scholarly journals PERKEMBANGAN DAN REPRODUKSI CROCIDOLOMIA PAVONANA (F.) (LEPIDOPTERA: PYRALIDAE) PADA PAKAN ALAMI DAN SEMIBUATAN

2004 ◽  
Vol 4 (2) ◽  
pp. 53-61
Author(s):  
Novalia Jelita Sari ◽  
Djoko Prijono
Keyword(s):  
Emerging Adults ◽  
Chinese Cabbage ◽  
Larval Instar ◽  
The Other ◽  
Pupal Weight ◽  
Artificial Diets ◽  
Adult Lifespan ◽  
Pupal Period ◽  
Microbial Inhibitors ◽  
Lepidoptera Pyralidae

Development and reproduction of Crocidolomia pavonana (F.) (Lepidoptera: Pyralidae) on natural and artificial diets.  Development and reproduction of Crocidolomia pavonana on natural and artificial diets were studied in the laboratory (25.2 ± 0.9 C; 84.6% ± 6.7% RH; ca. 12 h photophase).  The natural diets tested were broccoli, cabbage, chinese cabbage, and cauliflower leaves.  The artificial diets used were agar–based general lepidopteran diets mixed with red bean or broccoli leaves, and supplemented with vitamins and microbial inhibitors.  Observation of insect development was done since the egg stage.  After the eggs hatched, larvae were kept singly in plastic cups and fed with appropriate diets.  Fifty larvae were used for each diet.  Records were kept with regard to the duration of each larval instar, pupal period, and the pupal weight.  The emerging adults were paired, and then the number of dead adults and that of eggs laid were recorded daily.  On all the natural diets tested, the larval stage of C. pavonana passed through four instars.  The egg incubation, total larval developmental, and pupal period of C. pavonana on chinese cabbage were the shortest compared to those on the other natural diets.  The pupal weight, however, was the highest on broccoli, followed by that on chinese cabbage, cabbage, and cauliflower.  The female fecundity was also the highest on broccoli (average 258 eggs/female) followed by that on chinese cabbage (212), cauliflower (162), and cabbage (102).  Broccoli diet also yielded adults with the longest lifespan although the adult lifespan on broccoli was not significantly different from that on the other natural diets, except that of males on cabbage.  C. pavonana failed to develop successfully on six kinds of artificial diets tested.  The best artificial diet (broccoli–based diet with microbial inhibitors 20% of the normal amount) only yielded two males and five females with deformed wings, but none of the emerging females produced eggs.

scholarly journals Comparative Biology of Pink Bollworm, Pectinophora gossypiella Saund. on Bt. and Non-Bt. Cotton

2019 ◽  
Vol 62 (2) ◽  
pp. 116-121
Author(s):  
Imran Ali Rajput ◽  
Abdul Mubeen Lodhi ◽  
Tajwer Sultana Syed ◽  
Ghulam Hussain Abro ◽  
Imran Khatri
Keyword(s):  
Larval Instar ◽  
Pink Bollworm ◽  
Pectinophora Gossypiella ◽  
Pupal Weight ◽  
Bt Cotton ◽  
Larval Duration ◽  
Biological Parameters ◽  
Total Period ◽  
Pupal Period ◽  
Mortality Percentage

Effect of Bt. and non-Bt. cotton variety was investigated on biological parameters of pink bollworm Pectinophora gossypiella. In biological parameters, maximum larval duration of 4.5±0.07 days were recorded in the first instar on non-Bt. cotton and minimum 2.00±0.06 days in fourth larval instar on Bt. cotton. The larval weight was higher (20.24±1.74 mg) on non-Bt. and minimum on Bt. cotton (13.84±1.34 mg); meanwhile the maximum pupal weight of 23.46±0.55 mg was weighed on non-Bt. cotton and minimum 17.41±0.44 mg on Bt. cotton. A total period of 18.44±0.55 day’s adult female lived on non-Bt. cotton and15.44±0.43 on Bt. cotton. The overall maximum mean numbers of 119±1.99 eggs were observed on non-Bt. cotton as compared to Bt. cotton. Similarly, ovipositional rate was also higher (8.04±0.19 eggs/ female/day) on non-Bt. cotton. The maximum larval duration (11.36±0.30 days) was higher on non-Bt. cotton and minimum on Bt. cotton (8.03±0.43 days); meanwhile the maximum pupal period of 9.73±0.29 days were calculated on Bt. cotton and almost similar days of pupal period on non-Bt. cotton crop, respectively. The highest mortality percentage of all stages was found on Bt. cotton with maximum mortality percentage of 24.59% at 1st larval instar.

Penetration and encapsulation of the larval endoparasitoid Exorista larvarum (Diptera: Tachinidae) in the factitious host Galleria mellonella (Lepidoptera: Pyralidae)

2013 ◽  
Vol 104 (2) ◽  
pp. 203-212 ◽  
Author(s):  
A. Valigurová ◽  
V. Michalková ◽  
P. Koník ◽  
M.L. Dindo ◽  
M. Gelnar ◽  
...  
Keyword(s):  
Galleria Mellonella ◽  
Larval Instar ◽  
Posterior Part ◽  
Immunological Response ◽  
Dark Spot ◽  
Foreign Objects ◽  
Factitious Host ◽  
Lepidoptera Pyralidae ◽  
Cellular Defence ◽  
Host Tissues

AbstractThe tachinid fly Exorista larvarum (L.) (Diptera: Tachinidae) is a polyphagous larval endoparasitoid that deposits its eggs on the host exoskeleton of lepidopteran and tenthredinid larvae. The attachment of larval E. larvarum and the formation of the respiratory funnel were studied during infestation in the last larval instar of the wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae). The tachinid larvae burrow through the host integument after hatching, using their robust cephalopharyngeal skeleton, leaving a dark spot at the point of their penetration as a result of host cuticle melanization. Endoparasitoid penetration induces the host cellular defence, resulting in the formation of a haemocyte capsule consisting of multi-cellular sheaths. This enveloping capsule later undergoes melanization, which is mostly obvious towards the posterior part of the endoparasitoid. The endoparasitoid uses the host encapsulation response to build a respiratory funnel from the modified host integument, leading to the host surface. The encapsulated larva remains attached to the respiratory funnel via an anal hook and cuticular spines until fully developed. Additional immunohistochemical analyses were used to study host–parasitoid interactions. Indirect immunofluorescence showed no labelling of potential tachinid antigens and confirmed no effect on the surrounding host tissues. A simulated parasitization with coated polybead microspheres revealed the mortal impact of tachinid antigens to the host. Hosts injected with antigen-coated polybeads died as a consequence of an acute and extensive immunological response to the tachinid antigens and not due to the trauma caused by foreign objects inside their body.

scholarly journals Biology of the Wild SilkmothAnaphe panda(Boisduval) in the Kakamega Forest of Western Kenya

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
N. Mbahin ◽  
S. K. Raina ◽  
E. N. Kioko ◽  
J. M. Mueke
Keyword(s):  
Life Cycle ◽  
Incubation Period ◽  
Rainy Season ◽  
Dry Season ◽  
Developmental Period ◽  
The Other ◽  
Western Kenya ◽  
Kakamega Forest ◽  
Pupal Period ◽  
Indigenous Forest

A study on the life cycle of the silkmothAnaphe panda(Boisduval) was conducted in two different habitats of the Kakamega Forest in western Kenya: Ikuywa, an indigenous forest, and Isecheno, a mixed indigenous forest. Eggs were laid in clusters, and the incubation period ranged from 40 to 45 days. Larvae fed onBridelia micrantha(Hochst) and passed through seven instars. The developmental period took between 83 to 86 days in the dry season and 112 to118 days in the rainy season. The pupal period ranged between 158 and 178 days in the rainy season and, on the other hand, between 107 and 138 days in the dry season. But the later caught up in development with those that formed earlier. Moths emerged from mid-October until mid-May. Longevity of adultAnaphe pandamoths took between 4 and 6 days, but generally females seemed to live longer than males. The moth also seems to have higher lifespan in the indigenous forest compared to the mixed indigenous forest.

NEW DESCRIPTIONS OF LARVAE OF FOREST INSECTS: SEMIOTHISA, DYSMIGIA (LEPIDOPTERA, GEOMETRIDAE)

1947 ◽  
Vol 79 (6) ◽  
pp. 113-116 ◽  
Author(s):  
W. C. McGuffin
Keyword(s):  
Transition Period ◽  
Larval Instar ◽  
Larval Period ◽  
The Other ◽  
Forest Insects ◽  
Other Hand ◽  
Larval Moult

Some geometrid larvae exhibit dimorphism in colour. Several interesting observations of this phenomenon were made during the study of larvae of the species, Semiothisa sexmaculata Pack., Dysmigia loricaria Evers., and Nepytia canosaria Wlk.In these observations, certain points stand out. Although larvae of S. sexmaculata are always green until they reach the last instar, both brown and green phases occur in that stage. After the last larval moult, a transition period of approximately two days is required for larvae to acquire the colouring of the brown phase. On the other hand, specimens of D. loricaria may pass their entire larval period in either a brown or a green phase (as do the larvae of N. canosaria, descriptions of which may be found in Can. Ent. 75: 186-189) or they may change (as do some larvae of S. sexmaculata) from the green phase to the brown phase in the last larval instar. For Dysmigia larvae to undergo this transformation, approximately three days are required.

scholarly journals Quantitative and Qualitative Impacts of Selected Arthropod Venoms on the Larval Haemogram of the Greater Wax Moth, Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae)

2021 ◽  
pp. 1-26
Author(s):  
Karem Ghoneim ◽  
Khalid Hamadah ◽  
Mohammad Tanani ◽  
Dyaa Emam
Keyword(s):  
Apis Mellifera ◽  
Galleria Mellonella ◽  
Larval Instar ◽  
Population Count ◽  
Oriental Hornet ◽  
Greater Wax Moth ◽  
The World ◽  
Lepidoptera Pyralidae ◽  
Hemocyte Population ◽  
Wax Moth

The greater wax moth, Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae) is the most destructive pest of honey bee, Apis mellifera Linnaeus (Hymenoptera: Apidae), throughout the world. The present study was conducted to determine the quantitative and qualitative impairing effects of the arthropod venoms, viz., death stalker scorpion Leiurus quinquestriatus (Hemprich & Ehrenberg) venom (SV), oriental Hornet (wasp) Vespa orientalis Linnaeus venom (WV) and Apitoxin of A. mellifera (AP) on the larval haemogram. For this purpose, the 3rd instar larvae were treated with LC50 of each of these venoms (3428.9, 2412.6, and 956.16 ppm, respectively). The haematological investigation was conducted in haemolymph of the 5th and 7th (last) instar larvae. The important results could be summarized as follows. Five basic types of the freely circulating haemocytes in the haemolymph of last instar (7th) larvae of G. mellonella had been identified: Prohemocytes (PRs), Plasmatocytes (PLs), Granulocytes (GRs), Spherulocytes (SPs) and Oenocytoids (OEs). All venoms unexceptionally prohibited the larvae to produce normal hemocyte population (count). No certain trend of disturbance in the differential hemocyte counts of circulating hemocytes in larvae of G. mellonella after treatment with the arthropod venoms. Increasing or decreasing population of the circulating hemocytes seemed to depend on the potency of the venom, hemocyte type and the larval instar.  In PRs of last instar larvae, some cytopathological features had been observed after treatment with AP or WV, but SV failed to cause cytopathological features. With regard to PLs, some cytopathological features had been observed after treatment with AP while both SV and WV failed to cause cytopathological features in this hemocyte type. No venom exhibited cytopathological effects on GRs, SPs or OEs.

scholarly journals Analysis of Flavonoid Metabolites in Watercress (Nasturtium officinale R. Br.) and the Non-Heading Chinese Cabbage (Brassica rapa ssp. chinensis cv. Aijiaohuang) Using UHPLC-ESI-MS/MS

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5825
Author(s):  
Xiaoqing Ma ◽  
Qiang Ding ◽  
Xilin Hou ◽  
Xiong You
Keyword(s):  
Mass Spectrometry ◽  
Chinese Cabbage ◽  
High Performance ◽  
Reference Value ◽  
The Other ◽  
Tandem Mass ◽  
Nasturtium Officinale ◽  
Esi Ms ◽  
Heading Chinese Cabbage ◽  
Ionization Tandem Mass Spectrometry

Flavonoids from plants play an important role in our diet. Watercress is a special plant that is rich in flavonoids. In this study, four important watercress varieties were compared with non-heading Chinese cabbage by ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS). A total of 132 flavonoid metabolites (including 8 anthocyanins, 2 dihydroflavone, 3 dihydroflavonol, 1 flavanols, 22 flavones, 11 flavonoid carbonosides, 82 flavonols, and 3 isoflavones) were detected. Flavonoid metabolites varied widely in different samples. Both the non-heading Chinese cabbage and the variety of watercress from Guangdong, China, had their own unique metabolites. This work is helpful to better understand flavonoid metabolites between the non-heading Chinese cabbage and the other four watercress varieties, and to provide a reliable reference value for further research.

scholarly journals The efficiency of Paecilomyce farinosus as biocontrol to larve stage on second ad sixith instar larve of Trogoderma granarium (Everts)

2013 ◽  
Vol 10 (4) ◽  
pp. 1096-1101
Author(s):  
Baghdad Science Journal
Keyword(s):  
Larval Instar ◽  
The Other ◽  
Biocontrol Agents ◽  
Larval Instars ◽  
Paecilomyces Farinosus ◽  
Trogoderma Granarium ◽  
Laboratory Conditions ◽  
Other Hand ◽  
Laboratory Results ◽  
Treatment Concentration

The present study was conducted to determine the pathogenicity of Paecilomyces farinosus as biocontrol agents against Tragoderma granarium (khapra) under laboratory conditions with three concentrations ( 2.8 x 108 , 2.8 x 106 ,2.8 x104 spores/ ml). The laboratory results revealed that fungi showed it's higher pathogencity to larve stage on 2nd instar and 6th instar , although their capability differs according to the fungi concentrations this fungi caused higher mortality of 2nd larval instar 70% and 60% ,55% when it was used at concentrations 2 .8 x 106 ,2.8 x104 spores/ ml respectively. On the other hand on 6th larval instars 55% , 35% , 30% at concentrations ( 2.8 x 108 , 2.8 x 106 ,2.8 x104 spores/ ml) respectively, and the highest rate of distortions in the adult insects from the 2nd instar treatment than the adult insects from the 6th instar treatment concentration of this fungi.

scholarly journals Production of germ-free mosquitoes via transient colonisation allows stage-specific investigation of host-microbiota interactions

2019 ◽  
Author(s):  
Ottavia Romoli ◽  
Johan Claes Schönbeck ◽  
Siegfried Hapfelmeier ◽  
Mathilde Gendrin
Keyword(s):  
Larval Development ◽  
Emerging Adults ◽  
Larval Instar ◽  
Bacterial Colonisation ◽  
Germ Free ◽  
Functional Studies ◽  
The Third ◽  
Novel Approach ◽  
Folate Biosynthesis ◽  
Vector Borne

AbstractThe mosquito microbiota impacts the physiology of its host and is essential for normal larval development, thereby influencing transmission of vector-borne pathogens. Germ-free mosquitoes generated with current methods show larval stunting and developmental deficits. Therefore, functional studies of the mosquito microbiota have so far mostly been limited to antibiotic treatments of emerging adults. In this study, we developed a novel approach to produce germ-free Aedes aegypti mosquitoes. It is based on reversible colonisation with bacteria genetically modified to allow complete decolonisation at any developmental stage. We show that, unlike germ-free mosquitoes previously produced using sterile diets, reversibly colonised mosquitoes show no developmental retardation and reach the same size as control adults. This allowed us to uncouple the study of the microbiota in larvae and adults. In adults, we detected no impact of bacterial colonisation on mosquito fecundity or longevity. In larvae, we performed a transcriptome analysis and diet supplementation experiments following decolonisation during the third larval instar. Our data suggest that bacteria support larval development by contributing to folate biosynthesis and by enhancing energy storage. Our study establishes a novel tool to study the microbiota in insects and deepens our knowledge on the metabolic contribution of bacteria to mosquito development.

Chemical Defense of Lupins. Mollusc-Repellent Properties of Quinolizidine Alkaloids

1984 ◽  
Vol 39 (6) ◽  
pp. 553-558 ◽  
Author(s):  
Michael Wink
Keyword(s):  
Natural Products ◽  
Lactuca Sativa ◽  
Helix Pomatia ◽  
Free Food ◽  
The Other ◽  
Quinolizidine Alkaloids ◽  
Feeding Response ◽  
Artificial Diets ◽  
Arion Rufus

Polyphagous molluscs such as Helix pomatia and Arion rufus generally do not feed on plants containing alkaloids. Of 19 species tested 10 species were totally avoided, the other 9 species were less attacked than Lactuca sativa, which was readily taken. Plants containing quinolizidine alkaloids were studied in detail. Those species with the α-pyridone alkaloids cytisine and N-methylcytisine were avoided to a higher extent than plants with lupanine as the major alkaloid. Since the repellency observed could be due to other natural products present in the plants besides the alkaloids, the feeding response of Helix pomatia was tested on artificial diets containing quinolizidine alkaloids in various concentrations as the only variable. If the snails had the choice they clearly preferred alkaloid-free food or a diet with only low alkaloid concen­trations. Half-maximal repellency of cytisine is less than 2 mᴍ, of sparteine 1-5 mᴍ, and of lupanine 1-8mᴍ. Since the in vivo concentrations of sparteine, cytisine, and lupanine are equal to or higher than the inhibitory concentrations required it is concluded that quinolizidine alkaloids constitute a potential antimolluscan principle of legumes.

scholarly journals Well-Being and Romantic Relationships: A Systematic Review in Adolescence and Emerging Adulthood

2019 ◽  
Vol 16 (13) ◽  
pp. 2415 ◽  
Author(s):  
Mercedes Gómez-López ◽  
Carmen Viejo ◽  
Rosario Ortega-Ruiz
Keyword(s):  
Systematic Review ◽  
Romantic Relationships ◽  
Emerging Adulthood ◽  
Emerging Adults ◽  
Meta Analysis ◽  
Well Being ◽  
The Other ◽  
The One ◽  
Definition Of ◽  
The Relationship

Adolescence and emerging adulthood are both stages in which romantic relationships play a key role in development and can be a source of both well-being and negative outcomes. However, the limited number of studies prior to adulthood, along with the multiplicity of variables involved in the romantic context and the considerable ambiguity surrounding the construct of well-being, make it difficult to reach conclusions about the relationship between the two phenomena. This systematic review synthesizes the results produced into this topic over the last three decades. A total of 112 studies were included, following the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) guidelines. On the one hand, these works revealed the terminological heterogeneity in research on well-being and the way the absence of symptoms of illness are commonly used to measure it, while on the other hand, they also showed that romantic relationships can be an important source of well-being for both adolescents and emerging adults. The findings underline the importance of providing a better definition of well-being, as well as to attribute greater value to the significance of romantic relationships. Devoting greater empirical, educational, and community efforts to romantic development in the stages leading up to adulthood are considered necessary actions in promoting the well-being of young people.

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