Urban soil microbial community and microbial-related carbon storage are severely limited by sealing

Purpose Urbanisation causes changes in land use, from natural or rural to urban, leading to the sealing of soil and the replacement of vegetation by buildings, roads and pavements. The sealing process impacts soil properties and services and can lead to negative consequences for microbial attributes and processes in soil. At present, information about the microbial community following soil sealing is limited. As such, we investigated how changes in soil physical and chemical properties caused by sealing affect the soil microbial community and soil ecosystem services. Material and methods Soils were sampled beneath impervious pavements (sealed) and from adjacent pervious greenspace areas (unsealed). Soil properties (total C, total N, C:N ratio and water content) and microbial attributes (microbial biomass C, N-mineralisation and phospholipid fatty acids—PLFA) were measured and correlated. Results and discussion A reduction of total C, total N, and water content were observed in sealed soil, whilst the C:N ratio increased. Sealed soil also presented a reduction in microbial attributes, with low N-mineralisation revealing suppressed microbial activity. PLFA data presented positive correlations with total C, total N and water content, suggesting that the microbial community may be reduced in sealed soil as a response to soil properties. Furthermore, fungal:bacterial and gram-positive:gram-negative bacterial ratios were lower in sealed soil indicating degradation in C sequestration and a consequential effect on C storage. Conclusions Sealing causes notable changes in soil properties leading to subsequent impacts upon the microbial community and the reduction of microbial activity and soil C storage potential.

Solid fraction of separated digestate as soil improver: implications for soil fertility and carbon sequestration

Purpose This study investigated the C and N mineralisation potential of solid fractions (SFs) from co-digestated pig manure after P-stripping (P-POOR SF) in comparison with P-rich SFs, as a means to estimate their organic matter stability in soil. Compost (COMP) and biochar (BCHR) (made from P-POOR SF) were also included in the study as reference biosolids. Methods The SFs were incubated in a sandy-loam soil under moist conditions to determine production of CO2 and mineral N. At specified intervals, CO2 evolution in the mixtures was measured via the alkali trap method and titration over a period of 81 days, while mineral N was measured using a flow analyser after KCl extraction over a period of 112 days. Results The various SFs showed similar patterns of C mineralisation (15–26% of added total C in 81 days) that were clearly higher than for COMP and BCHR (6% and 7%, respectively). Temporary N immobilisation was observed in biosolids with a high C/N ratio. The effective organic matter (EOM) of the SFs was calculated based on the C mineralisation data and varied between 130 and 369 kg Mg−1. Conclusions The SF with a reduced P content had a high EOM/P ratio which is beneficial in areas where P status of the soil is already high. Moreover, the N mineralisation patterns confirm that a high C/N ratio may also reduce risks for N leaching due to temporary N immobilisation.

The interactive effects of soil disturbance and residue quality on soil nitrogen mineralisation in a tropical sandy soil

Soil conservation practices, such as reduced and no tillage, have been found to enhance soil nitrogen (N) sequestration through decreasing the rate of N mineralisation of added organic materials. Nitrogen mineralisation is not only affected by tillage, but also by the quality (chemical composition) of the organic residues. This study evaluated the interaction of residue quality and soil disturbance on N mineralisation in a sandy soil. A 112-day incubation experiment was conducted with two levels of soil disturbance (undisturbed and disturbed conditions) and five plant residue amendments of contrasting quality. The contrasting quality (N, lignin (L), and polyphenols (Pp)) (in g kg–1) amendments follow: (i) unamended; (ii) Sesbania grandiflora (N 44, L 173, Pp 9.2); (iii) Indigofera hirsuta (N 41, L 177, Pp 30); (iv) Dipterocarpus tuberculatus (N 8.2, L 203, Pp 71); and (v) Eucalyptus camaldulensis (N 9.7, L 126, Pp 110). Residues (ii) and (iii) were fresh legume leaves, while (iv) and (v) were non-legume leaf litter. Disturbance only significantly increased N mineralisation rates in the legume-residue treated soils (increases of 18.8% for S. grandiflora and 27.1% for I. hirsuta) during the early stage of decomposition (first 14 days). In the legume treatment, disturbance significantly increased the ammonification, but decreased nitrification in soil relative to undisturbed soils. The difference in patterns of ammonification and nitrification was more pronounced in the early than in the later period of decomposition. This indicated an inhibitory effect of soil disturbance on nitrification, which was particularly pronounced in the legume-treated soils. The Pp content of residues was the major quality parameter regulating the soil ammonium-N and nitrate-N concentrations. Minimum soil disturbance should be adopted under legume soil organic amendment so that both ammonification and nitrification components of N mineralisation process can occur normally, and nitrate-loving crops can take up N in the form of nitrate-N which will enhance their yields. Moreover, undisturbed conditions under legume organic amendments reduced N mineralisation, resulting in enhancing soil N sequestration.

Predicting nitrogen mineralisation in Australian irrigated cotton cropping systems

Cotton cropping systems in Australia have poor nitrogen (N) use efficiency, largely due to over-application of N fertiliser. The N mineralisation from soil organic N reserves is often overlooked, or underestimated despite recent studies indicating that it may contribute the majority of N exported with the crop. Predicting N mineralisation is a major challenge for agricultural industries worldwide, as direct measurements are time-consuming and expensive, but there is considerable debate as to the most reliable methods for indirect estimation. Additionally, laboratory incubations assess potential (rather than actual) mineralisation, and may not be representative of N cycling rates in the field. We collected 177 samples from most major Australian cotton growing regions, and assessed their mineralisation potential using ex situ laboratory incubations, along with an assessment of potential indicators routinely measured in soil nutrient tests. Additionally, at three unfertilised sites we conducted in situ assessment of mineralisation by quantifying soil N at the beginning of the growing season, and soil and crop N at the end of the season. We found that Australian cotton cropping soils had substantial mineralisation potential, and that soil total N and total carbon were correlated with mineralisation, and have potential to be used for prediction. Other potential indicators such as carbon dioxide production and ammonium and nitrate concentrations were not correlated with mineralisation. In parallel studies of ex situ and in situ mineralisation, we found ex situ laboratory incubations overestimated mineralisation by 1.7 times on average. We discuss findings in terms of management implications for Australian cotton farming systems.

Effects of pH and mineralisation on nitrification in a subtropical acid forest soil

Effects of pH on nitrification have been explored widely; however, few researchers have investigated the compound effects of pH and mineralisation on nitrification and responsible nitrifiers in subtropical forest soils. An acid subtropical forest soil was subjected to pH treatments by liming for 40 days at 25°C and 60% water holding capacity. After 40 days, gross N transformation rates in the samples were evaluated using the 15N tracing technique and model. Ammonia-oxidising bacteria (AOB) and ammonia-oxidising archaea (AOA) were quantified by quantitative polymerase chain reaction. The results showed that gross rates of mineralisation (P < 0.01), NH4+ oxidation to NO3− (P < 0.01), and dissimilatory NO3− reduction to ammonium (DNRA, P < 0.05) significantly increased with elevated soil pH. The NH4+ oxidation to NO3− rates and N mineralisation rates were significantly correlated (P < 0.05). The abundance of AOB (P < 0.05) but not AOA amoA genes dramatically increased in the elevated pH treatments after 40 days. We suggest that the N mineralisation was sensitive to elevated pH in the subtropical acid forest soil, which increased the supply rate of nitrification substrate and the favourable pH microsites for nitrifiers. Interactions between elevated pH and mineralisation have a synergistic and stimulatory effect on development of AOB and their associated nitrifying activity.