Involvement of abscisic acid metabolites and the oxidative status of barley genotypes in response to drought

2014 ◽  
Vol 94 (8) ◽  
pp. 1481-1490 ◽  
Author(s):  
A. Thameur ◽  
A. Ferchichi ◽  
M. López-Carbonell
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Oxidative Status ◽  
Antioxidant Compounds ◽  
Phaseic Acid ◽  
Hordeum Vulgare L ◽  
Drought Treatment ◽  
Acid Metabolites ◽  
Glucosyl Ester ◽  
Barley Genotypes

Thameur, A., Ferchichi, A. and López-Carbonell, M. 2014. Involvement of abscisic acid metabolites and the oxidative status of barley genotypes in response to drought. Can. J. Plant Sci. 94: 1481–1490. Endogenous concentrations of free abscisic acid (ABA), abscisic acid glucosyl ester (ABAGE), phaseic acid (PA), dihydrophaseic acid (DPA) and 7′-hydroxy ABA (7′-OH ABA) were analysed by means of a LC–MS/MS system in five genotypes of barley (Hordeum vulgare L.) grown under well-watered and drought stress conditions. For this purpose a drought treatment was conducted using genotypes: ‘Ardahoui’, ‘Manel’, ‘Pakistan’, ‘Rihane’ and ‘Roho’. Our results show that free and conjugated ABA levels increased in all genotypes grown under water stress, except in Manel genotype, in which ABAGE levels were the lowest. In contrast, genotypes Ardhaoui and Roho showed the highest ABA and ABAGE levels. Nevertheless, drought Rihane plants showed the maximum ability to increase the endogenous ABA concentrations. PA, DPA and 7′-OH ABA increased also in all drought genotypes, especially in the leaves of Manel and Roho genotypes, while the highest ability to increase the endogenous PA content corresponded to genotypes Ardahoui and Pakistan. To evaluate the plant oxidative status, some antioxidant compounds were analysed. Under drought conditions, small changes in some of them were seen. Among the genotypes studied, Manel was the only one which did not show increases in malondialdehyde (MDA) and, in parallel, showed a decrease in ABAGE content. These results provide us valuable information and contribute to the knowledge of the different responses of these genotypes to drought stress.

The effect of abscisic acid and abscisic acid metabolites on the germination of cress seed

1992 ◽  
Vol 70 (8) ◽  
pp. 1550-1555 ◽  
Author(s):  
L. V. Gusta ◽  
B. Ewan ◽  
M. J. T. Reaney ◽  
S. R. Abrams
Keyword(s):  
Abscisic Acid ◽  
Seed Germination ◽  
Optical Isomers ◽  
Phaseic Acid ◽  
Naturally Occurring ◽  
Maximum Inhibition ◽  
Cress Seed ◽  
Acid Metabolites ◽  
Aba Analogs ◽  
Optically Pure

Optical isomers of abscisic acid (ABA) and racemic mixtures of both abscisic acid and abscisic acid metabolites were studied to determine their effects on the emergence of root primordia and cotyledons from cress seed. The relative emergence sensitivity of cress seed to the racemic compounds was (±)-ABA aldehyde ≥ (±)-ABA alcohol > (±)-ABA > (±)7′-hydroxy ABA > (±)-phaseic acid. Thus ABA and ABA precursors were effective inhibitors whereas the ABA catabolites, phaseic acid, and 7′-hydroxy ABA had little or no effect on germination. The naturally occurring optically pure enantiomer (+)ABA was a more potent germination inhibitor than synthetic (−)-ABA. An ABA analog, 2′,3′-cis dihydro ABA (DHABA), that is not metabolized to phaseic acid was also studied for inhibitory activity. Although optically pure DHABA has the same configuration at C-1 as (+)-ABA, it was less inhibitory than (+)-ABA and its (−) enantiomer was inactive. The pattern of activity observed in treatments with the enantiomers of DHABA indicates that the configuration at C-1′ is important for maximum inhibition of cress seed germination. It also suggests that in contrast to monocot seeds, the formation of phaseic acid is not required for the inhibition of cress seed germination. Key words: abscisic acid, phaseic acid, ABA alcohol, ABA aldehyde, 7′OHABA, germination, ABA analogs.

scholarly journals Exogenous Application of Proline and Salicylic Acid can Mitigate the Injurious Impacts of Drought Stress on Barley Plants Associated with Physiological and Histological Characters

2020 ◽  
Vol 12 (5) ◽  
pp. 1736 ◽  
Author(s):  
Khaled A. A. Abdelaal ◽  
Kotb A. Attia ◽  
Salman F. Alamery ◽  
Mohamed M. El-Afry ◽  
Abdelhalim I. Ghazy ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Antioxidant Enzymes ◽  
Drought Stress ◽  
Grain Yield ◽  
Chlorophyll Concentration ◽  
Stem Length ◽  
Hordeum Vulgare L ◽  
Drought Treatment ◽  
Growing Seasons ◽  
Relative Water

Barley is a very important crop worldwide and has good impact in preserving food security. The impacts of 10 mM proline and 0.5 mM salicylic acid were evaluated on water stressed barley plants (Hordeum vulgare L. Giza126). Salicylic acid and proline treatments led to increased stem length, plant dry weights, chlorophyll concentration, relative water content, activity of antioxidant enzymes, and grain yield under drought stress. Nevertheless, lipid peroxidation, electrolyte leakage (EL), superoxide (O2·−), and hydrogen peroxide (H2O2) significantly decreased in treated barley plants with proline and salicylic acid in both growing seasons as compared with drought treatment only, which caused significant decrease in stem length, plant dry weights, chlorophyll concentration, activity of antioxidant enzymes, as well as biological and grain yield. These results demonstrated the importance of salicylic acid and proline as tolerance inducers of drought stress in barley plants.

scholarly journals Flavonoids improve drought tolerance of maize seedlings by regulating the homeostasis of reactive oxygen species

2021 ◽  
Author(s):  
Baozhu Li ◽  
Ruonan Fan ◽  
Guiling Sun ◽  
Ting Sun ◽  
Yanting Fan ◽  
...  
Keyword(s):  
Free Radicals ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Infrared Imaging ◽  
Oxygen Free Radicals ◽  
Transcriptome Profiling ◽  
Far Infrared ◽  
Drought Treatment ◽  
Physiological Characterization ◽  
Oxidative Damages

Abstract Background and aims As drought threatens the yield and quality of maize (Zea mays L.), it is important to dissect the molecular basis of maize drought tolerance. Flavonoids, participate in the scavenging of oxygen free radicals and alleviate stress-induced oxidative damages. This study aims to dissect the function of flavonoids in the improvement of maize drought tolerance. Methods Using far-infrared imaging screening, we previously isolated a drought overly insensitivity (doi) mutant from an ethyl methanesulfonate (EMS)-mutagenized maize library and designated it as doi57. In this study, we performed a physiological characterization and transcriptome profiling of doi57 in comparison to corresponding wild-type B73 under drought stress. Results Under drought stress, doi57 seedlings displayed lower leaf-surface temperature (LST), faster water loss, and better performance in growth than B73. Transcriptome analysis reveals that key genes involved in flavonoid biosynthesis are enriched among differentially expressed genes in doi57. In line with these results, more flavonols and less hydrogen peroxide (H2O2) were accumulated in guard cells of doi57 than in those of B73 with the decrease of soil water content (SWC). Moreover, the capacity determined from doi57 seedling extracts to scavenge oxygen free radicals was more effective than that of B73 under the drought treatment. Additionally, doi57 seedlings had higher photosynthetic rates, stomatal conductance, transpiration rates, and water use efficiency than B73 exposed to drought stress, resulting in high biomass and greater root/shoot ratios in doi57 mutant plants. Conclusion Flavonoids may facilitate maize seedling drought tolerance by lowering drought-induced oxidative damage as well regulating stomatal movement.

Stress-responsive tomato gene SlGRAS4 function in drought stress and abscisic acid signaling

Plant Science ◽  
2021 ◽  
Vol 304 ◽  
pp. 110804
Author(s):  
Yudong Liu ◽  
Ling Wen ◽  
Yuan Shi ◽  
Deding Su ◽  
Wang Lu ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Abscisic Acid Signaling ◽  
Tomato Gene

scholarly journals Comparing transcriptional responses to Fusarium crown rot in wheat and barley identified an important relationship between disease resistance and drought tolerance

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Z. Y. Su ◽  
J. J. Powell ◽  
S. Gao ◽  
M. Zhou ◽  
C. Liu
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Crown Rot ◽  
Differentially Expressed ◽  
Transcriptional Responses ◽  
Drought Treatment ◽  
Crop Species ◽  
Fusarium Crown Rot ◽  
Important Relationship ◽  
The Impact

Abstract Background Fusarium crown rot (FCR) is a chronic disease in cereal production worldwide. The impact of this disease is highly environmentally dependant and significant yield losses occur mainly in drought-affected crops. Results In the study reported here, we evaluated possible relationships between genes conferring FCR resistance and drought tolerance using two approaches. The first approach studied FCR induced differentially expressed genes (DEGs) targeting two barley and one wheat loci against a panel of genes curated from the literature based on known functions in drought tolerance. Of the 149 curated genes, 61.0% were responsive to FCR infection across the three loci. The second approach was a comparison of the global DEGs induced by FCR infection with the global transcriptomic responses under drought in wheat. This analysis found that approximately 48.0% of the DEGs detected one week following drought treatment and 74.4% of the DEGs detected three weeks following drought treatment were also differentially expressed between the susceptible and resistant isolines under FCR infection at one or more timepoints. As for the results from the first approach, the vast majority of common DEGs were downregulated under drought and expressed more highly in the resistant isoline than the sensitive isoline under FCR infection. Conclusions Results from this study suggest that the resistant isoline in wheat was experiencing less drought stress, which could contribute to the stronger defence response than the sensitive isoline. However, most of the genes induced by drought stress in barley were more highly expressed in the susceptible isolines than the resistant isolines under infection, indicating that genes conferring drought tolerance and FCR resistance may interact differently between these two crop species. Nevertheless, the strong relationship between FCR resistance and drought responsiveness provides further evidence indicating the possibility to enhance FCR resistance by manipulating genes conferring drought tolerance.

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