Evaluation of polymerase chain reaction (PCR) as a diagnostic technique for acanthamoebic keratitis

2016 ◽  
Vol 9 (2) ◽  
pp. 87
Author(s):  
FatenA Mohammed ◽  
LailaA Aboul-Magd ◽  
BeessaE Abaza ◽  
WaleedM Nada ◽  
AfafA Taha ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Diagnostic Technique ◽  
Chain Reaction ◽  
Acanthamoebic Keratitis ◽  
Polymerase Chain

scholarly journals Nested Polymerase Chain Reaction (Nested-PCR) As A Diagnostic Technique for Intestinal Helminth Infection

2020 ◽  
Vol 5 (4) ◽  
pp. 183
Author(s):  
Reqgi First Trasia
Keyword(s):  
Polymerase Chain Reaction ◽  
Helminth Infection ◽  
Diagnostic Technique ◽  
Physical Growth ◽  
Intestinal Helminth ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Helminth Infections ◽  
Appropriate Treatment ◽  
Polymerase Chain

<p><em>Abstract</em> - <strong>Worm infections are still a health problem in the world, involving Indonesia. This disease generally rarely causes death, it has a detrimental impact on society such as malnutrition, anemia, productivity declining, and inhibits physical growth and intelligence in children. Efforts to support the intestinal helminth infection elimination program must be supported by proper diagnosis so that it can be continued with appropriate treatment and as a guideline for determining further policy. Microscopic examination is currently still the most commonly used method but has a limitation, especially less sensitive if in conditions of low infection. Molecular diagnoses suc [1]h as PCR can provide an alternative in providing a more sensitive and specific diagnosis of intestinal helminth infection.</strong></p><p><strong>Keywords -<em> Diagnosis, Intestinal helminth infections, Polymerase Chain Reaction</em></strong></p>

scholarly journals A View on Polymerase Chain Reaction as an Outstanding Molecular Diagnostic Technique in Periodontology

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Adileh Shirmohammadi ◽  
Amirreza Babaloo ◽  
Solmaz Maleki Dizaj ◽  
Farzaneh Lotfipour ◽  
Simin Sharifi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Periodontal Disease ◽  
English Language ◽  
Diagnostic Technique ◽  
Molecular Diagnostic ◽  
Molecular Technique ◽  
Chain Reaction ◽  
Effective Technique ◽  
Publication Time ◽  
Polymerase Chain

Objectives. This study presents a discussion on the fundamentals of polymerase chain reaction (PCR) and its use as a diagnostic tool in periodontology. Materials and Methods. A computer-aided as well as hand-made search in PubMed and Scopus indexed journals (relevant to the topic) was done by keywords of molecular technique in periodontology, PCR, applications of PCR, and PCR in periodontics. Only the papers in the English language and outlining PCR and its association with periodontology were collected and utilized to provide a succinct review. There was no limitation for publication time. Results. The results of our search showed that PCR has turned into a standard in diagnosis in the field of periodontology. A variety of researches has demonstrated that its sensitive, and specific characteristics make it a quick and effective technique of recognition, identification, and quantification of microorganisms. Identification of various immunoinflammatory markers at the mRNA expression level as well as ascertaining gene-related polymorphisms can also be performed. Conclusions. The mechanisms of periodontal disease can further become clarified using PCR. Clinical Relevance. PCR as a diagnostic method can play a main part in the validation of the clinical diagnosis of periodontal disease indicating the reason, pathogenesis, clinical steps, progress, and prognosis of the disease.

Comparison between Quantitative Polymerase Chain Reaction and Sodium Nitrate Flotation Microscopy in Diagnosing Soil-Transmitted Helminth Infections

2021 ◽  
Author(s):  
Adam W. Bartlett ◽  
Rebecca Traub ◽  
Salvador Amaral ◽  
Sze Fui Hii ◽  
Naomi E. Clarke ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sodium Nitrate ◽  
Primary Schools ◽  
Quantitative Polymerase Chain Reaction ◽  
Infection Intensity ◽  
Diagnostic Technique ◽  
Chain Reaction ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Polymerase Chain

There is evolving interest in alternate microscopy techniques and quantitative polymerase chain reaction (qPCR) to evaluate soil-transmitted helminth (STH) burden. Using data from a cross-sectional survey of 540 schoolchildren across six primary schools in three municipalities of Timor-Leste, we compared the performance of microscopy using sodium nitrate flotation (SNF) and qPCR in determining STH prevalence and infection intensity. Prevalence by qPCR was higher than SNF for Ascaris lumbricoides (17.5% versus 11.2%), hookworm (8.3% versus 1.2%), and Trichuris trichiura (4.7% versus 1.6%). Agreement between SNF and qPCR was fair for hookworm (κ = 0.21) and moderate for A. lumbricoides (κ = 0.59) and T. trichiura (κ = 0.44). Nearly all infections were light intensity by SNF, whereas qPCR identified 36.1% as moderate or heavy infections using cycle threshold to eggs per gram conversion formulas. Quantitative polymerase chain reaction is a promising diagnostic technique, though further studies validating infection intensity correlates are required.

scholarly journals A rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction

1999 ◽  
Vol 19 (2) ◽  
pp. 63-67 ◽  
Author(s):  
Ester T. González ◽  
Junzo Norimine ◽  
Alejandro R. Valera ◽  
Gabriel Travería ◽  
Graciela A. Oliva ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Viral Genome ◽  
Diagnostic Technique ◽  
Chain Reaction ◽  
Agar Gel ◽  
Bovine Leukaemia Virus ◽  
Leukaemia Virus ◽  
Routine Work ◽  
Polymerase Chain ◽  
Bovine Leukosis

Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). In Argentina, where a program to eradicate EBL has been introduced, sensitive and reliable diagnosis has attained high priority. Although the importance of the agar gel immunodiffusion test remains unchanged for routine work, an additional diagnostic technique is necessary to confirm cases of sera with equivocal results or of calves carrying maternal antibodies.Utilizing a nested shuttle polymerase chain reaction, the proviral DNA was detected from cows experimentally infected with as little as 5 ml of whole blood from BLV seropositive cows that were nonetheless normal in haematological terms. It proved to be a very sensitive technique, since it rapidly revealed the presence of the provirus, frequently at 2 weeks postinoculation and using a two-round procedure of nested PCR taking only 3 hours. Additionally, the primers used flanked a portion of the viral genome often employed to differentiate BLV type applying BamHI digestion. It is concluded that this method might offer a highly promising diagnostic tool for BLV infection.

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of the Glanzmann's Thrombasthenia Mutations in Arab and Iraqi-Jewish Patients by Polymerase Chain Reaction and Restriction Analysis of Blood or Urine Samples

1991 ◽  
Vol 66 (04) ◽  
pp. 500-504 ◽  
Author(s):  
H Peretz ◽  
U Seligsohn ◽  
E Zwang ◽  
B S Coller ◽  
P J Newman
Keyword(s):  
Polymerase Chain Reaction ◽  
Base Pair ◽  
Restriction Analysis ◽  
Urine Samples ◽  
Chain Reaction ◽  
Base Pairs ◽  
Glanzmann’S Thrombasthenia ◽  
Glanzmann's Thrombasthenia ◽  
Base Pair Deletion ◽  
Polymerase Chain

SummarySevere Glanzmann's thrombasthenia is relatively frequent in Iraqi-Jews and Arabs residing in Israel. We have recently described the mutations responsible for the disease in Iraqi-Jews – an 11 base pair deletion in exon 12 of the glycoprotein IIIa gene, and in Arabs – a 13 base pair deletion at the AG acceptor splice site of exon 4 on the glycoprotein IIb gene. In this communication we show that the Iraqi-Jewish mutation can be identified directly by polymerase chain reaction and gel electrophoresis. With specially designed oligonucleotide primers encompassing the mutation site, an 80 base pair segment amplified in healthy controls was clearly distinguished from the 69 base pair segment produced in patients. Patients from 11 unrelated Iraqi-Jewish families had the same mutation. The Arab mutation was identified by first amplifying a DNA segment consisting of 312 base pairs in controls and of 299 base pairs in patients, and then digestion by a restriction enzyme Stu-1, which recognizes a site that is absent in the mutant gene. In controls the 312 bp segment was digested into 235 and 77 bp fragments, while in patients there was no change in the size of the amplified 299 bp segment. The mutation was found in patients from 3 out of 5 unrelated Arab families. Both Iraqi-Jewish and Arab mutations were detectable in DNA extracted from blood and urine samples. The described simple methods of identifying the mutations should be useful for detection of the numerous potential carriers among the affected kindreds and for prenatal diagnosis using DNA extracted from chorionic villi samples.

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