Epidemiology of hospital acquired urinary tract infections in a medical college hospital in Goa

Background: A unique challenge for clinical microbiologists, clinicians, infection control professionals is to deal with extended-spectrum β-lactamase (ESBL) producing pathogens. The study was aims to isolate ESBL producing bacteria from urine samples of Urinary Tract Infection (UTI)-patients and to analyses their phenotypic and genotypic characteristics. Methods: A total of 90 urine samples from UTI patients were collected from Enam Medical College Hospital and Gonoshastha Medical College Hospital, Savar, Dhaka, Bangladesh; between May-2012 to August-2012. A total 75gram negative isolates were retrieved and screened for ESBL production by the Double Disk Diffusion Synergy Test (DDST). Isolates with ESBL phenotype were further characterised by antibiotic susceptibility testing, PCR and sequencing of β-lactamase genes. Results: Cultural and biochemical assay combined with 16S rRNA gene based phylogenetic identification confirmed that Escherichia spp. were predominant pathogens associate with UTI (41%), and the rest were distributed within the genus Enterobacter spp. 26%, Klebsiella spp. 21% , and Pseudomonas spp.10%. Total 31 isolates were phenotypically confirmed as ESBLs through DDST. The multidrug resistant (MDR) and ESBL producing bacteria showed high resistance to cefotaxime (96%), cefixime (90%) and imipenem (32%). PCR reaction was carried out targeting the genes blaTEM, blaCTX. Dominant ESBL class was CTX-M (65%) followed by TEM (52%). All ESBL isolates except 7 possesses multiple plasmids indicating possibility of both chromosomal and plasmid inheritance of ESBLs. Conclusions: This study shows a high prevalence of ESBL producing MDR in UTI patients among these two hospitals of Bangladesh indicating the necessity of alternative therapeutic intervention. Bangladesh Med Res Counc Bull 2019; 45: 23-33

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Antibiotic resistance pattern of bacteria causing urinary tract infection in a private medical college hospital, Dhaka

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v16i1.31131 ◽

2017 ◽

Vol 16 (1) ◽

pp. 42-47

Author(s):

Mahmuda Siddiqua ◽

Ahmed Nawsher Alam ◽

Sonia Akter ◽

Reena Saad Ferdousi

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Medical College Hospital ◽

Resistance Pattern ◽

College Hospital ◽

E Coli ◽

Medical College ◽

Tract Infections ◽

Resistance Rates

Background and objective: Urinary tract infection (UTI) is one of the frequently seen infections both in the world and in our country as well. Escherichia coli (E. coli) aremost frequently isolated in complicated or uncomplicated, nosocomial or community acquired urinary tract infections. To guide the empirical therapy, the resistance pattern of E. coli responsible was evaluated throughout the period in this study.Material and Method: Urine samples from outpatient / inpatient department of Ibn Sina Medical College Hospital between 1st January, 2015 and 31st December, 2015 were retrospectively analyzed. Presence of ?105 colony forming units/ml in urine culture was considered as significant for UTI. Isolated bacteria were identified by standard laboratory techniques and antibiotic susceptibility testing was performed by Kirby-Bauer disk diffusion method using Clinical Laboratory Standard Institute (CLSI) criteria.Result: A total of 271(13.4%) uropathogens were isolated. Overall E. coli accounted for 180 (66.42%) of all isolates. Resistance rates of E. coli to antimicrobial agents was demonstrated to be as follows: cefuroxime 82%, nalidaxic acid 74%, azithromycin 56%, cefotaxime52%, ceftazidime50%, cefixime 47%, cotrimoxazole 43%, ceftriaxone 41%, ciprofloxacin38%, amoxicillin- clavulanic acid 31%, cefepime30%, and low resistance which ranges from 9 to 1% included gentamycin 9%, meropenem 3%, imipenem2%, nitrofurantoin2% and amikacin 1%.Conclusion: As resistance rates show regional differences, it is necessary to regularly monitor regional resistance pattern to determine the appropriate empiric antibiotic treatment. The national antibiotic usage policies must be reorganized according to data obtained from these studies.Bangladesh Journal of Medical Science Vol.16(1) 2017 p.42-47

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Pattern of Bacteria Causing Urinary Tract Infections of inpatients and outpatients department at Anwer Khan Modern Medical College Hospital

Anwer Khan Modern Medical College Journal ◽

10.3329/akmmcj.v3i1.10107 ◽

1970 ◽

Vol 3 (1) ◽

pp. 13-15

Author(s):

T Shilpi ◽

SMA Huq ◽

M Khatun

Keyword(s):

Urinary Tract ◽

Specific Area ◽

Medical College Hospital ◽

Clinical Samples ◽

College Hospital ◽

Correct Treatment ◽

Klebsiella Species ◽

Medical College ◽

Important Health ◽

Tract Infections

The most common nosocomial infection is Urinary tract infection (UTI) among hospitalized patients as well as UTI is an important health problem in the community. The knowledge about the type of pathogens responsible for UTI and at a specific area may help the doctors to choose correct treatment regimen. This study was aimedat to investigate the pattern of isolated urinary pathogens. This study was done at Anwer Khan Modern Medical College Hospital, Dhaka during January- June, 2011. Out of 498 clinical samples of urine collected, 245 (49.19%) showed significant bacterial growth. The most common pathogens isolated were Escherichia coli (142, 58.0%), Streptococcus feacalis (38, 15.5%), Pseudomonus (20, 8.2%), Klebsiella species (20, 8.2%) and Staphylococcus epidermidis (14, 5.7%). The clinicians should use antibiotics according to pattern of causative agents that cause UTI in such setting. DOI: http://dx.doi.org/10.3329/akmmcj.v3i1.10107 AKMMCJ 2012; 3(1): 13-15

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Susceptibility to Imipenem/Relebactam of Pseudomonas Aeruginosa and Acinetobacter Baumannii Isolates From Chinese Intra-Abdominal, Respiratory and Urinary Tract Infections: SMART 2015 To 2018

10.21203/rs.3.rs-569240/v1 ◽

2021 ◽

Author(s):

Hui Zhang ◽

Peiyao Jia ◽

Ying Zhu ◽

Ge Zhang ◽

Jingjia Zhang ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Urinary Tract ◽

Acinetobacter Baumannii ◽

Urinary Tract Infections ◽

Surveillance Program ◽

College Hospital ◽

Medical College ◽

Carbapenem Resistant ◽

Tract Infections ◽

Abdominal Infections

Abstract Background In recent years, less options are available for treating carbapenem-resistant Acinetobacter baumannii and carbapenem-resistant Pseudomonas aeruginosa. The present study investigatesthe susceptibility rates to imipenem/relebactamfor the treatment of intra-abdominal infections (IAIs), respiratory tract infections (RTIs) and urinary tract infections (UTIs) caused by A. baumannii and P. aeruginosain China. Methods 1,886 P. aeruginosa and 1,889 A.baumanniiisolates were collected in 21 centers (7 regions) as part of the global SMART surveillance program between 2015 and 2018. Antimicrobial susceptibility testing was performed according tothe Clinical and Laboratory Standards Institute (CLSI) recommendations using the broth microdilution methodology in the Peking Union Medical College Hospital. Results Rates of imipenem-non-susceptibilities of P. aeruginosa and A. baumanniiisolates were 44.3% and 79.0%, whereas the multidrug-resistance (MDR) rateswere 44.3% and 81.9%, respectively.For P. aeruginosa, susceptibility rates to imipenem/relebactamwere 84.2% at aCLSI breakpoint of ≤ 2 mg/L compared to 55.7% for imipenem. The MIC90 of imipenem/relebactam(8 mg/L) was one fourth of that of imipenem (32 mg/L).The susceptibilities of imipenem-non-susceptible and MDRP.aeruginosastrains were similarly restored by imipenem/relebactam in non-ICU and ICU wards.The susceptiblity rate of A. baumannii isolates to imipenem was 21.0% and to imipenem/relebactam 22.2%. Conclusions Imipenem/relebactam provides a therapy option for infections caused by MDRor imipenem-non-susceptible P. aeruginosa infections in China.

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Prevalence of Urinary Tract Infection in Patient with Indwelling Catheter in Chittagong Medical College Hospital

Chattagram Maa-O-Shishu Hospital Medical College Journal ◽

10.3329/cmoshmcj.v17i1.39442 ◽

2018 ◽

Vol 17 (1) ◽

pp. 42-45

Author(s):

Raushan Akter ◽

Mahtab Uddin Hassan ◽

Rajat Sankar Roy Biswas

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Statistical Method ◽

Medical College Hospital ◽

Indwelling Catheter ◽

College Hospital ◽

Medical College ◽

Positive Data ◽

Hospital Acquired

Background: Hospital acquired urinary tract infection among the hospitalized catheterized patient due to different indications are a common morbility. The objective of study to see the prevalence hospital acquired urinary tract infection among the patients of indwelling catheter admitted in the department Medicine of Chittagong Medical College Hospital. Methods: It was a hospital based obsevational study conducted on purposively selected (Non-probability) hospitalized patients who got indwelling catheter had admitted into the Medicine ward, Chittagong Medical College Hospital from 1st January 2013 to 30th June 2013. Total 50 patients were selected. Urine was analyzed for growth of organism and sensitivity was also done in case where growth was positive. Data was analyzed by statistical method with the software SPSS-20. Results: Results showed that 15 (30%) developed bacteriuria or urinary tract infection with catheter. Development of bacteriuria was not affected by sex, age. People who introduce the catheter has some influence with the occurrence of UTI. E.coli, Klebsella, Proteas and Pseudomonas aeroginosa were the most common bacteria isolated. Imepenam was found the most sensitive antibiotic. Conclusion: Catheter play an important risk for occurrence of UTI. So it should be avoided if possible. Chatt Maa Shi Hosp Med Coll J; Vol.17 (1); Jan 2018; Page 42-45

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Isolation of Bacteria Causing Urinary Tract Infections and their Antibiotic Susceptibility Profile at Anwer Khan Modern Medical College Hospital

Anwer Khan Modern Medical College Journal ◽

10.3329/akmmcj.v4i2.16938 ◽

2013 ◽

Vol 4 (2) ◽

pp. 23-27 ◽

Cited By ~ 1

Author(s):

T Shilpi ◽

MN Ahmed ◽

SM Ariful Huq ◽

SK Baul ◽

M Khatun

Keyword(s):

Urinary Tract ◽

Antibiotic Susceptibility ◽

Medical College Hospital ◽

Clinical Samples ◽

Resistance Pattern ◽

College Hospital ◽

Correct Treatment ◽

Medical College ◽

Tract Infections ◽

Susceptibility And Resistance

Among hospitalized patients, the most common nosocomial infection is Urinary tract infection (UTI). The knowledge about the type of pathogens responsible for UTI and susceptibility and resistance pattern of the causative agents at a specific area may help the doctors to choose correct treatment regimen. This study was aimed to investigate the antibiotic susceptibility and resistance pattern of isolated urinary pathogens. This study was done at Anwer Khan Modern Medical College Hospital, Dhaka during January- June, 2011. Out of 498 clinical samples of urine collected, 245 (49.19%) showed significant bacterial growth. The most common pathogens isolated were Escherichia coli (142, 58.0%), Streptococcus feacalis (38, 15.5%), Pseudomonus (20, 8.2%), Klebsiella species (20, 8.2%) and Staphylococcus epidermidis (14, 5.7%). Members of the Enterobacteriaceae were 75%-100%sensitive to Amikacin and Nitrofurantoin while they were found variably sensitive to other commonly used antibiotics. Pseudomonas species were found 90% sensitive to Meropenem and 70% to Amikacin. Strep. feacalis were found 94.7% sensitive to Amoxicillin, 84.2% to Amoxiclave and 78.9% to Ciprofloxacin, 65.5% to Cephalexin, 50% to Ceftriaxone. The clinicians should use Meropenem and Amikacin selectively in cases of un-responsiveness to commonly used antibiotics. Anwer Khan Modern Medical College Journal Vol. 4, No. 2: July 2013, Pages 23-27 DOI: http://dx.doi.org/10.3329/akmmcj.v4i2.16938

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Pattern of Bacteria Causing Urinary Tract Infections and Their Antibiotic Susceptibility Profile at Chittagong Medical College Hospital

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v2i2.28842 ◽

2016 ◽

Vol 2 (2) ◽

pp. 17-21

Author(s):

MA Mazed ◽

Anwar Hussain ◽

Nasima Akter ◽

Tipu Sultan ◽

Arup Kanti Dewanje

Keyword(s):

Urinary Tract ◽

Medical College Hospital ◽

Clinical Samples ◽

Resistance Pattern ◽

College Hospital ◽

Correct Treatment ◽

Klebsiella Species ◽

Medical College ◽

Pseudomonas Species ◽

Tract Infections

Urinary tract infection (UTI) is the most common nosocomial infection among hospitalized patients. Area-specific monitoring studies aimed to gain knowledge about the type of pathogens responsible for UTIs and resistance pattern of the causative agents may help clinicians to choose correct treatment regimen. So, the present study was aimed to investigate the antibiotic resistance of urinary pathogens isolated at Chittagong Medical College Hospital (CMCH) during January to June, 2007. Out of 360 clinical samples of urine collected, 175 (48.61%) showed significant bacterial growth. The most common pathogens isolated were Escherichia coli (66, 37.71%), Klebsiella species (60, 34.29%), Proteus species (17, 9.71%) and Pseudomonas species (16, 9.14%). Members of the Enterobacteriaceae were 80%-100% sensitive to Imipenem while they were found variably sensitive to other commonly used antibiotics. Pseudomonas species were 100% sensitive to Imipenem, 75% to Amikacin, and 50% to Ceftazidime. Staphylococcus aureus was found 100% sensitive to Vancomycin, 80% to Gentamicin and Amikacin each, and 55% to Oxacillin. Enterococcus species were 80% sensitive to Imipenem, 60% to Ciprofloxacin, 50% to Vancomycin and Amikacin each. The clinicians should use Imipenem selectively in cases of un-responsiveness to commonly used antibiotics.Bangladesh J Med Microbiol 2008; 02 (02): 17-21

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