Cell-Specific Growth Inhibition of Human Cervical Cancer Cell by Recombinant Adenovirus p53 in vitro and in vivo

To evaluate anti-tumor effects of recombinant adenovirus p53, time-course p53, E6 expression, and cell growth inhibition were investigated in vitro and in vivo using cervical cancer cell lines such as CaSki, SiHa, HeLa, HeLaS3, C33A, and HT3. The cell growth inhibition was studied via cell count assay, MTT assay and neutral red assay. After transfecting AdCMVp53 into SiHa cells-xenografted nude mice, the transduction efficiency and anti-tumor effect were investigated for a month. The results showed that adenoviral p53 expression induced significant growth suppression on the cancer cells, in which E6 transcript was strongly repressed, and that the expression of p53 and E6 were remarkably dependent on each cell type. The transduction efficiency was highly maintained in vivo as well as in vitro, and the size of tumor was remarkably decreased in comparison with AdCMVLacZ control. The results suggest that the adenovirus-mediated p53 gene transfection was done very effectively in vitro and in vivo experiment, and the cell growth was suppressed via p53-dependent apoptotic cell death, and that the anti-tumor effect could be related to E6 and p53 expression pattern.

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Hydralazine inhibits human cervical cancer cell growth in vitro in association with APC demethylation and re-expression

Cancer Chemotherapy and Pharmacology ◽

10.1007/s00280-008-0773-z ◽

2008 ◽

Vol 63 (4) ◽

pp. 605-613 ◽

Cited By ~ 42

Author(s):

Yinhong Song ◽

Changju Zhang

Keyword(s):

Cervical Cancer ◽

Cell Growth ◽

Cancer Cell ◽

Cervical Cancer Cell ◽

Cancer Cell Growth ◽

Human Cervical Cancer Cell ◽

Human Cervical Cancer

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Cytotoxic Effect of Syzgium aromaticum Extract and Gemcitabine on Human Cervical Cancer Cell Line

The Open Nutraceuticals Journal ◽

10.2174/18763960010030100069 ◽

2010 ◽

Vol 3 (1) ◽

pp. 69-75

Author(s):

Surabhi Nanda ◽

Sanjay Mishra ◽

V. P. Varshney ◽

Abhishek Tyagi ◽

R. B. Singh

Keyword(s):

Cervical Cancer ◽

Cell Line ◽

Cancer Cell ◽

Cytotoxic Effect ◽

Cancer Cell Line ◽

Cervical Cancer Cell ◽

Cervical Cancer Cell Line ◽

Human Cervical Cancer Cell ◽

Human Cervical Cancer

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HOXB4 inhibits the proliferation and tumorigenesis of cervical cancer cells by downregulating the activity of Wnt/β-catenin signaling pathway

Cell Death and Disease ◽

10.1038/s41419-021-03411-6 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Dan Lei ◽

Wen-Ting Yang ◽

Peng-Sheng Zheng

Keyword(s):

Cervical Cancer ◽

Cell Growth ◽

Signaling Pathway ◽

Cancer Cell ◽

Tumor Formation ◽

Cervical Cancer Cell ◽

Cancer Cell Growth ◽

Bioinformatics Analyses

AbstractHomeobox B4 (HOXB4), which belongs to the homeobox (HOX) family, possesses transcription factor activity and has a crucial role in stem cell self-renewal and tumorigenesis. However, its biological function and exact mechanism in cervical cancer remain unknown. Here, we found that HOXB4 was markedly downregulated in cervical cancer. We demonstrated that HOXB4 obviously suppressed cervical cancer cell proliferation and tumorigenic potential in nude mice. Additionally, HOXB4-induced cell cycle arrest at the transition from the G0/G1 phase to the S phase. Conversely, loss of HOXB4 promoted cervical cancer cell growth both in vitro and in vivo. Bioinformatics analyses and mechanistic studies revealed that HOXB4 inhibited the activity of the Wnt/β-catenin signaling pathway by direct transcriptional repression of β-catenin. Furthermore, β-catenin re-expression rescued HOXB4-induced cervical cancer cell defects. Taken together, these findings suggested that HOXB4 directly transcriptional repressed β-catenin and subsequently inactivated the Wnt/β-catenin signaling pathway, leading to significant inhibition of cervical cancer cell growth and tumor formation.

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