scholarly journals Comparing the minimum inhibitory and mutant prevention concentrations of selected antibiotics against animal isolates of Pasteurella multocida and Salmonella typhimurium

2022 ◽  
Vol 89 (1) ◽  
Author(s):  
Jeanette M. Wentzel ◽  
Louise J. Biggs ◽  
Moritz van Vuuren
Keyword(s):  
Salmonella Typhimurium ◽  
Pasteurella Multocida ◽  
Animal Isolates

scholarly journals STUDY ON PATHOGENICITY OF PASTEURELLA MULTOCIDA WHICH ISOLATED FROM FARM ANIMALS AND MAN

2021 ◽  
Vol 26 (1) ◽  
pp. 74-81
Author(s):  
S. S. A. Mobarak ◽  
A. K. Shubber ◽  
A. S. Raheem
Keyword(s):  
Pasteurella Multocida ◽  
Culture Media ◽  
Agar Medium ◽  
Farm Animals ◽  
Pathogenicity Test ◽  
Infected Farm ◽  
Human Samples ◽  
Selective Agar ◽  
Suppurative Pneumonia ◽  
Animal Isolates

This study was described for the nature of the pathpgenesis of bacteria Pasteurella multocida which was isolated from infected man made comparison between these bacteria and those from infected farm animals. The percentage of Pasteurella multcida diagnosed bacteria from animals and human was 29.4% and 16.9% respectively. Comparing to other culture media Pasteurella multocida selective agar medium was characterized by its selectivity and sensitivity and then was attempt for biotyping species and subspecies of isolated Pasteurella from animals and human samples were successfully achieved. Pathogenicity test was performed on mice, only nine human isolatetes and twenty-one animal isolates from Pasteurella multocida were virulent. Todistinguish between the pathogenesis of human and animal isolates, one isolated from human and animal were chosed, in addition to the standared strain. The mice had been experimentally infected by three different ways, I/P, I/T, I/Eye. The results were showed that Pasteurella multocida can produce lesions as fibrinous suppurative pneumonia in lungs, liver and spleen which were detected histopatho logically. However the animal isolates were more virulent than human or standared strain.

scholarly journals Haemophilus influenzae Clinical Isolates with Plasmid pB1000 Bearing blaROB-1: Fitness Cost and Interspecies Dissemination

2010 ◽  
Vol 54 (4) ◽  
pp. 1506-1511 ◽  
Author(s):  
Alvaro San Millan ◽  
Silvia Garcia-Cobos ◽  
Jose Antonio Escudero ◽  
Laura Hidalgo ◽  
Belen Gutierrez ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Pasteurella Multocida ◽  
Epidemiological Data ◽  
Clinical Isolates ◽  
Haemophilus Parasuis ◽  
Human Origin ◽  
Competitive Disadvantage ◽  
Derivative Plasmid ◽  
Lactamase Gene ◽  
Animal Isolates

ABSTRACT Plasmid pB1000 is a mobilizable replicon bearing the bla ROB-1 β-lactamase gene that we have recently described in Haemophilus parasuis and Pasteurella multocida animal isolates. Here we report the presence of pB1000 and a derivative plasmid, pB1000′, in four Haemophilus influenzae clinical isolates of human origin. Pulsed-field gel electrophoresis showed unrelated patterns in all strains, indicating that the existence of pB1000 in H. influenzae isolates is not the consequence of clonal dissemination. The replicon can be transferred both by transformation and by conjugation into H. influenzae, giving rise to recipients resistant to ampicillin and cefaclor (MICs, ≥64 μg/ml). Stability experiments showed that pB1000 is stable in H. influenzae without antimicrobial pressure for at least 60 generations. Competition experiments between isogenic H. influenzae strains with and without pB1000 revealed a competitive disadvantage of 9% per 10 generations for the transformant versus the recipient. The complete nucleotide sequences of nine pB1000 plasmids from human and animal isolates, as well as the epidemiological data, suggest that animal isolates belonging to the Pasteurellaceae act as an antimicrobial resistance reservoir for H. influenzae. Further, since P. multocida is the only member of this family that can colonize both humans and animals, we propose that P. multocida is the vehicle for the transport of pB1000 between animal- and human-adapted members of the Pasteurellaceae.

scholarly journals Isolation and evaluation of the probiotic potential of lactic acid strains from healthy equines for potential use in salmonellosis diarrhea

2020 ◽  
Author(s):  
Lulu Pei ◽  
Yang Hao ◽  
Ziyin Yan ◽  
Hui Zhang ◽  
Yanfang Lan ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Pasteurella Multocida ◽  
Tolerance Test ◽  
Future Application ◽  
Probiotic Properties ◽  
Pediococcus Acidilactici ◽  
Oral Toxicity ◽  
Differential Survival ◽  
Probiotic Potential

Abstract The objective of the present study was to evaluate the probiotic properties, security and antibacterial ability in vivo of isolated strains from healthy equine. In the present study, two Pediococcus acidilactici (P1 and P2) and two Lactobacillus equi (L1 and L2) were isolated. All isolates died when exposed to pH 2.0 for 3h but survived at pH 3.0 with differential survival. The viable bacteria count was sustained at high levels in a tolerance test with artificial gastrointestinal fluid. The isolates survived and grew at temperatures between 37 and 55 ℃ but died at 65 ℃. Four strains exhibited inhibitory activity against pathogens, including Salmonella typhimurium (CVCC542), Escherichia coli (C83902), Staphylococcus aureus (BNCC186335) and Pasteurella multocida (clinical isolate). These isolates exhibited differential antibiotic susceptibility. In safety trials, all isolates were γ-hemolytic, and the oral toxicity of strains P1 (gavaged with 1×109 CFU/day) and L1 (gavaged with 1×109 CFU/day) were analyzed in vivo. There were no effects on overall health status in mice. There were no significant differences in the incidence of bacteria translocation to blood, liver and spleen. Mice gavaged with Pediococcus acidilactici P1 (1×108 CFU/day) or Lactobacillus equi L1 (1×108 CFU/day) as prevention showed lower rates of diarrhea and mortality after challenged with Salmonella Typhimurium (4×106 CFU signal dose, 0.1 ml by intragastric gavage). The results indicate that the isolated strains could act as potential probiotics, providing a new way to reduce salmonella infection, which merit future application studies.

scholarly journals Study of Sensitivity and Resistivity of Salmonella typhimurium Isolated from Patient and Cattle to the Antibiotics and It s Relation with Plasmids

2009 ◽  
Vol 33 (2) ◽  
pp. 1-10
Author(s):  
Zainab Sami Habeeb
Keyword(s):  
Salmonella Typhimurium ◽  
Nalidixic Acid ◽  
Antimicrobial Agents ◽  
Resistant Isolate ◽  
First Line ◽  
Variable Sensitivity ◽  
Animal Isolates

The susceptibility of all Salmonella typhimurium isolates to the first line antimicrobial agents werestudied and the results obtained showed a variable sensitivity and resistivity since it shows e.gresistivity for human isolated were Ampicillin 100% ,Chloramphenicol 96% , Co-Trimoxazol 85%,Tetracycline 85%, Nalidixic acid 77% and Cefotaxime 33%, While in animal isolates they wereampicillin 57%, Co-Trimoxazol 14%, Chloramphenicol 26%, Tetracycline 42%, Nalidixic acid 85%and there was no resistant isolate to cefotaxime (0)%.Results obtained after the extration of the DNA plasmids revealed that acommon genetical bandshas been seen in addition to smaller ones that may differs in their appearance from astrain to anotheraccording to their antibiotic individual resistivity and sensitivity and this hypothesis may need a deeperand further studies to find prone relation .

scholarly journals Preparation of Local Diagnostic Kit for Salmonella typhimurium

2009 ◽  
Vol 33 (1) ◽  
pp. 44-50
Author(s):  
Zainab Sami Habeeb
Keyword(s):  
Immune Response ◽  
Salmonella Typhimurium ◽  
Widal Test ◽  
Hyperimmune Serum ◽  
Intravenous Injections ◽  
Animal Isolates

Some of somatic and flageller antgens from both the human and animal isolates were prepared,and were used to Immunize a group of rabbits by intravenous injections at an upgrading doses.Theresults of this immunization showed the gaining of an excellent hyperimmune serum. With the useof absorptions technique amonospecific antiserum was obtained for the specific injected antigenes, thestandardization of the antibodies showed a highly immune response reading. 1: 2560 , 1: 5120 to H:Iand O: 4.5 Antigens respectively for both antisera.This study showed the report for the 1st time the preparation for diagnostic kit for somatic and flagellarantigens to S. typhimurium comparing this method to Widal test with a good results and Response

Untersuchungen zur aeroben bakteriellen Besiedlung der Vaginalschleimhaut klinisch gesunder Kätzinnen

2004 ◽  
Vol 32 (02) ◽  
pp. 88-91
Author(s):  
Susanne Kloß ◽  
A. Wehrend ◽  
Astrid König ◽  
H. Bostedt
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Pasteurella Multocida ◽  
E Coli ◽  
Klinische Relevanz ◽  
Staphylococcus Intermedius

Zusammenfassung: Gegenstand und Ziel: Im Gegensatz zur Hündin liegen bei der Katze bisher wenige Studien über die genitale Keimflora geschlechtsgesunder Tiere vor. Ziel der Untersuchung war daher, physiologische Daten über die aerobe Vaginalflora bei dieser Spezies zu gewinnen. Material und Methoden: Für die vorliegende Studie standen 26 gesunde, anöstrische Katzen zur Verfügung, die zu einer Ovariohysterektomie vorgestellt wurden. Nach einer klinischen Untersuchung wurden von allen Probanden unter sterilen Bedingungen Vaginaltupfer entnommen. Ergebnisse: In allen Proben konnte ein Bakterienwachstum mit durchschnittlich zwei verschiedenen Bakterienspezies nachgewiesen werden. Die Gesamtkeimgehalte wurden bei 50% der Vaginaltupferproben als gering-, bei 15% als mittel- und bei 35% als hochgradig beurteilt. Vorherrschend waren Mischkulturen aus zwei bis vier verschiedenen Keimarten. Monokulturen wurden aus 38% der Tupferproben isoliert. Am häufigsten gelang der Nachweis von E. coli variatio haemolytica (E. coli var. haem.) (58%) und Staphylococcus epidermidis (42%). Als weitere Spezies wurden E. coli, α-, β-hämolysierende Streptokokken, anhämolysierende Streptokokken, aerobe Bazillen, Staphylococcus aureus, Staphylococcus intermedius, Pasteurella multocida sowie Klebsiellen isoliert. Auffällig ist die hohe Nachweisrate von E. coli var. haem. mit 35% in Mischkulturen und 23% in Reinkultur. Schlussfolgerungen: Die physiologische Mikroflora der felinen Vaginalschleimhaut differiert deutlich von der der anöstrischen Hündin. Besonders die Dominanz von E. coli var. haem. in 38% der Mischkulturen und 23% der Monokulturen bei der Katze ist hervorzuheben. Klinische Relevanz: Die vorliegenden Ergebnisse geben eine erste Grundlage für die Interpretation mikrobiologischer Befunde feliner Vaginaltupfer.

Bakterielle Meningitis mit Pasteurella multocida

2007 ◽  
Vol 34 (S 2) ◽  
Author(s):  
D Anhuf ◽  
T Klockgether ◽  
A Hartmann
Keyword(s):  
Pasteurella Multocida ◽  
Bakterielle Meningitis
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