scholarly journals The effect of maternal sleep deprivation on differentiation of mesenchymal stem cells in the presence of neonates brain cerebrospinal fluid of Wistar rats

2020 ◽  
Vol 121 (02) ◽  
pp. 164-169
Author(s):  
S. H. Shariat ◽  
K. Parivar ◽  
N. H. Roudbari ◽  
M. Nabiuni
Keyword(s):  
Stem Cells ◽  
Cerebrospinal Fluid ◽  
Mesenchymal Stem Cells ◽  
Sleep Deprivation ◽  
Wistar Rats ◽  
Maternal Sleep ◽  
Maternal Sleep Deprivation

Maternal Sleep Deprivation Alters Reproductive Capability of Male Offspring in Wistar Rats

2016 ◽  
Vol 9 (2) ◽  
pp. 1-11
Author(s):  
Opeyemi Akindele ◽  
Olufadekemi Kunle-Alabi ◽  
Yinusa Raji
Keyword(s):  
Sleep Deprivation ◽  
Wistar Rats ◽  
Male Offspring ◽  
Maternal Sleep ◽  
Reproductive Capability ◽  
Maternal Sleep Deprivation

The Significant Effect of Conditioned Medium of Umbilical Cord Mesenchymal Stem Cells in Histological Improvement of Cartilage Defect in Wistar Rats

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Bintang SOETJAHJO ◽  
Mohammad HİDAYAT ◽  
Hidayat SUJUTİ ◽  
Yuda Heru FİBRİANTO
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Umbilical Cord ◽  
Cartilage Defect ◽  
Wistar Rats ◽  
Histological Improvement

Evaluation of the ability of collagen–glycosaminoglycan scaffolds with or without mesenchymal stem cells to heal bone defects in Wistar rats

2011 ◽  
Vol 16 (1) ◽  
pp. 47-55 ◽  
Author(s):  
M. Alhag ◽  
E. Farrell ◽  
M. Toner ◽  
T. Clive Lee ◽  
F. J. O’Brien ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Wistar Rats ◽  
Bone Defects

Pioglitazone alleviates maternal sleep deprivation-induced cognitive deficits in male rat offspring by enhancing microglia-mediated neurogenesis

2020 ◽  
Vol 87 ◽  
pp. 568-578 ◽  
Author(s):  
Yue Han ◽  
Jiutai Wang ◽  
Qiuying Zhao ◽  
Xiaofang Xie ◽  
Rui Song ◽  
...  
Keyword(s):  
Sleep Deprivation ◽  
Cognitive Deficits ◽  
Male Rat ◽  
Rat Offspring ◽  
Maternal Sleep ◽  
Maternal Sleep Deprivation

scholarly journals Modulation of gene transcription promoted by mesenchymal stem cells on cation-chloride cotransporter NKCC1 in experimental epilepsy

2021 ◽  
Author(s):  
Giovani Zocche Junior ◽  
Isadora Ghilardi ◽  
Laura Provenzi ◽  
Gabriel Leal ◽  
Giulia Pinzetta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Wistar Rats ◽  
Brain Structures ◽  
Treated Group ◽  
Control Group ◽  
Neural Firing ◽  
Post Transplantation ◽  
Neuroprotective Effects ◽  
Invasive Approach

Introduction: temporal lobe epilepsy is a disorder in which synchronized and rhythmic neural firing causes spontaneous recurrent seizures (1). Refractoriness due to this condition reaches 30% of its carriers (2,3). The search for therapeutic alternatives to help cope with this disease are extremely important. Mesenchymal stem cells (MSCs) appear as a plausible treatment option, as they present a less invasive approach and due to their niche modulating character (4,5). Objectives: this study aimed to quantify the gene expression of cation-chloride cotransporter NKCC1 encoded by the SLC12A2 gene in the encephalic tissue of pilocarpine-induced epileptic rats (6,7). Design: experimental study, brain institute of Rio Grande do Sul. Methods: MSCs were obtained from the bone marrow of Wistar rats, cultured, and transplanted through intravenous injection into control and epileptic Wistar rats. The rats were divided between control group, MSCs treated group, and pilocarpine group, containing 8 individuals each (8). Expression analysis was performed using real-time polymerase chain reaction. Results: for both 1 day and 7 days post-transplantation, an increase in the NKCC1 expression in both control and epileptic treated groups as compared to its expression in untreated epileptic and control groups with special attention to the amygdala, the hippocampus and the prefrontal cortex. Conclusion: MSCs stimulated expression of NKCC1 in brain structures of rats induced by pilocarpine to epilepsy. This corroborates the hypothesis of neuroprotective effects and modulating properties of stem cells and may point to more mechanisms for investigating the functioning and collaboration of these cells as a treatment for epilepsy.

scholarly journals P0678RENAL SUBCAPSULAR ADMINISTRATION OF ADIPOSE DERIVED MESENCHYMAL STEM CELLS PREVENTED THE PROGRESSION OF RENAL DAMAGE IN AN EXPERIMENTAL MODEL OF CKD

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Marina P Claro ◽  
Krislley R Pereira ◽  
Everidiene K V B Silva ◽  
Flavio Teles ◽  
Paulyana F Barbosa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Renal Function ◽  
Adipose Tissue ◽  
Survival Rate ◽  
Renal Fibrosis ◽  
Wistar Rats ◽  
Tubulointerstitial Fibrosis ◽  
Renal Inflammation ◽  
Male Wistar Rats

Abstract INTRODUCTION / AIMS Chronic kidney disease (CKD) is a progressive, debilitating condition of high lethality, which prevalence have been increasing considerably in recent decades. CKD can be triggered by many different factors, such as genetic predisposition, systemic hypertension, diabetes mellitus and autoimmune diseases. It is characterized by the gradual loss of renal function, leading to kidney failure and the need for renal replacement therapy for the maintenance of life. Regardless of the etiology of CKD, the establishment of local renal inflammation, with leucocyte recruitment, cell proliferation, extracellular matrix accumulation, glomerular and tubulointerstitial fibrosis, contribute significantly to its establishment and evolution. Due to its known pathophysiology, the primary aim when clinically treating CKD is to slow the progression of renal function loss and the advance of inflammation. However, until the present moment, there is no efficient pharmacological treatment to completely arrest the aggravation of renal inflammation and, specially, renal fibrosis. This motivates the scientific community to develop experimental research in order to test new therapeutic approaches to stunt renal fibrosis. In this context, experimental application of mesenchymal stem cells (mSC) as a treatment to control renal inflammation have been showing promising results in studies with animal models of CKD. The aim of the present study was to analyze the renoprotective effects of subcapsular application of Adipose Tissue-derived mSC (ASC), in rats submitted to 5/6 nephrectomy, after the establishment of the disease (15 days after CKD induction), in order to more closely resemble the clinical settings in humans. METHODS ASC were obtained from gonadal adipose tissue from healthy male Wistar rats. These cells were cultured until P4 when characterization by flow cytometry and in vitro differentiation were performed. Male Wistar rats underwent 5/6 nephrectomy and were followed for 15 days until the complete establishment of CKD (group CKD 15d). At this time, animals underwent a new surgery in which they received a subcapsular injection of 2x106 ASC diluted in 10 μL of sterile PBS (group CKD + ASC 30d), or only 10 μL of sterile PBS (group CKD 30d). Sham-operated rats, euthanized at day 15 (Sham 15d) and 30 (Sham 30d) were used as controls. Survival rate, body weight (BW), 24h urinary protein (24h UPE) and albumin (24h UAE) excretion serum creatinine (SCr) and blood urea nitrogen (BUN) concentration, percentage (GS%) and index (GSI) of glomerulosclerosis, tubulointerstitial fibrosis (INT%) and renal infiltration by macrophages (CD68) were studied at 15 and 30 days after 5/6 nephrectomy. Our results are presented as Mean ± SE. Differences among groups were analyzed by one-way ANOVA. RESULTS ASC injection significantly improved the survival rate of CKD + ASC 30d animals, compared to the observed in the untreated group. Moreover, ASC treatment markedly reduced protein and albumin urinary excretion, prevented the development of glomerulosclerosis, both the percentage of sclerotic glomeruli and the index of glomerular damage, numerically reduced interstitial fibrosis and significantly avoided renal inflammation by halting the progression of renal cortical macrophage infiltration. CONCLUSIONS According to our results, subcapsular ASC application promoted considerable renoprotection in the 5/6 renal ablation model, even after the complete establishment of severe CKD, suggesting that experimental therapy with these cells could be associated to the current pharmacological treatments employed to detain the progression of CKD. Figure

Low power laser and LED irradiation effect on proliferation and differentiation of Wistar rats mesenchymal stem cells

2013 ◽  
Author(s):  
Diana Mancera ◽  
Efrain Solarte ◽  
Leonardo Fierro ◽  
William Criollo
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Low Power ◽  
Wistar Rats ◽  
Irradiation Effect ◽  
Power Laser ◽  
Proliferation And Differentiation ◽  
Led Irradiation
Sign in / Sign up

Export Citation Format

Share Document