Biomimetic Synthesis of Tooth-Like Hydroxyapatite under Organic Polymer Monolayer

2009 ◽  
Vol 610-613 ◽  
pp. 1054-1058
Author(s):  
Kun Tian ◽  
Hui Min Shuai ◽  
Xiao Min Yang
Keyword(s):  
Biomimetic Synthesis ◽  
Organic Polymer ◽  
Molar Ratio ◽  
Dentinal Tubule ◽  
Ftir Analysis ◽  
X Ray ◽  
Dentin Surface ◽  
Crosslinking Process ◽  
Protein Monolayer

Based on the basic theory of molecular recognition , we design a organic molecules model to induce the crystallization of hydroxyapatite to synthesized tooth-like calcium phosphate/hydroxyapatite under a controllable way in vitro. The cross-linking of collagen on the dentin surface and extraneous collagen was optimized by varying the molar ratio of N,N-(3-dimethylaminopropyl)- N'-ethyl-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) at a constant EDC concentration. CaCl2 and Na3PO4-12H2O solutions were added after the crosslinking process. X-ray photoelectron spectroscopic (XPS) and Fourier transform infrared spectroscopy (FTIR) analysis of organic protein monolayer for samples. The obtained composite were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM) as well as energy dispersive X-ray (EDX). XPS and FTIR analysis showed the surface organic compositions in experimental group is higher than that of normal dentin and decalcified dentin surface. The results showed that the dentinal tubule were blocked by neonatal hydroxyapatite layer which has a continuous structure of columns crystal with size of 10-40nm. Furthermore, there were column crystal with parallel direction inside, similar to the crystal array in the top of enamel rod. This study showed that the specific organic molecule model can be used as a potential effective crystal growth modifier.

Controlled Crystallization of Tooth-Like Hydroxyapatite under Gelatin Monolayer

2007 ◽  
Vol 330-332 ◽  
pp. 663-666
Author(s):  
Kun Tian ◽  
Dong Hua Guan ◽  
Ping Wu ◽  
Chun Peng Huang ◽  
Lin Niu ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Molar Ratio ◽  
Dentinal Tubule ◽  
X Ray Diffraction ◽  
X Ray ◽  
Whole Process ◽  
Crosslinking Process ◽  
Recognition Theory ◽  
Parallel Direction

Based on the molecular recognition theory, an organic molecules model was designed to induce the hydroxyapatite crystallization, to build a tooth-like calcium phosphate/hydroxyapatite under a controllable way in vitro. The cross-linking of collagen on the dentin surface and gelatin was optimized by varying the molar ratio of N,N-(3-dimethylaminopropyl)- N'-ethyl-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) at a constant EDC concentration. CaCl2 and Na3PO4-12H2O solutions were added after the crosslinking process. The whole process requires repeating the crosslinking and mineralization process for five times. The obtained composite were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM) as well as energy dispersive X-ray photoelectron spectroscopy (XPS). The results showed that the dentinal tubule were blocked by neonatal hydroxyapatite layer which has a continuous structure of columns crystal with size of 10-40nm. Furthermore, there was column crystal with parallel direction inside, similar to the crystal array in the top of enamel rod. This study showed that the specific organic molecule model can be used as a potential effective crystal growth modifier.

Silk Protein Assisted Ex Vivo Remineralization of Enamel-Like Microstructure

2011 ◽  
Vol 66-68 ◽  
pp. 1682-1687
Author(s):  
Kun Tian ◽  
Min Peng ◽  
Ping Wu ◽  
Chu Hang Liao ◽  
Fa Yin Huang
Keyword(s):  
Ex Vivo ◽  
Three Dimensional ◽  
Silk Protein ◽  
Molar Ratio ◽  
Dentinal Tubule ◽  
Dental Tissue ◽  
Parallel Direction ◽  
Protein Monolayer ◽  
Dental Tissue Engineering

Based on the basic theory of molecular recognition, we designed an organic molecules model that spontaneously form three-dimensional fibrillar scaffolds to induce the crystallization of hydroxyapatite to synthesized enamel-like calcium phosphate/hydroxyapatite under a controllable way in vitro. Cross-linking of collagen on the dentin surface and silk fibroin with N,N-(3-dimethylaminopropyl)-N'-ethyl-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) was optimized by varying the NHS/EDC molar ratio at constant EDC concentration. CaCl2 and Na3PO4-12H2O solution was added with Ca: P odd as 1.67:1 after conjugated. The results showed that the dentinal tubule were blocked by neonatal hydroxyapatite layer which has a continuous structure of columns crystal with size of 10-40nm. Furthermore, there were column crystal with parallel direction inside, similar to the crystal array in the top of enamel rod. The results suggest that silk protein monolayer may be useful in the modulation of mineral behavior during in situ dental tissue engineering.

scholarly journals Biomimetic Synthesis of Hydroxyapatite in Presence of Imidazole-4,5-dicarboxylic Acid Grafted Chitosan for Removing Chromium(VI)

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Zhangxu Chen ◽  
Baorong Wu ◽  
Xiaodan Huang ◽  
Xianxue Li ◽  
Yonglong Lin
Keyword(s):  
Dicarboxylic Acid ◽  
Removal Rate ◽  
Biomimetic Synthesis ◽  
Molar Ratio ◽  
Optimum Conditions ◽  
X Ray ◽  
Natural Bone ◽  
Chromium Vi ◽  
Orthogonal Experiments ◽  
Novel Method

In order to biomimetic synthesize hydroxyapatite similar to natural bone. Hydroxyapatite (HAP) is biomimetic synthesized in simulated body fluid (SBF) by addition of imidazole-4,5-dicarboxylic acid grafted chitosan (IDACS). The effect of molar ratio of chitosan (CS) to imidazole-4,5-dicarboxylic acid (IDA) on preparation of HAP was investigated. The structure, size, and crystal phase of the obtained hydroxyapatite were observed by Fourier transform infrared spectroscopy, X-ray powder diffraction, and scanning electron microscopy. The results show that the molar ratio of CS to IDA is 1 : 3, the temperature is 37.0°C, the aging time is 48 h, the synthesized nanorod-like hydroxyapatite with diameter 20–30 nm, and length ranging from 75 to 120 nm presents excellent phase, which disperses well and is similar to the natural bone of HAP. The obtained HAP can be used to remove chromium(VI) by the orthogonal experiments, and the results indicated that the removal rate can reach 95.66% under the optimum conditions. These results suggest that the morphology of the obtained HAP is more affected by the material ratio of chitosan to imidazole-4,5-dicarboxylic acid than its structure, and the obtained HAP can effectively remove Cr(VI), which provides a novel method for biomimetic synthesis of other biomaterials and application in the water purification.

scholarly journals Can Inorganic Bovine Bone Grafts Present Distinct Properties?

2014 ◽  
Vol 25 (4) ◽  
pp. 282-288 ◽  
Author(s):  
Fernanda de Paula do Desterro ◽  
Márcia Soares Sader ◽  
Glória Dulce de Almeida Soares ◽  
Guaracilei Maciel Vidigal Jr
Keyword(s):  
Surface Area ◽  
Calcium Release ◽  
Physicochemical Characteristics ◽  
Molar Ratio ◽  
Nanoporous Structure ◽  
Bovine Bone ◽  
Ftir Analysis ◽  
X Ray Diffraction ◽  
Absorption Bands ◽  
X Ray

The aim of this study was to evaluate the physicochemical characteristics of 3 mineralized bovine inorganic biomaterials and correlate them with the dissolution rate. Bio-Oss(r), GenoxInorgânico(r), and Bonefill(r) were examined using field emission gun scanning electron microscopy (FEG-SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), specific surface area (BET), calcium/phosphorous molar ratio and a dissolution assay. Bio-Oss(r) showed a micro- and nanoporous structure consisting of 15-nm hydroxyapatite (HA) crystallites; Genox(r), a microporous structure composed of 39-nm HA crystallites; and Bonefill(r), micro- and nanoporous structure of indeterminable crystallite size. FTIR analysis showed that Bio-Oss(r) and Genox(r) were composed of calcium phosphate. The absorption bands of phosphate were poorly defined in Bonefill(r). By XRD, Bio-Oss(r) was shown to contain peaks related to the carbonated HA, whereas Genox(r) only contained peaks corresponding to HA. The broad bands in Bonefill(r) indicated low crystallinity. Bio-Oss(r) showed a greater surface area and calcium release rate than that of Genox(r). Although all biomaterials were of bovine origin, the different manufacturing processes result in materials with different physicochemical properties and may influence the biological and clinical response.

scholarly journals Remineralizing Efficacy of Fluorohydroxyapatite Gel on Artificial Dentinal Caries Lesion

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Qianqian Wang ◽  
Shize Liu ◽  
Xuejun Gao ◽  
Yan Wei ◽  
Xuliang Deng ◽  
...  
Keyword(s):  
Average Diameter ◽  
Micro Computed Tomography ◽  
X Ray Diffraction ◽  
Mineral Density ◽  
Caries Lesion ◽  
X Ray ◽  
Dentin Surface ◽  
Calcium Phosphorus ◽  
Ft Ir

The aim was to evaluate the remineralizing efficacy of fluorohydroxyapatite (FHA) gel on artificial dentinal caries lesion in vitro. Artificial carious lesions were made on occlusal cavities of teeth by exposing the dentin surface to a demineralizing solution. Each cavity was capped with a 3 mm thick FHA gel for 4 weeks. After the FHA gel was removed, the surface morphology and structure of the dentin were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). The dentin mineral density (DMD) was measured by micro-computed tomography (Micro-CT). A layer of dense and orderly hexagonal crystal structure, with average diameter of 1 μm and thickness of 4~5 μm, could be observed on dentin surface. These crystals exhibited elemental peaks for calcium, phosphorus, carbon, and oxygen and characteristic peaks of hydroxyapatite (HA) and fluorapatite (FA) via XRD and FT-IR. The DMD of dentin surface layer significantly increased after it was capped with FHA gel (P<0.05). In the present study, the FHA gel could rapidly construct apatite on the artificial dentin caries surface and significantly increase the mineral density, which suggests that FHA gel might be a proper IPT material with remineralizing function.

scholarly journals Preparation and Characterization of Paclitaxel Loaded SF/PLLA-PEG-PLLA Nanoparticles via Solution-Enhanced Dispersion by Supercritical CO2

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Zheng Zhao ◽  
Yi Li ◽  
Yu Zhang
Keyword(s):  
Supercritical Co2 ◽  
Crystalline State ◽  
Tumor Therapy ◽  
Ftir Analysis ◽  
Spectroscopy Analysis ◽  
X Ray ◽  
Release Property ◽  
Ph Dependent

Paclitaxel loaded silk fibroin/PLLA-PEG-PLLA (PTX-SF/PLLA-PEG-PLLA) nanoparticles with a mean particle size of about 651 nm were fabricated successfully by the SEDS process. Fourier transform infrared (FTIR) spectroscopy analysis indicated that the PTX was encapsulated by SF/PLLA-PEG-PLLA nanoparticles. X-ray powder diffraction (XRPD) analysis supported the results of FTIR analysis and also suggested that the crystalline state of PTX was decreased obviously. Furthermore, the UV-Vis/HPLC analysis showed that drug load (DL) and encapsulation efficiency (EE) were 18.1% and 90.2%, respectively. Thein vitrodrug release experiment suggested that the PTX-SF/PLLA-PEG-PLLA nanoparticles exhibited a sustained release and only 16.1% and 24.5% of paclitaxel were released at pH 7.4 and 6.0, respectively, in one week. The PTX-SF/PLLA-PEG-PLLA nanoparticles drug delivery system with pH-dependent release property has potential application in the field of tumor therapy.

Occlusion Effect of Dentinal Tubules of the Dentifrice Containing Nano-Sized Carbonate Apatite

2007 ◽  
Vol 342-343 ◽  
pp. 821-824
Author(s):  
Su Young Lee ◽  
Sun Ok Jang ◽  
H.K. Kwon ◽  
Young Sik Cho ◽  
B.I. Kim
Keyword(s):  
Strontium Chloride ◽  
Carbonate Apatite ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Dentin Surface ◽  
Carbonated Apatite ◽  
Group 4 ◽  
Significant Difference ◽  
Group 1

Exposed dentine with patent tubules allows the movement of the tubule fluid leading to dentine sensitivity. An occlusion of patent dentinal tubules can effectively reduce the state of dentine hypersensitivity. Strontium chloride (SrCl2) has been known as a representative component of a desensitizing dentifrice. Recently, a desensitizing dentifrice containing nano-sized Carbonated Apatites (n-CAPs) was released onto the market. The aim of this in vitro study was to evaluate the occlusion effects of dentinal tubules in dentifrices containing several concentrations of the n-CAPs. One hundred human dentine specimens were embedded into a Teflon mold and ground with silicone carbide papers to expose the dentin surface. The dentin surface was etched with 37% phosphoric acid for 60 seconds and washed ultrasonically for 1 hour. The dentifrices were classified into five groups: experimental dentifrices Group 1: n-CAPs 5%, group 2: n-CAPs 15%, group 3: n- CAPs 0% and group 4: n-CAPs 30%, and the control dentifrice group 5: SrCl2 10%. The dentifrice slurries were prepared as 20 grams of dentifrice in 80 ml of distilled water. Fifty specimens were brushed with the dentifrice slurries using a V8 Cross Brushing Machine. Tooth brushing was performed 5,000 times using a back-and–forth stroke. Images showing the sizes of the dentinal tubules were taken by scanning electron microscopy (×3000) and the changes in the tubule sizes were analyzed using a digital analyzer. The n-CAPs used in this study had a high solubility and affinity to bone minerals. In addition, the n-CAPs were well deposited on patent dentinal tubules. The group 1 dentifrice (n-CAPs 5% and silica 25%) showed the highest dentinal tubule occlusion, and there was a statistically significant difference between group 4 and the control (p<0.05). Overall, the dentifrice containing 5% nano carbonated apatite and 25% silica is the most effective in occluding dentinal tubules.

scholarly journals The Amelogenin-Derived Peptide TVH-19 Promotes Dentinal Tubule Occlusion and Mineralization

Polymers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 2473
Author(s):  
Xiu Peng ◽  
Sili Han ◽  
Kun Wang ◽  
Longjiang Ding ◽  
Zhenqi Liu ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Fluorescein Isothiocyanate ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Dentinal Tubule ◽  
X Ray ◽  
Electron Microscopy Analysis ◽  
Tubule Occlusion ◽  
Demineralized Dentin

In this study, the amelogenin-derived peptide, TVH-19, which has been confirmed to promote mineralization, was evaluated to derive its potential to induce dentinal tubule occlusion. The binding capability of fluorescein isothiocyanate (FITC)-labeled TVH-19 to the demineralized dentin surface was analyzed by confocal laser scanning microscopy (CLSM). Additionally, the sealing function of the peptide was studied through the remineralization of demineralized dentin in vitro. The adsorption results showed that TVH-19 could bind to the hydroxyapatite and demineralized dentin surfaces, especially to periodontal dentin. Scanning electron microscopy analysis further revealed that TVH-19 created mineral precipitates. The plugging rate in the TVH-19 group was higher than that in the PBS group. Moreover, energy-dispersive X-ray spectroscopy (EDX) results indicated that the calcium/phosphorus (Ca/P) ratio of the new minerals induced by TVH-19 was close to that of the hydroxyapatite. Attenuated total internal reflection-Fourier transform infrared (ATR-FTIR) spectrometry and X-ray diffraction (XRD) results indicated that the hydroxyapatite crystals formed via remineralization elongated the axial growth and closely resembled the natural dentin components. These findings indicate that TVH-19 can effectively promote dentin sealing by binding to the periodontal dentin, promoting mineral deposition, and reducing the space between the dentin tubules.

scholarly journals Occluding Effect of Nd:YAG Laser and Different Dentin Desensitizing Agents on Human Dentinal Tubules In Vitro: A Scanning Electron Microscopy Investigation

2012 ◽  
Vol 37 (4) ◽  
pp. 340-355 ◽  
Author(s):  
LMS Al-Saud ◽  
HNA Al-Nahedh
Keyword(s):  
Nd:Yag Laser ◽  
Ethylenediaminetetraacetic Acid ◽  
In Vitro Study ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Dentin Surface ◽  
Desensitizing Agents ◽  
The One ◽  
Scanning Electron

SUMMARY Objectives This in vitro study aimed to microscopically evaluate and compare the occluding effect of the Nd:YAG laser and different dentin desensitizing agents on human dentinal tubules. Materials and Methods The Nd:YAG laser (SunLase™ 800) and four commercially available and professionally applied dentin desensitizers (Gluma® desensitizer, Tenure Quick®, Quell™ desensitizer, and VivaSens®) were investigated in this study. Sixty-four extracted intact human molars were used. Each dentin surface was divided by shallow indentation into two halves, one of which was used for treatment and the other of which served as a control. The dentin surfaces were etched to remove any smear plugs and to mimic the open dentinal tubules of sensitive dentin using 0.5 M ethylenediaminetetraacetic acid (pH 7.4) for two minutes (applied with a microbrush) and then rinsed with an air-water syringe for 30 seconds. The laser samples (n=16) were randomly divided into four groups of four samples each. These groups were the step-up technique group, the 14-day group, the one-minute group, and the two-minute group. Forty-eight samples were treated with the four tested desensitizing agents and were randomly divided into four groups (n=12/group). Each group was further subdivided into three subgroups (n=4). Samples of the first subgroup were treated for 14 days, while those of the second subgroup were treated once. Samples of the last subgroup were fractured longitudinally after a single treatment. All of the samples were then examined under a scanning electron microscope. Results The Nd:YAG laser–irradiated dentin showed reduction or complete obliteration of the dentinal tubule lumen; thus, the treatment modified the original dentinal structure. The lased dentin surface in the two-minute group showed bubble-like changes in the area of the dentinal tubules' orifices. Statistically, the two-minute group was found to have a significantly higher percentage of partially or fully occluded tubules than did the one-minute group. All of the studied desensitizing agents produced occlusion of the dentinal tubules; however, the appearance of the precipitates, the level of coverage, and the degree of dentinal occlusion varied among the tested products. Conclusion Throughout the specified period of this study, occlusion and/or narrowing of the open dentinal tubules have been successfully achieved with both treatment approaches.

Induced Synthesis of Hydroxyapatite by Chitosan for Enamel Remineralization

2012 ◽  
Vol 530 ◽  
pp. 40-45 ◽  
Author(s):  
Kun Tian ◽  
Min Peng ◽  
Wei Fei ◽  
Chu Hang Liao ◽  
Xiao Hua Ren
Keyword(s):  
Calcium Fluoride ◽  
Dentinal Tubule ◽  
Dental Tissue ◽  
Crosslinking Process ◽  
Parallel Direction ◽  
Biomimetic Method ◽  
Dental Tissue Engineering ◽  
In Situ Crosslinking

The formation of organized nanocrystals that resemble tooth-like hydroxyapatite is crucial for successful enamel remineralization. Based on the principles of biomineralization , spindle - shaped hydroxyapatites (HA) were synthesized through biomimetic method with chitosan as template under a controllable way in vitro. We observed that hydroxyapatite nanorods can be controlled followed by in situ crosslinking process and triggered by conditions of pH and ionic strength. The dentinal tubule were blocked by neonatal hydroxyapatite layer and this composite a continuous structure of columns crystal with size of 10-40nm. At the same time, XRD showed that the precipitation was calcium fluoride phosphate and Ca:P was 1.6. Furthermore, there were column crystal with parallel direction inside, as same as the crystal array in the top of enamel rod. The results suggest that chitosan monolayer may be useful in the modulation of mineral behavior during in situ dental tissue engineering.

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