Antioxidant Activity of Sulfated Polysaccharide Extract from Green Seaweed (Caulerpa lentillifera) Makassar, Indonesia

2020 ◽  
Vol 840 ◽  
pp. 214-220
Author(s):  
Ilmi Fadhilah Rizki ◽  
Ellya Sinurat ◽  
Sofa Fajriah ◽  
Endang Saefudin
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Biological Activities ◽  
Sulfated Polysaccharide ◽  
Total Carbohydrate ◽  
Caulerpa Lentillifera ◽  
Green Seaweed ◽  
Ft Ir ◽  
Crude Polysaccharide ◽  
Ft Ir Spectroscopy

Caulerpa lentillifera belong to Caulerpa genus which is commonly found in tropical and subtropical water. The biggest constituent of seaweed is polysaccharide that has some biological activities as a potential medicine. Therefore, this research aimed to extract and evaluate the antioxidant activity from Caulerpa lentillifera polysaccharide. The extraction was carried out by using water extraction. First, the sample was added with ethanol and soaked overnight at room temperature. On the following day, the sample was added with aquades and put in a water bath at 75°C for three hours. After that, ethanol was added to precipitate the extract. The crude polysaccharide extract percent yield obtained 4.16 %. The crude extract purified by using a column with DEAE-Sepharose with percent yield obtained 14.8 %. Both crude and pure extracts were characterized by analyzing the total carbohydrate and sulfate by using spectrophotometer, functional group by using FT-IR spectroscopy and sugar component by using HPLC. Antioxidant activity was analyzed by using the FRAP method for both crude and pure extract. Moreover, the polysaccharide crude extract gives higher antioxidant activity than the purified extract.

scholarly journals Characterization and analysis of the antidiabetic activities of sulphated polysaccharide extract from Caulerpa lentillifera

Pharmacia ◽  
2021 ◽  
Vol 68 (4) ◽  
pp. 869-875
Author(s):  
Sofa Fajriah ◽  
Ilmi Fadhilah Rizki ◽  
Ellya Sinurat
Keyword(s):  
Crude Extract ◽  
Minor Component ◽  
Antidiabetic Activity ◽  
Sulfated Polysaccharides ◽  
Antidiabetic Medication ◽  
Total Carbohydrate ◽  
Caulerpa Lentillifera ◽  
Green Seaweed ◽  
A Minor ◽  
Initial Process

Caulerpa lentillifera is a type of green seaweed that is cultivated in tropical and subtropical areas. The objectives of this study were to determine the characteristics of the sulfated polysaccharides from C. lentillifera and evaluate its antidiabetic activity. In the initial process of this study, samples were macerated with ethanol (1:10). Then, the maceration residue was extracted with an accumulator at 75 °C for three hours. The crude extract yield was 4.16% based on weight seaweed. Ion chromatography purification with DEAE-Sepharose resin provided a yield of 14.8% of crude extract. The monomer analysis of C. lentillifera from the crude extract and purified extract revealed that galactose monomers were dominant and glucose was a minor component. The total carbohydrate and sulfate contents of purified C. lentillifera were higher than those of crude C. lentillifera. Bioactivity tests revealed that purified polysaccharides had higher antidiabetic activity against α-glucosidase enzyme than crude ones with IC50 values of 134.81± 2.0 µg/mL. Purified sulfated polysaccharides of C. lentillifera could potentially be used as an antidiabetic medication.

scholarly journals Betalains in Edible Fruits of Three Cactaceae Taxa—Epiphyllum, Hylocereus, and Opuntia—Their LC-MS/MS and FTIR Identification and Biological Activities Evaluation

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2669
Author(s):  
Michaela Barkociová ◽  
Jaroslav Tóth ◽  
Katarzyna Sutor ◽  
Natalia Drobnicka ◽  
Slawomir Wybraniec ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Food Industry ◽  
Biological Activities ◽  
Ornamental Plants ◽  
Reducing Power ◽  
Activity Assay ◽  
Natural Pigment ◽  
E Coli ◽  
Ft Ir

Epiphyllum, Hylocereus, and Opuntia plants belong to the Cactaceae family. They are mostly known as ornamental plants but also for their edible fruits, which can potentially be sources of betalains, such as betanin, a natural pigment used in the food industry, e.g., under the European label code E 162. The aim of this work was the identification of betalains (using LC-MS/MS), evaluation of total betalain content (spectrophotometrically), analysis of functional groups (using FT-IR), evaluation of antioxidant activity (using DPPH, ABTS, FRAP, DCFH-DA, and reducing power methods) and evaluation of antimicrobial activity (S. aureus, E. coli, and C. albicans) in fruits of Epiphyllum, Hylocereus, and Opuntia taxa. A total of 20 betalains were identified in the studied Cactaceae fruits. The Epiphyllum pink hybrid had the highest values of total betalains amongst all samples. The highest antioxidant activity was observed in the Epiphyllum pink hybrid, in Opuntia zacuapanensis and O. humifusa fruits. The antimicrobial activity assay showed that cacti fruits were not able to effectively inhibit the growth of E. coli, S. aureus, or C. albicans. Our results prove that these fruits are good sources of natural pigments—betalains. They do not contain toxic compounds in significant amounts and they exhibit antioxidant activity.

scholarly journals Polyphenol Enriched Extract of Pomegranate Peel; A Novel Precursor for the Biosynthesis of Zinc Oxide Nanoparticles and Application in Sunscreens

2020 ◽  
Vol 27 (1) ◽  
pp. 102-110
Author(s):  
Maryam Kokabi ◽  
Samad Nejad Ebrahimi
Keyword(s):  
Antioxidant Activity ◽  
Zinc Oxide ◽  
Ir Spectroscopy ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Pomegranate Peel ◽  
Ft Ir ◽  
Oil Emulsions ◽  
Ft Ir Spectroscopy

Background: Green synthesized nanoparticles (NPs) from agricultural wastes is an area of great interest due to it is eco-friendly and profitable. Zinc oxide is an inorganic UV-filter commonly used as UV-blocker in a different industry. Methods: Zinc oxide nanoparticles (ZnO NPs) were successfully biosynthesized using Zn(NO3)2 as a substrate by polyphenol enriched fraction (PEF) of pomegranate peel. The biological activity of ZnO NPs was evaluated using MBC and MIC tests for antibacterial and DPPH assay for antioxidant potential. Sunscreen potential of NPs was determined after applying them in water-in-oil emulsions. Results: UV-Vis and FT-IR spectroscopy techniques confirmed the formation of ZnO NPs. FE-SEM characterized the morphology and purity of the biosynthesized NPs with EDAX and XRD data. The average crystalline size of ZnO NPs was found to be 22 nm. FT-IR spectroscopy revealed the role of phenolic compounds in the formation and stability of ZnO NPs. The antibacterial activity of PEF and its biosynthesized ZnO was evaluated against Staphylococcus aureus and Escherichia coli. The prepared NPs showed a higher antibacterial effect than the commercial ZnO NPs. Interestingly, the antioxidant activity was also detected for obtained NPs. The PEF powder also exhibited higher antibacterial and antioxidant activity than the standards. Furthermore, the in vitro sun protection factors were estimated after applying NPs in water-in-oil emulsions. Conclusion: This study highlighted the possibility of using PEF of pomegranate peel for the biosynthesis of ZnO NPs as well as applying its NPs in sunscreens to achieve a safe alternative to harmful chemical UV-filters commonly used in cosmetics.

Spectroscopic Evaluation of the Effect of a Red Microalgal Polysaccharide on Herpes-Infected Vero Cells

2003 ◽  
Vol 57 (4) ◽  
pp. 390-395 ◽  
Author(s):  
Mahmoud Huleihel ◽  
Marina Talyshinsky ◽  
Yelena Souprun ◽  
Vitaly Erukhimovitch
Keyword(s):  
Ir Spectroscopy ◽  
Sugar Content ◽  
Antiviral Agent ◽  
Vero Cells ◽  
Sulfated Polysaccharide ◽  
Sugar Accumulation ◽  
Infected Cells ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

The sulfated polysaccharide obtained from a species of red microalga has proved to be a potent antiviral agent against various members of the herpes family. In the present study, we used microscopic Fourier transform infrared spectroscopy (FT-IR) to investigate differences between normal cells, those infected with herpes viruses, and infected cells treated with red microalgal polysaccharide. FT-IR enables the characterization of cell or tissue pathology based on characteristic molecular vibrational spectra of the cells. The advantage of microscopic FT-IR spectroscopy over conventional FT-IR spectroscopy is that it facilitates inspection of restricted regions of cell cultures or tissue. Our results showed significant spectral differences at early stages of infection between infected and noninfected cells, and between infected cells treated with the polysaccharide and those not treated. In infected cells, there was an impressive decrease in sugar content and a considerable increase in phosphate levels in conjunction with the infection progress. Our results also proved that sugars penetrated and accumulated inside cells treated with the red microalgal polysaccharide. These could have been sugar fragments of low molecular weight present in the polysaccharide solution, despite purification by dialysis. Such sugar accumulation might be responsible for a breakdown in the internal steps of the viral replication cycle.

scholarly journals Ulvan as A New Trend in Agriculture, Food Processing and Medicine

2020 ◽  
pp. 47-54
Author(s):  
Hadear Hanie Amin
Keyword(s):  
Food Processing ◽  
Gel Permeation Chromatography ◽  
Sulfated Polysaccharide ◽  
Hot Water ◽  
Human Pathogen ◽  
Hot Water Extraction ◽  
Gel Permeation ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

In this work the researcher is looking for natural, new, safe, cheap and available compounds that can be used as antimicrobial and antioxidant in the medical and agricultural fields. A sulfated polysaccharide, Ulvan was extracted from Ulva lactuca and purified by cold or hot water-extraction and ethanol-precipitation with yield = 5:10% (w-w). Its appearance and touch like gel. Characterization of active groups of Ulvan was achieved by FT-IR spectroscopy, its content of sulfate = 3.998% (w-w) was determined by Ion Chromatography (IC), its molecular weight = 532.221 Kilo Daltons was determined by Gel permeation chromatography, its thermal stability was evaluated by DSC-TGA, its viscosity = 18 was determined by viscometer apparatus and its antimicrobial activity was studied against human, plant, animal, fish and poultry pathogens. Ulvan showed antioxidant activity with IC50 = 3.04 mg/ml. Its biological activity as an antioxidant and antimicrobial may be a good indication for the use of Ulvan against various pathogens as a preservative in food processing and cosmetics and as antioxidant and antimicrobial in medicine against human pathogen.

scholarly journals Chemical and Spectral Characterization of The Ozonation Products of κ-Carrageenan

2018 ◽  
Vol 156 ◽  
pp. 05006 ◽  
Author(s):  
Aji Prasetyaningrum ◽  
Bakti Jos ◽  
Yudhy Dharmawan ◽  
Ratih V. Octaviani ◽  
Ratnawati Ratnawati
Keyword(s):  
Chemical Structure ◽  
Biological Activities ◽  
Uv Spectra ◽  
Ozone Treatment ◽  
Ft Ir ◽  
Forced Air ◽  
Absorbance Peak ◽  
Ozonation Process ◽  
Ft Ir Spectroscopy

Kappa (κ-) carrageenan oligomers are known to have several biological activities. Recent progress in the development of modified κ-carrageenan has resulted low molecular of κ-carrageenan. Ozone is a powerful oxidant and considered for depolymerization of κ-carrageenan. However, few studies have investigated the changes in κ-carrageenan properties associated with ozone treatment. This study would investigate on the changes in chemical structure after ozonation process. The experiments were carried out in a glass reactor equipped with an ozone bubble diffuser. Ozone with concentration of 80 ± 2 was bubbled into the solution. The ozone treatment was conducted at different times, i.e., 0 (control), 5, 10, 15, and 20 minutes. The experiments were conducted at pH 7 and constant stirring speed (200 rpm). Ozone-treated κ-carrageenan was dried at 60 ºC for 24 h in a forced air oven. The chemical and spectral analyses of κ-carrageenan after ozonation process were carried out using UV-Vis and FT-IR spectroscopy. These changes are seen in the UV spectra as a high intensity of absorbance peak at 290 nm. It is shows that ozonation of κ-carrageenan leads to some chemical changes such as the formation of carbonyl, carboxyl or double bonds.The FT-IR spectra reveals that the chemical structure of degraded κ-carrageenan, in term of sulfate content, is only slightly affected by the ozone treatment.

scholarly journals Chemical Properties, Fatty-Acid Composition, and Antioxidant Activity of Goji Berry (Lycium barbarum L. and Lycium chinense Mill.) Fruits

Antioxidants ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 60 ◽  
Author(s):  
Prodromos Skenderidis ◽  
Dimitrios Lampakis ◽  
Ioannis Giavasis ◽  
Stefanos Leontopoulos ◽  
Konstantinos Petrotos ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Fatty Acid ◽  
Radical Scavenging ◽  
Attenuated Total Reflection ◽  
Lycium Chinense ◽  
Total Carbohydrate ◽  
Lycium Barbarum ◽  
Goji Berry ◽  
Ft Ir

In this study, the content composition and antioxidant activity of goji berry fruits from two species (Lycium barbarum and Lycium chinense) were assessed. The total carbohydrate and phenolic contents were evaluated using attenuated total reflection Fourier-transform infrared (ATR-FT-IR) spectroscopy, while the antioxidant activity of fruits was examined with two in vitro methods, which are based on the scavenging activity of the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2’-azino-bis(3-ethyl-benzthiazoline-sulfonic acid) (ABTS•+) free radicals. The fatty-acid profile was determined using gas chromatography coupled with mass spectrometry (GC-MS). The results of this study indicate that the fruits of L. barbarum present higher concentrations in carbohydrates and phenolics than L. chinense Mill. fruits. Furthermore, the antioxidant activity based on the half maximal inhibitory concentration (IC50) measurements of DPPH• and ABTS•+ free-radical scavenging was higher in L. barbarum than L. chinense Mill. Also, the GCMS analysis confirms the high levels of linoleic, palmitic, and oleic acids contained in the fruits of both species. Finally, the results of this study clearly show that the concentration of bioactive and antioxidant molecules is higher in L. barbarum than in L. chinense fruits, which was also confirmed by ATR-FT-IR measurements.

scholarly journals Study of phenolic profile and antioxidant activity in selected Moravian wines during winemaking process by FT-IR spectroscopy

2015 ◽  
Vol 52 (10) ◽  
pp. 6405-6414 ◽  
Author(s):  
Jana Preserova ◽  
Vaclav Ranc ◽  
David Milde ◽  
Vladimira Kubistova ◽  
Jan Stavek
Keyword(s):  
Antioxidant Activity ◽  
Ir Spectroscopy ◽  
Phenolic Profile ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

scholarly journals Unexpected ring-opening of 2,3-dihydropyridines

2021 ◽  
Author(s):  
Michael-Hannes Hoffelner ◽  
Werner Seebacher ◽  
Marcel Kaiser ◽  
Pascal Mäser ◽  
Eva-Maria Pferschy-Wenzig ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Ir Spectroscopy ◽  
Ring Opening ◽  
Biological Activities ◽  
Open Chain ◽  
X Ray ◽  
Separation Of Isomers ◽  
Ft Ir ◽  
New Compounds ◽  
Ft Ir Spectroscopy

AbstractThe reaction of 2,3-dihydropyridines with sulfonyl halides surprisingly yielded open chain dienes with sulfonylimine structure. The products were specific out of several possible isomers and, therefore, a separation of isomers was not necessary. All new compounds were characterized using FT-IR spectroscopy, HRMS, and NMR spectroscopy. A bicyclic by-product from the reaction of a 2,3-dihydropyridine with mesyl chloride was isolated and its structure elucidated using a single X-ray crystal analysis. Some biological activities, like antimicrobial and cytotoxic properties were investigated. Graphic abstract

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