scholarly journals Lactase production by Saccharomyces fragilis IZ 275 using different carbon sources

2020 ◽  
Vol 15 (3) ◽  
pp. 1
Author(s):  
Alessandra Bosso ◽  
Adriana Aparecida Bosso Tomal ◽  
Lucas Caldeirão Miranda ◽  
Josemeyre Bonifácio da Silva ◽  
Hélio Hiroshi Suguimoto ◽  
...  
Keyword(s):  
Central Composite Design ◽  
Factorial Design ◽  
Cheese Whey ◽  
Carbon Sources ◽  
Dairy Industry ◽  
Fermentation Medium ◽  
Maximum Activity ◽  
Efficient Tool ◽  
Additional Value ◽  
Central Composite

  This study sought to create a better fermentation medium to maximize lactase production by Saccharomyces fragilis IZ 275 using different carbon sources, including reconstituted powdered cheese whey. A factorial design 24 was applied to evaluate the significant effects of variables which compose the fermentation medium. Then, a steepest descent-ascent design was applied to obtain the maximum activity. A Rotational Central Composite Design (RCCD) 24 was made to optimize the fermentation medium. We verified that the cheese whey, a by-product of the dairy industry, can be employed as an excellent fermentation medium by yeast, within the bioeconomy concept and used by the dairy industry as product with additional value. The employed methodology is an efficient tool in the optimization process for β-galactosidase production. In the optimized fermentation medium, the maximum production of β-galactosidase (54.68 U/mL) by S. fragilis IZ 275 is obtained with 14 g/L sucrose, 17.7 g/L reconstituted powdered cheese whey, 5.14 g/L yeast extract and 8.85 g/L peptone.

Optimization of extracellular fungal peroxidase production by 2 Coprinus species

2004 ◽  
Vol 50 (12) ◽  
pp. 1033-1040 ◽  
Author(s):  
Keisuke Ikehata ◽  
Michael A Pickard ◽  
Ian D Buchanan ◽  
Daniel W Smith
Keyword(s):  
Central Composite Design ◽  
Factorial Design ◽  
Fractional Factorial Design ◽  
Culture Conditions ◽  
Production Optimization ◽  
Fractional Factorial ◽  
Coprinus Cinereus ◽  
Screening Study ◽  
Design Production ◽  
Central Composite

Optimum culture conditions for the batch production of extracellular peroxidase by Coprinus cinereus UAMH 4103 and Coprinus sp. UAMH 10067 were explored using 2 statistical experimental designs, including 2-level, 7-factor fractional factorial design and 2-factor central composite design. Of the 7 factors examined in the screening study, the concentrations of carbon (glucose) and nitrogen (peptone or casitone) sources showed significant effects on the peroxidase production by Coprinus sp. UAMH 10067. The optimum glucose and peptone concentrations were determined as 2.7% and 0.8% for Coprinus sp. UAMH 10067, and 2.9% and 1.4% for C. cinereus UAMH 4103, respectively. Under the optimized culture condition the maximum peroxidase activity achieved in this study was 34.5 U·mL–1 for Coprinus sp. UAMH 10067 and 68.0 U·mL–1 for C. cinereus UAMH 4103, more than 2-fold higher than the results of previous studies.Key words: Coprinus peroxidase, central composite design, fractional factorial design, production optimization, response surface.

scholarly journals OPTIMASI RESPON PADA PERCOBAAN FAKTORIAL DENGAN MENGGUNAKAN METODE PERMUKAAN RESPON

2019 ◽  
Vol 4 (2) ◽  
pp. 51
Author(s):  
Falin Tristanti Ayu ◽  
Izzati Rahmi HG ◽  
Yudiantri Asdi
Keyword(s):  
Response Surface Methodology ◽  
Central Composite Design ◽  
Factorial Design ◽  
Response Surface ◽  
Lack Of Fit ◽  
Central Composite

Metode Permukaan Respon atau Response Surface Methodology adalah gabungan dari teknik matematika dan statistika yang digunakan dalam pemodelan dan analisis dimana respon yang diamati dipengaruhi oleh sejumlah variabel. Metode permukaan respon digunakan untuk mencari taraf-taraf peubah bebas yang dapat mengoptimalkan respon. Dengan metode ini dapat diketahui model empirik yang menyatakan hubungan antara variabel-variabel independen dengan variabel respon, serta dapat diketahui nilai variabel-variabel independen yang menyebabkan nilai variabel respon menjadi optimal. Eksperimen dengan metode permukaan respon dilakukan dalam dua tahap yaitu eksperimen tahap I dan eksperimen tahap II. Desain eksperimen yang digunakan pada eksperimen tahap I adalah desain faktorial dua level sedangkan desain eksperimen yang digunakan pada eksperimen tahap II adalah Central Composite Design (CCD). Tahapan dalam metode permukaan respon pada intinya yaitu mencari fungsi aproksimasi yang menyatakan hubungan antara variabel independen dengan variabel respon, mengestimasi parameter-parameter dari fungsi aproksimasi yang diperoleh dengan metode kuadrat terkecil dan selanjutnya dilakukan analisis pengepasan permukaan. Karakteristik permukaan respon digunakan untuk menentukan apakah jenis titik stasionernya maksimum, minimum atau titik pelana. Prosedur pengujian yang dilakukan dalam metode permukaan respon diantaranya uji kesesuaian model regresi (lack of fit), uji parameter regresi secara serentak dan pengujian asumsi residual.Kata Kunci: Desain eksperimen, Metode Permukaan Respon (Response Surface Methodology), Two Level Factorial Design, Central Composite Design (CCD)

Riveting parameter design that satisfies requirements for driven rivet head dimensions

2014 ◽  
Vol 229 (13) ◽  
pp. 2412-2432 ◽  
Author(s):  
SF Wang ◽  
JH Zhang ◽  
ZG Liu ◽  
XW Zhang ◽  
J Hong ◽  
...  
Keyword(s):  
Central Composite Design ◽  
Factorial Design ◽  
Fractional Factorial Design ◽  
Initial Crack ◽  
Fractional Factorial ◽  
Feasible Region ◽  
Quality Requirements ◽  
Steepest Ascent ◽  
The Impact ◽  
Central Composite

Riveted joints are extensively adopted in designing aircraft structures. Riveting implies a squeezing process of the rivet with large plastic deformations to form the driven rivet head. The driven rivet head dimensions (height H, diameter D) depend on riveting force ( X1), rivet length and diameter tolerance ( X2 and X3), as well as rivet hole tolerance ( X4). Incorrect selection in these parameters could induce the excessive stress concentration that results in initial crack and also results in improper rivet head deformation leading to loose rivet. The present research is conducted on a MS2047AD6-6 rivet and 2.286 mm thick aluminum alloy sheets and mainly focuses on the design of riveting parameters X1, X2, X3, and X4 using the proposed three-step statistical experiment designs including fractional factorial design, steepest ascent design, and central composite design while satisfying the quality requirements for driven rivet head dimensions ( H, D) mentioned in Standard Aircraft Handbook. Fractional factorial design is used to evaluate the impact of riveting parameters X1, X2, X3, and X4 on H and D. Based on the effective ranges of the significant riveting parameters obtained from steepest ascent design, a five-level central composite design is proposed to derive the statistical relations between H, D and the significant riveting parameters, and the statistical models are used to find the feasible region resulting from the combination of the significant riveting parameters while satisfying the quality requirements for H and D. Finally, the feasible ranges of X1, X2, X3, and X4, namely [16,470 N 22,730 N], [−0.1491 mm 0.3891 mm], [−0.0466 mm 0.1216 mm], and [−0.0375 mm 0.2125 mm], are determined from the perspective of adjustable accuracy of X1 and that of the manufacturability for X2, X3, and X4. It implies that any combination of X1, X2, X3, and X4 that falls within this feasible region can result in a good quality riveted joins, namely that the quality requirements for the driven riveting head dimension ( H, D) can be satisfied.

scholarly journals OPTIMASI PERMUKAAN RESPON MEDIUM FERMENTASI Streptomyces prasinopilosus SEBAGAI ANTIFUNGI TERHADAP PATOGEN Ganoderma boninense

2019 ◽  
Vol 6 (2) ◽  
pp. 164
Author(s):  
Rofiq Sunaryanto ◽  
Diana Nurani
Keyword(s):  
Central Composite Design ◽  
Yeast Extract ◽  
Active Compound ◽  
Carbon Sources ◽  
Pathogenic Fungi ◽  
Medium Composition ◽  
Quadratic Model ◽  
Active Compounds ◽  
Area Unit ◽  
Central Composite

Response Surface Optimization of Medium Fermentation for Streptomyces prasinopilosus as An Antifungal against Ganoderma boninenseGanoderma boninense is one of the pathogenic fungi that cause basal stem rot (BPB) on oil palm plants. This research aims to study the effect of carbon sources, nitrogen sources, and minerals on the production of Streptomyces prasinopilosus active compounds. Lactose, yeast extract, and minerals are medium components that show a real influence on the production of S. prasinopilosus active compounds. Optimization of the factors that have significant influence was predicted by the second-order model, statistically through a central composite design (CCD). The highest S. prasinopilosus active compound production, with a medium composition of 44.77 g L-1 lactose, 13.02 g L-1 yeast extract, and 15.95 mL L-1 mineral solution, was predicted by the quadratic model to reach 32269366.338 peak area unit on high-performance liquid chromatography (HPLC). The verification of the mathematical model of the production of the active compounds through experiments in the laboratory was 27,203,907.310 peak area unit. This result was 15.7% lower compared to the result of the quadratic model. Optimization increased S. prasinopilosus active compound 9-fold compared to that before optimization.Keywords: active compound; G. boninense; optimization; RSM; S. prasinopilosus ABSTRAKGanoderma boninense merupakan salah satu jamur patogen yang menyebabkan penyakit busuk pangkal batang atau biasa disebut BPB pada tanaman kelapa sawit. Penelitian bertujuan mempelajari pengaruh sumber karbon, sumber nitrogen, dan mineral terhadap produksi senyawa aktif S. prasinopilosus. Laktosa, yeast extract, dan mineral adalah komponen medium yang menunjukkan pengaruh nyata terhadap produksi senyawa aktif S. prasinopilosus. Optimasi terhadap faktor yang berpengaruh nyata diprediksi dengan model orde dua melalui rancangan statistis central composite design (CCD). Produksi senyawa aktif S. prasinopilosus tertinggi diprediksi oleh model kuadratik mencapai 32269366,338 luasan puncak kromatografi cair kinerja tinggi (KCKT) dengan komposisi medium laktosa 44,77 g L-1, yeast extract 13,02 g L-1, dan larutan mineral 15,95 mL L-1. Verifikasi model matematis produksi senyawa aktif yang dihasilkan melalui percobaan di laboratorium adalah sebesar 27.203.907,310 luasan puncak kromatogram KCKT. Hasil ini lebih rendah 15,7% dibandingkan dengan model kuadratik hasil optimasi. Optimasi meningkatkan senyawa aktif S. prasinopilosus 9 kali lipat dibandingkan sebelum optimasi.

Valorization of de-oiled cakes for xylanase production and optimization using central composite design by Trichoderma koeningi isolate

2017 ◽  
Vol 42 (3) ◽  
Author(s):  
Ramesh Bandikari ◽  
Umamahesh Katike ◽  
Naga Sivudu Seelam ◽  
Vijaya Sarathi Reddy Obulam
Keyword(s):  
Central Composite Design ◽  
18S Rrna ◽  
Fermentation Medium ◽  
Xylanase Production ◽  
Corn Cobs ◽  
Medium Components ◽  
The Media ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

AbstractAimEvaluation of the medium components and conditions for the optimization of xylanase production in submerged fermentation byMethodswas identified by the 18s rRNA. NaOH pre-treated corn cobs were used in order to reduce the crystallinity of corn cobs. De-oiled cakes proximate composition was analyzed according to AOAC. Plackett-Burman design (PBD) was used to screen the 19 media variables that affect xylanase production and optimized the media by central composite design (CCD).ResultsThe isolate was identified asConclusionNaOH pre-treated corn cobs were used with mustard cake powder as fermentation medium constituent to induce the xylanase production. From the results we conclude that mustard cake induced the xylanase production.

Determination of cocaine, its metabolites and pyrolytic products by LC-MS using a chemometric approach

2014 ◽  
Vol 6 (2) ◽  
pp. 456-462 ◽  
Author(s):  
Andrea Garcia Pereira ◽  
Felipe Bianchini D'Avila ◽  
Pâmela C. Lukasewicz Ferreira ◽  
Marcelo Gatteli Holler ◽  
Renata Pereira Limberger ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Central Composite Design ◽  
Factorial Design ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chemometric Approach ◽  
Pyrolytic Products ◽  
Face Centered ◽  
Central Composite

A method to assay cocaine, its metabolites, pyrolytic products and adulterant levamisole was developed and validated by liquid chromatography-mass spectrometry (LC-MS) using a chemometric approach including a two-level factorial design and face-centered central composite design (FCCCD) to achieve the optimization.

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