Effects of aqueous leaf extract of azadirachta indica on some haematological parameters and blood glucose level in female rats

Diabetes is a metabolic disease and plant derived products are used to combat this deadly disease. Plant is a diverse source of numerous therapeutic compounds which can be used to ameliorate diabetes. Leaves of Coccinia grandis (L.) (Family: Cucurbitaceae) is one of the leafy vegetable that is used for this purpose traditionally. It has been used against diabetes for a very long time. Our aims were to identify the hypoglycemic effect of extract of leaves of Coccinia grandis as well as to determine its safety profile so that we could use the plant material to improve the diabetic condition. Diabetes was induced in rats by intraperitoneal injection of alloxan at a dose of 150 mg/Kg bodyweight and ethanolic extract of leaves of Coccinia grandis was fed to the rats at a dose of 750 mg/kg. We measured blood glucose level, and safety profile by measuring SGOT, SGPT and creatinine level on diabetic and non-diabetic rats before and after administration of the extract. After measuring blood glucose level, it was found that the hypoglycemic efficacy was comparable to that of metformin (p> 0.05) which was given at a dose of 500 mg/kg. Safety profile were investigated by checking SGOT, SGPT and creatinine level. It was seen that both metformin and leaf extract of Coccinia grandis improved the pathological condition induced by diabetes. Furthermore, in healthy individual rats both metformin and leaf extract of Coccinia grandis did not significantly alter the normal physiological state. It might, therefore, be inferred that the extract of leaves of Coccinia grandis could be used as a good alternative therapy to treat diabetes.

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Effect of Guava Leaf Extract in Combination with Acarbose or Voglibose on Increased Blood Glucose Level in Sugar-loaded Normal Mice

Nippon Eiyo Shokuryo Gakkaishi ◽

10.4327/jsnfs.56.207 ◽

2003 ◽

Vol 56 (4) ◽

pp. 207-212 ◽

Cited By ~ 11

Author(s):

Yoriko Deguchi ◽

Kuniko Osada ◽

Masaaki Watanuki

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Leaf Extract ◽

Guava Leaf

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The Activity of Wungu Leaf (Graptophyllum pictum (L) Griff) Extract in Reducing Blood Glucose Level of Hyperglycemic Mice

Current Biochemistry ◽

10.29244/cb.1.2.83-88 ◽

2014 ◽

Vol 1 (2) ◽

pp. 83-88

Author(s):

Hayatul Rahmi ◽

I Made Artika ◽

Norman Razief Azwar ◽

Djarot Sasongko Hami Seno ◽

Waras Nurcholis

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Blood Glucose Level ◽

Ethyl Acetate ◽

Glucose Level ◽

Diethyl Ether ◽

Leaf Extract ◽

Ethyl Acetate Extract ◽

Leaf Extracts ◽

Potential Use

Wungu leaf (Graptophyllum pictum (L.) Griff) is a plant thought to have potential use in alleviating symptoms of diabetes mellitus. The purpose of the present study was to evaluate the activity of wungu leaf extracts in decreasing blood glucose level of alloxan (200 mg/kg BW)-induced hyperglycemic mice. Extracts of wungu leaf were obtained by macerating with ethanol and then partitioning the extract with diethyl ether, ethyl acetate, and butanol. Each extract obtained was used to treat hyperglycemic mice for 28 days. The results showed that wungu leaf extracts have the ability to decrease the blood glucose level of hyperglycemic mice (dose 50 mg/kg BW). The ethyl acetate extract showed the highest activity, bringing about a decrease of blood glucose of 37.6 %. The wungu leaf extract has the potential to be developed as a source of anti-diabetic agents.

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PENGARUH PEMBERIAN EKSTRAK BUNGA DAN DAUN PEPAYA (Carica papaya L.) TERHADAP KADAR GLUKOSA DARAH TIKUS WISTAR (Rattus norvegicus L.) YANG HIPERGLIKEMIK

JURNAL ILMIAH SAINS ◽

10.35799/jis.17.2.2017.17681 ◽

2017 ◽

Vol 17 (2) ◽

pp. 143

Author(s):

Frendy Grenaldy Tangkumahat ◽

Johnly A Rorong ◽

Feti Ftimah

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Leaf Extract ◽

Carica Papaya ◽

Control Group ◽

Glucose Levels ◽

Flower Extract

PENGARUH PEMBERIAN EKSTRAK BUNGA DAN DAUN PEPAYA (Carica papaya L.) TERHADAP KADAR GLUKOSA DARAH TIKUS WISTAR (Rattus norvegicus L.) YANG HIPERGLIKEMIKABSTRAKTelah dilakukan penelitian tentang Pengaruh Pemberian Ekstrak Bunga dan Daun Pepaya (Carica papaya L.) terhadap Kadar Glukosa Darah Tikus Wistar (Rattus norvegicus L.) yang hiperglikemik. Tujuan penelitian ini untuk mengetahui pengaruh ekstrak bunga dan daun pepaya terhadap kadar glukosa darah tikus wistar. Penelitian ini menggunakan metode uji toleransi glukosa. Digunakan 30 ekor tikus wistar jantan yang dibagi dalam 5 kelompok perlakuan yaitu kelompok kontrol positif (K+) diberi Air, kelompok kontrol negatif (K-) diberi Sukrosa, Ekstrak bunga pepaya dosis 150 mg/kg BB (B1), Ekstrak bunga pepaya dosis 260 mg/kg BB (B2), Ekstrak daun pepaya dosis 100 mg/kg BB (D1) dan Ekstrak daun pepaya dosis 170 mg/kg BB (D2). Data diperoleh dari pemeriksaan kadar glukosa darah puasa setelah tikus diinduksi aloksan secara intraperitional dan setelah masa adaptasi selama 7 hari dan masa perlakuan selama 3 hari setelah diinduksi sediaan per oral (p.o.). Pemberian ekstrak Ethanol bunga dan daun pepaya (Carica Papaya L.) berpengaruh terhadap penurunan kadar glukosa darah tikus wistar (Ratus Norvegicus L.) yang Hiperglikemik. Dosis ekstrak bunga dan daun pepaya yang efektif dalam menurunkan kadar glukosa darah tikus yang hiperglikemia akibat diinduksi dengan aloksan adalah 260 mg/Kg BB dan dosis ekstrak daun 170 mg/Kg BB atau setara dengan bunga dan daun pepaya basah 200 g.Kata Kunci : Carica papaya L, Kadar Glukosa Darah, Aloksan, Hiperglikemik. EFFECT OF FLOWER AND PAPAYA LEAF EXTRACT (Carica papaya L.) ON WISTAR RATS BLOOD GLUCOSE LEVEL (Rattus norvegicus L.) WHICH HYPERGLYCEMIC ABSTRACTA Research had been carried out to determine the Effect of Flower and Papaya Leaf Extract (Carica papaya L.) on Wistar Rats Blood Glucose Level (Rattus norvegicus L.) which hyperglycemic. The purpose of this research is to know the effect of flower and papaya leaf extract on wistar rats blood glucose level. This research used glucose tolerance test method. Used 30 male wistar rats divided into 5 groups of treatment that is positive control group (K+) given Water, negative control group (K-) given Sucrose, papaya flower extract dose 150 mg/kg BW (B1), papaya flower extract dose 260 mg/kg BW (B2), papaya leaf extract dose 100 mg/kg BW (D1) and papaya leaf extract dose 170 mg/kg BW (D2). Data were obtained from fasting blood glucose examination after rats induced alloxan intraperitional and after a 7-day adaptation period and a 3-day treatment period after induced oral preparation (p.o.). The giving of Ethanol flower extract and papaya leaf (Carica Papaya L.) had an effect on the decrease of blood glucose level of Wistar rats (Ratus norvegicus L.) which Hyperglycemic. The dose of flower and papaya leaf extract is effective in lowering blood glucose levels of hyperglycemic rats induced by alloxan is dose of papaya extract 260 mg/Kg BW and dose of leaf extract 170 mg/kg BW or equal of 200g wet papaya flowers and leaves.Keywords: Carica Papaya L, Blood Glucose Levels, Alloxan, Hyperglycemic.

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Opposite Effects of F1 and F5 Fractions of Total Methanol Leaf-extract of Dialium guineense (Cesalpiniaceae) on Blood Glucose in Rat

Journal of Advances in Medical and Pharmaceutical Sciences ◽

10.9734/jamps/2020/v22i230154 ◽

2020 ◽

pp. 1-8

Author(s):

Firmin Sylva Barboza ◽

Madièye Sène ◽

Dominique Doupa ◽

Malick Ba ◽

Alassane Wélé ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Leaf Extract ◽

Methanol Extract ◽

Gel Permeation Chromatography ◽

Tolerance Test ◽

Diabetic Rats ◽

Type 2 Diabetic

The purpose of this study is to investigate the effects of total methanol leaf-extract of Dialium guineense (Cesalpiniaceae) and its fractions on blood glucose in rat. The fractions of methanol extract were obtained by chromatography Sephadex LH 20 gel, numbered F1 to F5. Experiments were performed in normoglycemic, glucose tolerance test, and type 2 diabetic rats. The total methanol leaf-extract (300 mg/kg, per os), induced a significant increase of blood glucose level in normoglycemic rats (2.27±0.12 vs 0.94±0.03 g/L) (p<0.05, n=5). F1 and F2 fractions (100 mg/kg, per os) increased the blood glucose level. Glycaemia respectively varied from 0.90 ± 0.03 to 2. 28 ± 0.22 g/L and 0.91 ± 0.03 to 1.43 ± 0.04 g /L (p<0.05, n=5). However, F5 fraction (300 mg/kg, per os), induced hypoglycemia (0.61 ± 0.01 vs 0. 80 ± 0.03 g/L) (p<0.05, n=5). F5 fraction prevented the pic of hyperglycemia caused by glucose (4 g/kg, per os). In type 2 diabetic rats, the daily oral administration of F5 fraction (300 mg/kg) induced an anti-hyperglycemic effect (1.28 ± 0.15 vs 4.48 ± 0.08 g/L). Fractionation by gel permeation chromatography allowed to highlight the presence of compounds both hyper-and hypoglycemic in total methanol leaf-extract of D. guineense. The lack of hypoglycemic effect in normoglycemic rats of total methanol leaf-extract, could respectively be related to the presence of both hyper- and hypoglycemic compounds in F1 and F5 fractions, which induced opposite effects.

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