ARTIFICIAL DIETS FOR THE ADULT PARASITOID ITOPLECTIS CONQUISITOR (HYMENOPTERA: ICHNEUMONIDAE)

1980 ◽  
Vol 112 (3) ◽  
pp. 315-320 ◽  
Author(s):  
H. L. House
Keyword(s):  
Galleria Mellonella ◽  
Liquid Diet ◽  
The Other ◽  
Host Material ◽  
Natural Food ◽  
Artificial Diets ◽  
Adult Parasitoid ◽  
Emergence Rate ◽  
Host Pupa

AbstractTwo artificial diets — one liquid, the other candied — developed for adult Itoplectis conquisitor, an ichneumonid parasitoid, proved equal in performance for fecundity to a natural food regimen involving blood of host pupa, Galleria mellonella. Feeding on the liquid diet resulted in a parasitoid emergence rate from host pupae of 42.9 ± 8.6%; on the candy diet, 52.2 ± 15.8%; and on the natural regimen, 54.4 ± 4.0%. Therefore, host material was not needed to feed the adults. Other advantages of these artificial diets include convenience, economy, efficacy, and manageability for proper insect husbandry.

Chemical Defense of Lupins. Mollusc-Repellent Properties of Quinolizidine Alkaloids

1984 ◽  
Vol 39 (6) ◽  
pp. 553-558 ◽  
Author(s):  
Michael Wink
Keyword(s):  
Natural Products ◽  
Lactuca Sativa ◽  
Helix Pomatia ◽  
Free Food ◽  
The Other ◽  
Quinolizidine Alkaloids ◽  
Feeding Response ◽  
Artificial Diets ◽  
Arion Rufus

Polyphagous molluscs such as Helix pomatia and Arion rufus generally do not feed on plants containing alkaloids. Of 19 species tested 10 species were totally avoided, the other 9 species were less attacked than Lactuca sativa, which was readily taken. Plants containing quinolizidine alkaloids were studied in detail. Those species with the α-pyridone alkaloids cytisine and N-methylcytisine were avoided to a higher extent than plants with lupanine as the major alkaloid. Since the repellency observed could be due to other natural products present in the plants besides the alkaloids, the feeding response of Helix pomatia was tested on artificial diets containing quinolizidine alkaloids in various concentrations as the only variable. If the snails had the choice they clearly preferred alkaloid-free food or a diet with only low alkaloid concen­trations. Half-maximal repellency of cytisine is less than 2 mᴍ, of sparteine 1-5 mᴍ, and of lupanine 1-8mᴍ. Since the in vivo concentrations of sparteine, cytisine, and lupanine are equal to or higher than the inhibitory concentrations required it is concluded that quinolizidine alkaloids constitute a potential antimolluscan principle of legumes.

scholarly journals In Vitro and In Vivo Activity of Single and Dual Antimicrobial Agents Against KPC-producing Klebsiella pneumoniae

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S379-S379
Author(s):  
Farzad Moussavi ◽  
Sarath Nath ◽  
Daniel Abraham ◽  
David Landman ◽  
John Quale
Keyword(s):  
Antimicrobial Agents ◽  
Galleria Mellonella ◽  
Survival Rates ◽  
Polymyxin B ◽  
The Other ◽  
In Vivo Model ◽  
Serious Infections ◽  
Time Kill

Abstract Background Options for treatment of infections due to KPC-producing K. pneumoniae are limited, and combination therapy is often recommended. In this report, the in vitro and in vivo activity of potential therapeutic agents and combinations was assessed against four KPC-producing K. pneumoniae isolates. Methods Using clinically-relevant concentrations, time-kill experiments and the Galleria mellonella model of infection were used to examine the activity of polymyxin B, ceftazidime-avibactam, meropenem, rifampin, and amikacin alone and in combination. Four isolates of KPC-producing K. pneumoniae were studied, including two isolates that were resistant to polymyxin B and had ceftazidime-avibactam MICs of 8 µg/mL. The other two K. pneumoniae isolates were susceptible to polymyxin B and had lower MICs of ceftazidime-avibactam. Results Two isolates that were resistant to polymyxin B and with ceftazidime-avibactam MICs of 8 µg/mL were also resistant to amikacin and meropenem. When ceftazidime-avibactam was combined with either amikacin or meropenem, synergy was observed in vitro, and these combinations were associated with improved survival with the in vivo model. The other two K. pneumoniae isolates were susceptible to polymyxin B and had lower MICs of ceftazidime-avibactam. At concentrations four times the MIC, ceftazidime-avibactam had bactericidal activity in vitro; at one fourth the MIC, synergy was observed when combined with meropenem. Improved survival rates were observed with therapy with ceftazidime-avibactam, particularly when combined with a second agent for one isolate. In the in vivo model, polymyxin B with or without rifampin or meropenem, was ineffective against polymyxin B resistant strains. Conclusion Pending clinical studies, combining ceftazidime-avibactam with another agent (e.g., a carbapenem) should be encouraged when treating serious infections due to these pathogens, especially for isolates with ceftazidime-avibactam MICs near the susceptibility breakpoint. Disclosures All authors: No reported disclosures.

scholarly journals EMERGENCE RATE OF THE MEXICAN BEAN WEEVIL IN VARIETIES OF BEANS FROM THE SOUTHWESTERN AMAZON

2018 ◽  
Vol 31 (4) ◽  
pp. 1048-1053
Author(s):  
Lucas Martins Lopes ◽  
Josiane Moura do Nascimento ◽  
Vanderley Borges Dos Santos ◽  
Lêda Rita Dantonino Faroni ◽  
Adalberto Hipólito de Sousa
Keyword(s):  
The Other ◽  
Common Beans ◽  
Insect Development ◽  
Bean Weevil ◽  
Phaseolus Vulgaris L ◽  
Insect Emergence ◽  
Emergence Period ◽  
Plant Variety ◽  
Adult Insect ◽  
Emergence Rate

ABSTRACT Common beans (Phaseolus vulgaris L.) are one of the most important protein sources worldwide. However, infestation by bruchids compromises the storage of these beans after harvest. The objective of this study was to determine the emergence rate of Zabrotes subfasciatus (Coleoptera: Chrysomelidae) in four P. vulgaris varieties (Carioca Pitoco, Enxofre, Gorgutuba Vermelho, and Rosinha) cultivated in the southwestern Amazon (State of Acre, Brazil). The grains from each variety were infested with 50 non-sexed adult Z. subfasciatus, and the insects were collected 13 days after initiating the bioassays. The adult progeny was collected from each plant variety on alternate days from the beginning to the end of the emergence period, and they were counted (number of insects/jar). The sum of emerged insects per day (SEd) was determined from the beginning of the emergence period, from which the accumulated emergence was calculated (SEa= ΣSEd, % day). There were differences in the emergence rates of Z. subfasciatus among the bean varieties, with the rates being lower in the Gorgutuba Vermelho variety. Although this bean variety had the lowest peak of adult insect emergence, the period of insect development was not of increased length in this variety compared to the other varieties. In general, the Gorgutuba Vermelho variety appears to be resistant to Z. subfasciatus.

Development of Cotesia orobenae Forbes (Hymenoptera: Braconidae) in its Host, Evergestis rimosalis (Guenée) (Lepidoptera: Pyralidae)

2001 ◽  
Vol 36 (1) ◽  
pp. 9-16
Author(s):  
L. T. Kok ◽  
J. A. Acosta-Martinez
Keyword(s):  
Sex Ratios ◽  
Host Density ◽  
Female Offspring ◽  
Developmental Time ◽  
The Other ◽  
Optimal Temperature ◽  
Density Effects ◽  
Adult Parasitoid ◽  
Early Instar ◽  
Lepidoptera Pyralidae

Temperature and host density effects on the development of Cotesia orobenae Forbes (Hymenoptera: Braconidae), and its parasitism of the cross-striped cabbageworm, Evergestis rimosalis (Guenée) (Lepidoptera: Pyralidae), were investigated. Time for adult parasitoid emergence from cocoon masses of C. orobenae maintained at 15, 20, 25, 30 and 35°C and a photoperiod of 15:9 (L: D) was inversely related to temperature. Parasitoid emergence exceeded 86% for all temperatures except that at 35°C. Development of C. orobenae on early-instar hosts was best at 20 and 25°C. Although the shortest developmental time was observed at 35°C, survival of both parasitoid and host was low at this temperature. At 15°C, the parasitoid was not very active, and developmental time was the longest among the temperatures tested. Percent of host larvae parasitized was significantly higher at 20 and 25°C than the other temperatures tested. Females were short-lived and oviposited an average of 118 and 136 eggs at 20 and 25°C, respectively. Females died after 24 h of being placed in the oviposition chamber at 30 and 35°C. At 15°C they lived longer, but did not parasitize host larvae provided. Virgin females produced only male progeny. Mated females produced slightly more female offspring than males. Sex ratios of progeny from females paired with single or multiple males were not different. Percentage parasitization of host larvae between treatments differed for the first day, but not the second day. Fecundity of C. orobenae exposed to five host densities (5, 10, 15, 20, and 25 per parasitoid) showed significant differences in percent parasitization among the treatments. Percentage parasitization was highest with five and 10 hosts per parasitoid. The highest number of parasitoid cocoons recovered per cocoon mass was obtained with 10 hosts. The data indicate that optimal temperature for C. orobenae oviposition, development, and parasitism of its host is between 20 to 25°C.

scholarly journals Gestational obesity accentuates obesity in obesity-prone progeny

1998 ◽  
Vol 275 (4) ◽  
pp. R1374-R1379 ◽  
Author(s):  
Barry E. Levin ◽  
Elizabeth Govek
Keyword(s):  
Genetic Background ◽  
Maternal Obesity ◽  
High Energy ◽  
Liquid Diet ◽  
The Other ◽  
Glucose Levels ◽  
Fat Depots ◽  
Diet Induced Obesity ◽  
Obesity And Diabetes ◽  
Fat Pads

Maternal obesity and genetic background can affect the development of obesity and diabetes in offspring. Here we used selected strains of rats resistant (DR) vs. susceptible to development of diet-induced obesity (DIO) on high-energy (HE) diets to assess this issue. DR and DIO dams were fed either Chow or HE diet for 4 wk. DIO HE diet-fed dams and additional DR rats fed a palatable liquid diet (Ensure) became more obese and hyperinsulinemic than the other groups. During lactation, all dams were fed their respective diets, and offspring were fed Chow from weaning to 16 wk of age. All offspring of DIO dams gained more weight and had heavier retroperitoneal fat pads and higher leptin levels than DR progeny, but offspring of the more obese DIO HE dams had heavier fat pads and higher glucose levels than DIO Chow offspring. After 4 wk on HE diet, all DIO offspring gained more weight and had heavier total adipose depots and higher insulin and leptin levels than DR offspring. Offspring of DIO HE dams also gained more weight and had heavier fat depots and higher leptin levels than DIO Chow offspring. Therefore maternal obesity and hyperinsulinemia were associated with increased obesity in those offspring already genetically predisposed to become obese.

scholarly journals Aspects of the biology and behavior of Nesolynx sp. (Hymenoptera, Eulophidae)

1987 ◽  
Vol 44 (1) ◽  
pp. 105-117
Author(s):  
Vanda Helena Paes Bueno ◽  
Evoneo Berti-Filho ◽  
José Claret Matioli
Keyword(s):  
Life Cycle ◽  
Host Species ◽  
Galleria Mellonella ◽  
Dorsal Region ◽  
Considerable Decrease ◽  
Host Pupa ◽  
Pupal Parasite ◽  
Parthenogenetic Reproduction ◽  
Increasing Temperature ◽  
And Behavior

This research was carried out to study some aspects of the biology and behavior of Nesolynx sp. (Hymenoptera, Eulophidae), a pupal parasite of Psorocampa denticulata (Lepidoptera, Notodontidae) a defoliating caterpillar of Eucalyptus spp. in Brazil. The adults emerge from the host pupa through a circular hole on Its dorsal region. Mating occurs righ after the emergence and the longevity of adults was two days for the males and four days for the females. Regarding to the host species Diatraea saccharalis showed a number of adults significantly greater than Galleria mellonella and the increasing temperature from 21±1 °C to 26±1°C caused a significative increasing in the number of emerged adults in both host species. The emergence of adults increased proportionally to the period of exposition to the host up to 3.50 days; after that, a considerable decrease in the emergence was observed. The parasitoid showed parthenogenetic reproduction therefore the average number of emerged males was significantly greater than the number of females. The sex ratio was similar for the insects emerged from virgin or mated females (0,96) and the life cycle lenght was around 18.34 days for both conditions.

Notes on the distribution and biology of Pediobius furvus (Gah.) (Hym., Eulophidae), a parasite of graminaceous stem-borers

1970 ◽  
Vol 59 (4) ◽  
pp. 681-689 ◽  
Author(s):  
A. I. Mohyuddin
Keyword(s):  
Biological Control ◽  
Life Cycle ◽  
Sesamia Calamistis ◽  
The Other ◽  
Stem Borers ◽  
Single Host ◽  
Host Pupa ◽  
Dentichasmias Busseolae ◽  
Apanteles Sesamiae

Pediobius furvus (Gah.) (Eulophidae) is a parasite of the pupae of Pyralid and Noctuid stem-borers on graminaceous crops in Africa between 17°N and 17°S. It co-exists with the other pupal parasites Dentichasmias busseolae Heinr. and Procerochasmias nigromaculatus (Cam.) (Ichneumonidae) and Hyperchalcidia soudanensis Steffan (Chalcididae), but it is more abundant below 4000 ft where P. nigromaculatus does not occur. Mating follows a courtship dance, and oviposition occurs in any part of the host pupa. The life-cycle is completed in 18–20 days at 30°C, and up to several hundred adults may emerge from a single host pupa, the most suitable pupae being 2–3 days old at the time of oviposition. Longevity was improved when sucrose was provided as food. Host pupae outside the stem were parasitised, but puparia of Diptera and cocoons of Apanteles sesamiae Cam. (Braconidae) were not parasitised in the laboratory. Pupae formed inside cocoons were not attacked. Pediobius furvus has become established in Madagascar on Sesamia calamistis Hmps, and is considered promising for biological control of graminaceous stem-borers in other countries.

EMERGENCE, SEEDLING GROWTH, AND YIELD OF ALTAI WILD RYEGRASS AND OTHER GRASSES AS INFLUENCED BY SOIL TEMPERATURE AND FERTILITY

1972 ◽  
Vol 52 (5) ◽  
pp. 795-800
Author(s):  
T. LAWRENCE ◽  
M. R. KILCHER
Keyword(s):  
Soil Temperature ◽  
Bromus Inermis ◽  
The Other ◽  
Growth And Yield ◽  
Young Plant ◽  
Agropyron Elongatum ◽  
Rate Of Development ◽  
Tall Wheatgrass ◽  
Emergence Rate ◽  
Plant Yields

Altai wild ryegrass (Elymus angustus Trin.) was compared to Russian wild ryegrass (Elymus junceus Fisch.), bromegrass (Bromus inermis Leyss.), tall wheatgrass (Agropyron elongatum Host.), and intermediate wheat-grass (Agropyron intermedium (Host.) Beauv.) when seeded in soils maintained at 10, 15, and 20 C and fertilized at various rates of N and P. Emergence, rate of development, and young plant yields were measured. Altai wild ryegrass emergence was retarded by cold soil more so than that of the other grasses. Its response to warmer soil was proportionately greater than that of other grasses. Like Russian wild ryegrass, Altai wild ryegrass showed little response to N fertilizers when grown in cold soils, except when P was added.

scholarly journals Candidiasis by Candida glabrata, Candida nivariensis and Candida bracarensis in Galleria mellonella: Virulence and Therapeutic Responses to Echinocandins

2021 ◽  
Vol 7 (12) ◽  
pp. 998
Author(s):  
Ainara Hernando-Ortiz ◽  
Elena Eraso ◽  
Guillermo Quindós ◽  
Estibaliz Mateo
Keyword(s):  
Candida Glabrata ◽  
Therapeutic Response ◽  
Galleria Mellonella ◽  
The Other ◽  
Closely Related Species ◽  
Lethal Infection ◽  
Candida Bracarensis ◽  
Candida Nivariensis ◽  
Therapeutic Responses

Candida albicans is the major etiological agent of invasive candidiasis but the increasing prevalence of emerging species of Candida, such as Candida glabrata and phylogenetically closely related species, Candida nivariensis and Candida bracarensis, requires special attention. Differences in virulence among these species and their therapeutic responses using in vivo non-mammalian models are scarcely analysed. The aim of this study was analyse the survival of G. mellonella and host-pathogen interactions during infection by C. glabrata, C. nivariensis and C. bracarensis. Moreover, therapeutic responses to echinocandins were also assessed in the G. mellonella model of candidiasis. These three species produced lethal infection in G. mellonella; C. glabrata was the most virulent species and C. bracarensis the less. Haemocytes of G. mellonella phagocytised C. bracarensis cells more effectively than those of the other two species. Treatment with caspofungin and micafungin was most effective to protect larvae during C. glabrata and C. nivariensis infections while anidulafungin was during C. bracarensis infection. The model of candidiasis in G. mellonella is simple and appropriate to assess the virulence and therapeutic response of these emerging Candida species. Moreover, it successfully allows for detecting differences in the immune system of the host depending on the virulence of pathogens.

scholarly journals PERKEMBANGAN DAN REPRODUKSI CROCIDOLOMIA PAVONANA (F.) (LEPIDOPTERA: PYRALIDAE) PADA PAKAN ALAMI DAN SEMIBUATAN

2004 ◽  
Vol 4 (2) ◽  
pp. 53-61
Author(s):  
Novalia Jelita Sari ◽  
Djoko Prijono
Keyword(s):  
Emerging Adults ◽  
Chinese Cabbage ◽  
Larval Instar ◽  
The Other ◽  
Pupal Weight ◽  
Artificial Diets ◽  
Adult Lifespan ◽  
Pupal Period ◽  
Microbial Inhibitors ◽  
Lepidoptera Pyralidae

Development and reproduction of Crocidolomia pavonana (F.) (Lepidoptera: Pyralidae) on natural and artificial diets.  Development and reproduction of Crocidolomia pavonana on natural and artificial diets were studied in the laboratory (25.2 ± 0.9 C; 84.6% ± 6.7% RH; ca. 12 h photophase).  The natural diets tested were broccoli, cabbage, chinese cabbage, and cauliflower leaves.  The artificial diets used were agar–based general lepidopteran diets mixed with red bean or broccoli leaves, and supplemented with vitamins and microbial inhibitors.  Observation of insect development was done since the egg stage.  After the eggs hatched, larvae were kept singly in plastic cups and fed with appropriate diets.  Fifty larvae were used for each diet.  Records were kept with regard to the duration of each larval instar, pupal period, and the pupal weight.  The emerging adults were paired, and then the number of dead adults and that of eggs laid were recorded daily.  On all the natural diets tested, the larval stage of C. pavonana passed through four instars.  The egg incubation, total larval developmental, and pupal period of C. pavonana on chinese cabbage were the shortest compared to those on the other natural diets.  The pupal weight, however, was the highest on broccoli, followed by that on chinese cabbage, cabbage, and cauliflower.  The female fecundity was also the highest on broccoli (average 258 eggs/female) followed by that on chinese cabbage (212), cauliflower (162), and cabbage (102).  Broccoli diet also yielded adults with the longest lifespan although the adult lifespan on broccoli was not significantly different from that on the other natural diets, except that of males on cabbage.  C. pavonana failed to develop successfully on six kinds of artificial diets tested.  The best artificial diet (broccoli–based diet with microbial inhibitors 20% of the normal amount) only yielded two males and five females with deformed wings, but none of the emerging females produced eggs.

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