scholarly journals Protocatechuic Aldehyde Attenuates UVA-induced Photoaging in Human Dermal Fibroblast Cells by Suppressing MAPKs/AP-1 and NF-κB Signaling Pathways

2020 ◽  
Vol 21 (13) ◽  
pp. 4619
Author(s):  
Yuling Ding ◽  
Chanipa Jiratchayamaethasakul ◽  
Seung-Hong Lee
Keyword(s):  
Nitric Oxide ◽  
Protective Effect ◽  
Signaling Pathways ◽  
Inflammatory Responses ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Potential Candidate ◽  
Ros Scavenging ◽  
Protocatechuic Aldehyde ◽  
Hdf Cells

Ultraviolet radiation (UV) is a major causative factor of DNA damage, inflammatory responses, reactive oxygen species (ROS) generation and a turnover of various cutaneous lesions resulting in skin photoaging. The purpose of this study is to investigate the protective effect of protocatechuic aldehyde (PA), which is a nature-derived compound, against UVA-induced photoaging by using human dermal fibroblast (HDF) cells. In this study, our results indicated that PA significantly reduced the levels of intracellular ROS, nitric oxide (NO), and prostaglandins-E2 (PGE2) in UVA-irradiated HDF cells. It also inhibited the levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression. Besides, PA significantly suppressed the expression of matrix metalloproteinases-1 (MMP-1) and pro-inflammatory cytokines and promoted collagen synthesis in the UVA-irradiated HDF cells. These events occurred through the regulation of activator protein 1 (AP-1), nuclear factor-κB (NF-κB), and p38 signaling pathways in UVA-irradiated HDF cells. Our findings suggest that PA enhances the protective effect of UVA-irradiated photoaging, which is associated with ROS scavenging, anti-wrinkle, and anti-inflammatory activities. Therefore, PA can be a potential candidate for the provision of a protective effect against UVA-stimulated photoaging in the pharmaceutical and cosmeceutical industries.

scholarly journals Protective Effect of Diphlorethohydroxycarmalol Isolated from Ishige okamurae Against Particulate Matter-Induced Skin Damage by Regulation of NF-κB, AP-1, and MAPKs Signaling Pathways In Vitro in Human Dermal Fibroblasts

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1055 ◽  
Author(s):  
Lei Wang ◽  
Hyun Soo Kim ◽  
Jun-Geon Je ◽  
Jae Young Oh ◽  
Young-Sang Kim ◽  
...  
Keyword(s):  
Protective Effect ◽  
Signaling Pathways ◽  
Reactive Oxygen Species Generation ◽  
Brown Seaweed ◽  
Dermal Fibroblasts ◽  
Potential Candidate ◽  
Human Dermal Fibroblasts ◽  
Skin Damage ◽  
Ishige Okamurae ◽  
Hdf Cells

Particulate matters (PM), the main contributor to air pollution, have become a serious issue that threatens human’s health. Skin is the largest organ in humans, as well as the primary organ exposed to PM. Overexposure of PM induces skin damage. Diphlorethohydroxycarmalol (DPHC), an algal polyphenol with the potential of skin protection, has been isolated from the edible brown seaweed Ishige okamurae. The purpose of the present study is to investigate the protective effect of DPHC against PM (ERM-CZ100)-induced skin damage in human dermal fibroblasts (HDF) cells. The results indicated that DPHC significantly and dose-dependently reduced intracellular reactive oxygen species generation in HDF cells. In addition, DPHC significantly induced collagen synthesis and inhibited collagenase activity in ERM-CZ100-stimulated HDF cells. Further study demonstrated that DPHC remarkably reduced the expression of human matrix metalloproteinases through regulation of nuclear factor kappa B, activator protein 1, and mitogen-activated protein kinases signaling pathways in ERM-CZ100-stimulated HDF cells. This study suggested that DPHC is a potential candidate to protect skins against PM-induced damage, and it could be used as an ingredient in pharmaceutical and cosmeceutical industries.

scholarly journals Protective Effect of Sulfated Polysaccharides from Celluclast-Assisted Extract of Hizikia fusiforme Against Ultraviolet B-Induced Skin Damage by Regulating NF-κB, AP-1, and MAPKs Signaling Pathways In Vitro in Human Dermal Fibroblasts

Marine Drugs ◽  
2018 ◽  
Vol 16 (7) ◽  
pp. 239 ◽  
Author(s):  
Lei Wang ◽  
WonWoo Lee ◽  
Jae Oh ◽  
Yong Cui ◽  
BoMi Ryu ◽  
...  
Keyword(s):  
Protective Effect ◽  
Signaling Pathways ◽  
Dermal Fibroblasts ◽  
Sulfated Polysaccharides ◽  
Dependent Manner ◽  
Human Dermal Fibroblasts ◽  
Skin Damage ◽  
Hizikia Fusiforme ◽  
Hdf Cells

Our previous study evaluated the antioxidant activities of sulfated polysaccharides from Celluclast-assisted extract of Hizikia fusiforme (HFPS) in vitro in Vero cells and in vivo in zebrafish. The results showed that HFPS possesses strong antioxidant activity and suggested the potential photo-protective activities of HFPS. Hence, in the present study, we investigated the protective effects of HFPS against ultraviolet (UV) B-induced skin damage in vitro in human dermal fibroblasts (HDF cells). The results indicate that HFPS significantly reduced intracellular reactive oxygen species (ROS) level and improved the viability of UVB-irradiated HDF cells in a dose-dependent manner. Furthermore, HFPS significantly inhibited intracellular collagenase and elastase activities, remarkably protected collagen synthesis, and reduced matrix metalloproteinases (MMPs) expression by regulating nuclear factor kappa B (NF-κB), activator protein 1 (AP-1), and mitogen-activated protein kinases (MAPKs) signaling pathways in UVB-irradiated HDF cells. These results suggest that HFPS possesses strong UV protective effect, and can be a potential ingredient in the pharmaceutical and cosmetic industries.

scholarly journals Protective Effect of Processed Panax ginseng, Sun Ginseng on UVB-irradiated Human Skin Keratinocyte and Human Dermal Fibroblast

2012 ◽  
Vol 36 (1) ◽  
pp. 68-77 ◽  
Author(s):  
Hye-Jin Lee ◽  
Joo-Yeop Lee ◽  
Kyu-Choon Song ◽  
Jin-Hee Kim ◽  
Jeong-Hill Park ◽  
...  
Keyword(s):  
Protective Effect ◽  
Human Skin ◽  
Panax Ginseng ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast

scholarly journals In VitroWound Healing Potential and Identification of Bioactive Compounds fromMoringa oleiferaLam

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Abubakar Amali Muhammad ◽  
Nur Aimi Syarina Pauzi ◽  
Palanisamy Arulselvan ◽  
Faridah Abas ◽  
Sharida Fakurazi
Keyword(s):  
Wound Healing ◽  
Bioactive Compounds ◽  
Dermal Fibroblast ◽  
Bioactive Compound ◽  
Scratch Test ◽  
Human Dermal Fibroblast ◽  
Aqueous Fraction ◽  
Bioactive Fraction ◽  
Hdf Cells

Moringa oleiferaLam. (M. oleifera) from the monogeneric familyMoringaceaeis found in tropical and subtropical countries. The present study was aimed at exploring thein vitrowound healing potential ofM. oleiferaand identification of active compounds that may be responsible for its wound healing action. The study included cell viability, proliferation, and wound scratch test assays. Different solvent crude extracts were screened, and the most active crude extract was further subjected to differential bioguided fractionation. Fractions were also screened and most active aqueous fraction was finally obtained for further investigation. HPLC and LC-MS/MS analysis were used for identification and confirmation of bioactive compounds. The results of our study demonstrated that aqueous fraction ofM. oleiferasignificantly enhanced proliferation and viability as well as migration of human dermal fibroblast (HDF) cells compared to the untreated control and other fractions. The HPLC and LC-MS/MS studies revealed kaempferol and quercetin compounds in the crude methanolic extract and a major bioactive compound Vicenin-2 was identified in the bioactive aqueous fraction which was confirmed with standard Vicenin-2 using HPLC and UV spectroscopic methods. These findings suggest that bioactive fraction ofM. oleiferacontaining Vicenin-2 compound may enhance faster wound healingin vitro.

Antcamphin M Inhibits TLR4-Mediated Inflammatory Responses by Upregulating the Nrf2/HO-1 Pathway and Suppressing the NLRP3 Inflammasome Pathway in Macrophages

2019 ◽  
Vol 47 (07) ◽  
pp. 1611-1626 ◽  
Author(s):  
Wei-Hsiu Liu ◽  
Li-Shian Shi ◽  
Min-Chieh Chung ◽  
Tsu-Chung Chang ◽  
Shih-Yu Lee
Keyword(s):  
Signaling Pathways ◽  
Nlrp3 Inflammasome ◽  
Inflammatory Responses ◽  
Heme Oxygenase 1 ◽  
Prostaglandin E ◽  
Related Factor ◽  
Potential Candidate ◽  
Cytokines And Chemokines ◽  
Anti Inflammatory ◽  
Underlying Mechanisms

The medicinal mushroom Antrodia cinnamomea has been demonstrated to have anti-inflammatory properties. However, the bioactive compounds in A. cinnamomea need further investigation. The present study aimed to understand the mechanism of action of antcamphin M, an ergostanoid isolated from A. cinnamomea mycelium and to clarify its underlying mechanisms of action. RAW264.7 cells were pretreated with the indicated concentrations of antcamphin M, prior to stimulation with lipopolysaccharide (LPS). Cell viability, production of nitric oxide (NO), prostaglandin E2 (PGE[Formula: see text], cytokines, and chemokines, as well as the inflammation-related signaling pathways were investigated. The study revealed that antcamphin M significantly decreased the LPS-induced production of NO, PGE2, pro-inflammatory cytokines, and keratinocyte chemoattractant CXCL1 (KC), along with the levels of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins without significant cytotoxicity, indicating it had a better anti-inflammatory activity than that of gisenoside Rb1 and Rg1. Additionally, antcamphin M significantly inhibited the activation of MAPKs (p38, ERK, and JNK), NF[Formula: see text]B, and components of the NLRP3 inflammasome (NLRP3, ASC, and caspase-1) signaling pathways and also increased the levels of nuclear factor erythroid-2-related factor (Nrf2) and heme oxygenase-1 (HO-1). These findings suggest that antcamphin M possesses potent anti-inflammatory activities and could be a potential candidate for the development of anti-inflammatory drugs.

scholarly journals Biological Effect of Modern Fetal Ultrasound Techniques on Human Dermal Fibroblast Cells

2019 ◽  
Author(s):  
M Morshedi ◽  
M Bakhshandeh ◽  
A Piryaei ◽  
A Emami ◽  
M Zangeneh ◽  
...  
Keyword(s):  
Cell Viability ◽  
Doppler Sonography ◽  
Dermal Fibroblast ◽  
Color Doppler ◽  
Diagnostic Ultrasound ◽  
Human Dermal Fibroblast ◽  
Color Doppler Sonography ◽  
Fetal Ultrasound ◽  
Ki 67 ◽  
Hdf Cells

Background: Diagnostic ultrasound has been used to detect human disease especially fetus abnormalities in recent decades. Although the harmful effects of diagnostic ultrasound on human have not been established so far, several researchers showed it has had bioeffects in cell lines and in experimental animals. Three-dimensional (3D), four-dimensional (4D), and color Doppler sonography are new techniques which are widely used in diagnostic fetal ultrasonography.Objective: The study aims to evaluate some bioeffects of 3D, 4D, and color Doppler sonography in different exposure times according to the acoustic output which is set as ultrasound scanner’s default for fetal sonography in the second trimester on human dermal fibroblast (HDF) cells.Material and Methods: Exposure times selected consist of 10, 40, 70, and 100 seconds for 3D sonography, 10, 20, and 30 minutes for 4D sonography, and 10, 30, and 50 seconds for color Doppler. Cell viability, cell proliferation, and apoptosis induction on HDF cells were assessed using MTT assay, immunocytochemistry of Ki-67, and Terminal Transferase-mediated dUTP End-labeling (TUNEL) assay, respectively.Results: Exposure of cells to 3D, 4D, and color Doppler modes led to decreased cell viability and increased proliferation rate of HDF. None of the diagnostic ultrasound modes induced cell apoptosis.Conclusion: The results indicated that 3D, 4D, and color Doppler techniques may affect the cell viability and proliferation of HDF cells, however, have no effects on the induction of apoptosis probability. Further long-term studies with other molecular endpoints are required.

scholarly journals Bovine vitreous gel can reactivate replicative senescence of human dermal fibroblast

2018 ◽  
Vol 23 (1) ◽  
pp. 48
Author(s):  
Maria Vianny Sansan ◽  
Sunardi Radiono ◽  
Muhamad Eko Irawanto ◽  
Yohanes Widodo Wirohadidjojo
Keyword(s):  
Hyaluronic Acid ◽  
Replicative Senescence ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Dermal Fibroblasts ◽  
Influential Factor ◽  
Proliferation Index ◽  
Potential Candidate ◽  
Chronic Ulcers ◽  
Tgf Β1

The most influential factor in the poor healing of chronic ulcers is replicative senescence of fibroblasts that are unresponsive to TGF-β1 stimulation. Cellular replicative senescence can be induced by cultivating normal human dermal fibroblasts (HDFs) in a serum-starved medium. In addition, increasing microenviroment mechanical forces by hyaluronic acid can ameliorate the TGF-β1 signaling of these senescent cells. One of natural resources of hyaluronic acid is bovine vitreous gel. In order to evaluate the effect of bovine-vitreous gel on replicative senescence of fibroblasts, we used various levels of bovine vitreous gel diluted in Dulbecco’s modified Eagle’s medium to stimulate cellular activities of serum-starved HDFs. Those cellular activities were compared to the control media, standardized hyaluronic acid, and to normal HDFs. Our results show that replicative senescence of HDFs treated with 50% bovine vitreous gel exhibited a significantly higher proliferation index, migration rate, and collagen deposition than those cultured in control media, and they displayed an equal level of cellular activity with the HDFs exposed only to standardized hyaluronic acid. We concluded that bovine vitreous gel can be used to stimulate replicative senescence of HDFs and therefore a potential candidate material to stimulate healing of chronic ulcers.

scholarly journals Antioxidant Activity and Anti-Photoaging Effects on UVA-Irradiated Human Fibroblasts of Rosmarinic Acid Enriched Extract Prepared from Thunbergia laurifolia Leaves

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1648
Author(s):  
Thanawat Pattananandecha ◽  
Sutasinee Apichai ◽  
Jakaphun Julsrigival ◽  
Malyn Ungsurungsie ◽  
Suched Samuhasaneetoo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Rosmarinic Acid ◽  
Dermal Fibroblast ◽  
Human Fibroblasts ◽  
Potential Candidate ◽  
Normal Human Dermal Fibroblast ◽  
Matrix Metalloproteinase 1 ◽  
Thunbergia Laurifolia

The current study investigated the inhibiting effect on reactive oxygen species (ROS), reactive nitrogen species (RNS), and matrix metalloproteinase-1 (MMP-1) production in a cell-based study of standardized rosmarinic acid enriched extract (SRAEE) prepared from Thunbergia laurifolia leaves. HPLC chromatogram revealed that rosmarinic acid is a major component in prepared SRAEE, followed by caffeic acid. SRAEE exhibited antioxidant activity both in vitro and cell-based studies. SRAEE showed scavenging effects on nitric oxide and superoxide anion and inhibition effects on lipid peroxidation in vitro. SRAEE also inhibited ROS and MMP-1 production in normal human dermal fibroblast cells induced by H2O2 and UVA, respectively, without exerted cytotoxicity. Additionally, collagen degradation was protected by SRAEE induced by UVA. Nitric oxide and inducible nitric oxide synthase (iNOS) productions were also inhibited by SRAEE in RAW264.7 mouse macrophage cells induced by combined lipopolysaccharide (LPS)-interferon-γ (IFN-γ). The results indicated that SRAEE is a potential candidate as a natural pharmaceutical active ingredient for cosmeceutical product application.

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