Anti-inflammatory activity of ethanolic leaf extracts fromAdenanthera pavonina(l) in Rats

Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts. Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract. Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28- epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine-1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87 %) and H2O2 method (73.58%) at 200 µg mL-1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study. Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent antiinflammatory property.

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Effect of Cooking Temperature and Time Period on Phytochemical Content and in vitro Antioxidant and Anti-Inflammatory Activity of the Leaf Extracts of Typhonium trilobatum (A Less Focussed Edible Herbal Plant)

International Journal of Science and Research (IJSR) ◽

10.21275/art20164457 ◽

2017 ◽

Vol 6 (1) ◽

pp. 1540-1546

Keyword(s):

Inflammatory Activity ◽

Leaf Extracts ◽

Phytochemical Content ◽

Time Period ◽

Cooking Temperature ◽

Anti Inflammatory ◽

Herbal Plant

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Anti-inflammatory activity and phenolic composition of Dendropanax morbifera leaf extracts

Industrial Crops and Products ◽

10.1016/j.indcrop.2015.05.002 ◽

2015 ◽

Vol 74 ◽

pp. 263-270 ◽

Cited By ~ 30

Author(s):

Tae Kyung Hyun ◽

Yeong-Jong Ko ◽

Eun-Hye Kim ◽

Ill-Min Chung ◽

Ju-Sung Kim

Keyword(s):

Inflammatory Activity ◽

Phenolic Composition ◽

Leaf Extracts ◽

Dendropanax Morbifera ◽

Anti Inflammatory

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Comparative Study of Antimicrobial, Anti-Inflammatory, and Antioxidant Activities of Different Parts from Pterocarpus Santalinoides l’Her. Ex. DC (Fabaceae)

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/8938534 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Aimé Cézaire Ayéna ◽

Kokou Anani ◽

Kossi Dosseh ◽

Amegnona Agbonon ◽

Messanvi Gbeassor

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging ◽

Stem Bark ◽

Inflammatory Activity ◽

Bark Extract ◽

Leaf Extracts ◽

E Coli ◽

Broth Microdilution Method ◽

Anti Inflammatory ◽

Pterocarpus Santalinoides

Aims. Pterocarpus santalinoides is used in Beninese folk medicine for treatment of gastroenteritis. This study aims to compare the antimicrobial, antioxidant, and anti-inflammatory activity of the hydroalcoholic extracts of the leaves, trunk bark, and root. Materials and Methods. The antimicrobial activity was evaluated by the broth microdilution method on 06 bacterial strains including 03 wild-type strains (Escherichia. coli 0157H, Salmonella sp., and Staphylococcus aureus sp.) and 03 reference strains (E. coli ATCC 25922, S. aureus ATCC 29213, and Pseudomonas aeruginosa ATCC 27853), whereas the anti-inflammatory activity was performed by the carrageenan-induced paw edema method on rats. The DPPH-free radical scavenging was used to determine the antioxidant activity. Results. The MICs of the leaf extracts varied from 6.25 to 25 mg/mL for all strains. The MICs of the stem bark extracts were 6.5 to 25 μg/mL for five strains (E. coli 0157H, S. aureus ATCC 25922, Salmonella sp., E. coli ATCC 25922, and P. aeruginosa ATCC 27853) and 3.125 mg/mL for S. aureus. Concerning the root extracts, the MICs varied from 12.5 to 50 mg/mL. The best anti-inflammatory power was obtained with the stem bark extract with the percentages of inhibition of 36.09%, 38.98%, and 39.50%. The DPPH test showed that the hydroethanolic extract of the 03 parts of P. santalinoides has a moderate antiradical power compared to the control which was quercetin. Conclusion. In view of the different pharmacological activity recorded, the extract of the leaves should be recommended to treat patients suffering from gastroenteriditis.

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Antioxidant and Anti-Inflammatory Activities of Flavanones from Glycyrrhiza glabra L. (licorice) Leaf Phytocomplexes: Identification of Licoflavanone as a Modulator of NF-kB/MAPK Pathway

Antioxidants ◽

10.3390/antiox8060186 ◽

2019 ◽

Vol 8 (6) ◽

pp. 186 ◽

Cited By ~ 27

Author(s):

Luca Frattaruolo ◽

Gabriele Carullo ◽

Matteo Brindisi ◽

Sarah Mazzotta ◽

Luca Bellissimo ◽

...

Keyword(s):

Mapk Pathway ◽

Antioxidant Properties ◽

Health Spending ◽

Glycyrrhiza Glabra ◽

Inflammatory Activity ◽

Leaf Extracts ◽

Mitogen Activated Protein ◽

Natural Remedies ◽

Glycyrrhiza Glabra L ◽

Anti Inflammatory

Inflammation represents an adaptive response generated by injuries or harmful stimuli. Natural remedies represent an interesting alternative to traditional therapies, involving several biochemical pathways. Besides, the valorization of agrochemical wastes nowadays seems to be a feasible way to reduce the health spending and improve the accessibility at bioactive natural compounds. In this context, the chemical composition of three Glycyrrhiza glabra L. (licorice) leaf extracts, obtained through maceration or ultrasound-assisted method (fresh and dried leaves) was investigated. A guided fractionation obtained three main components: pinocembrin, glabranin and licoflavanone. All the extracts showed similar antioxidant properties, evaluated by 2,2′-diphenyl-1-picrylhydrazyl (DPPH) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Diammonium Salt (ABTS) assay, while, among the isolated compounds, licoflavanone exhibited the best antioxidant activity. The anti-inflammatory activity of the extracts and the purified compounds was investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophages. Extract C and licoflavanone showed a good anti-inflammatory activity without affecting cell viability, as they decreased nitrite levels even when used at 12.5 μg/mL (p < 0.005) and 50 μM concentration (p < 0.001), respectively. Interestingly, licoflavanone markedly decreased pro-inflammatory cytokines and cyclooxygenase 2/inducible nitric oxide synthase (COX-2/iNOS) expression levels (p < 0.001). A modulation of nuclear factor kappa B/mitogen-activated protein kinases (NF-kB/MAPK) pathway underlay such behavior, highlighting the potential of this natural compound as a new scaffold in anti-inflammatory drug research.

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Polyphenolic Profile, Antioxidant and Anti-Inflammatory Activity of Eastern Teaberry (Gaultheria procumbens L.) Leaf Extracts

Molecules ◽

10.3390/molecules191220498 ◽

2014 ◽

Vol 19 (12) ◽

pp. 20498-20520 ◽

Cited By ~ 18

Author(s):

Piotr Michel ◽

Anna Dobrowolska ◽

Agnieszka Kicel ◽

Aleksandra Owczarek ◽

Agnieszka Bazylko ◽

...

Keyword(s):

Inflammatory Activity ◽

Leaf Extracts ◽

Anti Inflammatory ◽

Polyphenolic Profile ◽

Gaultheria Procumbens

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The Anti-inflammatory Effect of Polyphenolics Olive Leaf Extracts Olea europaea L.

Phytothérapie ◽

10.3166/phyto-2021-0251 ◽

2021 ◽

Author(s):

Y. Aissaoui ◽

Y. Boukhari ◽

M. A. Mahi

Keyword(s):

Olea Europaea ◽

Acute Inflammation ◽

Inflammatory Activity ◽

Olive Leaves ◽

Leaf Extracts ◽

Polyphenolic Extract ◽

Significant Difference ◽

Anti Inflammatory ◽

Inflammatory Effect ◽

Olea Europaéa

This study was to evaluate the anti-inflammatory effect of the polyphenolic extract of olive leaves Olea europaea L. In vivo experiments were performed on the model of acute inflammation edema of the paw of male Wistar rats following inflammation induced by the injection of carrageenan. The anti-inflammatory effect is carried out by the injection of the polyphenolic extracts of olive leaves Olea europaea L. at different doses 50 mg/kg, 100 mg/kg and 150 mg/kg, the injection was carried out 30 min before induction of acute inflammation with carrageenan at 1%. The results obtained were compared with those of Diclofenac and those of the physiological control. Injection of carrageenan resulted in a significant increase in the claw leg volume of 46.24 ± 23.8%, 66.78 ± 29.69% and 90.31 ± 46.51%, respectively at 1 h, 2 h, and 3 h. The results showed that the inflammatory activity of the polyphenolic extracts of olive leaves was dose dependent. The 150 mg/kg Olea europaea L. extract of polyphenolic has an anti-inflammatory activity which results in an 88% decrease in edema. This decrease is significantly similar to that of Diclofenac® 97% confirmed that the polyphenolic extracts of Olea europaea L. leaves have anti-inflammatory activities comparable to that of Diclofenac® with a non-significant difference (p < 0.05), this anti-inflammatory effect remains lower than that of Diclofenac®.

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