Evaluating H2O2 in Living Bacteria by Ratiometric Fluorescent Probe

2022 ◽  
Vol 905 ◽  
pp. 210-217
Author(s):  
Qian Qian Chen
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Gram Negative Bacteria ◽  
Signal Molecule ◽  
Gram Negative ◽  
Key Factor ◽  
Live Bacteria ◽  
First Time ◽  
Living Bacteria ◽  
Ratiometric Fluorescent Probe

Hydrogen peroxide (H2O2) is a significant signal molecule in physiological and pathological processes. Levels of H2O2 in bacteria are proved to be a key factor in immune response. To sum up, detection of H2O2 levels in living bacteria is remarkable for further study of its physiological and pathological effects. Herein, we propose a novel ratiometric fluorescent probe (Nahp) to detect H2O2 in living cells and bacteria. In addition, based on boronate, Nahp has satisfactory selectivity and sensitivity toward H2O2 (LOD = 0.158 μM). Furthermore, with excellent detection performance to H2O2, Nahp is successfully used for fluorescent bioimaging of H2O2 and measuring H2O2 accumulation in bacteria. Most importantly, the probe was also used to image H2O2 in three Gram-negative bacteria, clearly revealing for the first time significant differences in H2O2 expression levels in live bacteria.

An ESIPT-based ratiometric fluorescent probe for the imaging of nitroxyl in living cells

2015 ◽  
Vol 7 (9) ◽  
pp. 3883-3887 ◽  
Author(s):  
Hao-Ming Lv ◽  
Yi Chen Yi Chen ◽  
Jian Lei ◽  
Chak-Tong Au ◽  
Shuang-Feng Yin
Keyword(s):  
Excited State ◽  
Proton Transfer ◽  
Fluorescent Probe ◽  
Intramolecular Proton Transfer ◽  
Living Cells ◽  
Transfer Mechanism ◽  
First Time ◽  
Ratiometric Fluorescent Probe

A novel fluorescent probe based on the excited state intramolecular proton transfer mechanism for the detection of nitroxyl was developed for the first time.

scholarly journals The antimicrobial potential of cannabidiol

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Mark A. T. Blaskovich ◽  
Angela M. Kavanagh ◽  
Alysha G. Elliott ◽  
Bing Zhang ◽  
Soumya Ramu ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Modern Medicine ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
New Class ◽  
Clostridioides Difficile ◽  
Excellent Activity ◽  
Induce Resistance ◽  
First Time

AbstractAntimicrobial resistance threatens the viability of modern medicine, which is largely dependent on the successful prevention and treatment of bacterial infections. Unfortunately, there are few new therapeutics in the clinical pipeline, particularly for Gram-negative bacteria. We now present a detailed evaluation of the antimicrobial activity of cannabidiol, the main non-psychoactive component of cannabis. We confirm previous reports of Gram-positive activity and expand the breadth of pathogens tested, including highly resistant Staphylococcus aureus, Streptococcus pneumoniae, and Clostridioides difficile. Our results demonstrate that cannabidiol has excellent activity against biofilms, little propensity to induce resistance, and topical in vivo efficacy. Multiple mode-of-action studies point to membrane disruption as cannabidiol’s primary mechanism. More importantly, we now report for the first time that cannabidiol can selectively kill a subset of Gram-negative bacteria that includes the ‘urgent threat’ pathogen Neisseria gonorrhoeae. Structure-activity relationship studies demonstrate the potential to advance cannabidiol analogs as a much-needed new class of antibiotics.

Ultrastructural studies of Chlamydia psittaci 6BC variant strains. I. Ultrastructure of the surface layers of egg-passaged 6BC strain

1973 ◽  
Vol 19 (8) ◽  
pp. 887-894
Author(s):  
Linda Poffenroth ◽  
J. W. Costerton ◽  
Nonna Kordová ◽  
John C. Wilt
Keyword(s):  
Chick Embryo ◽  
Microscopic Examination ◽  
Electron Microscopic Examination ◽  
Chlamydia Psittaci ◽  
Gram Negative Bacteria ◽  
Surface Layers ◽  
Electron Microscopic ◽  
Gram Negative ◽  
Ultrastructural Studies ◽  
First Time

Electron microscopic examination of a semipurified Chlamydia psittaci 6BC strain attenuated in chick embryo yolk sac revealed for the first time two morphologically distinct small elementary bodies which differ both in the ultrastructure of their surface layers and in their buoyant densities in sucrose gradients. Also, the morphology of the surface layers of the larger reticulate forms in cell-free systems is described for the first time. Many points of difference between the surface envelopes and internal structure of chlamydial particles and those of Gram-negative bacteria are discussed.

A novel nopinone-based colorimetric and ratiometric fluorescent probe for detection of bisulfite and its application in food and living cells

2019 ◽  
Vol 171 ◽  
pp. 107702 ◽  
Author(s):  
Qian Jiang ◽  
Zhonglong Wang ◽  
Mingxin Li ◽  
Jie Song ◽  
Yiqin Yang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Ratiometric Fluorescent Probe

scholarly journals Shadowing the Actions of a Predator: Backlit Fluorescent Microscopy Reveals Synchronous Nonbinary Septation of Predatory Bdellovibrio inside Prey and Exit through Discrete Bdelloplast Pores

2010 ◽  
Vol 192 (24) ◽  
pp. 6329-6335 ◽  
Author(s):  
A. K. Fenton ◽  
M. Kanna ◽  
R. D. Woods ◽  
S.-I. Aizawa ◽  
R. E. Sockett
Keyword(s):  
Cell Division ◽  
Outer Membrane ◽  
Fluorescent Microscopy ◽  
Gram Negative Bacteria ◽  
Bacterial Cell Division ◽  
Prey Cell ◽  
Gram Negative ◽  
A Genome ◽  
Predatory Bacteria ◽  
First Time

ABSTRACT The Bdellovibrio are miniature “living antibiotic” predatory bacteria which invade, reseal, and digest other larger Gram-negative bacteria, including pathogens. Nutrients for the replication of Bdellovibrio bacteria come entirely from the digestion of the single invaded bacterium, now called a bdelloplast, which is bound by the original prey outer membrane. Bdellovibrio bacteria are efficient digesters of prey cells, yielding on average 4 to 6 progeny from digestion of a single prey cell of a genome size similar to that of the Bdellovibrio cell itself. The developmental intrabacterial cycle of Bdellovibrio is largely unknown and has never been visualized “live.” Using the latest motorized xy stage with a very defined z-axis control and engineered periplasmically fluorescent prey allows, for the first time, accurate return and visualization without prey bleaching of developing Bdellovibrio cells using solely the inner resources of a prey cell over several hours. We show that Bdellovibrio bacteria do not follow the familiar pattern of bacterial cell division by binary fission. Instead, they septate synchronously to produce both odd and even numbers of progeny, even when two separate Bdellovibrio cells have invaded and develop within a single prey bacterium, producing two different amounts of progeny. Evolution of this novel septation pattern, allowing odd progeny yields, allows optimal use of the finite prey cell resources to produce maximal replicated, predatory bacteria. When replication is complete, Bdellovibrio cells exit the exhausted prey and are seen leaving via discrete pores rather than by breakdown of the entire outer membrane of the prey.

A lysosome-targeted ratiometric fluorescent probe for detection of hypochlorous acid in living cells

2017 ◽  
Vol 247 ◽  
pp. 736-741 ◽  
Author(s):  
Qiong Yuan ◽  
Zhi-Min Zhao ◽  
Yan-Ru Zhang ◽  
Le Su ◽  
Jun-Ying Miao ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Ratiometric Fluorescent Probe

A ratiometric fluorescent probe for the detection of endogenous hydroxyl radicals in living cells

Talanta ◽  
2019 ◽  
Vol 196 ◽  
pp. 317-324 ◽  
Author(s):  
Biliu Wu ◽  
Jiajun Yang ◽  
Jianjian Zhang ◽  
Zheng Li ◽  
Hua Li ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hydroxyl Radicals ◽  
Living Cells ◽  
Ratiometric Fluorescent Probe

A ratiometric fluorescence sensor for HOCl based on a FRET platform and application in living cells

RSC Advances ◽  
2016 ◽  
Vol 6 (21) ◽  
pp. 17059-17063 ◽  
Author(s):  
Yan-Ru Zhang ◽  
Zhi-Min Zhao ◽  
Le Su ◽  
Jun-Ying Miao ◽  
Bao-Xiang Zhao
Keyword(s):  
Fluorescent Probe ◽  
High Selectivity ◽  
Fast Response ◽  
Living Cells ◽  
Fluorescence Sensor ◽  
Ratiometric Fluorescence ◽  
Ratiometric Fluorescent Probe

A ratiometric fluorescent probe CRSH based on a FRET platform for detecting HOCl. CRSH showed high selectivity, excellent sensitivity and a fast response toward HOCl.

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