scholarly journals In-Situ Shadowgraph Imaging

2018 ◽  
Vol 52 (6) ◽  
pp. 62-65
Author(s):  
Adam T. Greer

AbstractVarious imaging systems are producing complex data that improve our understanding of physical and biological coupling in the ocean. In-situ shadowgraph imaging is one sampling technique that captures detailed biological information for a wide range of plankton with differing body compositions (e.g., gelatinous to crustacean) in their natural orientations, as well as small-scale physical changes. When combined with rapidly advancing computer vision methods and high-resolution oceanographic observations, shadowgraph imagery holds promise for generating new discoveries in ocean science that are only limited by our creativity in asking new questions.

2000 ◽  
Vol 663 ◽  
Author(s):  
J. Samper ◽  
R. Juncosa ◽  
V. Navarro ◽  
J. Delgado ◽  
L. Montenegro ◽  
...  

ABSTRACTFEBEX (Full-scale Engineered Barrier EXperiment) is a demonstration and research project dealing with the bentonite engineered barrier designed for sealing and containment of waste in a high level radioactive waste repository (HLWR). It includes two main experiments: an situ full-scale test performed at Grimsel (GTS) and a mock-up test operating since February 1997 at CIEMAT facilities in Madrid (Spain) [1,2,3]. One of the objectives of FEBEX is the development and testing of conceptual and numerical models for the thermal, hydrodynamic, and geochemical (THG) processes expected to take place in engineered clay barriers. A significant improvement in coupled THG modeling of the clay barrier has been achieved both in terms of a better understanding of THG processes and more sophisticated THG computer codes. The ability of these models to reproduce the observed THG patterns in a wide range of THG conditions enhances the confidence in their prediction capabilities. Numerical THG models of heating and hydration experiments performed on small-scale lab cells provide excellent results for temperatures, water inflow and final water content in the cells [3]. Calculated concentrations at the end of the experiments reproduce most of the patterns of measured data. In general, the fit of concentrations of dissolved species is better than that of exchanged cations. These models were later used to simulate the evolution of the large-scale experiments (in situ and mock-up). Some thermo-hydrodynamic hypotheses and bentonite parameters were slightly revised during TH calibration of the mock-up test. The results of the reference model reproduce simultaneously the observed water inflows and bentonite temperatures and relative humidities. Although the model is highly sensitive to one-at-a-time variations in model parameters, the possibility of parameter combinations leading to similar fits cannot be precluded. The TH model of the “in situ” test is based on the same bentonite TH parameters and assumptions as for the “mock-up” test. Granite parameters were slightly modified during the calibration process in order to reproduce the observed thermal and hydrodynamic evolution. The reference model captures properly relative humidities and temperatures in the bentonite [3]. It also reproduces the observed spatial distribution of water pressures and temperatures in the granite. Once calibrated the TH aspects of the model, predictions of the THG evolution of both tests were performed. Data from the dismantling of the in situ test, which is planned for the summer of 2001, will provide a unique opportunity to test and validate current THG models of the EBS.


Author(s):  
Klas Ove MÖller ◽  
Michael St. John ◽  
Axel Temming ◽  
Rabea Diekmann ◽  
Janna Peters ◽  
...  

Abstract Predators not only have direct impact on biomass but also indirect, non-consumptive effects on the behavior their prey organisms. A characteristic response of zooplankton in aquatic ecosystems is predator avoidance by diel vertical migration (DVM), a behavior which is well studied on the population level. A wide range of behavioral diversity and plasticity has been observed both between- as well as within-species and, hence, investigating predator–prey interactions at the individual level seems therefore essential for a better understanding of zooplankton dynamics. Here we applied an underwater imaging instrument, the video plankton recorder (VPR), which allows the non-invasive investigation of individual, diel adaptive behavior of zooplankton in response to predators in the natural oceanic environment, providing a finely resolved and continuous documentation of the organisms’ vertical distribution. Combing observations of copepod individuals observed with the VPR and hydroacoustic estimates of predatory fish biomass, we here show (i) a small-scale DVM of ovigerous Pseudocalanus acuspes females in response to its main predators, (ii) in-situ observations of a direct short-term reaction of the prey to the arrival of the predator and (iii) in-situ evidence of pronounced individual variation in this adaptive behavior with potentially strong effects on individual performance and ecosystem functioning.


2015 ◽  
Vol 15 (4) ◽  
pp. 4725-4766 ◽  
Author(s):  
Fuqing Zhang ◽  
Junhong Wei ◽  
Meng Zhang ◽  
K. P. Bowman ◽  
L. L. Pan ◽  
...  

Abstract. This study analyzes in situ airborne measurements from the 2008 Stratosphere–Troposphere Analyses of Regional Transport (START08) experiment to characterize gravity waves in the extratropical upper troposphere and lower stratosphere (ExUTLS) region. The focus is on the second research flight (RF02), which took place on 21–22 April 2008. This was the first airborne mission dedicated to probing gravity waves associated with strong upper-tropospheric jet-front systems. Based on spectral and wavelet analyses of the in situ observations, along with a diagnosis of the polarization relationships, clear signals of mesoscale variations with wavelengths ~50–500 km are found in almost every segment of the 8 h flight, which took place mostly in the lower stratosphere. The aircraft sampled a wide range of background conditions including the region near the jet core, the jet exit and over the Rocky Mountains. In contrast to the long wavelength mesoscale variations, smaller-scale wavelike oscillations below 50 km are found to be quite transient. In particular, aircraft measurements of several flight segments are dominated by signals with periods of ~20– ~60 s and wavelengths of ~5– ~15 km. We speculate that at least part of these nearly-periodic high-frequency signals are a result of intrinsic observational errors in the aircraft measurements or small-scale flight-altitude fluctuations that are difficult to fully characterize. Despite the presence of possibly spurious wave oscillations in several flight segments, the power spectra of horizontal winds and temperature averaged over the analyzed START08 flight segments follow closely the -5/3 power law.


2019 ◽  
Vol 49 (9) ◽  
pp. 2447-2468
Author(s):  
Leonel Romero ◽  
J. Carter Ohlmann ◽  
Enric Pallàs-Sanz ◽  
Nicholas M. Statom ◽  
Paula Pérez-Brunius ◽  
...  

AbstractCoincident Lagrangian observations of coastal circulation with surface drifters and dye tracer were collected to better understand small-scale physical processes controlling transport and dispersion over the inner shelf in the Gulf of Mexico. Patches of rhodamine dye and clusters of surface drifters at scales of O(100) m were deployed in a cross-shelf array within 12 km from the coast and tracked for up to 5 h with airborne and in situ observations. The airborne remote sensing system includes a hyperspectral sensor to track the evolution of dye patches and a lidar to measure directional wavenumber spectra of surface waves. Supporting in situ measurements include a CTD with a fluorometer to inform on the stratification and vertical extent of the dye and a real-time towed fluorometer for calibration of the dye concentration from hyperspectral imagery. Experiments were conducted over a wide range of conditions with surface wind speed between 3 and 10 m s−1 and varying sea states. Cross-shelf density gradients due to freshwater runoff resulted in active submesoscale flows. The airborne data allow characterization of the dominant physical processes controlling the dispersion of passive tracers such as freshwater fronts and Langmuir circulation. Langmuir circulation was identified in dye concentration maps on most sampling days except when the near surface stratification was strong. The observed relative dispersion is anisotropic with eddy diffusivities O(1) m2 s−1. Near-surface horizontal dispersion is largest along fronts and in conditions dominated by Langmuir circulation is larger in the crosswind direction. Surface convergence at fronts resulted in strong vertical velocities of up to −66 m day−1.


Author(s):  
Charlie Jarvis ◽  
Emily Webster ◽  
Stuart Davidson ◽  
Ian Robinson

Kibble balance experiments have allowed the kilogram to be redefined in terms of the Planck constant. Now that the redefinition is in place, the Kibble balance will allow SI traceable mass (or force) to be realised at any value and at any location. A feasibility study for a novel, scalable electrostatic ‘µKibble balance’, based on the National Physical Laboratory (NPL) next-generation Kibble balance system is presented and its expected performance calculated. A µKibble will allow in-situ, dynamic, small-scale (< g) mass measurements without the current precision limitation caused by subdivision of the kilogram. The measurements will be traceable through electrical calibration rather than transferable mass standards. The instrument will have a wide range of applications in both industry and research.


Author(s):  
W. E. King

A side-entry type, helium-temperature specimen stage that has the capability of in-situ electrical-resistivity measurements has been designed and developed for use in the AEI-EM7 1200-kV electron microscope at Argonne National Laboratory. The electrical-resistivity measurements complement the high-voltage electron microscope (HVEM) to yield a unique opportunity to investigate defect production in metals by electron irradiation over a wide range of defect concentrations.A flow cryostat that uses helium gas as a coolant is employed to attain and maintain any specified temperature between 10 and 300 K. The helium gas coolant eliminates the vibrations that arise from boiling liquid helium and the temperature instabilities due to alternating heat-transfer mechanisms in the two-phase temperature regime (4.215 K). Figure 1 shows a schematic view of the liquid/gaseous helium transfer system. A liquid-gas mixture can be used for fast cooldown. The cold tip of the transfer tube is inserted coincident with the tilt axis of the specimen stage, and the end of the coolant flow tube is positioned without contact within the heat exchanger of the copper specimen block (Fig. 2).


2013 ◽  
Vol 16 (1) ◽  
pp. 59-67

<p>The Soil Science Institute of Thessaloniki produces new digitized Soil Maps that provide a useful electronic database for the spatial representation of the soil variation within a region, based on in situ soil sampling, laboratory analyses, GIS techniques and plant nutrition mathematical models, coupled with the local land cadastre. The novelty of these studies is that local agronomists have immediate access to a wide range of soil information by clicking on a field parcel shown in this digital interface and, therefore, can suggest an appropriate treatment (e.g. liming, manure incorporation, desalination, application of proper type and quantity of fertilizer) depending on the field conditions and cultivated crops. A specific case study is presented in the current work with regards to the construction of the digitized Soil Map of the regional unit of Kastoria. The potential of this map can easily be realized by the fact that the mapping of the physicochemical properties of the soils in this region provided delineation zones for differential fertilization management. An experiment was also conducted using remote sensing techniques for the enhancement of the fertilization advisory software database, which is a component of the digitized map, and the optimization of nitrogen management in agricultural areas.</p>


2020 ◽  
Vol 24 (8) ◽  
pp. 900-908
Author(s):  
Ram Naresh Yadav ◽  
Amrendra K Singh ◽  
Bimal Banik

Numerous O (oxa)- and S (thia)-glycosyl esters and their analogous glycosyl acids have been accomplished through stereoselective glycosylation of various peracetylated bromo sugar with benzyl glycolate using InBr3 as a glycosyl promotor followed by in situ hydrogenolysis of resulting glycosyl ester. A tandem glycosylating and hydrogenolytic activity of InBr3 has been successfully investigated in a one-pot procedure. The resulting synthetically valuable and virtually unexplored class of β-CMGL (glycosyl acids) could serve as an excellent potential chiral auxiliary in the asymmetric synthesis of a wide range of enantiomerically pure medicinally prevalent β-lactams and other bioactive molecules of diverse medicinal interest.


2020 ◽  
Vol 7 (1) ◽  
pp. 9 ◽  
Author(s):  
Shelina Bhamani ◽  
Areeba Zainab Makhdoom ◽  
Vardah Bharuchi ◽  
Nasreen Ali ◽  
Sidra Kaleem ◽  
...  

<p align="center"><em>The widespread prevalence of COVID-19 pandemic has affected academia and parents alike. Due to the sudden closure of schools, students are missing social interaction which is vital for better learning and grooming while most schools have started online classes. This has become a tough routine for the parents working online at home since they have to ensure their children’s education. The study presented was designed to explore the experiences of home learning in times of COVID-19. A descriptive qualitative study was planned to explore the experiences of parents about home learning and management during COVID-19 to get an insight into real-life experiences.  Purposive sampling technique was used for data collection.  Data were collected from 19 parents falling in the inclusion criteria. Considering the lockdown problem, the data were collected via Google docs form with open-ended questions related to COVID-19 and home learning. Three major themes emerged after the data analysis: impact of COVID on children learning; support given by schools; and strategies used by caregivers at home to support learning. It was analyzed that the entire nation and academicians around the world have come forward to support learning at home offering a wide range of free online avenues to support parents to facilitate home-learning. Furthermore, parents too have adapted quickly to address the learning gap that have emerged in their children’s learning in these challenging times. Measures should be adopted to provide essential learning skills to children at home. Centralized data dashboards and educational technology may be used to keep the students, parents and schools updated.</em></p>


2020 ◽  
Vol 154 (2) ◽  
pp. 135-153 ◽  
Author(s):  
Gabriel García Caballero ◽  
Donella Beckwith ◽  
Nadezhda V. Shilova ◽  
Adele Gabba ◽  
Tanja J. Kutzner ◽  
...  

Abstract The concept of biomedical significance of the functional pairing between tissue lectins and their glycoconjugate counterreceptors has reached the mainstream of research on the flow of biological information. A major challenge now is to identify the principles of structure–activity relationships that underlie specificity of recognition and the ensuing post-binding processes. Toward this end, we focus on a distinct feature on the side of the lectin, i.e. its architecture to present the carbohydrate recognition domain (CRD). Working with a multifunctional human lectin, i.e. galectin-3, as model, its CRD is used in protein engineering to build variants with different modular assembly. Hereby, it becomes possible to compare activity features of the natural design, i.e. CRD attached to an N-terminal tail, with those of homo- and heterodimers and the tail-free protein. Thermodynamics of binding disaccharides proved full activity of all proteins at very similar affinity. The following glycan array testing revealed maintained preferential contact formation with N-acetyllactosamine oligomers and histo-blood group ABH epitopes irrespective of variant design. The study of carbohydrate-inhibitable binding of the test panel disclosed up to qualitative cell-type-dependent differences in sections of fixed murine epididymis and especially jejunum. By probing topological aspects of binding, the susceptibility to inhibition by a tetravalent glycocluster was markedly different for the wild-type vs the homodimeric variant proteins. The results teach the salient lesson that protein design matters: the type of CRD presentation can have a profound bearing on whether basically suited oligosaccharides, which for example tested positively in an array, will become binding partners in situ. When lectin-glycoconjugate aggregates (lattices) are formed, their structural organization will depend on this parameter. Further testing (ga)lectin variants will thus be instrumental (i) to define the full range of impact of altering protein assembly and (ii) to explain why certain types of design have been favored during the course of evolution, besides opening biomedical perspectives for potential applications of the novel galectin forms.


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