Natural heterogeneity of soil properties was used to explore their influence
on nitrogen (N) mineralisation and nitrification in undisturbed small soil
volumes (soil cells; c. 1 · 7
cm3 ) sampled from a small field plot (2 m by 3 m). Soil
cells (840) were randomly ascribed to 1 of 6 treatments in which soils were
retained continuously moist (M10 and
M30 treatments) and amended with organic N from clover
(Cl10 and Cl30 treatments), dried
and rewetted (DW10), or treated with urea
(Ur10) (subscripts indicate soil incubation at matric
potential - 10 or - 30 kPa). After 20 days of incubation at 24C, each soil
cell was analysed for NO-3 -N, NH
+ 4 -N, pH, bulk density (BD), volumetric water content (θv), water
content at - 490 kPa (θv490), and pH buffer capacity (pHBC). On 25 soil
cells from each treatment, % clay, % silt, % sand, total
N (% N), organic carbon (% C), and 7 cations and anions were
also determined.
Net N mineralisation and net nitrification occurred in all treatments, and the
total mineral N at the end of the incubation was 497, 81, 73, 31, 27, and 31
µg N/g in the Ur10 Cl10,
Cl30, M10,
M30, and DW10 treatments,
respectively. Net N mineralisation in the M30 treatment
was 84% of that in the M10 treatment, and net N
mineralisation in the Cl30 treatment was 86% of
that in the Cl10 treatment. Fluctuations in soil pH
varied markedly between treatments and over time, and it was apparent that
alkaline processes were occurring in all soil cells. The heterogeneity between
soil samples was substantial for all of the soil variables.
Soil variables were classified in a hierarchy from the least to the most
fundamental based on their stability through time. This ranking provides a
conceptual tool for understanding interrelationships between soil properties
and for interpreting results of regression analyses. The sampling approach
adopted in this study was designed to harness the natural heterogeneity of
soil properties in the small field site while keeping other properties and
environmental factors, that usually vary over larger distances, constant. Both
the extent of heterogeneity of soil properties and the nature of their
correlations with NO-3 -N
suggested that this technique would be useful in the exploration of how soil
properties influence N mineralisation and nitrification.