Silver-Doped Apatite as a Bioactive and an Antimicrobial Bone Material

Phase-pure nanostructured silver-doped hydroxyapatite (nAgHA) of various Ag contents was synthesised. These nAgHA were then evaluated in-vitro using human mesenchymal stem cells (hMSCs) and Escherichia coli (E. coli). Results revealed that hMSCs grew generally well on all nAgHA at all time points. In addition, fewer E. coli were seen attaching on the surface of all nAgHA. Hence, this work demonstrated that nAgHA offers considerable potential as a biomaterial.

Download Full-text

Biosynthesis of collagen I, II, RUNX2 and lubricin at different time points of chondrogenic differentiation in a 3D in vitro model of human mesenchymal stem cells derived from adipose tissue

Acta Histochemica ◽

10.1016/j.acthis.2014.09.008 ◽

2014 ◽

Vol 116 (8) ◽

pp. 1407-1417 ◽

Cited By ~ 46

Author(s):

Giuseppe Musumeci ◽

Ali Mobasheri ◽

Francesca Maria Trovato ◽

Marta Anna Szychlinska ◽

Adriana Carol Eleonora Graziano ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Chondrogenic Differentiation ◽

In Vitro Model ◽

Human Mesenchymal Stem Cells ◽

Time Points ◽

3D In Vitro Model ◽

Vitro Model

Download Full-text

Faculty Opinions recommendation of Expression of the p16INK4A gene is associated closely with senescence of human mesenchymal stem cells and is potentially silenced by DNA methylation during in vitro expansion.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1088258.541282 ◽

2007 ◽

Author(s):

Helen Gruber

Keyword(s):

Dna Methylation ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Human Mesenchymal Stem Cells ◽

P16ink4a Gene ◽

In Vitro Expansion

Download Full-text

Faculty Opinions recommendation of Proliferation as a requirement for in vitro chondrogenesis of human mesenchymal stem cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717960004.793463156 ◽

2012 ◽

Author(s):

David Lee ◽

Stephen Thorpe

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Human Mesenchymal Stem Cells

Download Full-text

Evaluation of Stemness Maintenance Properties of the Recombinant Human Laminin α2 LG1-3 Domains in Human Mesenchymal Stem Cells

Protein and Peptide Letters ◽

10.2174/0929866526666190617091155 ◽

2019 ◽

Vol 26 (10) ◽

pp. 785-791

Author(s):

Ji-Eun Kim ◽

Hye-Jin Seo ◽

SuJin Lee ◽

Jun-Hyeog Jang

Keyword(s):

Escherichia Coli ◽

Stem Cells ◽

Molecular Weight ◽

Mesenchymal Stem Cells ◽

Cell Adhesion ◽

Human Mesenchymal Stem Cells ◽

Adhesion Assay ◽

Proliferation Assay ◽

Undifferentiated State ◽

Laminin Α2

Background: Laminin, a member of the Extracellular Matrix (ECM), is a glycoprotein that is used as a factor that affects cell adhesion, proliferation, survival, and differentiation. Of these, five globular domains (LG domains) of the alpha chain play an important role in influencing the cell by binding to the integrin. Objective: This study aimed to evaluate the ability of globular domains 1-3 of laminin alpha2 (rhLAMA2LG1-3) in maintaining the pluripotency of human Mesenchymal Stem Cells (hMSCs), which are widely used in regenerative medicine. Methods: hMSCs were grown in the medium supplemented with rhLAMA2LG1-3, then the effect of the protein on hMSCs were confirmed through cell adhesion assay, proliferation assay and RTPCR. Results: rhLAMA2LG1-3 expressed in Escherichia coli has a molecular weight of 70 kDa, at 1 µg/ml concentration of rhLAMA2LG1-3, the attachment and proliferation of hMSCs were approximately 3.18-fold and 1.67-fold, respectively, more efficient than those of untreated controls. In addition, the undifferentiated state and degree of stemness of hMSCs were measured, on the basis of CD90 and CD105 levels. In the rhLAMA2LG1-3-treated hMSCs, the expression levels of CD90 and CD105 increased by 2.83-fold and 1.62-fold, respectively, compared to those in untreated controls. Conclusion: rhLAMA2LG1-3 can be potentially used in stem cell therapy to improve the viability and maintain the undifferentiated state of hMSCs.

Download Full-text

In Vitro Assessment of Hydroxyapatite Coating on the Surface of Additive Manufactured Ti6Al4V Scaffolds

Materials Science Forum ◽

10.4028/www.scientific.net/msf.879.2444 ◽

2016 ◽

Vol 879 ◽

pp. 2444-2449 ◽

Cited By ~ 1

Author(s):

Ekaterina Chudinova ◽

Maria Surmeneva ◽

Andrey Koptioug ◽

Irina V. Savintseva ◽

Irina Selezneva ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Human Mesenchymal Stem Cells ◽

Nanocrystalline Structure ◽

X Ray Diffraction ◽

Ha Coating ◽

Magnetron Sputter Deposition ◽

In Vitro Assessment ◽

Average Size

Custom orthopedic and dental implants may be fabricated by additive manufacturing (AM), for example using electron beam melting technology. This study is focused on the modification of the surface of Ti6Al4V alloy coin-like scaffolds fabricated via AM technology (EBM®) by radio frequency (RF) magnetron sputter deposition of hydroxyapatite (HA) coating. The scaffolds with HA coating were characterized by Scanning Electron microscopy, X-ray diffraction. HA coating showed a nanocrystalline structure with the crystallites of an average size of 32±9 nm. The ability of the surface to support adhesion and the proliferation of human mesenchymal stem cells was studied using biological short-term tests in vitro. In according to in vitro assessment, thin HA coating stimulated the attachment and proliferation of cells. Human mesenchymal stem cells cultured on the HA-coated scaffold also formed mineralized nodules.

Download Full-text

Chronic senescent human mesenchymal stem cells as possible contributor to the wound healing disorder after exposure to the alkylating agent sulfur mustard

Archives of Toxicology ◽

10.1007/s00204-020-02946-5 ◽

2021 ◽

Vol 95 (2) ◽

pp. 727-747

Author(s):

Simone Rothmiller ◽

Niklas Jäger ◽

Nicole Meier ◽

Thimo Meyer ◽

Adrian Neu ◽

...

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Mesenchymal Stem Cells ◽

Alkylating Agent ◽

Chronic Wounds ◽

Sulfur Mustard ◽

Morphological Changes ◽

Human Mesenchymal Stem Cells ◽

Age Related

AbstractWound healing is a complex process, and disturbance of even a single mechanism can result in chronic ulcers developing after exposure to the alkylating agent sulfur mustard (SM). A possible contributor may be SM-induced chronic senescent mesenchymal stem cells (MSCs), unable to fulfil their regenerative role, by persisting over long time periods and creating a proinflammatory microenvironment. Here we show that senescence induction in human bone marrow derived MSCs was time- and concentration-dependent, and chronic senescence could be verified 3 weeks after exposure to between 10 and 40 µM SM. Morphological changes, reduced clonogenic and migration potential, longer scratch closure times, differences in senescence, motility and DNA damage response associated genes as well as increased levels of proinflammatory cytokines were revealed. Selective removal of these cells by senolytic drugs, in which ABT-263 showed initial potential in vitro, opens the possibility for an innovative treatment strategy for chronic wounds, but also tumors and age-related diseases.

Download Full-text