Proliferative Properties and Cytokine Secretion of Lung Fibroblast Cell Lines of the Patients with Idiopathic Pulmonary Fibrosis

1998 ◽  
Vol 45 (1) ◽  
pp. 128
Author(s):  
Dong Soon Kim ◽  
Sang Hoon Paik ◽  
Kyung Yup Kong ◽  
Dong Kwan Kim ◽  
Seong Il Park ◽  
...  
Keyword(s):  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Cytokine Secretion ◽  
Fibroblast Cell Lines

scholarly journals Proteomic Analysis Reveals Key Proteins in Extracellular Vesicles Cargo Associated with Idiopathic Pulmonary Fibrosis In Vitro

Biomedicines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1058
Author(s):  
Juan Manuel Velázquez-Enríquez ◽  
Jovito Cesar Santos-Álvarez ◽  
Alma Aurora Ramírez-Hernández ◽  
Edilburga Reyes-Jiménez ◽  
Armando López-Martínez ◽  
...  
Keyword(s):  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Cell Lines ◽  
Extracellular Vesicles ◽  
Proteomic Analysis ◽  
Fibroblast Cell ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Collagen Chain ◽  
Fibroblast Cell Lines

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, irreversible, and highly fatal disease. It is characterized by the increased activation of both fibroblast and myofibroblast that results in excessive extracellular matrix (ECM) deposition. Extracellular vesicles (EVs) have been described as key mediators of intercellular communication in various pathologies. However, the role of EVs in the development of IPF remains poorly understood. This study aimed to characterize the differentially expressed proteins contained within EVs cargo derived from the fibroblast cell lines LL97A (IPF-1) and LL29 (IPF-2) isolated from lungs bearing IPF as compared to those derived from the fibroblast cell lines CCD8Lu (NL-1) and CCD19Lu (NL-2) isolated from healthy donors. Isolated EVs were subjected to label-free quantitative proteomic analysis by LC-MS/MS, and as a result, 331 proteins were identified. Differentially expressed proteins were obtained after the pairwise comparison, including all experimental groups. A total of 86 differentially expressed proteins were identified in either one or more comparison groups. Of note, proteins involved in fibrogenic processes, such as tenascin-c (TNC), insulin-like-growth-factor-binding protein 7 (IGFBP7), fibrillin-1 (FBN1), alpha-2 collagen chain (I) (COL1A2), alpha-1 collagen chain (I) (COL1A1), and lysyl oxidase homolog 1 (LOXL1), were identified in EVs cargo isolated from IPF cell lines. Additionally, KEGG pathway enrichment analysis revealed that differentially expressed proteins participate in focal adhesion, PI3K-Akt, and ECM–receptor interaction signaling pathways. In conclusion, our findings reveal that proteins contained within EVs cargo might play key roles during IPF pathogenesis.

Sacasg and sacalg: new GFP-labelled camel skin and lung fibroblast cell lines

2016 ◽  
Vol 23 (1) ◽  
pp. 73 ◽  
Author(s):  
Abdullah Alawad ◽  
Othman Alhazzaa ◽  
Mohammad Alkhrayef ◽  
Faisal Alagrafi ◽  
Ziyad Alhamdan ◽  
...  
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Fibroblast Cell Lines

scholarly journals The role of oxidative stress in 63 T-induced cytotoxicity against human lung cancer and normal lung fibroblast cell lines

2018 ◽  
Vol 37 (5) ◽  
pp. 849-864 ◽  
Author(s):  
Malgorzata Kucinska ◽  
Helena Mieszczak ◽  
Hanna Piotrowska-Kempisty ◽  
Mariusz Kaczmarek ◽  
Walter Granig ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lung Cancer ◽  
Cell Lines ◽  
Human Lung ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Human Lung Cancer ◽  
Normal Lung ◽  
Fibroblast Cell Lines

Codeletion of theJUN proto-oncogene and theCDKN2A tumor-suppressor gene inHRAS-transformed rat embryo fibroblast cell lines

1997 ◽  
Vol 20 (1) ◽  
pp. 82-89 ◽  
Author(s):  
Jian-Nian Zhou ◽  
Sofia Ljungdahl ◽  
Dan Röhme ◽  
Göran Levan ◽  
Maria C. Shoshan ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Tumor Suppressor Gene ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Suppressor Gene ◽  
Rat Embryo ◽  
Fibroblast Cell Lines

Measurement of DNA polymerase β in skin fibroblast cell lines from patients with ataxia telangiectasia

1985 ◽  
Vol 146 (3) ◽  
pp. 295-300 ◽  
Author(s):  
James B. Mitchell ◽  
Essam Karawya ◽  
Timothy J. Kinsella ◽  
Samuel H. Wilson
Keyword(s):  
Cell Lines ◽  
Dna Polymerase ◽  
Ataxia Telangiectasia ◽  
Skin Fibroblast ◽  
Fibroblast Cell ◽  
Dna Polymerase Β ◽  
Fibroblast Cell Lines
Sign in / Sign up

Export Citation Format

Share Document