scholarly journals Evaluation of the antibacterial activity of Borassus flabellifer male inflorescence against selected human pathogenic bacteria

2021 ◽  
Vol 11 (0) ◽  
pp. 3
Author(s):  
S. Vinujan ◽  
R. Jalani ◽  
S. Srivijeindran
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Human Pathogenic Bacteria ◽  
Male Inflorescence

scholarly journals TO STUDY THE ANTIBACTERIAL ACTIVITY OF VARIOUS EXTRACTS OF CLAUSENA DENTATA (WILLD.) ROEM.

2019 ◽  
pp. 69-73
Author(s):  
ANNAMALAI MADURAM ◽  
RAJU KAMARAJ
Keyword(s):  
Antibacterial Activity ◽  
Tamil Nadu ◽  
Pathogenic Bacteria ◽  
Volatile Oil ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Chloroform Extract ◽  
Klebsiella Pneumonia ◽  
Human Pathogens ◽  
Human Pathogenic Bacteria

Objectives: The objectives of the study were to study the antibacterial activity for the various extracts of Clausena dentata against human pathogens. Clausena (Rutaceae) is a genus of about 23 species of unarmed trees and shrubs. The stem bark of C. dentata is used in veterinary medicine for the treatment of wounds and sprains. Even though C. dentata has a lot of potential medical uses, the study of microbiological properties is very scarce. Methods: The plant C. dentata was collected from Kadagaman, near Tiruvannamalai, Tamil Nadu, India, and authenticated by Centre for Advanced Study in Botany, University of Madras, Chennai. The dry powder of stem bark was extracted with hexane, chloroform, and methanol. The extracts were subjected to qualitative phytochemical screening and antibacterial activity against human pathogenic bacteria such as Escherichia coli, Salmonella Typhi, Klebsiella pneumonia, Vibrio cholerae, and Staphylococcus aureus and compared with ciprofloxacin. Results: Qualitative chemical tests revealed the presence of various phytochemicals such as alkaloids, glycosides, carbohydrate, proteins and amino acids, phytosterols, and volatile oil. The antibacterial activity result reveals that all the extracts were are more active against V. cholerae. The activity against Pseudomonas aeruginosa was mild. Conclusion: The activity against V. cholerae was comparable with that of 5 μg/mL ciprofloxacin at the concentration of C. dentata 40 μg/mL. The orders of antibacterial activity against human pathogenic bacteria are hexane, methanol, and chloroform extract of C. dentata.

scholarly journals DELONIX REGIA BOJ. EX. HOOK RAFFIN; FAMILY: FABACEAE, BARK METHANOL EXTRACT PHYTOCHEMICAL PROFILE AND POTENTIAL THERAPEUTIC EVALUATION

2020 ◽  
pp. 100-105
Author(s):  
ARPITHA SHIVAMALLU ◽  
SHAILASREE SEKHAR
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Laser Scanning Microscopy ◽  
Charge Ratio ◽  
Delonix Regia ◽  
Human Pathogenic Bacteria ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Mcf 7

Objectives: The aim of this study was to evaluate the antioxidant, anti-inflammatory, and anti-cancer potencies of the Delonix regia bark, a first of its kind. Methods: The bark was extracted sequentially in Soxhlet apparatus with hexane, chloroform, and methanol in the increasing order of polarity. These extracts were subjected to find its antioxidant activity and total phenol content. Antibacterial activity against human pathogenic bacteria was tested. The anti-inflammatory properties were elucidated by its capacity to inhibit 15-lipoxygenase (LOX) and human cyclooxygenase (COX)-2. Cell cytotoxic capacity was evaluated against MCF-7 cells breast cancer cell lines. Results: Liquid chromatography (LC)-Mass Spectroscopy (MS) fingerprint of the methanol extract identified a total of 14 polyphenols, of which five were structurally characterized based on their mass-charge ratio [M-H]− peak, UV-vis absorption in comparison to published data. Antibacterial activity by disk diffusion inhibited human pathogenic bacteria. Bacterial biofilm inhibition capacity of extract (750 mg) imaged by confocal laser scanning microscopy revealed loss of microcolonies. Extract when tested for 15-LOX inhibition exhibited IC50 values of 94.5 ± 1.23 mg.mL−1 by enzyme kinetics studies using spectrophotometric techniques. Similarly, it could inhibit COX-2 enzyme at relatively lower concentrations (32.18 ± 1.91 mg.mL−1). Further, it quenched free radicals produced by Fentons’ reagent studied by DNS-nicking assay indicating its strong antioxidant property with the capacity to protect DNA. In vitro cytotoxicity was evaluated by 3-(4,5-dimethylthylthiazol-2-yl)-2,5-diphynyl tetrazolium bromide assay and apoptosis induced in MCF-7 cells was assessed morphologically. Conclusion: Our data suggest that D. regia bark methanol extract exerts its therapeutic activity for further pharmaceutical evaluations. Further studies are necessary to determine the mechanisms of these pharmacological properties.

scholarly journals ANTIBACTERIAL ACTIVITY OF ROBUSTA COFFEE (Coffea robusta L.) PEEL EXTRACT AGAINST HUMAN PATHOGENIC BACTERIA

2021 ◽  
Vol 9 (Spl-2-ICOPMES_2020) ◽  
pp. S264-S268
Author(s):  
Herlina Rante ◽  
◽  
Subehan . ◽  
Retno Wulandari ◽  
Yayu Mulsiani Evary ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Infectious Diseases ◽  
Pathogenic Bacteria ◽  
Fruit Peel ◽  
E Coli ◽  
Robusta Coffee ◽  
Human Pathogenic Bacteria ◽  
Tlc Bioautography ◽  
Coffea Robusta ◽  
Peel Extract

Now in these days infectious diseases seriously affect human health and sometimes these infections might become the cause of human mortality. Most of these infectious diseases are caused by bacteria, viruses, and fungi. Although large numbers of antibiotics are available increasing drug resistance in these microorganisms became a serious matter of concern in the scientific community. There is an urgent need for research on alternate natural products that can manage these pathogenic microorganisms without inducing any resistance. The purpose of this study was to determine the antibacterial activity of Robusta coffee (Coffea robusta L.) fruit peel extract against 5 human pathogenic bacteria i.e. Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Salmonella thypi NCTC 786. The sample was extracted using the maceration method with methanol as the solvent. The antibacterial activity of fruit peel extract was determined by using the agar diffusion method while the presence of active ingredients was determined by the using TLC-Bioautography assay performed using the mobile phase of n-hexane: ethyl acetate (1 : 3). The results of the study revealed significant antibacterial activity of coffee peel extract against E. coli and B. subtilis with an inhibition zone of 10.15 mm and 10.96 mm, respectively. Furthermore, results of the TLC-Bioautography revealed that the compounds at Rf 0.76 inhibit the growth of E. coli and the compounds at Rf 0.27 inhibit the growth of B. subtilis bacteria. These active spots were suspected to be flavonoid and phenolic compounds, respectively but further confirmation detail study is required in the future.

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