scholarly journals 183 Isoquinoline alkaloids increase apparent ileal digestibility of amino acids and crude protein and apparent total tract digestibility of crude protein by finishing pigs fed corn-soybean meal diets

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 81-82
Author(s):  
Carly M Rundle ◽  
Hans H Stein

Abstract An experiment was conducted to determine if the inclusion of a preparation of isoquinoline alkaloids (IQ) in corn-soybean meal diets fed to finishing pigs increases the apparent ileal digestibility (AID) of amino acids (AA) and crude protein (CP) and the apparent total tract digestibility (ATTD) of CP and gross energy. Twelve ileal cannulated barrows (BW: 77.2 ± 6.23 kg) were allotted to a triplicated 4 x 3 incomplete Latin square design with 4 diets and 9 replicates per diet. Diets were supplemented with either 0, 40, 80, or 160 mg/kg IQ and with 0.40% titanium dioxide as an indigestible marker. Each collection period lasted 14 d with d 1 through 10 as an adaptation to the diets, fecal samples were taken using the grab sampling technique on d 11 and 12, and ileal digesta were collected for 8 h on d 13 and 14. Data were analyzed using the Proc MIXED procedure of SAS. Contrast statements were used to determine linear and quadratic effects of including IQ in the diets. Results were considered significant at P ≤ 0.05 and considered a trend at 0.05 < P ≤ 0.10. Inclusion of IQ resulted in a quadratic increase (P < 0.05) in the AID of CP and AA in corn-soybean meal diets fed to finishing pigs, with the greatest digestibility values generally observed in the 40 mg/kg IQ treatment. Additionally, IQ resulted in a quadratic increase (P = 0.009) in the ATTD of CP, with the greatest digestibility observed in the 80 mg/kg IQ treatment. Therefore, it was concluded that supplementing corn-soybean meal diets with 40 or 80 mg/kg IQ may increase the AID of CP and AA and ATTD of CP by finishing pigs.

2016 ◽  
Vol 56 (12) ◽  
pp. 2023
Author(s):  
Santi D. Upadhaya ◽  
Je-hoon Ryu ◽  
Kyung-il Kang ◽  
Seong-Jun Cho ◽  
In Ho Kim

Apparent ileal digestibility and standardised ileal digestibility of energy, dry matter, nitrogen and amino acids were evaluated in five cannulated [(Landrace × Yorkshire) × Duroc)] barrows with an initial bodyweight of 27.1 ± 0.58 kg in a 5 × 5 Latin square design. Pigs were fed unfermented soybean meal (USM) as control and fermented soybean meal A, B and C (FSMA, FSMB and FSMC, respectively), with 52.4%, 62.0% and 71.8% protein solubility, respectively, and a nitrogen-free diet. The nitrogen-free diet was used to determine basal endogenous losses of crude protein and amino acids. The apparent ileal digestibility of dry matter and nitrogen was greater (P < 0.05) in FMSC and tended to improve in FSMA and FSMB compared with the control. The apparent ileal digestibility of isoleucine, phenylalanine and valine was greater in all fermented soybean meal whereas that of lysine was greater (P < 0.05) in FSMC compared with USM. Likewise, the apparent ileal digestibility of aspartic acid and proline was greater (P < 0.05) in FSMA and FSMC. The standardised ileal digestibility of crude protein and lysine was higher (P < 0.05) in FSMC and tended to improve in FSMA and FSMB whereas the standardised ileal digestibility of isoleucine was higher (P < 0.05) in FSMA, FSMB and FSMC compared with USM. In conclusion, fermentation of soybean meal showed better digestibility compared with USM regardless of its protein solubility values.


1996 ◽  
Vol 76 (4) ◽  
pp. 563-569 ◽  
Author(s):  
M. Z. Fan ◽  
W. C. Sauer ◽  
V. M. Gabert

Studies were conducted with six barrows, average initial body weigh 32.5 kg, fitted with a T-cannula at the distal ileum, to determine some of the factors responsible for the variability in apparent ileal digestibility values of amino acids (AA) in canola meal. The barrows were fed six corn starch-based diets, formulated to contain 18.0% crude protein (CP) on a dry matter (DM) basis from six canola meal samples, according to a 6 × 6 Latin square design. Chromic oxide (0.3%) was used as a digestibility marker. The pigs were fed twice daily, at 08:00 and 20:00 h. The dietary allowance was 1600 g d−1. Each experimental period consisted of 8 d. Ileal digesta were collected at 2-h intervals, for a total of 24 h from 08:00 on day 6 to 08:00 h on day 8. With the exception of proline, there were differences (P < 0.05) in the apparent ileal digestibility values of all AA among the six canola meal samples. Of the indispensable AA in canola meal, the digestibility values of arginine, histidine, and methionine were relatively high, ranging from 79.4 to 84.4, from 76.5 to 81.0%, and from 77.3 to 82.4%, respectively, while the digestibility values of threonine and tryptophan were relatively low, ranging from 59.7 to 66.5 and from 61.7 to 67.5%, respectively. Furthermore, with the exception of arginine, the digestibility values of the indispensable AA were negatively correlated (P < 0.05) with the neutral-detergent fiber (NDF) content in canola meal. In addition with the exception of threonine, tryptophan and valine, the digestibility values of the indispensable AA were positively correlated (P < 0.05) with their respective dietary levels. In conclusion, measured with the ileal analysis method, there were differences (P < 0.05) in the digestibility values of the majority of AA among the canola meal samples. Differences in NDF content were mainly responsible for the variation. In addition, differences in the dietary levels of most AA also contributed, in part, to the variation of AA digestibility values. Key words: Amino acids, ileal digestibility, canola meal, pigs


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 175-175
Author(s):  
Élisabeth Chassé ◽  
Frédéric Guay ◽  
Marie-Pierre Létourneau-Montminy

Abstract This study was conducted to determine the effect of pelleting on the digestibility of corn-soybean meal-based diet in growing pigs. Two trials with 6 pigs cannulated at the distal ileum were conducted. In each trial, pigs were assigned to each treatment following a crossover design. In each experiment, the same diet, composed of corn and soybean meal with 10% wheat from two different feed mills, was served in pellet or mash form. Pelleting allowed an increase in digestibility in one of the trials. Apparent ileal digestibility (AID) of dry matter (DM), crude protein (CP) and digestible energy (DE) were improved with pelleting by 8, 12 and 9% (P&lt; 0.01). The AID of amino acids (AA) was also improved (P&lt; 0.05). Apparent total tract digestibility (ATTD) was increased by pelleting in DM, CP and DE by 5, 7 and 6% respectively (P&lt; 0.01). The digestibility of the mash diet in experiment 1 was lower than in the pelleted diet in the experiment 1 and both diets in experiment 2 as shown by the interaction Pelleting X Trial which was significant for the AID and ATTD of DM, CP and DE (P&lt; 0.01). Therefore, in experiment 1, pelleting allows to improve the digestibility of diet to the same level as in experiment 2. The AID of CP was higher by 37% in the mash diet from the second experiment compared to the one in the first experiment. Even though the same ingredients were chosen in the two experiments, this shows the variability in digestibility existing between different feed mills and ingredient sources. This difference was not observed in pelleted diets. The results obtained in these two trials show that pelleting can reduce the variability of digestibility and then give a good digestibility of diets even if the ingredients are of different quality or sources.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 200-201
Author(s):  
Hyunjun Choi ◽  
Sun Jong You ◽  
Beob Gyun G Kim

Abstract The objective was to determine the influence of amino acid (AA) supplementation during the adaptation period on the ileal digestibility of crude protein and AA in corn and soybean meal (SBM). Six barrows with an initial body weight of 30.9 ± 2.6 kg fitted with a T-cannula in the distal ileum were assigned to a 6 × 6 Latin square design with 6 dietary treatments and 6 periods. Two experimental diets contained corn or SBM as the sole source of AA and an N-free diet was additionally prepared. For AA supplementation groups, an AA mixture consisted of Gly, Lys, Met, Thr, Trp, Ile, Val, His, and Phe was added to the corn diet and the N-free diet at the expense of cornstarch, and an AA mixture of Lys, Met, and Thr was added to the SBM diet. All diets contained 0.5% of chromic oxide. The 6 experimental diets were fed to the pigs for 4 and half days, and the 3 diets containing AA mixture were switched to the respective diets without AA mixture during the following 2 and half days. Ileal digesta were collected during the last 2 days. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Arg and Trp in corn (P &lt; 0.05), but did not affect that in SBM. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Pro and Gly regardless of feed ingredient (P &lt; 0.05), but did not affect that of other AA. All AA except Pro in corn and SBM were unaffected by the addition of AA mixture during the adaptation period. In conclusion, the addition of amino acid during the adaptation period does not affect the standardized ileal digestibility of indispensable amino acids in feed ingredients.


1999 ◽  
Vol 79 (4) ◽  
pp. 467-475 ◽  
Author(s):  
M. Z. Fan ◽  
W. C. Sauer

Six barrows, average initial body weight 32.5 kg, fitted with a simple T-can-nula at the distal ileum, were used to investigate factors responsible for the variability of apparent ileal amino acid (AA) digestibility values among six pea samples. The barrows were fed six corn-starch-based diets, formulated to contain 16.5% crude protein (CP) (as-fed) from six different pea samples, according to a 6 × 6 Latin square design. Chromic oxide (0.3%) was used as the digestibility marker. The pigs were fed twice daily, at 0800 and 2000 h. The dietary allowance was 1600 g−1. Each experimental period lasted 7 d. Ileal digesta were collected at 2-h intervals for a total of 24 h from 0800 h on day 6 to 0800 h on day 8. With the exception of arginine, cysteine, histidine, and methionine, there were differences (P < 0.05) in the apparent ileal digestibility values of the indispensable including semi-essential (+semi-) AA among the pea samples. Of the indispensable AA (+semi-) within each pea sample, the digestibility values of arginine and lysine were relatively high, ranging from 88.3 to 91.3% and from 78.7 to 85.2%, respectively, while the digestibility values of cysteine, methionine, and tryptophan were relatively low, ranging from 53.8 to 62.7%, from 69.4 to 75.4%, and from 53.1 to 70.4%, respectively. With the exception of arginine, cysteine, and tryptophan, the ileal digestibility values of the indispensable AA (+semi-) were negatively correlated (P < 0.05) with the neu-tral-detergent fiber content in the pea samples. Of all the AA, only the digestibility of tryptophan was negatively correlated (P < 0.05) with the trypsin inhibitor activity in the pea samples. The significant finding of this study was that with the exception of arginine, cysteine, and proline, differences in the digestibility values of all other AA in the pea samples were positively correlated (P < 0.05) with their respective dietary AA contents. This suggests that differences in AA levels in the assay diets were responsible for the variability of apparent ileal digestibility values of AA between pea samples. Therefore, to eliminate this methodological effect, true rather than apparent ileal AA digestibility values for peas should be determined. Key words: Amino acids, ileal digestibility, peas, pigs


1978 ◽  
Vol 58 (1) ◽  
pp. 87-96 ◽  
Author(s):  
D. M. ANDERSON ◽  
J. M. BELL ◽  
G. I. CHRISTISON

Oats (Avena sativa L. cv. Hinoats, 14.6% crude protein, 4.66 Mcal GE/kg) were tested in digestion and feeding trials with growing–finishing pigs from 23 to 91 kg body weight. Up to 57 kg, groups of six crossbred pigs (3 male, 3 female) in a 3 × 3 factorial experiment were fed supplemented diets of Hinoats meal, Hinoats pellets or 2/3 Hinoats:1/3 wheat, each with one of three supplements: either 0.2% lysine plus 0.05% methionine, or with half levels of these amino acids plus 2% soybean meal, or 6% soybean meal. At 57 kg, the pigs were assigned from each grower ration to one of three finisher rations, these being the three grower rations supplemented only with amino acids. Feeding was allowed for three 1-h periods daily up to 57 kg body weight and for two 1-h periods thereafter. Pigs of 23–57 kg fed pellets had greater daily feed intakes than pigs fed meal or the meal ration containing wheat (1.90, 1.70, 1.77 kg, respectively) and grew faster (0.69, 0.60, 0.60 kg/day, respectively). From 57 kg body weight, performance was not affected by previous diet but pigs fed pellets ate more and grew faster than the others. Hinoats rations were efficiently utilized by the pigs. In separate digestion trials, Hinoats energy and protein were found to be 72 and 82% digestible, respectively.


Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1434 ◽  
Author(s):  
Jia-Cheng Yang ◽  
Li Wang ◽  
Ya-Kuan Huang ◽  
Lei Zhang ◽  
Rui Ma ◽  
...  

The current study evaluated the influence of a multi-carbohydrase and phytase complex (MCPC) on the ileal and total tract digestibility of nutrients in growing pigs. A total of eight barrows (initial BW = 30.7 ± 1.1 kg) were surgically fitted with a T-cannula at the distal ileum and randomly allotted to four groups. The experiment was conducted according to a 4 × 4 Latin square design, each period lasting 10 days. Pigs were fed four experimental diets, which consisted of two basal diets (BD1, low phytate; BD2, high phytate) with or without MCPC containing at least 1800 U xylanase, 6600 U α-arabinofuranosidase, 1244 U β-glucanase, and 1000 U phytase per/kg corn–soybean meal with 15% corn distillers based diet. The high phytate diet reduced (p < 0.05) the apparent ileal digestibility (AID) of crude protein by 1.4% and the apparent total tract digestibility (ATTD) of organic matter, crude protein, and gross energy by 1.7, 2.3, and 1.9%, respectively, and tended to decrease (p = 0.10) the ATTD of Ca by 17.3%, relative to the low phytate diet. The dietary supplementation of the MCPC increased (p < 0.05) the AID of phosphorus (P) and calcium (Ca) by 34.2% and 31.1% for BD1 and 26.7% and 41.3% for BD2, respectively, and increased (p < 0.05) ATTD of crude fat, P, and Ca by 1.4%, 45.6%, and 9.6% for BD1 and 3.1%, 66.0%, and 52.7% for BD2, respectively. The MCPC supplementation did not significantly increase the AID and (or) ATTD of crude protein, organic matter, and starch. In conclusion, the dietary supplementation of the MCPC could improve the AID of P and Ca and the ATTD of crude fat, P, and Ca.


Author(s):  
Jimena A Ibagon ◽  
Su A Lee ◽  
Hans H Stein

Abstract The objective was to test the hypothesis that there is no effect of origin or processing procedure on the standardized ileal digestibility (SID) of amino acids (AA) and crude protein (CP) in sunflower co-products. Six sources of sunflower meal (SFM) and 1 source of sunflower expellers (SFE) were obtained from Ukraine, Italy, Hungary, and the U.S. Each source of SFM or SFE was the only source of CP and AA in 1 diet and an N-free diet was also used for a total of 8 diets. Eight barrows (body weight: 28.5 ± 2.4 kg) had a T-cannula installed in the distal ileum and were allotted to 1 of the 8 diets using an 8 × 8 Latin square design with 8 periods. The Lys:CP ratio in the 6 sources of SFM ranged from 3.10 to 3.96% with CP concentrations ranging from 27.34 to 36.75%. Crude protein in SFE was 26.87% and the Lys:CP ratio was 3.51%. Concentrations of acid hydrolyzed ether extract in the 6 sources of SFM ranged from 0.60 to 3.11%, but SFE contained 8.77%. Results indicated that the SID of CP was lower (P &lt; 0.05) in SFM from Hungary compared with all other sources of SFM except for 1 of the sources from Ukraine. There were no differences in the SID of Lys, Met, and Trp among sources of SFM, but for most of the remaining indispensable AA, the SFM from Hungary had less (P &lt; 0.05) SID than the other sources. However, only few differences in SID of indispensable AA were observed among the other sources of SFM, but the SID of CP and all AA except Trp was greater (P &lt; 0.05) in SFE compared with SFM. In conclusion, there were only few differences in the SID of the first limiting AA among SFM obtained from Ukraine, Hungary, Italy, and the U.S., but the SID of CP and AA was greater in SFE than in SFM indicating that processing of sunflower seeds influence the nutritional value.


2020 ◽  
Vol 98 (8) ◽  
Author(s):  
Maryane S F Oliveira ◽  
John K Htoo ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that values for standardized ileal digestibility (SID) of amino acids (AA) in corn, wheat, and wheat middlings obtained using the direct procedure are not different from values obtained using the difference procedure. Sixteen ileal-cannulated barrows (initial BW: 69.5 ± 5.0 kg) were allotted to a replicated 8 × 4 Youden Square design with 8 diets and 4 periods. Each period consisted of 5 d of adaptation to the diet and 2 d of collection of ileal digesta. Four diets were based on soybean meal (SBM), corn, wheat, or wheat middlings as the only AA-containing ingredients. Three additional diets were based on a mixture of SBM and corn, SBM and wheat, or SBM and wheat middlings, and an N-free diet was also used. The apparent ileal digestibility (AID) and the SID of crude protein (CP) and AA for the 4 diets containing SBM, corn, wheat, or wheat middlings as the sole source of AA were calculated using the direct procedure. The AID and SID of CP and AA for the 3 mixed diets containing SBM and corn, wheat, or wheat middlings were also calculated and the contribution of digestible AA from SBM was subtracted from the AID or SID values for the diets. The AID or SID of AA in corn, wheat, and wheat middlings were subsequently calculated by difference. Results indicated that the AID values for a few AA were lower (P &lt; 0.05) if the direct procedure was used instead of the difference procedure, regardless of ingredient. The AID of Trp was greater in corn and wheat middlings, and the SID of Trp in corn and wheat middlings tended to be greater, if the direct procedure rather than the difference procedure was used, but that was not the case for wheat (interaction, P &lt; 0.05 and P &lt; 0.10, respectively). However, for all other indispensable AA, and for most of the dispensable AA, the SID of AA in corn, wheat, and wheat middlings was not different between the difference procedure and the direct procedure. Therefore, values for SID of AA in cereal grains and fiber-rich ingredients may be determined using either the direct or the difference procedure.


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