scholarly journals Comparison of hematoxylin and eosin stain with modified gallego's stain for differentiating mineralized components in ossifying fibroma, cemento-ossifying fibroma, and cementifying fibroma

2019 ◽  
Vol 7 (3) ◽  
pp. 124
Author(s):  
Sandhya Tamgadge ◽  
Snehal Dhouskar ◽  
Avinash Tamgadge ◽  
Treville Periera ◽  
Uma Mudaliar ◽  
...  
Keyword(s):  
Ossifying Fibroma ◽  
Cementifying Fibroma ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain

Histologic Evaluation of Osseous Regeneration Following Combination Therapy With Platelet-Rich Plasma and Bio-Oss in a Rat Calvarial Critical-Size Defect Model

2015 ◽  
Vol 41 (5) ◽  
pp. 543-549 ◽  
Author(s):  
Philip J. DeNicolo ◽  
M. Kelly Guyton ◽  
Michael F. Cuenin ◽  
Steven D. Hokett ◽  
Mohamed Sharawy ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Growth ◽  
Critical Size ◽  
Close Contact ◽  
Platelet Rich Plasma ◽  
Critical Size Defect ◽  
Histologic Evaluation ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain ◽  
Osseous Regeneration

Platelet-rich plasma (PRP) is an autogenous source of growth factors shown to facilitate human bone growth. Bio-Oss, an osteoconductive xenograft, is used clinically to regenerate periodontal defects, restore dental alveolar ridges, and facilitate sinus-lift procedures. The purpose of this study was to analyze whether a combination of PRP and Bio-Oss would enhance bone regeneration better than either material alone. PRP and/or Bio-Oss were administered in an 8-mm critical-size defect (CSD) rat calvarial model of bone defect between 2 polytetrafluoroethylene membranes to prevent soft tissue incursion. Eight weeks after the induction of the CSD, histologic sections were stained with hematoxylin and eosin stain and analyzed via light microscopy. Qualitative analyses revealed new bone regeneration in all 4 groups. The Bio-Oss and PRP plus Bio-Oss groups demonstrated greater areas of closure in the defects than the control or PRP-only groups because of the space-maintaining ability of Bio-Oss. The groups grafted with Bio-Oss showed close contact with new bone growth throughout the defects, suggesting a stronger graft. The use of PRP alone or in combination with Bio-Oss, however, did not appear to enhance osseous regeneration at 8 weeks. Areas grafted with Bio-Oss demonstrated greater space-maintaining capacity than controls, and PRP was an effective vehicle for placement of the Bio-Oss. However, at 8 weeks this study was unable to demonstrate a significant advantage of using PRP plus Bio-Oss over using Bio-Oss alone.

scholarly journals Role of S-100 Immunostain as An Auxiliary Diagnostic Aid in Leprosy

2017 ◽  
Vol 56 (205) ◽  
pp. 141-144 ◽  
Author(s):  
Ramesh Dhakhwa ◽  
Sneh Acharya ◽  
Sailesh Pradhan ◽  
Sanju Babu Shrestha ◽  
Tomoo Itoh
Keyword(s):  
Erythema Nodosum ◽  
Lupus Vulgaris ◽  
Common Pattern ◽  
Tissue Sections ◽  
Histopathologic Diagnosis ◽  
S 100 ◽  
Skin Biopsies ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain

Introduction: Histopathologic diagnosis of leprosy is difficult when Bacillary Index (BI) is zero and neural involvement are not easily identifiable on routine Hematoxylin and Eosin stain. This study was undertaken to study the role of S-100 immunostaining in demonstrating different patterns of nerve involvement in various types of leprosy. Methods: Thirty one skin biopsies with clinico-histopathologic diagnoses of leprosy over a period of two years were included in the study. Ten cases of non-lepromatous granulomatous dermatoses (including eight cases of lupus vulgaris and two cases of erythema nodosum) were used as controls. Tissue sections from all cases and controls were stained with Hematoxylin and Eosin (H&E) stain, Fite stain and S-100 immunostain. The H&E stained slides were used to study the histopathological features, Fite stained slides for Bacillary Index and S-100 for nerve changes. Results: Neural changes could be demonstrated in the entire spectrum of leprosy using S-100 immunostaining. The most common pattern of nerve destruction in the tuberculoid spectrum was fragmented and infiltrated whereas lepromatous spectrum showed mostly fragmented nerve twigs. Intact nerves were not detected in any of the leprosy cases. Conclusions:  S-100 immunostain is a useful auxiliary aid to the routine  H&E stain in the diagnosis of leprosy especially tuberculoid spectrum and intermediate leprosy.  Keywords: bacillary index; leprosy; S-100 immunostain.

scholarly journals Mucormycosis of the hard palate masquerading as carcinoma

2012 ◽  
Vol 2 (1) ◽  
pp. 28 ◽  
Author(s):  
Bhari Sharanesha Manjunatha ◽  
Nagarajappa Das ◽  
Rakesh V. Sutariya ◽  
Tanveer Ahmed
Keyword(s):  
Clinical Presentation ◽  
Fungal Infections ◽  
Survival Rates ◽  
Positive Culture ◽  
Staining Technique ◽  
Necrotic Bone ◽  
Chronic Ulcers ◽  
Hematoxylin And Eosin ◽  
Clinical Differential Diagnosis ◽  
Hematoxylin And Eosin Stain

A growing number of medically compromised patients are encountered by dentists in their practices. Opportunistic fungal infections such as mucormycosis usually occur in immunocompromised patients but can infect healthy individuals as well. Mucormycosis is an acute opportunistic, uncommon, frequently fatal fungal infection, caused by a saprophytic fungus that belongs to the class of phycomycetes. Among the clinical differential diagnosis we can consider squamous cell carcinoma. Such cases present as chronic ulcers with raised margins causing exposure of underlying bone. There is a close histopathological resemblance between mucormycosis and aspergillosis. Microscopically, aspergillosis has septate branching hyphae, which can be distinguished from mucormycotic hyphae by a smaller width and prominent acute angulations of branching hyphae. A definitive diagnosis of mucormycosis can be made by tissue biopsy that identifies the characteristic hyphae, by positive culture or both. The culture of diseased tissue may be negative and histopathologic examination is essential for early diagnosis. Mucormycosis was long regarded as a fatal infection with poor prognosis. However with early medical and surgical management survival rates are now thought to exceed 80%. In the present case, the fungus was identified by hematoxylin and eosin stain and confirmed by Grocott’s silver methenamine special staining technique. Removal of the necrotic bone, which acted as a nidus of infection, was done. Post-operatively patient was advised an obturator to prevent oronasal regurgitation. Since mucormycosis occurs infrequently, it may pose a diagnostic and therapeutic dilemma for those who are not familiar with its clinical presentation.

Crooked Calf Disease: A Histological and Histochemical Examination of Eight Affected Calves

1986 ◽  
Vol 23 (6) ◽  
pp. 734-740 ◽  
Author(s):  
L. C. Abbott ◽  
R. H. Finnell ◽  
G. F. Chernoff ◽  
S. M. Parish ◽  
C. C. Gay
Keyword(s):  
Congenital Anomalies ◽  
Adenosine Triphosphatase ◽  
Normal Muscle ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain ◽  
Histochemical Examination

Macroscopic, histopathologic, and histochemical investigations were made on a group of eight neonatal Angus × Hereford calves, selected from an ongoing outbreak of crooked calf disease among calving heifers. Arthrogryposis of the forelimbs was seen to varying degrees in all eight animals, and torticollis was present in six calves. Histopathology, using hematoxylin and eosin stain, did not reveal any striking or consistent lesion in the affected animals; the majority of the tissues sampled were normal. Muscle samples were processed for adenosine triphosphatase (ATPase) and NADH-tetrazolium reductase (NADH-tr) histochemistry, and the data suggest that a primary myopathy is not responsible for the congenital anomalies in the affected calves.

scholarly journals Histopathological study of endometrium in abnormal uterine bleeding

2012 ◽  
Vol 2 (4) ◽  
pp. 297-300 ◽  
Author(s):  
S Bhatta ◽  
AK Sinha
Keyword(s):  
Histopathological Examination ◽  
Abnormal Uterine Bleeding ◽  
Uterine Bleeding ◽  
Common Finding ◽  
Histopathological Study ◽  
Endometrial Sampling ◽  
Proliferative Endometrium ◽  
Malignant Lesions ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain

Background: Histopathological evaluation of endometrial samples is essential in the workup of abnormal uterine bleeding. This study was carried out to evaluate patterns of endometrial histological findings in women with abnormal uterine bleeding and to correlate histopathological findings with clinical features.Materials and Methods: One hundred and twenty two patients with diagnosis of abnormal uterine bleeding underwent endometrial sampling. The slides stained with Hematoxylin and Eosin stain were studied.Results: The predominant endometrial histopathological finding was proliferative endometrium 32 cases (26.23%) followed by simple hyperplasia without atypia 22 cases (18.03%). Malignant lesions were more common in patients more than 40 years of age and comprised of 7 cases (5.74%) of all cases. Atrophic endometrium was most common finding in postmenopausal bleeding 8 cases (28.6%) followed by endometrial carcinoma 5 cases (17.9%).Conclusion: Histopathological examination of endometrium should be done generously in women presenting with abnormal uterine bleeding especially after the age of 40 years to rule out malignant pathology.Journal of Pathology of Nepal (2012) Vol. 2, 297-300DOI: http://dx.doi.org/10.3126/jpn.v2i4.6882

scholarly journals Histopathological Study of Endometritis of the cows

2010 ◽  
Vol 9 (1) ◽  
pp. 69 ◽  
Author(s):  
A. Gh. Rhyaf
Keyword(s):  
Light Microscope ◽  
Inflammatory Cells ◽  
Histopathological Study ◽  
Epithelial Layer ◽  
Histopathological Changes ◽  
Uterine Tissue ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain ◽  
Uterine Glands

The present study was designed to investigate histopathological changes of endometritis in cows. 32 specimens of uteri of normal cows were collected from AL-Diwanyia slaughterhouse during 3 months twice weekly. All these specimens were cut and prepared for histopathological sections, then stained with hematoxylin and eosin stain and it examined under a light microscope, the result showed hyperplasia of the epithelial layer of the uterus, severe infiltration of inflammatory cells, atrophy of uterine glands with hemorrhage and serious edema in the uterine tissue in some cases.

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