Specific Detection of Staphylococcus Enterotoxin B in Milk Using Piezoresistive Cantilever Aptasensor

2015 ◽  
Vol 645-646 ◽  
pp. 1298-1302
Author(s):  
Rui Zhao ◽  
Yong Zheng Wen ◽  
Wei Ma ◽  
Jian Cheng Yang ◽  
Xiao Mei Yu
Keyword(s):  
High Sensitivity ◽  
Rapid Response ◽  
Experimental Results ◽  
Quick Response ◽  
Specific Detection ◽  
Detection Range ◽  
Coated Surface ◽  
Enterotoxin B ◽  
Food Commodities ◽  
Staphylococcus Enterotoxin B

In this paper, an aptasensor with high sensitivity and rapid response was developed for the detection of staphylococcus enterotoxin B (SEB) by using thiol-modified piezoresistive cantilever. Thiol-modified aptamers, acting as the functionalized sensing elements, were immobilized on the nanogold-coated surface of the sensing cantilever as agents for detecting SEB. By using the functionalized aptasensors, different concentrations of SEB were detected with a wide detection range of 6-100 ng/mL and a quick response in milk. The experimental results indicated that the cantilever-based aptasensors had sufficient sensitivity for the detection of SEB in real food commodities and might provide an economical platform for on-site detections of different toxicants with the advantages of portability, high sensitivity, and rapid response.

scholarly journals Validation of TaqMan-Based Assays for Specific Detection and Differentiation of Wild-Type and Neethling Vaccine Strains of LSDV

2021 ◽  
Vol 9 (6) ◽  
pp. 1234
Author(s):  
Dejan Vidanović ◽  
Bojana Tešović ◽  
Milanko Šekler ◽  
Zoran Debeljak ◽  
Nikola Vasković ◽  
...  
Keyword(s):  
Diagnostic Performance ◽  
High Sensitivity ◽  
Animal Disease ◽  
Wild Type ◽  
Notifiable Disease ◽  
Specific Detection ◽  
Lumpy Skin Disease ◽  
Systemic Reactions ◽  
Vaccination Campaigns ◽  
Significant Health

Lumpy skin disease (LSD) is an important animal disease with significant health and economic impacts. It is considered a notifiable disease by the OIE. Attenuated strains of LSDV have been successfully used as vaccines (LAV) but can also produce mild or systemic reactions. Vaccination campaigns using LAVs are therefore only viable if accompanying DIVA assays are available. Two DIVA qPCR assays able to distinguish Neethling-based LAVs and wild-type LSDV were developed. Upon validation, both assays were shown to have high sensitivity and specificity with a diagnostic performance comparable to other published DIVA assays. This confirmed their potential as reliable tools to confirm infection in animals during vaccination campaigns based on Neethling vaccine strains.

scholarly journals Ratiometric Fluorescent Biosensors for Glucose and Lactate Using an Oxygen-Sensing Membrane

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 208
Author(s):  
Hong Dinh Duong ◽  
Jong Il Rhee
Keyword(s):  
Limit Of Detection ◽  
Oxygen Sensing ◽  
High Sensitivity ◽  
Glucose Sensing ◽  
Oxidation Reactions ◽  
Lactate Oxidase ◽  
Lactate Oxidation ◽  
Detection Range ◽  
Quenching Effect ◽  
Sensing Membrane

In this study, ratiometric fluorescent glucose and lactate biosensors were developed using a ratiometric fluorescent oxygen-sensing membrane immobilized with glucose oxidase (GOD) or lactate oxidase (LOX). Herein, the ratiometric fluorescent oxygen-sensing membrane was fabricated with the ratio of two emission wavelengths of platinum meso-tetra (pentafluorophenyl) porphyrin (PtP) doped in polystyrene particles and coumarin 6 (C6) captured into silica particles. The operation mechanism of the sensing membranes was based on (i) the fluorescence quenching effect of the PtP dye by oxygen molecules, and (ii) the consumption of oxygen levels in the glucose or lactate oxidation reactions under the catalysis of GOD or LOX. The ratiometric fluorescent glucose-sensing membrane showed high sensitivity to glucose in the range of 0.1–2 mM, with a limit of detection (LOD) of 0.031 mM, whereas the ratiometric fluorescent lactate-sensing membrane showed the linear detection range of 0.1–0.8 mM, with an LOD of 0.06 mM. These sensing membranes also showed good selectivity, fast reversibility, and stability over long-term use. They were applied to detect glucose and lactate in artificial human serum, and they provided reliable measurement results.

A rapid and accurate method for screening T-2 toxin in food and feed using competitive AlphaLISA

2021 ◽  
Vol 368 (6) ◽  
Author(s):  
Liwen Zhang ◽  
Qingyu Lv ◽  
Yuling Zheng ◽  
Xuan Chen ◽  
Decong Kong ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Accurate Method ◽  
Detection Methods ◽  
Cereal Crops ◽  
Detection Range ◽  
Highly Sensitive ◽  
Food And Feed ◽  
Good Repeatability ◽  
Feed Samples ◽  
Better Than

ABSTRACT T-2 is a common mycotoxin contaminating cereal crops. Chronic consumption of food contaminated with T-2 toxin can lead to death, so simple and accurate detection methods in food and feed are necessary. In this paper, we establish a highly sensitive and accurate method for detecting T-2 toxin using AlphaLISA. The system consists of acceptor beads labeled with T-2-bovine serum albumin (BSA), streptavidin-labeled donor beads and biotinylated T-2 antibodies. T-2 in the sample matrix competes with T-2-BSA for antibodies. Adding biotinylated antibodies to the test well followed by T-2 and T-2-BSA acceptor beads yielded a detection range of 0.03–500 ng/mL. The half-maximal inhibitory concentration was 2.28 ng/mL and the coefficient of variation was <10%. In addition, this method had no cross-reaction with other related mycotoxins. This optimized method for extracting T-2 from food and feed samples achieved a recovery rate of approximately 90% in T-2 concentrations as low as 1 ng/mL, better than the performance of a commercial ELISA kit. This competitive AlphaLISA method offers high sensitivity, good specificity, good repeatability and simple operation for detecting T-2 toxin in food and feed.

scholarly journals High sensitivity and rapid response ultraviolet photodetector of a tetragonal CsPbCl3 perovskite single crystal

2020 ◽  
Vol 10 (6) ◽  
pp. 1374 ◽  
Author(s):  
Ziyue Rao ◽  
Wenyi Liang ◽  
Haiming Huang ◽  
Jun Ge ◽  
Weiliang Wang ◽  
...  
Keyword(s):  
Single Crystal ◽  
High Sensitivity ◽  
Rapid Response ◽  
Ultraviolet Photodetector

scholarly journals Portable smartphone-integrated paper sensors for fluorescence detection of As(III) in groundwater

2020 ◽  
Vol 7 (12) ◽  
pp. 201500
Author(s):  
Sha Liu ◽  
Yong Li ◽  
Chao Yang ◽  
Liqiang Lu ◽  
Yulun Nie ◽  
...  
Keyword(s):  
Environmental Problem ◽  
Visual Detection ◽  
High Sensitivity ◽  
Integration System ◽  
Detection Range ◽  
Sensitivity And Selectivity ◽  
Toxic Metalloid ◽  
Interfering Ions ◽  
Cu Nanoclusters

Arsenic contamination in groundwater is a supreme environmental problem, and levels of this toxic metalloid must be strictly monitored by a portable, sensitive and selective analytical device. Herein, a new system of smartphone-integrated paper sensors with Cu nanoclusters was established for the effective detection of As(III) in groundwater. For the integration system, the fluorescence emissive peak of Cu nanoclusters at 600 nm decreased gradually with increasing As(III) addition. Meanwhile, the fluorescence colour also changed from orange to colourless, and the detection limit was determined as 2.93 nM (0.22 ppb) in a wide detection range. The interfering ions also cannot influence the detection selectivity of As(III). Furthermore, the portable paper sensors based on Cu nanoclusters were fabricated for visual detection of As(III) in groundwater. The quantitative determination of As(III) in natural groundwater has also been accomplished with the aid of a common smartphone. Our work has provided a portable and on-site detection technique toward As(III) in groundwater with high sensitivity and selectivity.

scholarly journals Wearable Pressure Sensors Based on Carbonized Graphene Coated Waste Paper Aerogel

2021 ◽  
Author(s):  
Ang Li ◽  
Ce Cui ◽  
Weijie Wang ◽  
Yue Zhang ◽  
Jianyu Zhai ◽  
...  
Keyword(s):  
3D Structure ◽  
Pressure Sensors ◽  
High Sensitivity ◽  
Waste Paper ◽  
Low Density ◽  
Detection Range ◽  
Simple Strategy ◽  
Simple Filtration ◽  
Piezoresistive Pressure Sensors ◽  
Main Component

Abstract Graphene is complexed with cellulose fibers to construct 3D aerogels, which is generally considered to be an environmentally friendly and simple strategy to achieve wide sensing, high sensitivity and low detection of wearable piezoresistive pressure sensors. Here, graphene is incorporated into waste paper fibers with cellulose as the main component to prepare graphene coated waste paper aerogel (GWA) using a simple “filtration-oven drying” method under atmospheric pressure. The GWA was further annealed to obtain the carbonized graphene coated waste paper aerogel (C-GWA) to achieve low density and excellent resilience. The result shows that the C-GWA has a rough outer surface due to the 3D structure formed by interpenetrated fibers and the carbon skeleton with wrinkles. The sensor based on GCA shows low density (25mg/cm3), a wide detection range of 0-132 kPa, an ultra-low detection limit of 2.5 Pa (a green bean, ≈ 53.4 mg), and a high sensitivity of 31.6 kPa− 1. In addition, the sensor based on C-GWA with the excellent performance can be used to detect human motions including the pulse of the human body, cheek blowing and bending of human joints. The result indicates that the sensor based on C-GWA shows great potential for wearable electronic products.

Trifluoromethyl-substituted cyclometalated iridiumIII emitters with high photostability for continuous oxygen sensing

2015 ◽  
Vol 3 (31) ◽  
pp. 8010-8017 ◽  
Author(s):  
Chun Liu ◽  
Xin Lv ◽  
Yang Xing ◽  
Jieshan Qiu
Keyword(s):  
Oxygen Sensing ◽  
High Sensitivity ◽  
Quantum Yields ◽  
Quick Response ◽  
Oxidation Potentials ◽  
Enhanced Photostability ◽  
Oxygen Concentrations

New trifluoromethyl-substituted cyclometalated Ir(iii) complexes exhibit high photoluminescence quantum yields, increased oxidation potentials and enhanced photostability. The oxygen sensing films immobilized with Ir(iii) complexes demonstrate quick response and high sensitivity upon changes of oxygen concentrations.

An Electrochemically Reduced Copper/Reduced Graphene Oxide Film Modified Electrode for Sensitive Non-Enzymatic Glucose Detection in Human Serum

2021 ◽  
Author(s):  
Sopit Phetsang ◽  
Pinit Kidkhunthod ◽  
Narong Chanlek ◽  
Jaroon Jakmunee ◽  
Pitchaya Mungkornasawakul ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Human Serum ◽  
Reduced Graphene Oxide ◽  
Modified Electrode ◽  
Electrocatalytic Activity ◽  
Glucose Sensor ◽  
Electrochemical Sensors ◽  
High Sensitivity ◽  
Detection Range ◽  
Reduced Graphene

Abstract Numerous studies suggest that modification with functional nanomaterials can enhance the electrode electrocatalytic activity, sensitivity, and selectivity of the electrochemical sensors. Here, a highly sensitive and cost-effective disposable non-enzymatic glucose sensor based on copper(II)/reduced graphene oxide modified screen-printed carbon electrode is demonstrated. Facile fabrication of the developed sensing electrodes is carried out by the adsorption of copper(II) onto graphene oxide modified electrode, then following the electrochemical reduction. The proposed sensor illustrates good electrocatalytic activity toward glucose oxidation with a wide linear detection range from 0.10 mM to 12.5 mM, low detection limit of 65 µM, and high sensitivity of 172 µA mM− 1 cm− 2 along with satisfactory anti-interference ability, reproducibility, stability, and the acceptable recoveries for the detection of glucose in a human serum sample (95.6–106.4%). The copper(II)/reduced graphene oxide based sensor with the superior performances is a great potential for the quantitation of glucose in real samples.

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