scholarly journals Green synthesis of Silver nanoparticles through Calotropis gigantea leaf extracts and evaluation of antibacterial activity against Vibrio alginolyticus

2012 ◽  
Vol 2 (1) ◽  
pp. 3 ◽  
Author(s):  
Vaseeharan Baskaralingam ◽  
Clara Gunapoorani Sargunar ◽  
Yong Chin Lin ◽  
Jiann Chu Chen
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Experimental Infection ◽  
Morphological Characteristics ◽  
Vibrio Alginolyticus ◽  
Effective Control ◽  
Nanoparticle Concentration ◽  
Leaf Extracts ◽  
Calotropis Gigantea ◽  
Green Synthesized Silver Nanoparticles

Green synthesized silver nanoparticles by <em>Calotropis gigantea</em> leaf extract were used to study the inhibitory activity against pathogenic <em>Vibrio alginolyticus</em>, isolated from wild <em>Artemia franciscana</em> cysts. Silver nanoparticle synthesis was observed using UV-visible spectroscopy and the morphological characteristics were analyzed by atomic force microscope (AFM). In the present study, increasing concentrations of silver nanoparticles synthesized on LB agar plates effectively reduced the number of colonies of <em>V. alginolyticus</em>. A decrease in colonies (CFUs) was observed at 5 mg/mL of silver nanoparticle concentration and the complete inhibition of V. alginolyticus was observed at 20 mg/mL of silver nanoparticle concentration on LB agar plates. In vivo controlling efficiency of silver nanoparticles was tested in an <em>A. franciscana</em> hatching system. Effective control of <em>V. alginolyticus</em> in brine shrimp <em>A. franciscana</em> hatching units was achieved by experimental infection and treatment with silver nanoparticles. Experimental infection studies showed that <em>V. alginolyticus</em> infected <em>Artemia</em> nauplii treated with silver nanoparticles (10 mg/mL) had greater survival (&gt;40%) than silver nanoparticles not treated with nauplii. Based on the findings of this study, it is recommended that low concentrations of green synthesized silver nanoparticles should be further investigated for other potential experimental models to control potential medical pathogens.

scholarly journals Влияние концентрации наночастиц серебра в функциональных покрытиях TiO-=SUB=-2-=/SUB=--Ag на характеристики фотопреобразователей GaInP/GaAs/Ge

2018 ◽  
Vol 52 (8) ◽  
pp. 860
Author(s):  
Л.С. Лунин ◽  
М.Л. Лунина ◽  
А.А. Кравцов ◽  
И.А. Сысоев ◽  
А.В. Блинов ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Solar Cells ◽  
Silver Nanoparticle ◽  
Optimum Concentration ◽  
Nanoparticle Concentration ◽  
Functional Coatings ◽  
Functional Characteristics

AbstractThe results of studying the effect of the silver nanoparticle concentration in TiO_2–Ag functional coatings on the characteristics of GaInP/GaAs/Ge photoconverters are discussed. The optimum concentration of silver nanoparticles in TiO_2 coatings, improving the functional characteristics of solar cells is determined.

scholarly journals Green synthesized silver nanoparticles using aqueous leaf extracts of Leucas aspera exhibits antimicrobial and catalytic dye degradation properties

2019 ◽  
Vol 1 (3) ◽  
Author(s):  
Muthusaravanan Sivaramakrishnan ◽  
Vivek Jagadeesan Sharavanan ◽  
Deenadayalan Karaiyagowder Govindarajan ◽  
Yogesan Meganathan ◽  
Bala Subramaniam Devaraj ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Dye Degradation ◽  
Leaf Extracts ◽  
Leucas Aspera ◽  
Degradation Properties ◽  
Green Synthesized Silver Nanoparticles

Applications of Green Synthesized Silver Nanoparticles Using Calotropis Gigantea

2021 ◽  
Vol 12 (1) ◽  
pp. 72-78
Author(s):  
Bhawna Nohwal, ◽  
Nitish Kumar ◽  
Reeti Chaudhary
Keyword(s):  
Silver Nanoparticles ◽  
Calotropis Gigantea ◽  
Green Synthesized Silver Nanoparticles

scholarly journals Antimicrobial Application and Assessment of Green Synthesized Silver Nanoparticles Using Aqueous Leaf Extract of Ficus Copiosa

2019 ◽  
Vol 6 (3) ◽  
pp. 06-15
Author(s):  
David Timi ◽  
Subramaniyam Gopalakrishnan ◽  
Macquin Maino
Keyword(s):  
Silver Nanoparticles ◽  
Sem Analysis ◽  
Leaf Extracts ◽  
Gram Positive ◽  
Gram Negative ◽  
Agar Disc ◽  
Diffusion Technique ◽  
Aqueous Leaf Extract ◽  
Micro Organisms ◽  
Green Synthesized Silver Nanoparticles

Plant mediated green-synthesized silver nanoparticles (AgNPs) and the bioactivity on pathogenic micro-organisms is discussed. In the present investigation, aqueous leaf extracts of a medicinal plant, Ficus copiosa was utilized to construct AgNPs. The synthesized AgNPs was characterized by Ultraviolet-visible (UV-vis), Fourier Transform-infrared (FT-IR), X-ray Diffraction (XRD) spectrometer and Scanning Electron Microscopy (SEM) analysis. The phytosynthesized AgNPs exhibited comparable antimicrobial activity with two reference antibiotics against five indicator micro-organisms including two gram-positive (G+) bacteria, two gram-negative (G-) bacteria and a Protozoa (Pz). Study of the minimum inhibition concentration shows the two gram-negative bacteria to be more susceptible to AgNPs than the two gram-positive bacteria. The organisms included in the study are namely, B. Subtilis (G+), S. aureus (G+), E. coli (G-), S. pneumonia (G-) and T. vaginalis (Pz). Agar Disc diffusion technique was employed to assess the efficacy of the silver nanoparticles on the micro-organisms.

Application of Gradient and Confocal Raman Spectroscopy to Analyze Silver Nanoparticle Diffusion in Medieval Glasses

2009 ◽  
Vol 8 ◽  
pp. 89-97 ◽  
Author(s):  
F. Rubio ◽  
Sofia Pérez-Villar ◽  
Miguel Angel Garrido ◽  
Juan Rubio ◽  
J.L. Oteo
Keyword(s):  
Raman Spectroscopy ◽  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Glass Surface ◽  
Microprobe Analysis ◽  
Glass Structure ◽  
Silver Ions ◽  
Nanoparticle Concentration ◽  
Confocal Raman Spectroscopy ◽  
Nanoparticle Diffusion

In this work it has been carried out the diffusion of silver ions in medieval glasses by a heat treatment process. Silver ions are transformed into both silver nanoparticles and nanoclusters after redox reactions with reducing glass ions. Changes in glass colour due to the formation of these silver nanoparticles have been analysed by means of visible spectroscopy. At the same time, changes in glass structure have been analysed by means of Raman scattering. By using confocal Raman spectroscopy the in deep glass structural changes occurring after silver ion diffusion and silver nanoparticle formation have been studied. These changes have been corroborated by means of gradient Raman spectroscopy where the silver ion and silver nanoparticle diffusion have been analysed on a fractured glass surface. In all cases have been observed that silver nanoparticles produce a depolymerisation of the glass structure and that such depolymerisation increases with the amount of silver nanoparticles. By using Microprobe Analysis it has been found that the higher silver nanoparticle concentration is on the glass surface and it decreases with the distance to the surface according to a diffusion process. By using nanoindentation measurements on original and gradient glass surfaces it has been found an increase of the Young modulus from 60 to 85 GPa, being the first value that corresponding to the glass surface with high silver nanoparticle concentration, and the second one for the glass without silver. This result is in accordance with Raman and Microprobe analysis.

scholarly journals Colorimetric Detection Based on Localized Surface Plasmon Resonance Optical Characteristics for Sensing of Mercury Using Green-Synthesized Silver Nanoparticles

2020 ◽  
Vol 2020 ◽  
pp. 1-14 ◽  
Author(s):  
Eman Alzahrani
Keyword(s):  
Silver Nanoparticles ◽  
Surface Plasmon ◽  
Silver Nanoparticle ◽  
Colorimetric Detection ◽  
Ultrasound Irradiation ◽  
Localized Surface Plasmon ◽  
Face Centered Cubic ◽  
Sem Images ◽  
Face Centered ◽  
Green Synthesized Silver Nanoparticles

Development of selective colorimetric detectors that can use green-fabricated silver nanoparticles’ (AgNPs) with localized surface plasmon resonances (LSPRs) to rapidly, simply, and selectively detect HgII ions was undertaken in this study. Onion extract was used for synthesising photo-induced green crystalline silver nanoparticles (NPs). The formation of nanoparticles is enhanced when ultrasound irradiation is present; bioligands could serve as stabilizing and reducing agents. Different methods of measurement, including UV-Vis, TEM,SEM/EDAX,FT−IR, and XRD, are effective for characterization of nanoparticles. The spherical nature of green-fabricated AgNPs is confirmed by TEM. High-density, spherical, and uniformly formed silver nanoparticle shapes were found in silver nanoparticle SEM images. The arrangement of AgNPs in the form of face-centered cubic structures was confirmed by XRD patterns. The formation of impurity-free AgNPs was confirmed using the EDAX analysis results. Hg2+ with excellent sensitivity was sensitively and selectively detected by employing green-synthesized silver nanoparticles. The reduction of Ag (1) to Ag (0) was confirmed by a slight increase in Hg (II) concentration and progressive reduction of green-synthesized AgNPs, whose absorbance changed abruptly. The reduction of LSPRs by the phosphate buffer medium enables AgNPs to sensitively and selectively detect Hg2+ ions by providing good environment. Besides, a selective, sensitive, simple, and rapid method that is proposed for detecting Hg (II) ions in samples of water is presented in the study. Harmful mercury ions in real samples of water (tap and ground water) can colorimetrically and selectively be detected using the AgNPs. The results showed an RSD of below 6% and over 92% of good recovery.

Green synthesized silver nanoparticles decorated on reduced graphene oxide for enhanced electrochemical sensing of nitrobenzene in waste water samples

RSC Advances ◽  
2015 ◽  
Vol 5 (39) ◽  
pp. 31139-31146 ◽  
Author(s):  
Chelladurai Karuppiah ◽  
K. Muthupandi ◽  
Shen-Ming Chen ◽  
M. Ajmal Ali ◽  
Selvakumar Palanisamy ◽  
...  
Keyword(s):  
Waste Water ◽  
Silver Nanoparticles ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Silver Nanoparticle ◽  
Electrochemical Sensing ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
Reduced Graphene ◽  
Green Synthesized Silver Nanoparticles

In the present work, an electrochemical sensor for nitrobenzene has been developed based on a green synthesized silver nanoparticle (AgNP) decorated reduced graphene oxide (RGO) modified glassy carbon electrode (GCE).

scholarly journals SYNTHESIS OF SILVER NANOPARTICLES FROM THE MEDICINAL PLANT BAUHINIA ACUMINATA AND BIOPHYTUM SENSITIVUM–A COMPARATIVE STUDY OF ITS BIOLOGICAL ACTIVITIES WITH PLANT EXTRACT

2016 ◽  
Vol 9 (1) ◽  
pp. 22 ◽  
Author(s):  
Elizabath Antony ◽  
Mythili Sathiavelu ◽  
Sathiavelu Arunachalam
Keyword(s):  
Silver Nanoparticles ◽  
Plant Extract ◽  
Silver Nanoparticle ◽  
Plant Extracts ◽  
Biological Activities ◽  
Alpha Tocopherol ◽  
Visible Spectroscopy ◽  
Leaf Extracts ◽  
Uv Visible ◽  
Dpph Scavenging

Objective: The aim of current study was to synthesise silver nanoparticles from the leaf extracts (aqueous and methanol) of two medicinal plants Bauhinia acuminata and Biophytum sensitivum and to compare its biological activities with that of plant extract.Methods: Silver nanoparticles were synthesised, and it was characterised using UV-Visible spectroscopy and scanning electron microscope (SEM). Fourier transform infrared spectroscopy (FTIR) and GC-MS analysis were done for silver nanoparticle extract. The biological activities such as DPPH scavenging assay, haemolytic assay and antimicrobial assay were done for both nanoparticle and plant extract.Results: The UV-Visible spectroscopy showed the formation of nanoparticles in a size range of 400-460 nm. GC-MS analysis showed the presence of biologically active compounds like DL-alpha-tocopherol and Alpha-tocopherol-beta-D-mannose. FTIR analysis of silver nanoparticles and leaf extracts showed the formation of aldehydes, alkenes, amines, alcohols, etc., which confirmed the presence of the compounds present in plant extracts. SEM image showed the formation of nanoparticles of size 2 micrometre. Phytochemical analysis of plant extracts showed the presence of carbohydrates, phenols, flavonoids, saponins, tannins and terpenoids. The methanol extract of Bauhinia acuminata showed high DPPH scavenging activity of 90% compared to that of the silver nanoparticle. The percentage hemolysis of all extracts was found to be 6%-39%. The antimicrobial activity of leaf extracts showed excellent activity towards Bacillus cereus and Listeria monocytogens.Conclusion: The results of present study showed that the silver nanoparticle synthesised from the plant extract has many bioactive compounds and it was found to have significant biological activities but comparatively lesser than plant extract. It concludes the both plant and nanoparticle extract can be used as a potential resource for therapeutic purpose.

scholarly journals Larvicidal Activity of Silver Nanoparticles Synthesized Using Extracts ofAmbrosia arborescens(Asteraceae) to ControlAedes aegyptiL. (Diptera: Culicidae)

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Bianca Morejón ◽  
Fernanda Pilaquinga ◽  
Flavia Domenech ◽  
Danny Ganchala ◽  
Alexis Debut ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Larvicidal Activity ◽  
Plant Extract ◽  
Mosquito Species ◽  
Cost Effective ◽  
Leaf Extracts ◽  
Suitable Alternative ◽  
Nontarget Effects ◽  
Primary Vector ◽  
Green Synthesized Silver Nanoparticles

The mosquito speciesAedes aegyptiis the primary vector of dengue, chikungunya, and Zika infections worldwide. Since effective vaccines or drugs are not available for the prevention and/or treatment of these pathologies, vector control has been adopted as the main approach to reduce their transmission. To controlAedespopulations, the most commonly used tool is the application of chemical insecticides and, despite their effectiveness, indiscriminate use of these chemicals has led to high operational costs, appearance of resistant populations, and adverse nontarget effects. Plant-derived insecticides may be an eco-friendly, cost-effective, and safe biocontrol alternative. The present study was carried out to evaluate the larvicidal activity of leaf extracts ofAmbrosia arborescensand green-synthesized silver nanoparticles (AgNPs) using aqueous extracts obtained from this plant against third instar larvae ofAe. aegypti. To test this, larvae were exposed for 24 h to the aqueous plant extract at 1500, 3000, 4500, and 6000 ppm and the plant-synthesized AgNPs at 0.2, 0.3, 0.4, and 0.5 ppm. In laboratory assays, AgNPs were more toxic (LC50 = 0.28 ppm; LC90 = 0.43 ppm) than the plant extract (LC50 = 1844.61 ppm; LC90 = 6043.95 ppm). These results suggest thatA. arborescensaqueous extract and green-synthesized silver nanoparticles produced from those extracts have the potential to be developed into suitable alternative tools useful for the control ofAe. aegyptipopulations.

scholarly journals Optimization of Selected Process Parameters Affecting Yield of Green Synthesized Silver Nanoparticles and Their Antibacterial Activity

2021 ◽  
pp. 25-36
Author(s):  
F. A. Ekaji ◽  
C. O. Akujobi ◽  
S. I. Umeh
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Process Parameters ◽  
Antibacterial Activities ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Box Behnken Design ◽  
E Coli ◽  
Optimized Conditions ◽  
Green Synthesized Silver Nanoparticles

Aims: To optimize effects of selected process parameters affecting yield of green synthesized silver nanoparticles and their antibacterial activity. Study Design: Study was designed with 3 factors Box Behnken Design (Minitab 17) and Response optimizer (Minitab 17) was used to determine optimum values of the factors. Place and Duration of Study: Department of Microbiology, Federal University of Technology, Owerri, Nigeria, from March to November, 2020. Methodology: After extraction by boiling, qualitative phytochemical analysis of leaves’ extracts of Ipeoma batatas, Commelina africana and Manihot esculenta was carried out. Following synthesis of silver nanoparticles as prescribed by Box Behnken design, yield of AgNPs was optimized with Response optimizer (Minitab 17). Then antibacterial activity of resulting AgNPs was tested against isolates of P. aeruginosa and E. coli. Results: Extracts contained alkaloids, tannins, proteins and amino acids, flavonoids and phenolic compounds, but no sterols and cardiac glycosides. Optimum pH, temperature and time obtained with Response Optimizer resulted in 62.6%, 55.8% and 54.9% increase in yield of AgNPs, with leaf extracts of C. africana, M. esculenta and I. batatas respectively, compared to un-optimized conditions. Absorbance for resulting AgNPs peaked between 380 to 400 nm. Zones of inhibition (mm) of P. aeruginosa with AgNPs synthesized using extracts of C. africana, I. batatas and M. esculenta were 10, 10 and 9 respectively, under un-optimized condition, and 12, 10 and 8 respectively, for optimized conditions. Against E. coli, they were 11, 11 and 12 for AgNPs synthesized with extracts of C. africana, I. batatas and M. esculenta respectively, under un-optimized condition, and 13, 9 and 11 respectively, for optimized conditions. Conclusion: Leaf extracts of C. africana, I. batatas and M. esculenta can be used in synthesizing AgNPs, with marked antibacterial activities. Box Behnken design is useful for optimization of effects of process parameters.

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