Nigella sativa prevented liver and renal tissue damage in lipopolysaccharide-treated rats

The aim of this study is to investigate the protective effects of thymoquinone (TQ) and ebselen (Eb) on arsenic (As)-induced renal toxicity in female rats. Sodium arsenite was orally administrated at a dose of 20 mg/kg body weight daily for 28 days, either alone or 1 h before TQ (10 mg/kg) or Eb (5 mg/kg) administration. Renal tissue As concentration and oxidative stress markers, including lipid peroxidation (LPO), nitrite/nitrate, and glutathione (GSH) levels, were determined. In addition to the oxidative stress response, antioxidant enzyme activities including that of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase were measured. Exposure to As elicited a significant increase in As concentration and significant modifications to the redox state of the kidney, as was evidenced by a significant elevation in LPO and nitrite/nitrate concentration, with a concomitant reduction in GSH content and antioxidant enzyme activity. The oxidant/antioxidant imbalance observed in As toxicity was associated with a significant elevation in renal tumor necrosis factor α, interleukin 6, B-cell lymphoma 2 (Bcl-2)-associated X protein, and caspase 3 levels, in addition to a significant decrease in Bcl-2 levels. Post-administration of TQ and Eb markedly prevented As-induced oxidative stress, inflammation, apoptosis, and As accumulation in the renal tissue and reduced histological renal damage. These findings demonstrate that TQ, the main bioactive phytochemical constituent of Nigella sativa seed oil, and Eb, an organoselenium compound, could significantly inhibit As-induced oxidative damage, apoptosis, and inflammation, and significantly attenuate the accumulation of As in renal tissues by facilitating As biomethylation and excretion.

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Amelioration of Inflammation Induced Oxidative Stress and Tissue Damage by Aqueous Methanolic Extract of Nigella sativa Linn. in Arthritic Rats

Journal of Complementary and Integrative Medicine ◽

10.2202/1553-3840.1454 ◽

2010 ◽

Vol 7 (1) ◽

Cited By ~ 3

Author(s):

Mir Sajad ◽

Mohd Asif ◽

Sadiq Umar ◽

Jamil Zargan ◽

Mohd Rizwan ◽

...

Keyword(s):

Oxidative Stress ◽

Tissue Damage ◽

Methanolic Extract ◽

Nigella Sativa

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Thymoquinone Upregulates Catalase Gene Expression and Preserves the Structure of the Renal Cortex of Propylthiouracil-Induced Hypothyroid Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/3295831 ◽

2020 ◽

Vol 2020 ◽

pp. 1-15

Author(s):

Nasra Ayuob ◽

Maha Jameal Balgoon ◽

Ahmed A. El-Mansy ◽

Wafaa A. Mubarak ◽

Alaa El-Din L. Firgany

Keyword(s):

Gene Expression ◽

Body Weight ◽

Protective Effect ◽

Renal Cortex ◽

Nigella Sativa ◽

Serum Levels ◽

Renal Tissue ◽

Renal Diseases ◽

Catalase Gene ◽

Male Wistar Rats

Background. The association between hypothyroidism and renal diseases has been described in many studies. Nigella Sativa was among the recently reported natural product that has the potential to prevent renal tissue damage and fibrosis. The aim of this study was to evaluate the possible protective effect of thymoquinone on the structure of the renal cortex of hypothyroid rats and explore the mechanism behind it. Methods. An experimental model of hypothyroidism was induced in adult male Wistar rats by administration of propylthiouracil (6 mg/kg/body weight). One hypothyroid group was treated with thymoquinone at the dose of 50 mg/kg/body weight and compared to the untreated group. Thyroid function and oxidant/antioxidant status were assessed in the serum. Catalase gene expression was assessed using the real-time polymerase chain reaction. The kidney was assessed both histologically and immunohistochemically. Results. Administration of propylthiouracil resulted in a significant decrease in the serum levels of nitric oxide, reduced glutathione, and superoxide dismutase activity while the level of malondialdehyde significantly (p<0.001) increased. Administration of thymoquinone alleviated this effect on the thyroid hormones and significantly increased the serum levels of antioxidants. Thymoquinone significantly (p<0.001) upregulated catalase transcription by about 24-fold and could block the hypothyroidism-induced glomerular and tubular injury. Conclusion. Thymoquinone may have a potential protective effect against hypothyroidism-induced renal injury acting through the attenuation of the oxidative stress and upregulation of renal catalase gene expression.

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Protective effects of pentoxifylline on cigarette smoking-induced renal tissue damage in rats

Toxicology and Industrial Health ◽

10.1177/0748233710387006 ◽

2010 ◽

Vol 27 (4) ◽

pp. 335-340 ◽

Cited By ~ 1

Author(s):

Cuneyd Ozkurkcugil ◽

Melda Yardimoglu Yilmaz ◽

Levend Ozkan ◽

Sibel Kokturk ◽

Tonguc Isken

Keyword(s):

Cigarette Smoking ◽

Tissue Damage ◽

Renal Tissue ◽

Protective Effects

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Is enalapril adequate for the prevention of renal tissue damage caused by unilateral ureteral obstruction and/or hyperoxaluria?

Urological Research ◽

10.1007/s00240-003-0320-7 ◽

2003 ◽

Vol 31 (3) ◽

pp. 212-217 ◽

Cited By ~ 4

Author(s):

T. Turan ◽

J. G. van Harten ◽

R. de Water ◽

O. L. Tuncay ◽

D. J. Kok

Keyword(s):

Tissue Damage ◽

Ureteral Obstruction ◽

Renal Tissue ◽

Unilateral Ureteral Obstruction

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Mesenteric Lymph Drainage Alleviates Acute Kidney Injury Induced by Hemorrhagic Shock without Resuscitation

The Scientific World JOURNAL ◽

10.1155/2014/720836 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Zi-Gang Zhao ◽

Hong-Xia Zhu ◽

Li-Min Zhang ◽

Yu-Ping Zhang ◽

Chun-Yu Niu

Keyword(s):

Acute Kidney Injury ◽

Hemorrhagic Shock ◽

Tissue Damage ◽

Kidney Injury ◽

Renal Tissue ◽

Intercellular Adhesion Molecule ◽

Intercellular Adhesion Molecule 1 ◽

Lymph Drainage ◽

Mesenteric Lymph ◽

2,3 Dpg

This study aimed to investigate the effect of mesenteric lymph drainage on the acute kidney injury induced by hemorrhagic shock without resuscitation. Eighteen male Wistar rats were randomly divided into sham, shock, and drainage groups. The hemorrhagic shock model (40 mmHg, 3 h) was established in shock and drainage groups; mesenteric lymph drainage was performed from 1 h to 3 h of hypotension in the drainage group. The results showed that renal tissue damage occurred; the levels of urea, creatinine, and trypsin in the plasma as well as intercellular adhesion molecule-1 (ICAM-1), receptor of advanced glycation end-products (RAGE), tumor necrosis factor-α(TNF-α), malondialdehyde (MDA), lactic acid (LA), and 2,3-DPG in the renal tissue were increased in the shock group after 3 h of hypotension. Mesenteric lymph drainage lessened the following: renal tissue damage; urea and trypsin concentrations in the plasma; ICAM-1, RAGE, TNF-α, MDA, and LA levels in the renal tissue. By contrast, mesenteric lymph drainage increased the 2,3-DPG level in the renal tissue. These findings indicated that mesenteric lymph drainage could relieve kidney injury caused by sustained hypotension, and its mechanisms involve the decrease in trypsin activity, suppression of inflammation, alleviation of free radical injury, and improvement of energy metabolism.

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