Chemical composition of Moringa oleifera ethyl acetate fraction and its biological activity in diabetic human dermal fibroblasts

Abstract Background Diabetes mellitus is a metabolic disorder of glucose metabolism and management of blood glucose level is the hallmark in the treatment of this disease. The present study investigated chemical composition, in vitro antioxidant and antidiabetic activity of different fractions of 80% methanol Piper guineense leaves extract. Materials and methods The crude methanolic extract of P. guineense was obtained following 80% methanol cold extraction and was successively partitioned with dichloromethane (DCM), ethyl acetate (EtOAc), n-butanol (nBuOH) and aqueous solvents to give four fractions. The chemical composition of the fractions from P. guineense was determined using gas chromatography coupled with mass spectrometry (GC-MS) and their potentials as antioxidant and anti-diabetes were evaluated. Results The percentage yields were 3.16, 2.22, 0.68 and 0.66% (w/w) in n-butanol, DCM, aqueous and ethyl acetate fractions of P. guineense methanolic extract, respectively. The GC-MS analyses identified a total of 71 and 34 phytochemicals in n-butanol and ethyl acetate fractions respectively. Tributyl acetylcitrate (10.95%) and phytol (9.11%) were the major components in the n-butanol fraction while ethyl piperonyl cyanoacetate (27.35%) and phytol (15.17%) were the major constituents in the ethyl acetate fraction. Ethyl acetate fraction had the highest ferric reducing antioxidant power with a value of 53.96 ± 0.40 mgAAE/g while n-butanol fraction possessed highest total antioxidant power (9.98 ± 0.15) followed by aqueous fraction (9.72 ± 0.02). The ethyl acetate and n-butanol fractions with IC50 value of 0.24 ± 0.07 and 0.83 ± 0.15 μg/mL respectively elicited significant inhibitory activities against α-glucosidase while only n-butanol fraction (IC50 = 0.33 ± 0.09 μg/ml) exhibited appreciable inhibition against α-amylase activity. However, none of the four fractions showed significant inhibitory activity towards dipeptidyl-peptidase-IV. Conclusion n-butanol and ethyl acetate fractions of 80% methanol P. guineense leaves extract can be a potential source of bioactive compounds of pharmacological importance in the management of diabetes.

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The effect of ethyl acetate fraction of Moringa oleifera leaves on neutrophil and MDA levels in the improvement of liver dysfunction in male rats with sepsis model

Bali Medical Journal ◽

10.15562/bmj.v9i3.1850 ◽

2020 ◽

Vol 9 (3) ◽

pp. 721

Author(s):

Riswanto Riswanto ◽

Tatar Sumandjar ◽

Dhani Redhono ◽

Robby Kurniawan ◽

Arief Rahman

Keyword(s):

Ethyl Acetate ◽

Liver Dysfunction ◽

Moringa Oleifera ◽

Ethyl Acetate Fraction ◽

Male Rats ◽

Sepsis Model ◽

Moringa Oleifera Leaves

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PHYTOCHEMICAL SCREENING, TOTAL PHENOLIC CONTENT, AND ANTIOXIDANT ACTIVITY OF WATER, ETHYL ACETATE, AND N-HEXANE FRACTIONS FROM MISTLETOE MORINGA OLEIFERA LAM. (DENDROPHTHOE PENTANDRA (L.) MIQ.)

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i10.25271 ◽

2018 ◽

Vol 11 (10) ◽

pp. 104

Author(s):

Nia Kristiningrum ◽

Lestyo Wulandari ◽

Aini Zuhriyah

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Total Phenolic Content ◽

Moringa Oleifera ◽

Phenolic Content ◽

Ethyl Acetate Fraction ◽

Total Phenolic ◽

Phytochemical Screening ◽

Significance Level ◽

Water Fraction

Objective: This study was to investigate the phytochemical screening, total phenolic content (TPC), and antioxidant activity of fractions of water, ethyl acetate, and n-hexane from Dendrophthoe pentandra (L.) Miq. and mistletoe of Moringa oleifera Lam.Methods: Simplicia extracted with 96% ethanol using maceration method. Liquid-liquid extraction was used for fractionation with water, ethyl acetate, and n-hexane as solvent. Phytochemical screening, determination of TPC, and antioxidant activity were performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method. The TPC was measured by Folin–Ciocalteu reagent and use gallic acid (GA) as a standard.Results: Water fraction contains saponin and polyphenols. Ethyl acetate fraction contains alkaloids, flavonoids, saponins, terpenoids, and polyphenols. N-hexane fraction only contains alkaloids and terpenoids. The TPC and antioxidant activity of fractions of water, ethyl acetate, and n-hexane were 9.42±1.16%, 21.35±1.86%, and 67.40±0.82% mg GA equivalent/g fraction and 29.46 μg/ml±0.99%, 7.08 μg/ml±0.39%, and 10.90 μg/ml±1.05%, respectively.Conclusion: Fraction of ethyl acetate exhibited the highest antioxidant activity and TPC. This study showed that there were significant correlations between TPC and antioxidant activity at significance level p<0.05.

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BIOLOGICAL ACTIVITY OF EXTRACTS OF PLANT HAPLOPHYLLUM PERFORATUM

EurasianUnionScientists ◽

10.31618/esu.2413-9335.2020.2.79.1041 ◽

2020 ◽

Vol 2 (10(79)) ◽

pp. 76-79

Author(s):

U. Mamarozikov ◽

S. Turaeva ◽

R. Zakirova ◽

N. Khidirova

Keyword(s):

Biological Activity ◽

Ethyl Acetate ◽

High Activity ◽

Fungicidal Activity ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Chloroform Fraction ◽

Microscopic Fungus ◽

Haplophyllum Perforatum ◽

Water Alcohol

The article presents the results of studies of the fungicidal and growth-stimulating activity of various parts of the ethanol extract of the plant H. perforatum. As a result of fractionation of the extract, a hexane fraction, a chloroform fraction, an ethyl acetate fraction, and a water-alcohol residue were obtained. The ethanol extract showed a high fungicidal activity against the microscopic fungus Fusarium oxisporum. The study of the growthstimulating effect on the culture of cucumbers showed a high activity of the hexane part of the extract.

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Characterization of Chemical Composition of Bryophyllum pinnatum Leaf Ethyl Acetate Fraction

Asian Journal of Advanced Research and Reports ◽

10.9734/ajarr/2021/v15i430387 ◽

2021 ◽

pp. 15-24

Author(s):

E. S. Asiwe ◽

C. U. Igwe ◽

V. A. Onwuliri ◽

K. M. E. Iheanacho ◽

J. N. Iheanacho

Keyword(s):

Gas Chromatography ◽

Chemical Composition ◽

Methyl Ester ◽

Ethyl Acetate ◽

Acid Methyl Ester ◽

Ethyl Acetate Fraction ◽

Potential Candidate ◽

Acid Methyl ◽

Ms Analysis ◽

Bryophyllum Pinnatum

Aims: To characterize the chemical composition of Bryophyllum pinnatum leaf ethyl acetate fraction. Methodology: Quantitative phytochemical composition was assessed using gas chromatography fitted with flame ionization detector (GC-FID), while chemical characterization was via gas chromatography-mass spectrophotometry (GC-MS) analysis. The mass spectra peaks were matched with those found in the National Institute of Standard and Technology (NIST) spectral database. Results: Results revealed a rich presence of proanthocyanin, rutin, quinine, flavan-3-ol, anthocyanin, lunamarin, sapogenin, phenol, flavonones, steroids, epicatechin, kaempferol, phytate, oxalate, resveratol, catechin, flavones, tannin, ribalinidine, naringin, and spartein in varying proportion in B. pinnatum leaves ethylacetate fractions. GC-MS analysis of the fractions revealed the presence of at least 50 constituents. The major constituents were Hexadecanoic acid, methyl ester (24.88%), 10,13-Octadecadienoic acid, methyl ester (29.69%), Tetracosanoic acid, methyl ester (7.84%), Methyl stearate (6.97%), cis-Methyl 11-eicosenoate (6.26%), Methyl 18-methylnonadecanoate (4.99%), Docosanoic acid, methyl ester (3.71%) and 4,7-Methano-1H-indene, octahydro- (2.43%). Conclusion: This rich array of chemical compounds presents B. pinnatum leaves as an important source of potential lead compounds with biological and pharmacological benefits and hence a potential candidate for drug discovery.

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