freudenreichii MJ2 (MJ2) isolated from raw milk on osteoblast differentiation, mineralization, and its signaling pathway. For in vitro and in vivo experiments, human fetal osteoblastic cell line hFOB 1.19 and an ovariectomized rat model were used, respectively. Expression levels of genes and proteins related to osteoblast differentiation and mineralization were measured by real-time polymerase chain reaction (qPCR) and Western blotting, respectively. Alizarin red S staining was performed to measure osteoblast mineralization. Heat-killed MJ2 (hkMJ2)-treated cells showed significantly increased osteoblast differentiation via an increase in the osteoprotegerin (OPG)/receptor activator of nuclear factor-κB ligand (RANKL) ratio and significantly increased osteoblast mineralization by stimulating the expression of bone morphogenetic protein 2 and runt-related transcription factor 2. Additionally, oral administration of live or heat-killed MJ2 to ovariectomized rats inhibited osteoporosis-induced bone loss. Specifically, surface proteins isolated from MJ2 promoted osteoblast differentiation and mineralization. In conclusion, MJ2 enhanced osteoblast differentiation and mineralization through the OPG/RANKL signaling pathway and the effective component of MJ2 might be its surface proteins.

scholarly journals Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cells

2020 ◽  
Author(s):  
Jaroslaw Cendrowski ◽  
Marta Kaczmarek ◽  
Katarzyna Kuzmicz-Kowalska ◽  
Michal Mazur ◽  
Kamil Jastrzebski ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Intracellular Transport ◽  
Regulatory System ◽  
Erythroid Differentiation ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Splicing Variants ◽  
Er Exit Sites ◽  
Autophagic Degradation ◽  
Cell Type Specific

ABSTRACTIntracellular transport undergoes remodeling upon cell differentiation, which involves cell type-specific regulators. Bone morphogenetic protein 2-inducible kinase (BMP2K) has been potentially implicated in endocytosis and cell differentiation but its molecular functions remained unknown. We discovered that its longer (L) and shorter (S) splicing variants regulate erythroid differentiation in a manner unexplainable by their involvement in AP-2 adaptor phosphorylation and endocytosis. However, both variants interacted with SEC16A whose silencing in K562 erythroid leukemia cells affected generation of COPII assemblies and induced autophagic degradation. Variant-specific depletion approach showed that BMP2K isoforms constitute a BMP2K-L/S regulatory system. Therein, L promotes while S restricts recruitment of SEC31A to SEC24B-containing COPII structures forming at SEC16A-positive ER exit sites. Finally, we found L to promote and S to restrict autophagic degradation. Hence, we propose that BMP2K-L favors SEC16A-dependent intracellular processes important for erythroid maturation, such as COPII trafficking and autophagy, in a manner inhibited by BMP2K-S.

scholarly journals Analysis of osteoblastic cell differentiation by synthetic octacalcium phosphate (OCP) as compared with commercially available β-TCP ceramic

2010 ◽  
Vol 56 (1) ◽  
pp. 2-8 ◽  
Author(s):  
Tadashi KAWAI ◽  
Takahisa ANADA ◽  
Yoshitomo HONDA ◽  
Shinji KAMAKURA ◽  
Aritsune MATSUI ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Octacalcium Phosphate ◽  
Osteoblastic Cell

Effect of Octacalcium Phosphate Ionic Dissolution Products on Osteoblastic Cell Differentiation

2007 ◽  
Vol 361-363 ◽  
pp. 31-34 ◽  
Author(s):  
Takahisa Anada ◽  
Akihiro Araseki ◽  
Shou Matsukawa ◽  
Tomokazu Yamasaki ◽  
Shinji Kamakura ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Conditioned Medium ◽  
Molecular Mechanisms ◽  
Octacalcium Phosphate ◽  
Osteoblastic Cell ◽  
Mouse Bone Marrow ◽  
Phosphate Ions ◽  
Proliferation And Differentiation

Our previous studies suggested that synthetic octacalcium phosphate (OCP) enhances bone regeneration more than hydroxyapatite (HA). However, the molecular mechanisms to induce osteogenic phenotype in osteoblast by OCP have not been identified. OCP tended to convert into an apatite structure in vivo and in vitro, and its process was accompanied by calcium consumption from the surrounding solution and the release of phosphate ions into the solution at a physiological condition. The present study was designed to investigate whether the dissolution of ionic products of OCP affects on proliferation and differentiation of mouse bone marrow stromal ST-2 cells in vitro. The number of cells treated with OCP-conditioned medium was slightly decreased in comparison to that of control at day 7. On the other hand, the level of alkaline phosphatase activity increased in OCP-conditioned medium. These results demonstrated that OCP is capable of inducing osteoblastic cell differentiation in ST-2 cells.

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