scholarly journals Characterization of extended spectrum β-Lactamase producing bactieria isolated from urinary tract infections

2019 ◽  
Vol 45 (1) ◽  
pp. 23-33
Author(s):  
Munawar Sultana ◽  
Anowar Khasru Parvez ◽  
Khandokar Fahmida Sultana ◽  
Sanjoy Kumer Mukharje ◽  
M Anwar Hossain
Keyword(s):  
Urinary Tract ◽  
Multidrug Resistant ◽  
Medical College Hospital ◽  
Urine Samples ◽  
Rrna Gene ◽  
College Hospital ◽  
Unique Challenge ◽  
Medical College ◽  
Extended Spectrum ◽  
Tract Infections

Background: A unique challenge for clinical microbiologists, clinicians, infection control professionals is to deal with extended-spectrum β-lactamase (ESBL) producing pathogens. The study was aims to isolate ESBL producing bacteria from urine samples of Urinary Tract Infection (UTI)-patients and to analyses their phenotypic and genotypic characteristics. Methods: A total of 90 urine samples from UTI patients were collected from Enam Medical College Hospital and Gonoshastha Medical College Hospital, Savar, Dhaka, Bangladesh; between May-2012 to August-2012. A total 75gram negative isolates were retrieved and screened for ESBL production by the Double Disk Diffusion Synergy Test (DDST). Isolates with ESBL phenotype were further characterised by antibiotic susceptibility testing, PCR and sequencing of β-lactamase genes. Results: Cultural and biochemical assay combined with 16S rRNA gene based phylogenetic identification confirmed that Escherichia spp. were predominant pathogens associate with UTI (41%), and the rest were distributed within the genus Enterobacter spp. 26%, Klebsiella spp. 21% , and Pseudomonas spp.10%. Total 31 isolates were phenotypically confirmed as ESBLs through DDST. The multidrug resistant (MDR) and ESBL producing bacteria showed high resistance to cefotaxime (96%), cefixime (90%) and imipenem (32%). PCR reaction was carried out targeting the genes blaTEM, blaCTX. Dominant ESBL class was CTX-M (65%) followed by TEM (52%). All ESBL isolates except 7 possesses multiple plasmids indicating possibility of both chromosomal and plasmid inheritance of ESBLs. Conclusions: This study shows a high prevalence of ESBL producing MDR in UTI patients among these two hospitals of Bangladesh indicating the necessity of alternative therapeutic intervention. Bangladesh Med Res Counc Bull 2019; 45: 23-33

scholarly journals Etiology of UTI and Frequency of ESBL Producing Bacteria Isolated from Patients of Dhaka Medical College Hospital with Their Antimicrobial Susceptibility Pattern

2017 ◽  
Vol 25 (1) ◽  
pp. 26-31
Author(s):  
Shahin Ara Begum ◽  
Shabeen Afreen ◽  
Farook Ahamed ◽  
Akhtaruzzaman Chowdhury ◽  
Mohammad Jobayer ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Bacterial Infections ◽  
Medical College Hospital ◽  
Urine Samples ◽  
Resistant Bacteria ◽  
Coagulase Negative Staphylococcus ◽  
Biochemical Tests ◽  
College Hospital ◽  
Medical College ◽  
Tract Infections

Background: Urinary tract infections (UTIs) are the most common of all bacterial infections and occur at any time in the life of an individual. ESBL producing bacteria particularly Escherichia.coli is one of the most common causes of UTIs both in community and healthcare associated settings. Emergence of multidrug resistance (MDR) is quite alarming and cause failure of empirical treatment of UTIs. As a result increase the morbidity and mortality rate in the developing countries like Bangladesh.Objective: The objective of this study was to find out the bacteria causing UTI from urine culture and detection of ESBL producing Esch.coli and K.pneumoniae with their anti- microbial susceptibility pattern.Materials and Methods: A total of 1750 urine samples were collected from patients with symptoms and suspected UTI. Clean catch mid-stream urine samples were collected from indoor and outdoor patients of Dhaka Medical College Hospital during January 2015 to July 2015.Urine specimens were cultured in 5% Blood agar and MacConkeys agar media. The isolated bacteria were identified by gram staining and biochemical tests. Antimicrobial susceptibility and detection of ESBL were done by disc diffusion method.Result: Out of 1750 urine samples, 403(23.03%) were positive by culture. Among the culture positive cases, 216 (53.59%) were female and 187 (46.41%) were male. The most common isolated bacteria were Esch.coli 295(73.20%) followed by Pseudomonas aeuroginosa 85(21.09%), K.pneumoniae10(2.48%), Proteus spp. 4(.99%), Acinetobacter spp. 5(1.2%), Coagulase negative Staphylococcus (CONS) 4(.99%). Among the isolated Esch.coli and K.pneumoniae, ESBL producing bacteria were 202 (68.47%) and 5 (50%) respectively. All the isolated bacteria showed low level susceptibility to all antibiotics that are used during the study period.Conclusion: Treatment of UTIs is difficult when caused by multidrug resistant bacteria. Analysis of culture and sensitivity data should be done periodically to identify ESBL producing bacteria for proper treatment of UTIs.J Dhaka Medical College, Vol. 25, No.1, April, 2016, Page 26-31

scholarly journals Antibiotic resistance pattern of bacteria causing urinary tract infection in a private medical college hospital, Dhaka

2017 ◽  
Vol 16 (1) ◽  
pp. 42-47
Author(s):  
Mahmuda Siddiqua ◽  
Ahmed Nawsher Alam ◽  
Sonia Akter ◽  
Reena Saad Ferdousi
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Medical College Hospital ◽  
Resistance Pattern ◽  
College Hospital ◽  
E Coli ◽  
Medical College ◽  
Tract Infections ◽  
Resistance Rates

Background and objective: Urinary tract infection (UTI) is one of the frequently seen infections both in the world and in our country as well. Escherichia coli (E. coli) aremost frequently isolated in complicated or uncomplicated, nosocomial or community acquired urinary tract infections. To guide the empirical therapy, the resistance pattern of E. coli responsible was evaluated throughout the period in this study.Material and Method: Urine samples from outpatient / inpatient department of Ibn Sina Medical College Hospital between 1st January, 2015 and 31st December, 2015 were retrospectively analyzed. Presence of ?105 colony forming units/ml in urine culture was considered as significant for UTI. Isolated bacteria were identified by standard laboratory techniques and antibiotic susceptibility testing was performed by Kirby-Bauer disk diffusion method using Clinical Laboratory Standard Institute (CLSI) criteria.Result: A total of 271(13.4%) uropathogens were isolated. Overall E. coli accounted for 180 (66.42%) of all isolates. Resistance rates of E. coli to antimicrobial agents was demonstrated to be as follows: cefuroxime 82%, nalidaxic acid 74%, azithromycin 56%, cefotaxime52%, ceftazidime50%, cefixime 47%, cotrimoxazole 43%, ceftriaxone 41%, ciprofloxacin38%, amoxicillin- clavulanic acid 31%, cefepime30%, and low resistance which ranges from 9 to 1% included gentamycin 9%, meropenem 3%, imipenem2%, nitrofurantoin2% and amikacin 1%.Conclusion: As resistance rates show regional differences, it is necessary to regularly monitor regional resistance pattern to determine the appropriate empiric antibiotic treatment. The national antibiotic usage policies must be reorganized according to data obtained from these studies.Bangladesh Journal of Medical Science Vol.16(1) 2017 p.42-47

scholarly journals Pattern of Bacteria Causing Urinary Tract Infections of inpatients and outpatients department at Anwer Khan Modern Medical College Hospital

1970 ◽  
Vol 3 (1) ◽  
pp. 13-15
Author(s):  
T Shilpi ◽  
SMA Huq ◽  
M Khatun
Keyword(s):  
Urinary Tract ◽  
Specific Area ◽  
Medical College Hospital ◽  
Clinical Samples ◽  
College Hospital ◽  
Correct Treatment ◽  
Klebsiella Species ◽  
Medical College ◽  
Important Health ◽  
Tract Infections

The most common nosocomial infection is Urinary tract infection (UTI) among hospitalized patients as well as UTI is an important health problem in the community. The knowledge about the type of pathogens responsible for UTI and at a specific area may help the doctors to choose correct treatment regimen. This study was aimedat to investigate the pattern of isolated urinary pathogens. This study was done at Anwer Khan Modern Medical College Hospital, Dhaka during January- June, 2011. Out of 498 clinical samples of urine collected, 245 (49.19%) showed significant bacterial growth. The most common pathogens isolated were Escherichia coli (142, 58.0%), Streptococcus feacalis (38, 15.5%), Pseudomonus (20, 8.2%), Klebsiella species (20, 8.2%) and Staphylococcus epidermidis (14, 5.7%). The clinicians should use antibiotics according to pattern of causative agents that cause UTI in such setting. DOI: http://dx.doi.org/10.3329/akmmcj.v3i1.10107 AKMMCJ 2012; 3(1): 13-15

scholarly journals Isolation of Bacteria Causing Urinary Tract Infections and their Antibiotic Susceptibility Profile at Anwer Khan Modern Medical College Hospital

2013 ◽  
Vol 4 (2) ◽  
pp. 23-27 ◽  
Author(s):  
T Shilpi ◽  
MN Ahmed ◽  
SM Ariful Huq ◽  
SK Baul ◽  
M Khatun
Keyword(s):  
Urinary Tract ◽  
Antibiotic Susceptibility ◽  
Medical College Hospital ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
College Hospital ◽  
Correct Treatment ◽  
Medical College ◽  
Tract Infections ◽  
Susceptibility And Resistance

Among hospitalized patients, the most common nosocomial infection is Urinary tract infection (UTI). The knowledge about the type of pathogens responsible for UTI and susceptibility and resistance pattern of the causative agents at a specific area may help the doctors to choose correct treatment regimen. This study was aimed to investigate the antibiotic susceptibility and resistance pattern of isolated urinary pathogens. This study was done at Anwer Khan Modern Medical College Hospital, Dhaka during January- June, 2011. Out of 498 clinical samples of urine collected, 245 (49.19%) showed significant bacterial growth. The most common pathogens isolated were Escherichia coli (142, 58.0%), Streptococcus feacalis (38, 15.5%), Pseudomonus (20, 8.2%), Klebsiella species (20, 8.2%) and Staphylococcus epidermidis (14, 5.7%). Members of the Enterobacteriaceae were 75%-100%sensitive to Amikacin and Nitrofurantoin while they were found variably sensitive to other commonly used antibiotics. Pseudomonas species were found 90% sensitive to Meropenem and 70% to Amikacin. Strep. feacalis were found 94.7% sensitive to Amoxicillin, 84.2% to Amoxiclave and 78.9% to Ciprofloxacin, 65.5% to Cephalexin, 50% to Ceftriaxone. The clinicians should use Meropenem and Amikacin selectively in cases of un-responsiveness to commonly used antibiotics. Anwer Khan Modern Medical College Journal Vol. 4, No. 2: July 2013, Pages 23-27 DOI: http://dx.doi.org/10.3329/akmmcj.v4i2.16938

scholarly journals Pattern of Bacteria Causing Urinary Tract Infections and Their Antibiotic Susceptibility Profile at Chittagong Medical College Hospital

2016 ◽  
Vol 2 (2) ◽  
pp. 17-21
Author(s):  
MA Mazed ◽  
Anwar Hussain ◽  
Nasima Akter ◽  
Tipu Sultan ◽  
Arup Kanti Dewanje
Keyword(s):  
Urinary Tract ◽  
Medical College Hospital ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
College Hospital ◽  
Correct Treatment ◽  
Klebsiella Species ◽  
Medical College ◽  
Pseudomonas Species ◽  
Tract Infections

Urinary tract infection (UTI) is the most common nosocomial infection among hospitalized patients. Area-specific monitoring studies aimed to gain knowledge about the type of pathogens responsible for UTIs and resistance pattern of the causative agents may help clinicians to choose correct treatment regimen. So, the present study was aimed to investigate the antibiotic resistance of urinary pathogens isolated at Chittagong Medical College Hospital (CMCH) during January to June, 2007. Out of 360 clinical samples of urine collected, 175 (48.61%) showed significant bacterial growth. The most common pathogens isolated were Escherichia coli (66, 37.71%), Klebsiella species (60, 34.29%), Proteus species (17, 9.71%) and Pseudomonas species (16, 9.14%). Members of the Enterobacteriaceae were 80%-100% sensitive to Imipenem while they were found variably sensitive to other commonly used antibiotics. Pseudomonas species were 100% sensitive to Imipenem, 75% to Amikacin, and 50% to Ceftazidime. Staphylococcus aureus was found 100% sensitive to Vancomycin, 80% to Gentamicin and Amikacin each, and 55% to Oxacillin. Enterococcus species were 80% sensitive to Imipenem, 60% to Ciprofloxacin, 50% to Vancomycin and Amikacin each. The clinicians should use Imipenem selectively in cases of un-responsiveness to commonly used antibiotics.Bangladesh J Med Microbiol 2008; 02 (02): 17-21

scholarly journals Epidemiology of hospital acquired urinary tract infections in a medical college hospital in Goa

2009 ◽  
Vol 25 (1) ◽  
pp. 76 ◽  
Author(s):  
UmeshS Kamat ◽  
Agnelo Fereirra ◽  
Dilip Amonkar ◽  
DilipD Motghare ◽  
ManojS Kulkarni
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Medical College Hospital ◽  
College Hospital ◽  
Medical College ◽  
Tract Infections ◽  
Hospital Acquired

scholarly journals Molecular Characterization of Multidrug Resistant Uropathogenic E. Coli Isolates from Jordanian Patients

2018 ◽  
Vol 12 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Yacoub R. Nairoukh ◽  
Azmi M. Mahafzah ◽  
Amal Irshaid ◽  
Asem A. Shehabi
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Multidrug Resistant ◽  
Urine Samples ◽  
Diffusion Method ◽  
Molecular Characteristics ◽  
Biochemical Tests ◽  
E Coli ◽  
Tract Infections ◽  
St131 Clone

Background: Emergence of multi-drug resistant uropathogenic E. coli strains is an increasing problem to empirical treatment of urinary tract infections in many countries. This study investigated the magnitude of this problem in Jordan. Methods: A total of 262 E. coli isolates were recovered from urine samples of Jordanian patients which were suspected to have urinary tract infections (UTIs). All isolates were primarily identified by routine biochemical tests and tested for antimicrobial susceptibility by disc diffusion method. Fifty representative Multidrug Resistance (MDR) E. coli isolates to 3 or more antibiotic classes were tested for the presence of resistance genes of blaCTX-M- 1, 9 and 15, carbapenemase (blaIMP, blaVIM, blaNDM-1, blaOXA-48), fluoroquinolones mutated genes (parC and gyrA) and clone of ST131 type using PCR methods. Results: A total of 150/262 (57.3%) of E. coli isolates were MDR. Urine samples of hospitalized patients showed significantly more MDR isolates than outpatients. Fifty representative MDR E. coli isolates indicated the following molecular characteristics: All were positive for mutated parC gene and gyrA and for ST131 clone, and 78% were positive for genes of CTX-M-15, 76% for CTX-M-I and for 8% CTX-M-9, respectively. Additionally, all 50 MDR E. coli isolates were negative for carbapenemase genes (blaIMP, blaVIM, blaNDM-1, blaOXA-48), except of one isolate was positive for blaKPC-2 . Conclusion: This study indicates alarming high rates recovery of MDR uropathogenic E. coli from Jordanian patients associated with high rates of positive ST131 clone, fluoroquinolone resistant and important types of blaCTX-M.

Microbiological Analysis of the Urine Isolates in Kathmandu Medical College Teaching Hospital, Kathmandu, Nepal

2012 ◽  
Vol 9 (4) ◽  
pp. 295-297 ◽  
Author(s):  
S Raza ◽  
S Pandey ◽  
C P Bhatt
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antimicrobial Agents ◽  
College Teaching ◽  
Urine Samples ◽  
Diffusion Method ◽  
Microbiological Analysis ◽  
Medical College ◽  
Antibiotic Susceptibility Test ◽  
Tract Infections

Background Urinary tract infections (UTIs) are the common cause of bacterial infection. Recently UTI become more complicated and difficult to treat because of appearance of pathogen with increasing resistance to antimicrobial agents. Objective To determine the etiology of the urinary tract infections and their susceptibility to antimicrobial agents. Methods This study was carried out in Kathmandu Medical College, at department of microbiology. Total 3,460 urine samples were tested microbiologically by standard procedure. Antibiotic susceptibility test was performed for all the isolates by Kirby Bauer disc diffusion method and result was interpreted according to National Committee for Clinical Laboratory Standards (NCCLS) guide line. Results Out of 3,460 urine samples 680 (19.7%) showed the significant bacteriuria. The most common pathogens isolated were Escherichia coli 75.7% followed by Klebsiella pneumoniae 10.7%, Acinetobacter spp 5.5%, Proteus spp 3.5% and Pseudomonas aeruginosa 1.2%. Most susceptible antibiotic was Amikacin, Ceftriaxone and Ciprofloxacin for most of the isolates. E. coli which was the main isolate was found to be most susceptible to Amikacin 96.1%, Nitrofurantoin 91.3% and Gentamicin 77.7% followed by Ceftriaxone 65.8% and Ciprofloxacin 64.1%. ConclusionRegular surveillance of the resistance rate among uro-pathogens is needed to ensure the appropriate therapy of UTI.DOI: http://dx.doi.org/10.3126/kumj.v9i4.6348 Kathmandu Univ Med J 2011;9(4):295-7 

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