Ultraviolet-B protective effect of flavonoids from Eugenia Caryophylata on human dermal fibroblast cells

Ultraviolet radiation (UV) is a major causative factor of DNA damage, inflammatory responses, reactive oxygen species (ROS) generation and a turnover of various cutaneous lesions resulting in skin photoaging. The purpose of this study is to investigate the protective effect of protocatechuic aldehyde (PA), which is a nature-derived compound, against UVA-induced photoaging by using human dermal fibroblast (HDF) cells. In this study, our results indicated that PA significantly reduced the levels of intracellular ROS, nitric oxide (NO), and prostaglandins-E2 (PGE2) in UVA-irradiated HDF cells. It also inhibited the levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression. Besides, PA significantly suppressed the expression of matrix metalloproteinases-1 (MMP-1) and pro-inflammatory cytokines and promoted collagen synthesis in the UVA-irradiated HDF cells. These events occurred through the regulation of activator protein 1 (AP-1), nuclear factor-κB (NF-κB), and p38 signaling pathways in UVA-irradiated HDF cells. Our findings suggest that PA enhances the protective effect of UVA-irradiated photoaging, which is associated with ROS scavenging, anti-wrinkle, and anti-inflammatory activities. Therefore, PA can be a potential candidate for the provision of a protective effect against UVA-stimulated photoaging in the pharmaceutical and cosmeceutical industries.

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Protective effect of crocin on ultraviolet B‑induced dermal fibroblast photoaging

Molecular Medicine Reports ◽

10.3892/mmr.2018.9150 ◽

2018 ◽

Cited By ~ 3

Author(s):

Mingwu Deng ◽

Dong Li ◽

Yichen Zhang ◽

Guangdong Zhou ◽

Wei Liu ◽

...

Keyword(s):

Protective Effect ◽

Dermal Fibroblast ◽

Ultraviolet B

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The Effect of Parietin Isolated From Rheum ribes L on In Vitro Wound Model Using Human Dermal Fibroblast Cells

The International Journal of Lower Extremity Wounds ◽

10.1177/1534734618819660 ◽

2019 ◽

Vol 18 (1) ◽

pp. 56-64 ◽

Cited By ~ 1

Author(s):

Gulsah Gundogdu ◽

Koksal Gundogdu ◽

Kemal Alp Nalci ◽

Alper Kursat Demirkaya ◽

Seymanur Yılmaz Tascı ◽

...

Keyword(s):

Cell Proliferation ◽

Cell Viability ◽

Dermal Fibroblast ◽

Dose Range ◽

Human Dermal Fibroblast ◽

Fibroblast Cells ◽

Wound Model ◽

Dpph Method ◽

Pipette Tip

Parietin is one of the well-known anthraquinone compounds that can be extracted from Rheum ribes L. In this study, we aimed to investigate the effects of parietin isolated from Rheum ribes L on an in vitro wound model using human dermal fibroblast cells and compare its effectiveness against zinc. The antioxidant effect of parietin was determined by using the 1,1-diphenyl-2-picrylhydrazine (DPPH) method. Human dermal fibroblast cells were cultured in proculture medium and were kept until 100% confluence was achieved. The wound model was created by using a pipette tip. After that, different concentrations of parietin and zinc (final concentrations in the well to be 5-250 µM and 25-200 µM, respectively) were added into the medium. The proliferation-inducing effect on cell viability was determined by using MTT assay. Images of cells were taken at 0, 12, and 24 hours. According to the DPPH method, parietin exhibited have antioxidant activity. According to the MTT results, parietin exhibited significant proliferation-inducing effect on cell viability in a dose range of 5 to 10 M, and zinc showed significant proliferation-inducing effect on cell viability at dose 50 µM ( P < .05). In addition, the image of cell proliferation was also shown at the same doses at 24 hours. In this study, we claim that parietin induces cell proliferation at low doses in cases of dermal fibroblast loss. In conclusion, parietin as an alternative to zinc in wound healing could be used by clinicians in the future with more extensive studies.

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