scholarly journals Metabolic activity of intestinal microflora in patients with bronchial asthma

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Vladimir Ivashkin ◽  
Oxana Zolnikova ◽  
Nino Potskherashvili ◽  
Alexander Trukhmanov ◽  
Natalia Kokina ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Bronchial Asthma ◽  
Metabolic Activity ◽  
Intestinal Microflora ◽  
Total Content ◽  
Short Chain Fatty Acids ◽  
Immunological Tolerance ◽  
Liquid Chromatographic Analysis ◽  
The Individual ◽  
Liquid Chromatographic

The gut microbiota plays the important role to support the immunological tolerance. To study a metabolic activity of the intestinal microflora the 44 patients suffering from bronchial asthma and 17 healthy volunteers were tested. The short-chain fatty acids (SCFA) spectrum was determined using the method of gas-liquid chromatographic analysis. We have detected a significant decrease the total content of the fatty acids in the feces (P<0.001), the absolute concentrations of the individual acids (acetate, propionate, butyrate; P<0.001) and the total content of isoacids (P<0.001) for all of the patients with bronchial asthma in the study, regardless of the phenotype. The anaerobic type of the SCFA spectrum was detected for the majority (83%) of the patients tested. The aerobic type of the SCFA metabolic profile was detected for another part (17%) of the patients tested. Being detected the parameters change could play the principal role as for the bronchial asthma course as to support the organism sensibilisation.

scholarly journals Intestinal Short-Chain Fatty Acids in Patients with Bronchial Asthma

2019 ◽  
Vol 29 (2) ◽  
pp. 53-59
Author(s):  
O. Yu. Zolnikova ◽  
N. D. Potskhverashvili ◽  
N. I. Kokina ◽  
A. S. Trukhmanov ◽  
V. T. Ivashkin
Keyword(s):  
Fatty Acids ◽  
Bronchial Asthma ◽  
Metabolic Profile ◽  
Clinical Phenotype ◽  
Fatty Acid Content ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic ◽  
Chain Fatty Acids

Aim. Tostudy the content and profile of short-chain fatty acids (SCFAs) in faeces of patients with bronchial asthma and healthy individuals, as well as to evaluate possible correlations between the SCFA spectrum and clinical phenotype of patients with bronchial asthma. Materials and methods. 44 patients with asthma and 17 healthy volunteers participated in the study. All participants underwent a generally accepted range of clinical and laboratory studies, as well as functional respiratory tests. The SCFA spectrum was determined using gas-liquid chromatographic analysis. The results of patients with asthma showed a significant decrease in the total fatty acid content in faeces (p <0.001); changes in the absolute concentrations of individual acids, such as acetate (p <0.001), propionate (p <0.001) and butyrate (p <0.001); as well as a change in the total isoacid content (p <0.001). In 83% of the cases, the anaerobic type of the SCFA spectrum was detected. The aerobic type of the SCFA metabolic profile was detected in 17% of the cases. The change in the metabolic profile did not depend on the phenotype of the disease.Conclusion. Changes in SCFAs indicate pronounced disorders in the microbiocenosis of the intestinal biotope. The values of the anaerobic index in the context of various changes in the acid metabolic profile indicate the disturbance of the microorganism’s habitat, contributing to the growth of anaerobic or aerobic microflora populations. 

scholarly journals Pathogenetic role of intestinal microflora in carbohydrate malabsorption syndrome in early-aged children with rotavirus infection

2021 ◽  
Vol 23 (5) ◽  
pp. 683-690
Author(s):  
N. V. Vorobiova ◽  
O. V. Usachova ◽  
A. H. Kaplaushenko
Keyword(s):  
Fatty Acids ◽  
Intestinal Microbiota ◽  
Metabolic Activity ◽  
Intestinal Microflora ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Malabsorption Syndrome ◽  
Rotavirus Infection ◽  
Carbohydrate Malabsorption ◽  
Chain Fatty Acids

The aim is to evaluate the effect of metabolic activity of intestinal microflora on laboratory manifestations of carbohydrate malabsorption syndrome in early-aged children with rotavirus infection by studying the main intestinal metabolites – short-chain fatty acids in faeces. Materials and methods. 60 breastfed children with rotavirus infection aged 1–24 months were examined. The total amount of carbohydrates in faecal samples was determined using Benedict’s test, and the detection of short-chain fatty acids in faeces was carried out using liquid chromatography in the dynamics of the disease – on the 3rd, 5th and 10th day. Results. It was established, that the amount of reducing sugars in faeces decreased with the increasing concentration of intestinal microflora metabolites during the entire period of rotavirus infection. In patients with ≤0.5 % level of carbohydrates in faeces, the total content of short-chain fatty acids was the highest and exceeded by 2.0, 1.8 and 1.7 times the indicators of children with Benedict’s test values >0.5 % on the 3rd, 5th and 10th days of the disease, respectively (p < 0.05). A decrease in the metabolic activity of the intestinal microbiota in the above category of children occurred mainly due to a deficiency in the production of acetic and propionic acids (P < 0.05). In the acute period of the disease, an imbalance in the intestinal microbiota infrastructure was noted towards a deficiency of obligate anaerobes that was expressed in a decrease in the anaerobic index (P < 0.05). The severity of these disorders influenced the severity of carbohydrate malabsorption: with an increase in the level of undigested sugars in faeces >0.5 %, the anaerobic index values were 3 and 5 times lower than at a carbohydrate level ≤0.5 % on the 3rd and 5th days of the disease, respectively (P < 0.05). Conclusions. It has been found that early-aged children with rotavirus infection have structural and functional disorders of the intestinal microflora, which influence the severity of carbohydrate malabsorption syndrome. The lower the saccharolytic activity of intestinal bacteria, the more pronounced the manifestations of this syndrome during the entire period of the disease.

scholarly journals The Comparison of Fatty Acid Composition and Lipid Quality Indices in Hard Cow, Sheep, and Goat Cheeses

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1667 ◽  
Author(s):  
Beata Paszczyk ◽  
Joanna Łuczyńska
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Total Content ◽  
Monounsaturated Fatty Acids ◽  
Short Chain Fatty Acids ◽  
Quality Indices ◽  
Chromatography Method ◽  
Lipid Quality

This study aimed to evaluate the fatty acid composition, including the contents, of conjugated linoleic acid cis9trans11 C18:2 (CLA) and trans C18:1 and C18:2 isomers in hard cow, sheep, and goat cheeses found on the Polish market and to compare lipid quality indices in these cheeses. The gas chromatography method was used to determine the fatty acid profile. The study demonstrated various contents of cis9trans 11 C18:2 (CLA), trans C18:1, and C18:2 isomers and the values of lipid quality indices in the cheeses. Sheep and goat cheeses were richer sources of short-chain fatty acids (SCFA) (14.73 ± 2.55% and 14.80 ± 2.80%, respectively) than the cow cheeses (9.38 ± 0.87%). The cow cheeses had a significantly higher (p < 0.05) content of monounsaturated fatty acids (MUFA), the lowest n-6/n-3 ratio, and the highest content of fatty acids, inducing a desirable dietary effect in humans (DFA) compared to the sheep and goat cheeses. Significantly higher (p < 0.05) contents of polyunsaturated fatty acids (PUFA) were found in sheep cheeses. Goat cheeses had the highest n-3 PUFA content and the lowest values of the thrombogenicity index (TI) (2.67 ± 0.44) compared to the sheep and cow cheeses (3.14 ± 0.29 and 3.13 ± 0.13, respectively). The cow, sheep, and goat cheeses were characterized by similar values of the hypocholesterolemic/hypercholesterolemic (H/H) ratio. Sheep cheeses had the highest levels of cis9trans11 C18:2 (CLA) and the highest total content of trans C18:1 and trans C18:2 isomers. The research showed that sheep, cow, and goat cheeses offered various health benefits. The differences in fatty acid composition and the different values of the lipid quality indices found in the cheeses may be due to differences in both the composition of milk used to produce them and the cheese-making technology. Studies conducted by many authors have indicated that the feeding system of the ruminants has a significant impact on the quality and chemical composition of milk, as well as its applicability for cheese production.

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