Effect of Nano Silver on Inducement of Differentiation of Neural Stem Cells of SD Rats

2016 ◽  
Vol 852 ◽  
pp. 1243-1249
Author(s):  
Xiao Qian Yu ◽  
Li Xin Xu
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Intervention Group ◽  
Culture Method ◽  
Control Group ◽  
Sd Rat ◽  
Sd Rats ◽  
The Status ◽  
Astrocyte Differentiation ◽  
Normal Differentiation

Objective: To explore the effect of nanosilver on inducement of differentiation of neural stem cells of SD rats. Method: nanosilver film and newborn SD rat neural stem cell co-culture method is adopted to perform primary and subculture on SD rat neural stem cells and to induce differentiation. Nestin staining is adopted to identify the characteristics of neural stem cells. Immunohistochemistry method (β-III-tubulin, GFAP staining) is adopted to detect the status of differentiation from neural stem cells into neurons and neurogliocytes. Result: The neural stem cells of newborn SD rats can form Nestin positive cell balls in case of no serum culture. After induction of differentiation, NF-positive cells and GFAP-positive cells can be seen through immunofluorescence staining, wherein for nanosilver intervention group, the proportion of hippocampal NSCs differentiating to group neurons is smaller than that of the control group, with significantly reduced impact on astrocyte differentiation. Conclusion: nanosilver has adverse effect on normal differentiation of hippocampal NSCs to neurons and astrocytes.

scholarly journals Neuroprotective effects of dexmedetomidine on the ketamine-induced disruption of the proliferation and differentiation of developing neural stem cells in the subventricular zone

2020 ◽  
Author(s):  
Huanhuan Sha ◽  
Peipei Peng ◽  
Bing Li ◽  
Guohua Wei ◽  
Juan Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Subventricular Zone ◽  
Pediatric Anesthesia ◽  
Brdu Incorporation ◽  
Control Group ◽  
Neuroprotective Effects ◽  
Proliferation And Differentiation ◽  
Ketamine Anesthesia ◽  
Β Tubulin

Abstract Background: Recently, the number of neonatal patients receiving surgery under general anesthesia has increased. Ketamine disrupts the proliferation and differentiation of developing neural stem cells (NSCs). Therefore, the safe use of ketamine in pediatric anesthesia has been an issue of increasing concern among anesthesiologists and the children’s parents. Dexmedetomidine (DEX) is widely used in sedation, as an antianxiety agent and for analgesia. DEX has recently been shown to provide neuroprotection against anesthetic-induced neurotoxicity in the developing brain. The aim of this in vivo study was to investigate whether DEX exerted neuroprotective effects on the proliferation and differentiation of NSCs in the subventricular zone (SVZ) following neonatal ketamine exposure. Methods: Postnatal day 7 (PND-7) male Sprague-Dawley rats were equally divided into the following 5 groups: Control group (n=8), Ketamine group (n=8), 1 μg/kg DEX+Ketamine group (n=8), 5 μg/kg DEX+Ketamine group (n=8) and 10 μg/kg DEX+Ketamine group (n=8). The proliferation and differentiation of NSCs in the SVZ were assessed using immunostaining with BrdU incorporation. The levels of Nestin and β-tubulin III in the SVZ were measured using Western blot analyses. Apoptosis was assessed by detecting the levels of the cleaved caspase-3 protein using Western blotting. Results: Neonatal ketamine exposure significantly inhibited NSC proliferation and astrocytic differentiation in the SVZ, and neuronal differentiation was markedly increased. Furthermore, pretreatment with moderate (5 μg/kg) or high doses (10 μg/kg) of DEX reversed the ketamine-induced disturbances in the proliferation and differentiation of NSCs. Meanwhile, neonatal ketamine exposure significantly decreased the expression of Nestin and increased the expression of β-tubulin III in the SVZ compared with the Control group. Treatment with 10 μg/kg DEX notably reversed the ketamine-induced changes in the levels of Nestin and β-tubulin III. In addition, a pretreatment with 10 μg/kg DEX before ketamine anesthesia prevented apoptosis in the SVZ induced by neonatal ketamine exposure. Conclusions: Based on our findings, DEX may exert neuroprotective effects on the proliferation and differentiation of NSCs in the SVZ of neonatal rats in a repeated ketamine anesthesia model.

EGF-responsive rat neural stem cells: Molecular follow-up of neuron and astrocyte differentiation in vitro

2003 ◽  
Vol 195 (2) ◽  
pp. 220-233 ◽  
Author(s):  
F.P. Jori ◽  
U. Galderisi ◽  
E. Piegari ◽  
M. Cipollaro ◽  
A. Cascino ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Astrocyte Differentiation

scholarly journals Chaihu-Shugan-San Decoction Modulates Intestinal Microbe Dysbiosis and Alleviates Chronic Metabolic Inflammation in NAFLD Rats via the NLRP3 Inflammasome Pathway

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Yinji Liang ◽  
Yupei Zhang ◽  
Yuanjun Deng ◽  
Shu Liang ◽  
Yifang He ◽  
...  
Keyword(s):  
Nlrp3 Inflammasome ◽  
High Fat Diet ◽  
Intervention Group ◽  
Control Group ◽  
Inflammatory Factor ◽  
High Fat ◽  
Metabolic Inflammation ◽  
Reduced Body ◽  
Sd Rats ◽  
Total Fat

We evaluate the effects of the Chaihu-Shugan-San decoction on intestinal microbe dysbiosis and chronic metabolic inflammation via the NLRP3 pathway in NAFLD rats that were fed a high-fat diet. Twenty-four SD rats (male, six weeks old, 200 ± 20 g) were randomly divided into three groups: normal control group (NC group), high-fat diet-fed group (HFD group), and Chaihu-Shugan-San decoction intervention group (CH group). The NC group rats were given standard feed, the HFD group rats were all fed a high-fat diet (83% standard feed + 10% lard oil + 5% sucrose + 1.5% cholesterol + 0.5% cholate), and the CH group rats were given a HFD plus Chaihu-Shugan-San at 9.6 g•kg−1•d−1. Body composition, serum and liver lipids, inflammatory markers, intestinal microbial population, and the NLRP3 pathway-associated protein were assessed. The results showed that Chaihu-Shugan-San decoction significantly reduced body weight and total fat mass and the levels of serum LPS, TG, TNF-α, IL-1β, and IL-18, as well as liver TC, TG, TNF-α, IL-1β, and IL-18 (P <0.05). The abundance of Enterobacteriaceae (0.375% versus 0.064%,P< 0.05), Staphylococcaceae families (0.049% versus 0.016%,P< 0.05) andVeillonellagenus (0.096% versus 0.009%,P <0.01) significantly decreased, whereas the abundance ofAnaeroplasmagenus (0.0005% versus 0.0178%,P <0.01) significantly increased. The expression levels of NLRP3, ASC, and Caspase-1 were changed significantly (P< 0.05). In summary, the Chaihu-Shugan-San decoction modulated intestinal microbe dysbiosis, reduced fat accumulation, and alleviated inflammatory factor expression, which are all processes related to the NLRP3 inflammasome pathway in NAFLD rats.

scholarly journals Gliotypic Neural Stem Cells Transiently Adopt Tumorigenic Properties During Normal Differentiation

Stem Cells ◽  
2009 ◽  
Vol 27 (2) ◽  
pp. 280-289 ◽  
Author(s):  
Noah M. Walton ◽  
Gregory E. Snyder ◽  
Donghyun Park ◽  
Firas Kobeissy ◽  
Bjorn Scheffler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Normal Differentiation

scholarly journals The intervention of maternal nutrition literacy has the potential to prevent childhood stunting: Randomized control trials

2021 ◽  
Vol 10 (2) ◽  
Author(s):  
Sirajuddin Sirajuddin ◽  
Saifuddin Sirajuddin ◽  
Amran Razak ◽  
Ansariadi Ansariadi ◽  
Ridwan M Thaha ◽  
...  
Keyword(s):  
Maternal Nutrition ◽  
Stress Test ◽  
Intervention Group ◽  
Control Group ◽  
Normal Children ◽  
Before And After ◽  
The Status ◽  
Randomized Control ◽  
Families With Children ◽  
The Impact

Background: Stunting is the impaired growth and development of children due to poor nutrition, repeated infection, and inadequate psychological stimulation. This research aims to examine the impact of maternal nutrition literacy (MNL) in increasing the height or score of a stunted child.Design and Methods: This study is a randomized control trial, which uses a sample size of 85 participants, 43 interventions and 42 controls, an 80% stress test and a 95% confidence level. The intervention group of the MNL consists of families with children under the age of five, focused on the mother's ability to perform breastfeeding, hygiene activities, care, and intervention for 3 months.Result: The status of stunting was determined by the different distribution of stunting before and after the intervention in both the intervention and control groups. There was a decrease of about 9.3% of MNL in the intervention group, while in the control group it decreased by just 2.4% (p<0.05).Conclusions: It can be concluded that MNL has an effect in preventing stunting, and it is recommended that preventive measures should focus more on normal children, while stunted children should be provided with breastfeeding as the core of MNL.

scholarly journals Potential role of heat shock protein 90 in regulation of hyperthermia-driven differentiation of neural stem cells

2019 ◽  
Author(s):  
Lei Wang ◽  
Yi Zhuo ◽  
Zhengwen He ◽  
Ying Xia ◽  
Ming Lu
Keyword(s):  
Stem Cells ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Neural Stem Cells ◽  
Time Window ◽  
Heat Shock Protein 90 ◽  
Hsp90 Inhibitor ◽  
Control Group ◽  
Transcriptional Level ◽  
Hyperthermic Treatment

AbstractObjectiveOur previous studies indicated that hyperthermia may play a role in differentiation of neural stem cells and that hypoxia inducible factor-1(HIF-1) was critical in this process. Heat shock protein 90 (Hsp90) is one of the most common heat-related proteins and involved in HIF-1 expression by regulating its activity and stabilization. Here, we hypothesized that HSP90 may be involved in regulation of hyperthermia-driven differentiation of neural stem cells(NSCs). We also investigated whether the HSP90 activity exert its regulatory action via HIF-1 pathway and the transcriptional level of the target genes of HIF-1.MethodThe cultured NSCs were divided into three groups: an hyperthermic treatment group(40NSC) which NSCs was induced under 40°C temperature; a control group(37NSC) which NSCs was induced under 37°C temperature; an hyperthermic treatment and HSP90-inhibited group(40NSC+GA) which NSCs was induced with 0.5μM HSP90 inhibitor Geldanamycin(GA) under 40°C temperature. We examined cells HSPa and HIF-1a expression during a time window of 5 days(12h, 1d, 3d, 5d) post-differentiation. The expression HSPα, HIF-1α, VEGF (vascular endothelial growth factor) and erythmpoietin(EPO) of during a time window was evaluated by RT-qPCR. The proportion of Tuj-1 positive differentiated cells were observed by flow cytometry.ResultHyperthermia promoted neuronal differentiation of NSC, and this effect could be blocked by HSP90 inhibitor GA. We observed the up-regulation of HSP90 during hyperthermia treatment, and that the protein levels of HIF-1α changed depending of the GA treatment. GA could not inhibited HSP90α expression but suppressed HSP activity and decreased the expression HIF-1α protein. Inhibition of HIF-1α expression by GA could consequently affect expression of its targeted genes such as VEGF and EPO.ConclusionHyperthermia promote differentiation of NSCs into neurons. HSP90 involved in the regulation of hyperthermia-driven differentiation of NSC, and the mechanism is related to HIF-1α and its downstream gene activation.

scholarly journals Allogeneic diabetic mesenchymal stem cells transplantation in streptozotocin-induced diabetic rat

2008 ◽  
Vol 31 (6) ◽  
pp. 328 ◽  
Author(s):  
Qing-Yu Dong ◽  
Li Chen ◽  
Guan-Qi Gao ◽  
Lei Wang ◽  
Jun Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Body Weight ◽  
Blood Glucose ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Control Group ◽  
Pancreatic Regeneration ◽  
Insulin Producing Cells ◽  
The Status

Background: Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stroma cells which can provide a potential therapy for diabetes mellitus. But the mechanism is still controversial. Also, the status of BM-MSCs under hyperglycemia is not known. In the present study, we investigated the status of BM-MSCs in experimental-diabetic rat and demonstrated the rescue of experimental diabetes by diabetic MSCs transplantation. Methods: BM-MSCs were cultured and the potential of multiple-differentiation was identified through induction into osteoblasts. MSCs of passage 3 were used for the following experiment. The MSCs were labeled with 5-bromo-2?-deoxyuridine (BrdU). Diabetes in rats was induced by STZ injection. The rats were divided into three groups: normal control group (no DM, rats treated with saline through tail vein, n=10); DM control group (DM, no transplantation of MSCs, n=20); experimental group (DM and transplantation of MSCs, n=20). Body weight and blood glucose of the rats were monitored during the experiment after transplantation of MSCs. Paraffin sections of pancreas were obtained from rats of each group. Immuno-histochemistry analysis and double immunofluorescence were used to detect the BM-MSCs in the pancreatic tissue and their differentiating state. Results: MSCs were 89.5% labeled by BrdU and DAPI, which was green/blue double stained under fluorescent microscopy. Transplantation of diabetic MSCs resulted in a reduction of hyperglycemia on day 45 in experimental diabetic rats compared with control rats (17.7 mM ±3.9 vs 27.8 mM ± 2.1, P < 0.05), There was also a difference between MSC-treated experimental diabetic rats and control rats in body weight (232.7 g ±19.7 vs 133.3g ±13.1, P < 0.05). Histological and morphometric analysis of the pancreas of experimental diabetic rats showed the presence and differentiation of transplanted MSCs into insulin-producing cells which evidenced by double-staining of anti-BrdU and insulin. Also, there were many small islets throughout the sections. Their mean area and diameter analysis revealed that they were smaller thancontrol islets (1835.7 ± 175.8 µm2 vs 13257.2 ± 1457.6 µm2; 43.5 ± 3.7 µm vs 119.9 ± 5.8 µm, respectively, P < 0.05). Conclusion: Allogeneic MSCs transplantation can reduce blood glucose level in recipient rats. A relatively small quantity of transplanted diabetic MSCs survive and transdifferentiate into insulin-producing cells in the pancreas of recipient rats. Upon transplantation these cells initiate endogenous pancreatic regeneration by neogenesis of islet of recipient origin. The present study demonstrates that diabetic MSCs retains its stemness and potential to induce pancreatic regeneration on transplantation.

Identification of the gliogenic state of human neural stem cells to optimize in vitro astrocyte differentiation

2021 ◽  
pp. 109284
Author(s):  
Marlen Alisch ◽  
Janis Kerkering ◽  
Tadhg Crowley ◽  
Kamil Rosiewicz ◽  
Friedemann Paul ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Human Neural Stem Cells ◽  
Astrocyte Differentiation
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