Variability of apparent ileal amino acid digestibility in different pea samples for growing-finishing pigs

1999 ◽  
Vol 79 (4) ◽  
pp. 467-475 ◽  
Author(s):  
M. Z. Fan ◽  
W. C. Sauer
Keyword(s):  
Amino Acid ◽  
Crude Protein ◽  
Corn Starch ◽  
Latin Square ◽  
Experimental Period ◽  
Trypsin Inhibitor Activity ◽  
Significant Finding ◽  
Finishing Pigs ◽  
Initial Body Weight ◽  
Ileal Digestibility

Six barrows, average initial body weight 32.5 kg, fitted with a simple T-can-nula at the distal ileum, were used to investigate factors responsible for the variability of apparent ileal amino acid (AA) digestibility values among six pea samples. The barrows were fed six corn-starch-based diets, formulated to contain 16.5% crude protein (CP) (as-fed) from six different pea samples, according to a 6 × 6 Latin square design. Chromic oxide (0.3%) was used as the digestibility marker. The pigs were fed twice daily, at 0800 and 2000 h. The dietary allowance was 1600 g−1. Each experimental period lasted 7 d. Ileal digesta were collected at 2-h intervals for a total of 24 h from 0800 h on day 6 to 0800 h on day 8. With the exception of arginine, cysteine, histidine, and methionine, there were differences (P < 0.05) in the apparent ileal digestibility values of the indispensable including semi-essential (+semi-) AA among the pea samples. Of the indispensable AA (+semi-) within each pea sample, the digestibility values of arginine and lysine were relatively high, ranging from 88.3 to 91.3% and from 78.7 to 85.2%, respectively, while the digestibility values of cysteine, methionine, and tryptophan were relatively low, ranging from 53.8 to 62.7%, from 69.4 to 75.4%, and from 53.1 to 70.4%, respectively. With the exception of arginine, cysteine, and tryptophan, the ileal digestibility values of the indispensable AA (+semi-) were negatively correlated (P < 0.05) with the neu-tral-detergent fiber content in the pea samples. Of all the AA, only the digestibility of tryptophan was negatively correlated (P < 0.05) with the trypsin inhibitor activity in the pea samples. The significant finding of this study was that with the exception of arginine, cysteine, and proline, differences in the digestibility values of all other AA in the pea samples were positively correlated (P < 0.05) with their respective dietary AA contents. This suggests that differences in AA levels in the assay diets were responsible for the variability of apparent ileal digestibility values of AA between pea samples. Therefore, to eliminate this methodological effect, true rather than apparent ileal AA digestibility values for peas should be determined. Key words: Amino acids, ileal digestibility, peas, pigs

Variability of apparent ileal amino acid digestibility in canola meal for growing-finishing pigs

1996 ◽  
Vol 76 (4) ◽  
pp. 563-569 ◽  
Author(s):  
M. Z. Fan ◽  
W. C. Sauer ◽  
V. M. Gabert
Keyword(s):  
Amino Acids ◽  
Crude Protein ◽  
Corn Starch ◽  
Latin Square ◽  
Experimental Period ◽  
Neutral Detergent Fiber ◽  
Canola Meal ◽  
Finishing Pigs ◽  
Analysis Method ◽  
Ileal Digestibility

Studies were conducted with six barrows, average initial body weigh 32.5 kg, fitted with a T-cannula at the distal ileum, to determine some of the factors responsible for the variability in apparent ileal digestibility values of amino acids (AA) in canola meal. The barrows were fed six corn starch-based diets, formulated to contain 18.0% crude protein (CP) on a dry matter (DM) basis from six canola meal samples, according to a 6 × 6 Latin square design. Chromic oxide (0.3%) was used as a digestibility marker. The pigs were fed twice daily, at 08:00 and 20:00 h. The dietary allowance was 1600 g d−1. Each experimental period consisted of 8 d. Ileal digesta were collected at 2-h intervals, for a total of 24 h from 08:00 on day 6 to 08:00 h on day 8. With the exception of proline, there were differences (P < 0.05) in the apparent ileal digestibility values of all AA among the six canola meal samples. Of the indispensable AA in canola meal, the digestibility values of arginine, histidine, and methionine were relatively high, ranging from 79.4 to 84.4, from 76.5 to 81.0%, and from 77.3 to 82.4%, respectively, while the digestibility values of threonine and tryptophan were relatively low, ranging from 59.7 to 66.5 and from 61.7 to 67.5%, respectively. Furthermore, with the exception of arginine, the digestibility values of the indispensable AA were negatively correlated (P < 0.05) with the neutral-detergent fiber (NDF) content in canola meal. In addition with the exception of threonine, tryptophan and valine, the digestibility values of the indispensable AA were positively correlated (P < 0.05) with their respective dietary levels. In conclusion, measured with the ileal analysis method, there were differences (P < 0.05) in the digestibility values of the majority of AA among the canola meal samples. Differences in NDF content were mainly responsible for the variation. In addition, differences in the dietary levels of most AA also contributed, in part, to the variation of AA digestibility values. Key words: Amino acids, ileal digestibility, canola meal, pigs

Effect of dietary fibre level on amino acid digestibility in young pigs

1994 ◽  
Vol 74 (2) ◽  
pp. 327-333 ◽  
Author(s):  
S. Li ◽  
W. C. Sauer ◽  
R. T. Hardin
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Dietary Fibre ◽  
Crude Protein ◽  
Corn Starch ◽  
Experimental Period ◽  
Period Change ◽  
Young Pigs ◽  
Average Body

Studies were carried out with 12 pigs (Yorkshire × Landrace) to determine the effect of dietary fibre level on amino acid digestibility. The pigs were weaned at 21 d of age and fitted with a simple T-cannula at the distal ileum at 23 or 24 d of age. The pigs were fed four isonitrogenous corn starch-based soybean meal diets (22.5 % crude protein) containing 4.3, 7.3, 10.3 and 13.3% Solkafloc according to a balanced two-period change-over design. The pigs were fed four times daily, equal amounts, at 6-h intervals. The diets were supplied at a rate of 5% of the average body weight which was determined at the initiation of the first (8.8 kg) and second (11.7 kg) experimental period. Each experimental period comprised 10 d. Feces were collected for 48 h on days 6 and 7 and ileal digesta for 24 h during days 8, 9 and 10. Chromic oxide was used as digestibility marker. The dietary inclusion of Solkafloc, up to 13.3 %, did not affect (P > 0.05) the apparent ileal as well as the fecal digestibilities of crude protein and amino acids. In conclusion, starter pigs can tolerate up to 13.3% Solkafloc, equivalent to 16.8% neutral-detergent fibre, in corn starch-based soybean meal diets without a depression in amino acid digestibility. Key words: Young pigs, fibre, amino acids, digestibility

The effect of dietary homoarginine derived from guanidination of synthetic lysine on endogenous amino acid loss and apparent and true ileal amino acid digestibility in the pig

2006 ◽  
Vol 82 (1) ◽  
pp. 23-30 ◽  
Author(s):  
H. L. Zhang ◽  
D. F. Li ◽  
S. Y. Qiao ◽  
F. L. Wang ◽  
X. J. Chen ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Crude Protein ◽  
Jugular Vein ◽  
Latin Square ◽  
Large White ◽  
Ileal Digestibility ◽  
Lysine Concentration ◽  
Single Meal ◽  
Endogenous Amino Acid

AbstractThese studies were conducted to determine whether dietary homoarginine, derived from guanidination of synthetic lysine, affects the measurement of apparent and true ileal amino acid digestibility in the pig and to determine the optimal ratio of o-methylisourea (OMIU) to free synthetic lysine in the guanidination reaction that produces homoarginine. In two preliminary experiments, specific amounts of lysine were weighed and added to a solution of 0·6 mol/l OMIU to produce OMIU to free synthetic lysine ratios of 2:1, 1·5:1, 1·4:1, 1·3:1, 1·2:1, 1·1:1 and 1·0:1. The mixture was incubated at a pH of 10·5 and a temperature of 20±1°C for 6 days. The highest yield of homoarginine (proportionately 0·995) was produced with an OMIU to free synthetic lysine ratio of 1·5:1. The serum concentrations of homoarginine and lysine were measured in two test pigs at various times after consuming a single meal containing either homoarginine or lysine. Blood samples were taken before and 0·5, 1·5, 2·5, 4·5, 6·0, 7·5, and 11·5 h after feeding. Thirty minutes after feeding the homoarginine-containing diet, the homoarginine concentration in serum was 0·013 mmol/dl, and increased to a maximum of 0·031 mmol/dl 1·5 h after feeding. Thirty minutes after feeding the lysine-containing diet, the serum lysine concentration was 0·011 mmol/dl and also reached its maximum concentration of 0·025 mmol/dl 1·5 h after feeding. The timing of the changes in concentration of homoarginine and lysine related to feeding were similar, which suggests that homoarginine is absorbed and metabolized in a similar manner to lysine. Six Large White×Landrace barrows, weighing 68·5±3·5 kg and fitted with a simple T-cannula at the distal ileum as well as a jugular vein catheter, were used to determine the effects of homoarginine on endogenous amino acid flows and apparent and true ileal amino acid digestibility. The experimental design was a doubly replicated 3×3 Latin-square design with the three treatments consisting of a lysine-free, zein-based diet and jugular infusion of a lysine solution; a lysine-free, zein-based diet supplemented with homoarginine; and a lysine-free, zein-based diet supplemented with l-lysine. There were no significant differences in endogenous ileal lysine flow for pigs receiving the different treatments (396·6, 407·6, and 360·4 mg/kg DMI, respectively, P=0·71). In addition, with the exception of phenylalanine (P=0·04), there were no significant differences (P>0·10) in the apparent and true ileal digestibility of crude protein and amino acids among the three treatments. Therefore, it would appear that the presence of dietary homoarginine, derived from guanidination of synthetic lysine, does not affect endogenous amino acid losses or the digestibility of crude protein as well as most amino acids. These results are therefore supportive of the use of the homoarginine method as a means of measuring true ileal lysine digestibility.

Effect of including purified jack bean lectin in casein or hydrolysed casein-based diets on apparent and true ileal amino acid digestibility in the growing pig

2004 ◽  
Vol 79 (2) ◽  
pp. 283-291 ◽  
Author(s):  
Y. -L. Yin ◽  
R. L. Huang ◽  
A. J. Libao-Mercado ◽  
E. A. Jeaurond ◽  
C. F. M. de Lange ◽  
...  
Keyword(s):  
Amino Acid ◽  
Latin Square ◽  
Live Weight ◽  
Experimental Period ◽  
Growing Pigs ◽  
Interactive Effects ◽  
Jack Bean ◽  
Ileal Digestibility ◽  
Growing Pig ◽  
Hydrolysed Casein

AbstractThe effect of including purified jack bean lectin (concanavalin A) in the diet of grower pigs on endogenous ileal nitrogen (N) and amino acid (AA) losses, and apparent and true ileal AA digestibility was investigated in a study with four growing pigs (average initial live weight (LW) 25 kg) fitted with simple T-cannulae at the terminal ileum. Both the homo-arginine (HA) method and peptide-alimentation method were used to estimate endogenous gut AA losses. The animals were offered four diets :(1) an enzymatically hydrolysed casein (EHC) containing diet, (2) an EHC containing diet supplemented with 266 mg/kg jack bean lectin, (3) a normal casein (NC) containing diet, and (4) a NC containing diet supplemented with 266 mg/kg lectin. For the estimation of ileal endogenous AA flows in pigs given NC diets, a subsample ofNC was guanidinated to convert lysine to HA. The experiment was conducted using a 4X4 Latin-square design. In each experimental period, pigs were adjusted to the experimental diets for 5 days. On day 6, ileal digesta was collected continuously for 24 hfor determination of apparent ileal digestibility. At 08:30 h of day 8 the HA NC diets were offered to pigs on NC treatments and ileal digesta were again collected for 24 h from all pigs. There were no interactive effects of casein type and added dietary lectin on apparent and true ileal AA digestibility and endogenous ileal AA flows (P > 0-05). The addition of lectin to the diet did not influence (P > 0-05) the apparent ileal digestibility of dry matter (DM), crude protein (CP) and AA. Casein type did not affect ileal DM digestibility (P > 0-05). Pigs given the NC-based diets had higher (P < 0-05) apparent ileal AA digestibility than pigs given the EHC-based diets. Addition of lectin to the diet did not influence (P > 0-05) endogenous ileal CP and AA flows. Endogenous ileal AA losses were generally higher (P < 0-05) in pigs given EHC diets than in pigs given NC diets. Neither casein type nor diet lectin level influenced (P > 0-05) the true ileal digestibility ofCP and AA; values ranged for most AA between 0-98 and 1-00. These data suggest that including purified jack bean lectin at the level of 266 mg/kg in a casein-based diet did not influence the ileal endogenous CP and AAflow, nor the apparent and the true ileal digestibility ofCP and AA, in growing pigs between 25 and 50 kg LW. Compared with the HA method, the peptide-alimentation method yielded higher estimates of ileal endogenous CP and AA acid losses in pigs given casein-based diets, although these two methods yielded similar estimates of true ileal AA and CP digestibility.

scholarly journals 183 Isoquinoline alkaloids increase apparent ileal digestibility of amino acids and crude protein and apparent total tract digestibility of crude protein by finishing pigs fed corn-soybean meal diets

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 81-82
Author(s):  
Carly M Rundle ◽  
Hans H Stein
Keyword(s):  
Amino Acids ◽  
Soybean Meal ◽  
Crude Protein ◽  
Latin Square ◽  
Sampling Technique ◽  
Finishing Pigs ◽  
Isoquinoline Alkaloids ◽  
Grab Sampling ◽  
Ileal Digestibility ◽  
Quadratic Effects

Abstract An experiment was conducted to determine if the inclusion of a preparation of isoquinoline alkaloids (IQ) in corn-soybean meal diets fed to finishing pigs increases the apparent ileal digestibility (AID) of amino acids (AA) and crude protein (CP) and the apparent total tract digestibility (ATTD) of CP and gross energy. Twelve ileal cannulated barrows (BW: 77.2 ± 6.23 kg) were allotted to a triplicated 4 x 3 incomplete Latin square design with 4 diets and 9 replicates per diet. Diets were supplemented with either 0, 40, 80, or 160 mg/kg IQ and with 0.40% titanium dioxide as an indigestible marker. Each collection period lasted 14 d with d 1 through 10 as an adaptation to the diets, fecal samples were taken using the grab sampling technique on d 11 and 12, and ileal digesta were collected for 8 h on d 13 and 14. Data were analyzed using the Proc MIXED procedure of SAS. Contrast statements were used to determine linear and quadratic effects of including IQ in the diets. Results were considered significant at P ≤ 0.05 and considered a trend at 0.05 &lt; P ≤ 0.10. Inclusion of IQ resulted in a quadratic increase (P &lt; 0.05) in the AID of CP and AA in corn-soybean meal diets fed to finishing pigs, with the greatest digestibility values generally observed in the 40 mg/kg IQ treatment. Additionally, IQ resulted in a quadratic increase (P = 0.009) in the ATTD of CP, with the greatest digestibility observed in the 80 mg/kg IQ treatment. Therefore, it was concluded that supplementing corn-soybean meal diets with 40 or 80 mg/kg IQ may increase the AID of CP and AA and ATTD of CP by finishing pigs.

Digestibility of amino acids in pigs fed distillers dried grains with solubles derived from corn, sorghum, and triticale

2020 ◽  
Author(s):  
Chan Sol Park ◽  
Darryl Ragland ◽  
O. Adeola
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Latin Square ◽  
Experimental Period ◽  
Growing Pigs ◽  
Initial Body Weight ◽  
Isoleucine Leucine ◽  
Ileal Digestibility ◽  
Distillers Dried Grains ◽  
Dietary Treatments

An experiment was conducted to determine the standardized ileal digestibility (SID) of amino acids (AA) in distillers dried grains with solubles derived from corn (C-DDGS), sorghum (S-DDGS), and triticale (T-DDGS) fed to growing pigs. A total of 16 barrows (initial body weight = 23.1 ± 2.03 kg) surgically fitted with T-cannulas at the distal ileum were divided into 4 groups based on body weight and assigned to a quadruplicated 4 × 2 incomplete Latin Square design with 4 dietary treatments and 2 experimental periods. Three diets containing C-DDGS, S-DDGS, or T-DDGS and a nitrogen-free diet were fed in each experimental period consisting of 5-d adaptation and 2-d ileal digesta collection. Pigs fed S-DDGS had less (P < 0.05) SID of histidine, isoleucine, leucine, and methionine compared with those fed T-DDGS, which were less (P < 0.05) than the values observed from pigs fed C-DDGS. The SID of arginine, phenylalanine, threonine, and valine in C-DDGS were greater (P < 0.05) than in S-DDGS but were not different from those in T-DDGS. The SID of lysine in C-DDGS was greater (P < 0.05) than in S-DDGS and T-DDGS. In conclusion, AA in S-DDGS were less digestible than those in C-DDGS and T-DDGS.

Amino acid digestibility in six sources of meat and bone meal, blood meal, and soybean meal fed to growing pigs

2018 ◽  
Vol 98 (4) ◽  
pp. 860-867 ◽  
Author(s):  
D.M.D.L. Navarro ◽  
J.K. Mathai ◽  
N.W. Jaworski ◽  
H.H. Stein
Keyword(s):  
Soybean Meal ◽  
Blood Meal ◽  
Crude Protein ◽  
Latin Square ◽  
Sole Source ◽  
Growing Pigs ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Initial Body Weight ◽  
Ileal Digestibility

Standardized ileal digestibility (SID) of amino acids (AAs) by growing pigs was determined in blood meal and six sources of meat and bone meal (MBM). Eighteen ileal-cannulated barrows (initial body weight: 69.3 ± 4.4 kg) were randomly allotted to a replicated 4 × 9 incomplete Latin square design with four periods and nine diets, giving eight replications per diet. One diet included 33% soybean meal (SBM) as the sole source of AA. Seven diets contained 9% blood meal or 9% of one of the six sources of MBM and 22% SBM as the only AA containing ingredients. The last diet was a nitrogen (N) free diet. Results indicated that the SID of all AAs were different (P < 0.05) among the six sources of MBM, but the SID of lysine (Lys) could not be predicted from the ratio between Lys and crude protein. For some, but not all AAs, the average SID in MBM was greater (P < 0.05) than in blood meal, but for most AAs the SID in MBM was less (P < 0.05) than in SBM. It is concluded that, as is the case for most other co-products, differences in concentration and SID of AAs among sources of MBM exist.

scholarly journals 172 Increasing inclusion of fermentable fiber decreases nutrient digestibility in grower pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 85-85
Author(s):  
Charlotte Heyer ◽  
L F Wang ◽  
R T Zijlstra
Keyword(s):  
Plasma Protein ◽  
Corn Starch ◽  
Latin Square ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Ileal Digestibility ◽  
Low Viscosity ◽  
Acacia Gum ◽  
Bovine Plasma ◽  
Total P

Abstract Fermentable fiber may increase endogenous losses of P and AA, thereby reducing apparent nutrient digestibility. Acacia gum fiber with medium-to-high fermentability and low viscosity was used to investigate its effect on apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients, and standardized total tract digestibility (STTD) of P in grower pigs. A P-free basal diet (49% corn starch; 18% bovine plasma protein) was formulated to measure basal endogenous P losses (EPL). Three diets were formulated to include 2.5, 5.0, or 7.5% acacia gum fiber at the expense of corn starch in the P-free basal diet. Diets contained 16.1–17.4% CP and 0.31–0.33% total P (DM-basis). The 4 diets were fed to 8 ileal-cannulated barrows (initial BW, 54.6 kg) for four 9-d periods in a double 4 × 4 Latin square. Apparent hindgut fermentation (AHF) was ATTD minus AID. Increasing inclusion of acacia gum quadratically decreased (P &lt; 0.01) AID of DM (∆ 11.1%), linearly decreased (P &lt; 0.05) ATTD of DM (∆ 1.7%) CP (∆ 1.2%), and quadratically increased (P &lt; 0.05) AHF of DM (∆ 9.4%). Basal EPL were 391 and 377 mg/kg DM intake (DMI) for ileum and total tract, respectively. Increasing inclusion of fiber linearly increased (P &lt; 0.05) ileal EPL (∆ 184 mg/kg DMI), and tended to linearly increase (P &lt; 0.10) EPL for total tract (∆ 243 mg/kg DMI). Dietary inclusion of acacia gum tended to linearly decrease (P &lt; 0.10) AID of P, but did not affect (P &gt; 0.10) ATTD, or STTD of P. In conclusion, increasing inclusion of fermentable, low viscous acacia gum fiber decreased diet digestibility of DM and ATTD of CP, but did not affect total tract P digestibility, indicating that increasing fermentable fiber did not increase specific endogenous losses of P in the total tract.

Ileal digestibility of protein and amino acids of feed peas with different trypsin inhibitor activity in pigs

2000 ◽  
Vol 80 (4) ◽  
pp. 643-652 ◽  
Author(s):  
F. Grosjean ◽  
C. Jondreville ◽  
I. Williatte-Hazouard ◽  
F. Skiba ◽  
B. Carrouée ◽  
...  
Keyword(s):  
Amino Acids ◽  
Trypsin Inhibitor ◽  
Crude Protein ◽  
Trypsin Inhibitor Activity ◽  
Rectal Anastomosis ◽  
Inhibitor Activity ◽  
Ileal Digestibility ◽  
Physical Chemical ◽  
Protein Amino Acids ◽  
Weight Proportion

Ileal digestibility of protein and amino acids was measured in pigs fed 13 round, tannin-free peas samples and related to the following physical, chemical and biological characteristics of these samples: thousand-seed weight, proportion of hulls, starch, fibre, crude protein, ether extract and ash contents, trypsin inhibitor activity and trypsin inhibitor activity per unit of crude protein (TIAP). Each pea sample was included in a diet containing starch, sucrose, minerals and vitamins and fed to four barrows (50 to 100 kg) fitted with an end-to-end ileo-rectal anastomosis. Standardised ileal protein and amino acid digestibilities, except for alanine of peas decreased linearly with increasing TIAP (P < 0.01) and was not affected by fiber content. For example standardized ileal digestibilities values (%) decreased by −0.1975, −0.1617, −0.2171, −0.2630, −0.2029 and −0.3536 per unit of TIAP (expressed in unit of trypsin inhibited per milligram crude protein), respectively, for crude protein and lysine, threonine, methionine, cystine and tryptophan. Key words: Peas, trypsin inhibitor activity, standardised ileal digestibilities, protein, amino acids, pig

PSVI-13 Amino Acid Supplementation During the Adaptation Period Did Not Affect the Standardized Ileal Digestibility of Amino Acids in Corn and Soybean Meal Fed to Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 200-201
Author(s):  
Hyunjun Choi ◽  
Sun Jong You ◽  
Beob Gyun G Kim
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Latin Square ◽  
Sole Source ◽  
Adaptation Period ◽  
Amino Acid Supplementation ◽  
Ileal Digestibility ◽  
Indispensable Amino Acids ◽  
Dietary Treatments

Abstract The objective was to determine the influence of amino acid (AA) supplementation during the adaptation period on the ileal digestibility of crude protein and AA in corn and soybean meal (SBM). Six barrows with an initial body weight of 30.9 ± 2.6 kg fitted with a T-cannula in the distal ileum were assigned to a 6 × 6 Latin square design with 6 dietary treatments and 6 periods. Two experimental diets contained corn or SBM as the sole source of AA and an N-free diet was additionally prepared. For AA supplementation groups, an AA mixture consisted of Gly, Lys, Met, Thr, Trp, Ile, Val, His, and Phe was added to the corn diet and the N-free diet at the expense of cornstarch, and an AA mixture of Lys, Met, and Thr was added to the SBM diet. All diets contained 0.5% of chromic oxide. The 6 experimental diets were fed to the pigs for 4 and half days, and the 3 diets containing AA mixture were switched to the respective diets without AA mixture during the following 2 and half days. Ileal digesta were collected during the last 2 days. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Arg and Trp in corn (P &lt; 0.05), but did not affect that in SBM. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Pro and Gly regardless of feed ingredient (P &lt; 0.05), but did not affect that of other AA. All AA except Pro in corn and SBM were unaffected by the addition of AA mixture during the adaptation period. In conclusion, the addition of amino acid during the adaptation period does not affect the standardized ileal digestibility of indispensable amino acids in feed ingredients.

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