scholarly journals Regenerative Therapies Using Cell Sheet-Based Tissue Engineering for Cardiac Disease

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Yuji Haraguchi ◽  
Tatsuya Shimizu ◽  
Masayuki Yamato ◽  
Teruo Okano
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Cardiac Disease ◽  
Heart Function ◽  
Cell Sheet ◽  
Three Dimensional ◽  
Tissue Cell ◽  
Myocardial Tissue ◽  
Therapeutic Effects ◽  
Cardiac Diseases

At present, cardiac diseases are a major cause of morbidity and mortality in the world. Recently, a cell-based regenerative medicine has appeared as one of the most potential and promising therapies for improving cardiac diseases. As a new generational cell-based regenerative therapy, tissue engineering is focused. Our laboratory has originally developed cell sheet-based scaffold-free tissue engineering. Three-dimensional myocardial tissue fabricated by stacking cardiomyocyte sheets, which are tightly interconnected to each other through gap junctions, beats simultaneously and macroscopically and shows the characteristic structures of native heart tissue. Cell sheet-based therapy cures the damaged heart function of animal models and is clinically applied. Cell sheet-based tissue engineering has a promising and enormous potential in myocardial tissue regenerative medicine and will cure many patients suffering from severe cardiac disease. This paper summarizes cell sheet-based tissue engineering and its satisfactory therapeutic effects on cardiac disease.

scholarly journals A Short Commentary Based on our Article Entitled “Capillary Networks for Bio-Artificial Three-Dimensional Tissues Fabricated Using Cell Sheet Based Tissue Engineering”

2021 ◽  
Vol 7 (2) ◽  
pp. 1-4
Author(s):  
Hidekazu Sekine ◽  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Cell Sheet ◽  
Three Dimensional ◽  
Small Scale ◽  
Waste Products ◽  
Cardiac Assist ◽  
Capillary Networks ◽  
Cardiac Assist Devices ◽  
Short Commentary

The development of organ-like tissues is the next major challenge facing regenerative medicine. Organ-like structures have been synthesized on a small scale, but overcoming current problems with cell sourcing and scalability will facilitate the creation of cardiac assist devices and organs for transplantation. A major obstacle to generating Three-Dimensional (3D) tissues is that diffusion is inadequate for the supply of oxygen/nutrients and removal of waste products, which limits the thickness of the structure to about 0.1 mm

scholarly journals Additive Manufacturing of Biopolymers for Tissue Engineering and Regenerative Medicine: An Overview, Potential Applications, Advancements, and Trends

2021 ◽  
Vol 2021 ◽  
pp. 1-20 ◽  
Author(s):  
Dhinakaran Veeman ◽  
M. Swapna Sai ◽  
P. Sureshkumar ◽  
T. Jagadeesha ◽  
L. Natrayan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Additive Manufacturing ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Porous Scaffolds ◽  
Wide Range ◽  
Potential Applications ◽  
Pharmaceutical Industries

As a technique of producing fabric engineering scaffolds, three-dimensional (3D) printing has tremendous possibilities. 3D printing applications are restricted to a wide range of biomaterials in the field of regenerative medicine and tissue engineering. Due to their biocompatibility, bioactiveness, and biodegradability, biopolymers such as collagen, alginate, silk fibroin, chitosan, alginate, cellulose, and starch are used in a variety of fields, including the food, biomedical, regeneration, agriculture, packaging, and pharmaceutical industries. The benefits of producing 3D-printed scaffolds are many, including the capacity to produce complicated geometries, porosity, and multicell coculture and to take growth factors into account. In particular, the additional production of biopolymers offers new options to produce 3D structures and materials with specialised patterns and properties. In the realm of tissue engineering and regenerative medicine (TERM), important progress has been accomplished; now, several state-of-the-art techniques are used to produce porous scaffolds for organ or tissue regeneration to be suited for tissue technology. Natural biopolymeric materials are often better suited for designing and manufacturing healing equipment than temporary implants and tissue regeneration materials owing to its appropriate properties and biocompatibility. The review focuses on the additive manufacturing of biopolymers with significant changes, advancements, trends, and developments in regenerative medicine and tissue engineering with potential applications.

scholarly journals Hydrogel Properties and Their Impact on Regenerative Medicine and Tissue Engineering

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5795
Author(s):  
Adam Chyzy ◽  
Marta E. Plonska-Brzezinska
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Physicochemical Properties ◽  
Three Dimensional ◽  
Modern Medicine ◽  
Wound Treatment ◽  
Chemical Modifications

Hydrogels (HGs), as three-dimensional structures, are widely used in modern medicine, including regenerative medicine. The use of HGs in wound treatment and tissue engineering is a rapidly developing sector of medicine. The unique properties of HGs allow researchers to easily modify them to maximize their potential. Herein, we describe the physicochemical properties of HGs, which determine their subsequent applications in regenerative medicine and tissue engineering. Examples of chemical modifications of HGs and their applications are described based on the latest scientific reports.

scholarly journals Physiological aspects of cardiac tissue engineering

2012 ◽  
Vol 303 (2) ◽  
pp. H133-H143 ◽  
Author(s):  
Thomas Eschenhagen ◽  
Alexandra Eder ◽  
Ingra Vollert ◽  
Arne Hansen
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cardiac Myocyte ◽  
Cardiac Tissue ◽  
Cell Sheet ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Basic Research ◽  
Cardiac Tissue Engineering ◽  
Predictive Toxicology

Cardiac tissue engineering aims at repairing the diseased heart and developing cardiac tissues for basic research and predictive toxicology applications. Since the first description of engineered heart tissue 15 years ago, major development steps were directed toward these three goals. Technical innovations led to improved three-dimensional cardiac tissue structure and near physiological contractile force development. Automation and standardization allow medium throughput screening. Larger constructs composed of many small engineered heart tissues or stacked cell sheet tissues were tested for cardiac repair and were associated with functional improvements in rats. Whether these approaches can be simply transferred to larger animals or the human patients remains to be tested. The availability of an unrestricted human cardiac myocyte cell source from human embryonic stem cells or human-induced pluripotent stem cells is a major breakthrough. This review summarizes current tissue engineering techniques with their strengths and limitations and possible future applications.

Cell Sheet-based Cardiac Tissue Engineering for Regenerative Medicine

2012 ◽  
Vol 18 (10) ◽  
pp. S145
Author(s):  
Katsuhisa Matsuura ◽  
Tatsuya Shimizu ◽  
Nobuhisa Hagiwara ◽  
Teruo Okano
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Cardiac Tissue ◽  
Cell Sheet ◽  
Cardiac Tissue Engineering

Engineered Biomimetic Nanofibers for Regenerative Medicine

2010 ◽  
Vol 76 ◽  
pp. 114-124
Author(s):  
Seeram Ramakrishna ◽  
Jayarama Reddy Venugopal ◽  
Susan Liao
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Cardiac Tissue ◽  
Three Dimensional ◽  
Electrospun Nanofibers ◽  
Cost Effective ◽  
Bioactive Molecules ◽  
Natural Polymers ◽  
Hematopoietic Stem

Attempts have been made to fabricate nanofibrous scaffolds to mimic the chemical composition and structural properties of extracellular matrix (ECM) for tissue/organ regeneration. Nanofibers with various patterns have been successfully produced from synthetic and natural polymers through a relatively simple technique of electrospinning. The resulting patterns can mimic some of the diverse tissue-specific orientation and three-dimensional (3D) fibrous structure. Studies on cell-nanofiber interactions have revealed the importance of nanotopography on cell adhesion, proliferation and differentiation. Our recent data showed that hematopoietic stem cells (HSCs) as well as mesenchymal stem cells (MSCs) can rapidly and effectively attached to the functionalized nanofibers. Mineralized 3D nanofibrous scaffold with bone marrow derived MSCs has been applied for bone tissue engineering. The use of injectable nanofibers for cardiac tissue engineering applications is attractive as they allow for the encapsulation of cardiomyocytes/MSCs as well as bioactive molecules for the repair of myocardial infarction. Duplicate 3D heart helix microstructure by the nanofibrous cardiac patch might provide functional support for infarcted myocardium. Furthermore, clinical applications of electrospun nanofibers for regenerative medicine are highly feasible due to the ease and flexibility of fabrication with the cost-effective method of making nanofibers.

Sign in / Sign up

Export Citation Format

Share Document