Aroma profile of pilot plant-scale produced fruit vinegar using a thermotolerant Acetobacter pasteurianus strain isolated from Moroccan cactus

In this study, new thermotolerant Acetobacter pasteurianus CV01 strain recently isolated from local product of Morocco has been investigated for its ability to perform efficient acetous fermentation at a large-scale. Firstly, the thermotolerance basis bioconversion of CV01 strain was compared to other mesophilic and thermotolerant acetic acid bacteria. Subsequently, CV01 strain was assessed for its ability to produce and tolerate high amount of acetic acid at optimal and thermal stress conditions in lab-scale bioreactor. It was found that the studied strain exhibited thermotolerant properties compared to reference strains and could withstand the increase in temperature during acetous fermentation in fermenter. Furthermore, gas chromatography-mass spectrometry (GC-MS) was used on the samples prepared with solid phase microextraction (SPME) to determine the volatile compounds of the pilot plant produced apple vinegar based on developed start-up and semi-continuous fermentation protocol. The operation strategy in the 500-L pilot plant scale acetator allowed achieving 7.3% (w/v) of final acetic acid concentration recording high yield and acetification rate. The aroma profile of experimentally produced vinegar was found different from that of the commercial reference one. According to the literature, the results obtained show that major volatile compounds found in pilot-plant produced apple vinegar are related to good aromatic note descriptors which could have a positive impact on the organoleptic quality of industrial vinegar. Consequently, it can be concluded that CV01 Acetobacter strain is well suited for large-scale production of high quality fruit vinegar.

Download Full-text

Analysis and Comparison of Mango Fermentation Carried out in Pilot Plate Scale Bioreactor and Acetator

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture ◽

10.15835/buasvmcn-agr:8792 ◽

2012 ◽

Vol 69 (2) ◽

Author(s):

Delia Mihaela TRUTA ◽

Maria TOFANÄ‚ ◽

Philippe THONART

Keyword(s):

Acetic Acid ◽

Volatile Compounds ◽

Pilot Plant ◽

Fermentation Process ◽

Mangifera Indica ◽

Acetic Acid Bacteria ◽

Acetic Acid Bacterium ◽

Fermentation Processes ◽

Bacteria Growth ◽

Pilot Plant Scale

Two mango vinegar fermentations have been carried out in a 20L pilot plant scale bioreactor and a 300L acetator. Both vinegar fermentations were started from fresh mango juice to witch was added the acetic acid bacteria Acetobacter senegalensis. This bacterium was isolated from mango fruits, Mangifera indica L., and was proved to have thermotolerance and acidotolerance properties. Based on this fact the acetic acid bacterium was used as a starter in the vinegar fermentation process. The aim of this research is compare the fermentation processes realized with the two equips, concerning the cells growth, the ethanol, sugars and organic acids evolution and also the volatile compounds. The volatile compounds have been identified by two gas chromatographic techniques, HS/GC-MS and HS/SPME-GC-MS, at different times during the two fermentation processes. The obtained results had shown a better mango vinegar fermentation process, regarding the bacteria growth, when the pilot plant scale bioreactor was used for the fermentation process rather than the acetator. Regarding the volatile compounds identified, a higher number was found by using the HS/SPME-GC-MS technique while the HS/GC-MS technique shown some variations of the volatile compounds identified during the fermentation processes.Â

Download Full-text

PILOT PLANT SCALE-UP STUDIES FOR PARENTERAL - A REVIEW

International Research Journal of Pharmacy ◽

10.7897/2230-8407.1208158 ◽

2021 ◽

Vol 12 (8) ◽

pp. 58-63

Author(s):

V. Manikandan

Keyword(s):

Pilot Plant ◽

Large Scale ◽

Scale Up ◽

Therapeutic Index ◽

The Body ◽

Plant Production ◽

Scale Production ◽

Large Scale Production ◽

Production Techniques ◽

Pilot Plant Scale

The dosage form of parenteral is sterile and gives a quick beginning of activity and gives an immediate action to accomplishing the medication impact inside the body. The route of parenteral administration is the most well-known and productive route for the conveyance of dynamic medication substances with poor bioavailability and medications with a tight therapeutic index. The principal objective of the technique was to endeavour to talk about the different procedures needed for the pilot plant production considers. The pilot plant is the term that is normally more modest than large-scale production plants yet it is the underlying scope of sizes. It is planned for learning, and making the definitions on a limited scale to accomplish the relationship with the enormous scope production, and they are normally more adaptable perhaps to the detriment of the economy. Most of the pilot plants are implicit in the maker's own research centres of the manufacturer utilizing stock lab hardware. These pilot plant studies are performed by using a technology transfer (TT) documentation report which is made by the research and development department for product development. Hence, this process would meet product quality, safety, and efficacy and further this production techniques will transfer to large-scale production for parenteral preparation.

Download Full-text

Isolation of Quercetin from Rubus fruticosus, Their Concentration through NF/RO Membranes, and Recovery through Carbon Nanocomposite. A Pilot Plant Study

BioMed Research International ◽

10.1155/2020/8216435 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Muhammad Zahoor ◽

Abdul Bari Shah ◽

Sumaira Naz ◽

Riaz Ullah ◽

Ahmed Bari ◽

...

Keyword(s):

Pilot Plant ◽

Large Scale ◽

Membrane System ◽

Scale Production ◽

Drain Pipe ◽

Rubus Fruticosus ◽

Large Scale Production ◽

Equilibrium Data ◽

Adsorption Equilibrium Data ◽

Carbon Nanocomposite

In this study, an attempt has been made to devise a method for a large-scale production of quercetin from a medicinal plant. The natural products are first isolated from plants and then synthesized commercially. During their synthesis, a number of impurities or side products are also formed, most of which are carcinogenic. Plant products have limited side effects. Therefore, they are considered safe to be used for systemic uses. In the Rubus fruticosus fruit, the ethyl acetate extract was loaded to 50 optimized silica gel columns. The effluents of columns were passed through the membrane system for concentration. A 100% recovery was achieved from the drain pipe in case of reverse osmosis membrane when the specified rely of the pilot plant was set on 25% rejection. About 95% recovery was achieved through the NF membrane while the 5% loss in permeate was recovered through magnetic carbon nanocomposite (characterized through a bar magnet, SEM, XRD, and EDX). The equilibrium time of adsorption was 83 min and followed by pseudo-first-order kinetics. The adsorption equilibrium data fitted well to the Langmuir isotherm model. Through the devised method, quercetin was successfully concentrated with high efficiencies; however, further studies are needed to validate the method.

Download Full-text

Large-scale production of peptides using the solid-phase continuous flow method. Preparative synthesis of the novel tachykinin antagonist MEN 10627

Journal of Peptide Science ◽

10.1002/(sici)1099-1387(199705)3:3<224::aid-psc104>3.0.co;2-z ◽

1997 ◽

Vol 3 (3) ◽

pp. 224-230 ◽

Cited By ~ 5

Author(s):

Valerio Caciagli ◽

Franco Cardinali ◽

Andre Hänsicke ◽

Gisbert Tuchalski ◽

Paolo Lombardi

Keyword(s):

Continuous Flow ◽

Large Scale ◽

Solid Phase ◽

Preparative Synthesis ◽

Scale Production ◽

The Novel ◽

Flow Method ◽

Large Scale Production ◽

Continuous Flow Method

Download Full-text

Scale-up of the production of highly reactive biogenic magnetite nanoparticles using Geobacter sulfurreducens

Journal of The Royal Society Interface ◽

10.1098/rsif.2015.0240 ◽

2015 ◽

Vol 12 (107) ◽

pp. 20150240 ◽

Cited By ~ 29

Author(s):

J. M. Byrne ◽

H. Muhamadali ◽

V. S. Coker ◽

J. Cooper ◽

J. R. Lloyd

Keyword(s):

Magnetic Properties ◽

Large Scale ◽

Scale Up ◽

Surface Reactivity ◽

Geobacter Sulfurreducens ◽

Scale Production ◽

Buffer Solution ◽

Pilot Plant Scale ◽

Functional Biomaterials ◽

Microbial Systems

Although there are numerous examples of large-scale commercial microbial synthesis routes for organic bioproducts, few studies have addressed the obvious potential for microbial systems to produce inorganic functional biomaterials at scale. Here we address this by focusing on the production of nanoscale biomagnetite particles by the Fe(III)-reducing bacterium Geobacter sulfurreducens , which was scaled up successfully from laboratory- to pilot plant-scale production, while maintaining the surface reactivity and magnetic properties which make this material well suited to commercial exploitation. At the largest scale tested, the bacterium was grown in a 50 l bioreactor, harvested and then inoculated into a buffer solution containing Fe(III)-oxyhydroxide and an electron donor and mediator, which promoted the formation of magnetite in under 24 h. This procedure was capable of producing up to 120 g of biomagnetite. The particle size distribution was maintained between 10 and 15 nm during scale-up of this second step from 10 ml to 10 l, with conserved magnetic properties and surface reactivity; the latter demonstrated by the reduction of Cr(VI). The process presented provides an environmentally benign route to magnetite production and serves as an alternative to harsher synthetic techniques, with the clear potential to be used to produce kilogram to tonne quantities.

Download Full-text

Bioreactor shoot cultures of Rhododendron tomentosum (Ledum palustre) for a large-scale production of bioactive volatile compounds

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-017-1261-0 ◽

2017 ◽

Vol 131 (1) ◽

pp. 51-64 ◽

Cited By ~ 12

Author(s):

Anna Jesionek ◽

Adam Kokotkiewicz ◽

Paulina Wlodarska ◽

Bozena Zabiegala ◽

Adam Bucinski ◽

...

Keyword(s):

Volatile Compounds ◽

Large Scale ◽

Scale Production ◽

Shoot Cultures ◽

Ledum Palustre ◽

Large Scale Production ◽

Rhododendron Tomentosum

Download Full-text

Dynamics of Volatile Compounds in Triticale Bread with Sourdough: From Flour to Bread

Foods ◽

10.3390/foods9121837 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1837

Author(s):

Ruta Galoburda ◽

Evita Straumite ◽

Martins Sabovics ◽

Zanda Kruma

Keyword(s):

Acetic Acid ◽

Volatile Compounds ◽

Solid Phase ◽

Nutritional Composition ◽

Gas Chromatography Mass Spectrometry ◽

Human Consumption ◽

Bread Making ◽

Two Stage ◽

Peak Areas ◽

Flour Blend

Triticale has been suggested for human consumption due to its valuable nutritional composition. The aim of this study was to evaluate volatile compound dynamics in the technological processes of triticale bread and triticale bread with sourdough prepared using Lactobacillus sanfranciscensis based cultures. Two types of sourdough ready-to-use sourdough and two-stage sourdough were used for bread making. Triticale bread without sourdough was used as a control. Volatile compounds from a headspace of flour blend, sourdough, as well as mixed dough, fermented dough, bread crumb and crust were extracted using solid-phase microextraction (SPME) in combination with gas chromatography/mass spectrometry. Alcohols, mainly 1-hexanol, were the main volatiles in the triticale flour blend, whereas in the headspace of sourdough samples ethyl-acetate, ethanol and acetic acid dominated. Two-stage sourdough after 30 min fermentation showed the highest sum of peak areas formed by 14 volatile compounds, resulting in substrates for further aroma development in bread. A total of 29 compounds were identified in the bread: in the crumb the dominant volatile compounds were alcohols, ketones, acids, but in the crust—alcohols, aldehydes, furans dominated. The use of two-stage sourdough provided a more diverse spectrum of volatile compounds. Such volatile compounds as ethanol, 3-methyl-1-butanol, 2-methyl-1-propanol, 2-hydroxy-2-butanone, 2-methylpropanoic acid, and acetic acid were identified in all the analysed samples in all stages of bread making.

Download Full-text

Identification of Aroma Compounds in Freeze-dried Strawberries and Raspberries by HS-SPME-GC-MS

Journal of Food Research ◽

10.5539/jfr.v9n4p30 ◽

2020 ◽

Vol 9 (4) ◽

pp. 30

Author(s):

Fadwa Al-Taher ◽

Boris Nemzer

Keyword(s):

Mass Spectrometry ◽

Volatile Compounds ◽

Solid Phase ◽

Aroma Compounds ◽

Gas Chromatography Mass Spectrometry ◽

Solid Phase Micro Extraction ◽

Aroma Profile ◽

Spme Fiber ◽

Freeze Dried ◽

Different Temperatures

The objective of this study was to determine a method for the identification of aroma volatile compounds in freeze-dried (FD) strawberries and raspberries for quality purposes. The aroma profile was examined using headspace solid-phase micro-extraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). FD strawberries and raspberries were extracted at four different times (10,15, 20 and 30 min) and three different temperatures (40 °C, 60 °C and 80 °C) using a SPME fiber coated with 50/30 µm divinylbenzene/carboxen on polydimethylsiloxane (DVB/CAR-PDMS) to determine optimum recoveries for aroma volatile compounds. The DVB/CAR-PDMS SPME fiber showed the best extraction of aroma volatile compounds from strawberry and raspberry at 60°C for 15 min. Twenty-nine volatile compounds were identified from the strawberry samples and 20 from the raspberry samples, including terpenes, aldehydes, esters, acids and alcohols. Select aroma compounds in FD strawberries and raspberries were quantitated using SPME and GC-MS. It is important to determine the desirable aroma active compounds in freeze-dried strawberries and raspberries for quality uses since they are becoming popular commercially.

Download Full-text

The Wexler Bend Pilot Plant

The Secret History of RDX ◽

10.5810/kentucky/9780813175287.003.0009 ◽

2018 ◽

Author(s):

Colin F. Baxter

Keyword(s):

United States ◽

World War Ii ◽

Mass Production ◽

Pilot Plant ◽

Large Scale ◽

The United States ◽

Scale Production ◽

World War ◽

Large Scale Production ◽

Scientific Achievements

One of the epic industrial and scientific achievements of the United States during World War II was accomplished at the Wexler Bend Pilot Plant, Kingsport, Tennessee, where fifty hand-picked (their finances were investigated, and all the operators were married with at least one child) Tennessee Eastman employees, working at a phenomenal pace, developed a process for large-scale production of the world’s most powerful explosive, RDX. The success at Wexler Bend opened the door to the next phase: the mass production of RDX.

Download Full-text