Influence of Acrylic Resin Denture Base Soaking Length in Siwak Extract Solution (Salvadora persica) on the Growth of Candida albicans

Maintaining acrylic denture hygiene is a necessary to prohibit the growth of several oral fungi such as Candida albicans. Candida albicans are the main etiological microorganism of denture stomatitis. One of the ways to clean denture is by immersion on synthetic denture cleaner liquid such as oxygenizing denture cleaners. However, synthetic denture cleaner have several limitation due to its biocompatibility on human body especially oral tissue. Chitosan is a derivate of chitin, a natural compound of sea creature exosceleton. Chitosan have antifungi and antibacterial nature. The objective of this study is to evaluate the antifungi efectifity of chitosan 2% on acrylic heatcured and compare it with oxygenizing denture cleaner. Aquades is used as a control. The sample consists of 15 acrylic heatcured bars (10x10x2 mm) prepared by contamination on Candida albicans suspensions at temperature 37°C for 24 hours and immersed on 3 different treatments wich are chitosan 2%, oxygenizing denture cleanser, and aquades as control and then, the solutions are being vibrate and put into sabouraud dextrose agar medium. The colony of Candida albicans counted manually. Result shows, highest mean of Candida albicans colony on aquades group (365,66 CFU/plate), followed by oxygenizing denture cleanser treatment (178,33 CFU/plate). The smallest number colony of Candida albicans are found at chitosan 2% treatment (45,33 CFU/plate). Statistical analysis using ANOVA one way shows F=9.826 and p=0, this means, there is minimal one pair of groups with very significant differential (p<0,01) which are chitosan 2% and oxygenizing denture cleanser compared to aquades as control. The difference between those two treatments also significant. Conclusions of this study is that chitosan 2% solution have a sifnificant effect on prohibiting the growth of Candida albicans colony on acrylic heatcured denture base materials. The effectivity of this solution is significantly higher than oxygenizing denture cleanser treatment.

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Evaluation of Candida albicans adhesion and biofilm formation on a denture base acrylic resin containing silver nanoparticles

Journal of Applied Microbiology ◽

10.1111/j.1365-2672.2012.05293.x ◽

2012 ◽

Vol 112 (6) ◽

pp. 1163-1172 ◽

Cited By ~ 56

Author(s):

A.F. Wady ◽

A.L. Machado ◽

V. Zucolotto ◽

C.A. Zamperini ◽

E. Berni ◽

...

Keyword(s):

Silver Nanoparticles ◽

Candida Albicans ◽

Biofilm Formation ◽

Acrylic Resin ◽

Denture Base

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Effect of Denture Base Fabrication Technique on Candida albicans Adhesion In Vitro

Materials ◽

10.3390/ma14010221 ◽

2021 ◽

Vol 14 (1) ◽

pp. 221

Author(s):

Avi Meirowitz ◽

Arkadi Rahmanov ◽

Eti Shlomo ◽

Helena Zelikman ◽

Eran Dolev ◽

...

Keyword(s):

Surface Roughness ◽

Candida Albicans ◽

3D Printing ◽

Oral Candidiasis ◽

Acrylic Resin ◽

Denture Base ◽

Denture Stomatitis ◽

Heat Curing ◽

Vitro Effect

Denture stomatitis is a common manifestation of oral candidiasis affecting some 65% of denture wearers. This condition is initiated by the adherence of Candida albicans to denture base acrylic resin. The present study aimed to test the in vitro effect of traditional and novel fabrication methods on Candida albicans adhesion to denture base samples. Denture based acrylic discs were fabricated using: (i) computerized milling, (ii) 3D printing, (iii) heat curing, and (iv) cold curing. Discs were tested for surface roughness (Ra), hydrophobicity (contact angle), mucin adsorption (Bradford assay), and Candida albicans adhesion. 3D printing significantly increased microbial cell adhesion as compared with heat curing, and computerized milling significantly decreased it. These results were associated with mucin adsorption levels rather than surface roughness. Results suggest that 3D printing may increase the risk for developing denture stomatitis, whereas computerized milling may decrease it as compared with traditional heat curing denture base fabrication.

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An in vitro study on effect of Delmopinol application on Candida albicans adherence on heat cured denture base acrylic resin: A thorough study

Indian Journal of Dental Research ◽

10.4103/0970-9290.123423 ◽

2013 ◽

Vol 24 (5) ◽

pp. 645 ◽

Cited By ~ 4

Author(s):

Deshraj Jain ◽

Prabha Shakya

Keyword(s):

Candida Albicans ◽

In Vitro Study ◽

Acrylic Resin ◽

Vitro Study ◽

Denture Base

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The effects of silane-SiO2 nanocomposite films on Candida albicans adhesion and the surface and physical properties of acrylic resin denture base material

Journal of Prosthetic Dentistry ◽

10.1016/j.prosdent.2014.06.019 ◽

2014 ◽

Vol 112 (6) ◽

pp. 1530-1538 ◽

Cited By ~ 13

Author(s):

Sirasa Yodmongkol ◽

Rojcharin Chantarachindawong ◽

Sroisiri Thaweboon ◽

Boonyanit Thaweboon ◽

Taweechai Amornsakchai ◽

...

Keyword(s):

Candida Albicans ◽

Physical Properties ◽

Base Material ◽

Acrylic Resin ◽

Nanocomposite Films ◽

Denture Base

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Efektifitas Tablet Effervescent Ekstrak Kulit Manggis (Garcinia mangostana L) sebagai Pembersih Gigi Tiruan Resin Akrilik terhadap Candida albicans

STOMATOGNATIC - Jurnal Kedokteran Gigi ◽

10.19184/stoma.v16i2.23088 ◽

2019 ◽

Vol 16 (2) ◽

pp. 33

Author(s):

Jovanna Andhara Arieputri ◽

Dewi Kristiana ◽

R. Rahardyan Parnaadji

Keyword(s):

Candida Albicans ◽

Oral Mucosa ◽

Acrylic Resin ◽

Test Tube ◽

Plate Surface ◽

Garcinia Mangostana ◽

Denture Base ◽

Denture Stomatitis ◽

Mangosteen Peel ◽

Peel Extract

Denture stomatitis is inflammation of the oral mucosa in contact with the denture base caused by the fungus Candida albicans. Denture stomatitis can be prevented by cleaning the dentures either mechanically or chemically. Mangosteen peel extract has been shown to inhibit the growth of C. albicans, but its effectiveness as a denture cleaning agent needs to be improved. One way to increase effectiveness is by combining chemical cleansing and mechanical cleansing through effervescent tablets. The aim of the study was to determine the effectiveness of mangosteen peel extract effervescent tablets as an acrylic denture cleaner against C. albicans. Previously, the effervescent tablets of mangosteen peel extract were made with concentrations of 30% and 60%. Heat cured acrylic resin samples were incubated into a test tube containing C. albicans suspension for 24 hours at 37oC. Furthermore, the samples were immersed in effervescent tablets of mangosteen peel extract for 15 and 30 minutes. The number of C. albicans cells was counted using a spectrophotometer. The results showed that there was a significant reduction in the number of C. albicans cells attached to the plate surface after immersion in 30% and 60% effervescent tablets of mangosteen peel extract (p <0.05). Mangosteen peel extract effervescent tablets 60% more decreased the number of C. albicans cells. The duration of immersion did not show significantly different results (p> 0.05). Effervescent tablets of 60% concentration of mangosteen peel extract soaked for 15 minutes were the most effective as an acrylic resin denture cleaner against C. albicans.

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Disinfection Effect of 10% Ricinus Communis Oil on Candida Albicans Counts of Heat Polymerized Acrylic Resin

Dentika Dental Journal ◽

10.32734/dentika.v24i1.5615 ◽

2021 ◽

Vol 24 (1) ◽

pp. 6-10

Author(s):

Putri Welda Utami ◽

Nurdiana

Keyword(s):

Candida Albicans ◽

Ricinus Communis ◽

Base Material ◽

Acrylic Resin ◽

Biological Properties ◽

Chlorhexidine Gluconate ◽

P Value ◽

Denture Base ◽

Post Test ◽

Experimental Laboratory

Dentures is used to replace tooth loss. The most used denture base material is acrylic resin. The biological properties of acrylic resins give microorganisms the ability to colonize. Certain type of microorganism often found at the dentures base is Candida albicans. Denture’s disinfection is usually used to reduce Candida albicans. One of natural source that now is being widely researched is Ricinus communis oil. The purpose of this study was to analyze disinfection effect of Ricinus communis oil 10% on Candida albicans counts on heat polymerized acrylic resin. This research is an experimental laboratory study with a post-test only design. Samples was heat polymerization acrylic resin plates measuring 10 x 10 x 1 mm were made in the Department of Prosthodontic Faculty of Dentistry, Universitas Sumatera Utara with 10 plates for each for 10% Ricinus communis oil group and 0.2% chlorhexidine gluconate group (control). The Candida albicans colonies count was carried out in the Microbiology Laboratory, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara using colony counter. The data obtained was statistically count with the Kruskal-Wallis Test. The result showed the decrease in Candida albicans count with the highest value in the 10% Ricinus communis oil group is 9 x 100 CFU/ml and the highest value in the 0.2% chlorhexidine gluconate group is 0 x 100 CFU/ml. The Kruskal-Wallis test showed significant results with p value of 0.0001 (p <0.05) indicating there was a disinfection effect of 10% Ricinus communis oil and 0.2% chlorhexidine gluconate on Candida albicans count of heat polymerized acrylic resin. The study concluded that 10% Ricinus communis oil effective at decreasing Candida albicans count.

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Effect of Phosphoric Containing and Varnish-Coated Groups on Candida Albicans Adhesion and Porosity of Heat Cure Acrylic Denture Base Material

Biomedical & Pharmacology Journal ◽

10.13005/bpj/1360 ◽

2018 ◽

Vol 11 (1) ◽

pp. 179-185 ◽

Cited By ~ 1

Author(s):

Zahraa S. Abed Karkosh ◽

Basima M. A. Hussein ◽

Wifaq M. Ali AL-Wattar

Keyword(s):

Candida Albicans ◽

Polymethyl Methacrylate ◽

Base Material ◽

Acrylic Resin ◽

Denture Base ◽

Heat Cure ◽

Varnish Layer ◽

Varnish Coating ◽

Methacrylate Monomer ◽

Experimental Group

The aim of this study was to evaluate the effect of modified heat cured polymethyl methacrylate denture base (PMMA) or varnish coating of the denture base on candida albicans adhesion and porosity. First experimental group was prepared by addition of 15% phosphoric acid 2-hydroxyethyl methacrylate ester with polymethyl methacrylate monomer. While second experimental group prepared by varnish layer coating after material processing, these groups were compared with the control one. Three groups of vertex acrylic resin used as control, mPMMA and varnish coated group (PPH Cerkamed Wojciech Pawlowski, Poland). The inverted light microscope was used to measure the candida albicans adherence after one week and one month of processing. Two-way ANOVA, LSD test, T- test and Dunnett T3 were performed on data. Modified Acrylic resin showed highly significant reduction in candida albicans adhesion and porosity, also for varnish coating group showed highly significant reduction in candida albicans adhesion and porosity test.

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