In Vitro and In Vivo Photoprotective Effects of (-)-Loliode Isolated from the Brown Seaweed, Sargassum horneri

Skin is the largest organ of humans. Overexposure to ultraviolet (UV) is the primary environmental factor that causes skin damage. The compound, (-)-loliode, isolated from the brown seaweed Sargassum horneri, showed strong antioxidant and anti-inflammatory activities in in vitro and in vivo models. To further explore the potential of (-)-loliode in cosmetics, in the present study, we investigated the photoprotective effect of (-)-loliode in vitro in skin cells and in vivo in zebrafish. The results indicated that (-)-loliode significantly reduced intracellular reactive oxygen species (ROS) level, improved cell viability, and suppressed apoptosis of UVB-irradiated human keratinocytes. In addition, (-)-loliode remarkably attenuated oxidative damage, improved collagen synthesis, and inhibited matrix metalloproteinases expression in UVB-irradiated human dermal fibroblasts. Furthermore, the in vivo test demonstrated that (-)-loliode effectively and dose-dependently suppressed UVB-induced zebrafish damage displayed in decreasing the levels of ROS, nitric oxide, lipid peroxidation, and cell death in UVB-irradiated zebrafish. These results indicate that (-)-loliode possesses strong photoprotective activities and suggest (-)-loliode may an ideal ingredient in the pharmaceutical and cosmeceutical industries.

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The Potential of Sulfated Polysaccharides Isolated from the Brown Seaweed Ecklonia maxima in Cosmetics: Antioxidant, Anti-melanogenesis, and Photoprotective Activities

Antioxidants ◽

10.3390/antiox9080724 ◽

2020 ◽

Vol 9 (8) ◽

pp. 724

Author(s):

Lei Wang ◽

Thilina U. Jayawardena ◽

Hye-Won Yang ◽

Hyo-Geun Lee ◽

You-Jin Jeon

Keyword(s):

Brown Seaweed ◽

Human Keratinocytes ◽

Vero Cells ◽

Dermal Fibroblasts ◽

Sulfated Polysaccharides ◽

Ecklonia Maxima ◽

B16f10 Cells ◽

Hdf Cells

Sulfated polysaccharides prepared from marine algae are potential ingredients in nutraceutical, pharmaceutical, and cosmeceutical industries. In the present study, the antioxidant, anti-melanogenesis, and photoprotective effects of sulfated polysaccharides obtained from Ecklonia maxima (EMC) were investigated to evaluate their potential in cosmetic. EMC was successfully prepared through Celluclast-assisted extraction and ethanol precipitation, and it contained 79.88% of sulfated polysaccharides that with 69.37% carbohydrates and 10.51% sulfate. EMC effectively suppressed 2,2-azobis(2-amidinopropane) hydrochloride (AAPH)-induced oxidative stress in vitro in Vero cells and in vivo in zebrafish. Furthermore, EMC significantly inhibited mushroom tyrosinase and reduced melanin synthesis in alpha-melanocyte-stimulating hormone-stimulated B16F10 cells. In addition, EMC remarkably attenuated photodamage induced by UVB irradiation in vitro in human keratinocytes (HaCaT cells) and in vivo in zebrafish. Furthermore, EMC effectively inhibited wrinkle-related enzymes and improved collagen synthesis in UVB-irradiated human dermal fibroblasts (HDF cells). These results indicate that EMC possesses strong antioxidant, anti-melanogenesis, and photoprotective activities, and suggest that EMC may be an ideal ingredient in the pharmaceutical and cosmeceutical industries.

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Essential Oil Microcapsules Immobilized on Textiles and Certain Induced Effects

Materials ◽

10.3390/ma12122029 ◽

2019 ◽

Vol 12 (12) ◽

pp. 2029 ◽

Cited By ~ 2

Author(s):

Miruna S. Stan ◽

Laura Chirila ◽

Alina Popescu ◽

Denisa M. Radulescu ◽

Diana E. Radulescu ◽

...

Keyword(s):

Membrane Integrity ◽

Dermal Fibroblasts ◽

Cell Membrane Integrity ◽

Textile Materials ◽

Skin Cells ◽

In Vitro Biocompatibility ◽

Short Term Exposure ◽

Before And After

In order to obtain textile materials with potential utility in the development of cosmetic textiles, this study examined the deposition by padding of rose and sage microcapsules on woven textile structures, with different fiber compositions (100% cotton and 50% cotton/50% polyester). Cationization of the textile materials was performed to enhance the degree of uptake the pf the microcapsules on the fabrics’ surface. A commercially acrylate-based binder was used to fix the microcapsules to the textile substrate and to improve the durability against external factors. The finished textile materials were characterized in terms of their physical-mechanical characteristics. The distribution of microcapsules on the fabrics surface before and after five washing cycles and 1000 abrasion cycles was investigated by scanning electron microscopy. The biocompatibility in terms of cell viability, cell membrane integrity and inflammation status of the functionalized fabrics was evaluated on CCD-1070Sk normal human dermal fibroblasts. The cell morphology was evaluated by F-actin staining using fluorescence microscopy and no significant changes were noticed after the incubation in the presence of fabrics compared with control. The in vitro biocompatibility evaluation on human skin cells confirmed the absence of cytotoxicity after the short-term exposure, supporting further in vivo use of these innovative textiles with improved properties.

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Topical Delivery of Carvedilol Loaded Nano-Transfersomes for Skin Cancer Chemoprevention

Pharmaceutics ◽

10.3390/pharmaceutics12121151 ◽

2020 ◽

Vol 12 (12) ◽

pp. 1151

Author(s):

Mengbing Chen ◽

Md Abdullah Shamim ◽

Ayaz Shahid ◽

Steven Yeung ◽

Bradley T. Andresen ◽

...

Keyword(s):

Targeted Delivery ◽

Cancer Chemoprevention ◽

Tween 80 ◽

Topical Delivery ◽

Human Keratinocytes ◽

Systemic Absorption ◽

Skin Damage ◽

Β Blocker

The β-blocker carvedilol has been shown to prevent skin carcinogenesis in vitro and in vivo. Since systemic absorption of the β-blocker may cause cardiovascular disturbance, we developed a carvedilol loaded transfersome for skin-targeted delivery. Transfersomes were prepared using phospholipids and surfactants at various ratios and characterized. One formulation (F18) selected for further analysis was composed of carvedilol, soy phosphatidylcholine, and Tween-80 at a ratio of 1:3:0.5, which had a particle size of 115.6 ± 8.7 nm, a zeta potential of 11.34 ± 0.67 mV, and an encapsulation efficiency of 93.7 ± 5.1%. F18 inhibited EGF-induced neoplastic transformation of mouse epidermal JB6 P+ cells at non-toxic concentrations, while only high concentrations induced cytotoxicity in JB6 P+ and human keratinocytes HaCaT. Compared to the free drug, F18 released through the dialysis membrane and permeated through the porcine ear skin at a slower rate, but similarly depositing the drug in the epidermis and dermis of the skin. Consistently, surface application of F18 on reconstructed full-thickness human skin showed slower drug permeation, while it suppressed ultraviolet-induced DNA damage, inflammatory gene expression, and apoptosis. These data indicate that transfersome is a promising topical delivery system of carvedilol for preventing ultraviolet-induced skin damage and carcinogenesis.

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Luteolin Prevents UVB-Induced Skin Photoaging Damage by Modulating SIRT3/ROS/MAPK Signaling: An in vitro and in vivo Studies

Frontiers in Pharmacology ◽

10.3389/fphar.2021.728261 ◽

2021 ◽

Vol 12 ◽

Author(s):

Jing Mu ◽

Huisheng Ma ◽

Hong Chen ◽

Xiaoxia Zhang ◽

Mengyi Ye

Keyword(s):

Animal Experiments ◽

Mapk Signaling ◽

Dermal Fibroblasts ◽

In Vivo Studies ◽

Uvb Radiation ◽

Skin Damage ◽

Cellular Protection ◽

Uvb Irradiation

The aim of this study was to investigate the role of luteolin in the mechanism of ultraviolet radiation B (UVB)-induced photoaging. An in vivo photoaging model was established using UVB irradiation of bare skin on the back of rats, and an in vitro photoaging model was established using UVB irradiation of human dermal fibroblasts (HDF). Skin damage was observed using hematoxylin-eosin (HE) and Masson staining, skin and cellular reactive oxygen species (ROS) levels were detected by DHE and DCF fluorescent probes, mitochondrial membrane potential was detected by JC-1 staining, and protein expressions were detected by immunofluorescence and Western Blot. Results from animal experiments showed that luteolin reduced UVB-induced erythema and wrinkle formation. Results from cellular assays showed that luteolin inhibited UVB-induced decrease in cell viability. In addition, in vitro and in vivo experiments showed that luteolin reduced oxidative stress levels, decreased activation of matrix metalloproteinases (MMPs) and increased collagen expression. Continued cellular experiments using 3-TYP, an inhibitor of Sirtuin 3 (SIRT3), revealed a loss of cellular protection by luteolin and a decrease in collagen, suggesting that luteolin acts by targeting and promoting SIRT3. luteolin is involved in the protection of skin cells against UVB radiation-induced ageing via the SIRT3/ROS/mitogen-activated protein kinases (MAPK) axis and it may be a promising therapeutic agent for the prevention of UVB photoaging.

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Skin Antiaging Attributes of the Dendrobium Huoshanense Polysaccharides

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.19:176-180 ◽

2019 ◽

Vol 19 (2) ◽

pp. 176-180

Author(s):

Fang-Li Gu ◽

Xiao-Mei He ◽

Ren-Shu Huang

Keyword(s):

Raw Materials ◽

Skin Aging ◽

Inhibitory Effects ◽

Tyrosinase Inhibition ◽

In Vivo Models ◽

Skin Cells ◽

Compositional Changes ◽

Dendrobium Huoshanense

Skin aging is a normal process that can only be slowed down by the use of effective cosmetics. The aging of the skin involves structural and compositional changes in the skin cells as well as darkening. This process largely involves loss of hygroscopicity and moisture and increases in tyrosinase activity that catalyzes melanin synthesis. Herein, we have investigated the effect of the polysaccharides from Dendrobium huoshanense on hygroscopicity, moisturizing, and tyrosinase inhibition activity in in vitro and in vivo models. The results show increase in hygroscopicity, moisturizing effect, and inhibitory effects on tyrosinase. In conclusion, the polysaccharides from D. huoshanense may be excellent raw materials for the development of antiaging cosmetics.

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Anti-Inflammatory Effects of Alnus Sibirica Extract on In Vitro and In Vivo Models

Molecules ◽

10.3390/molecules25061418 ◽

2020 ◽

Vol 25 (6) ◽

pp. 1418

Author(s):

Jeongyoon Choi ◽

Sunghee Moon ◽

Hyemi Bae ◽

Young-Won Kim ◽

Yelim Seo ◽

...

Keyword(s):

Immunoglobulin E ◽

Skin Inflammation ◽

Skin Lesions ◽

Dermal Fibroblasts ◽

In Vivo Model ◽

Necrosis Factor Alpha ◽

In Vivo Models ◽

Anti Inflammatory

Alnus sibirica extracts (ASex) have long been used in Oriental medicine to treat various conditions. To provide a scientific basis for this application and the underlying mechanism, we investigated the anti-inflammatory effects of ASex in vitro and in vivo. The in vitro model was established using human dermal fibroblasts (HDFs) treated with inflammatory stimulants (lipopolysaccharide, tumor necrosis factor-alpha, interferon-gamma). Lactate dehydrogenase and reverse transcription-polymerase chain reaction showed that ASex inhibited the increased expression of acute-phase inflammatory cytokines. The in vivo model was established by inducing skin inflammation in NC/Nga mice via the repeated application of house dust mite (HDM) ointment to the ears and back of the mice for eight weeks. HDM application increased the severity of skin lesions, eosinophil/mast cell infiltration, and serum immunoglobulin E levels, which were all significantly decreased by ASex treatment, demonstrating the same degree of protection as hydrocortisone. Overall, ASex showed excellent anti-inflammatory effects both in vitro and in vivo, suggesting its potential as an excellent candidate drug to reduce skin inflammation.

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In Vitro Expansion of Keratinocytes on Human Dermal Fibroblast-Derived Matrix Retains Their Stem-Like Characteristics

Scientific Reports ◽

10.1038/s41598-019-54793-9 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 3

Author(s):

Chee-Wai Wong ◽

Catherine F. LeGrand ◽

Beverley F. Kinnear ◽

Radoslaw M. Sobota ◽

Rajkumar Ramalingam ◽

...

Keyword(s):

Dermal Fibroblast ◽

Human Keratinocytes ◽

Dermal Fibroblasts ◽

Collagen I ◽

Matrix Composition ◽

Major Influence ◽

Primary Human Keratinocytes ◽

Matrix Deposition

AbstractThe long-term expansion of keratinocytes under conditions that avoid xenogeneic components (i.e. animal serum- and feeder cell-free) generally causes diminished proliferation and increased terminal differentiation. Here we present a culture system free of xenogeneic components that retains the self-renewal capacity of primary human keratinocytes. In vivo the extracellular matrix (ECM) of the tissue microenvironment has a major influence on a cell’s fate. We used ECM from human dermal fibroblasts, cultured under macromolecular crowding conditions to facilitate matrix deposition and organisation, in a xenogeneic-free keratinocyte expansion protocol. Phospholipase A2 decellularisation produced ECM whose components resembled the core matrix composition of natural dermis by proteome analyses. Keratinocytes proliferated rapidly on these matrices, retained their small size, expressed p63, lacked keratin 10 and rarely expressed keratin 16. The colony forming efficiency of these keratinocytes was enhanced over that of keratinocytes grown on collagen I, indicating that dermal fibroblast-derived matrices maintain the in vitro expansion of keratinocytes in a stem-like state. Keratinocyte sheets formed on such matrices were multi-layered with superior strength and stability compared to the single-layered sheets formed on collagen I. Thus, keratinocytes expanded using our xenogeneic-free protocol retained a stem-like state, but when triggered by confluence and calcium concentration, they stratified to produce epidermal sheets with a potential clinical use.

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Dieckol, an Algae-Derived Phenolic Compound, Suppresses UVB-Induced Skin Damage in Human Dermal Fibroblasts and Its Underlying Mechanisms

Antioxidants ◽

10.3390/antiox10030352 ◽

2021 ◽

Vol 10 (3) ◽

pp. 352

Author(s):

Lei Wang ◽

Jun-Geon Je ◽

Hye-Won Yang ◽

You-Jin Jeon ◽

Seungheon Lee

Keyword(s):

Brown Seaweed ◽

Dermal Fibroblasts ◽

Mitogen Activated Protein Kinase ◽

Activator Protein 1 ◽

Human Dermal Fibroblasts ◽

Skin Damage ◽

Mitogen Activated Protein ◽

Underlying Mechanisms ◽

Hdf Cells

Ultraviolet (UV) irradiation is considered to be the primary environmental factor that causes skin damage. In the present study, we investigated the protective effect of dieckol (DK), a compound isolated from the brown seaweed Ecklonia cava, against UVB-induced skin damage in human dermal fibroblasts (HDF cells). The results indicated that DK effectively inhibited the activity of collagenase. DK remarkably reduced the intracellular reactive oxygen species level and improved the viability of UVB-irradiated HDF cells. Besides, DK significantly and dose-dependently improved collagen synthesis and inhibited intracellular collagenase activity in UVB-irradiated HDF cells. In addition, DK markedly reduced the expression of proinflammatory cytokines and matrix metalloproteinases. Further analyses revealed that these processes were mediated through the regulation of nuclear factor kappa B, activator protein 1, and mitogen-activated protein kinase signaling pathways in the UVB-irradiated HDF cells. In conclusion, these results indicate that DK possesses strong in vitro photoprotective effects and therefore has the potential to be used as an ingredient in the cosmeceutical industry.

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Visfatin Promotes Wound Healing Through the Activation of ERK1/2 and JNK1/2 Pathway

10.20944/preprints201810.0667.v1 ◽

2018 ◽

Author(s):

Byungcheol Lee ◽

Jisun Song ◽

Arim Lee ◽

Daeho Cho ◽

Tae Sung Kim

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Human Keratinocytes ◽

Dermal Fibroblasts ◽

Dependent Manner ◽

Vegf Expression ◽

And Migration ◽

Proliferation And Migration

Visfatin, a member of the adipokine family, plays an important role in many metabolic and stress responses. The mechanisms underlying the direct therapeutic effects of visfatin on wound healing have not been reported yet. In this study, we examined the effects of visfatin on wound healing in vitro and in vivo. Visfatin enhanced the proliferation and migration of human dermal fibroblasts (HDFs) and keratinocytes, and significantly increased the expression of wound healing-related vascular endothelial growth factor (VEGF) in vitro and in vivo. Treatment of HDFs with visfatin induced activation of both extracellular signal-regulated kinases 1 and 2 (ERK1/2) and c-Jun N-terminal kinases 1 and 2 (JNK1/2) in a time-dependent manner. Inhibition of ERK1/2 and JNK1/2 led to a significant decrease in visfatin-induced proliferation and migration of HDFs. Importantly, blocking VEGF with its neutralizing antibodies suppressed the visfatin-induced proliferation and migration of HDFs and human keratinocytes, indicating that visfatin induces the proliferation and migration of HDFs and human keratinocytes via increased VEGF expression. Moreover, visfatin effectively improved wound repair in vivo, which was comparable to the wound healing activity of epidermal growth factor (EGF). Taken together, we demonstrate that visfatin promotes the proliferation and migration of HDFs and human keratinocytes by inducing VEGF expression and can be used as a potential novel therapeutic agent for wound healing.

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Effect of Bioactive Phytochemicals from Phlomis viscosa Poiret on Wound Healing

Plants ◽

10.3390/plants8120609 ◽

2019 ◽

Vol 8 (12) ◽

pp. 609 ◽

Cited By ~ 3

Author(s):

Ludmila Yarmolinsky ◽

Arie Budovsky ◽

Leonid Yarmolinsky ◽

Boris Khalfin ◽

Vladimir Glukhman ◽

...

Keyword(s):

Wound Healing ◽

Beneficial Effect ◽

Chronic Wounds ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

In Vivo Models ◽

Bioactive Phytochemicals ◽

Pro Inflammatory Cytokines

Phlomis viscosa Poiret is an evergreen shrub growing in Israel, Turkey, Lebanon, and Syria with acknowledged pro-wound healing (WH) properties. In this study, we evaluated the pro-WH potential of selected compounds found in this plant. Among the pro-WH compounds (identified by us) was a combination of three chemicals—diosmin, 1-octen-3-ol, and himachala-2,4-diene which enhanced WH significantly both in in vitro and in vivo models. The determined phytochemicals combination could be used for the treatment of chronic wounds. The effect of the extracts, diosmin, 1-octen-3-ol on the secretion of pro-inflammatory cytokines, IL-6 (A) and IL-8 (B) by human dermal fibroblasts was significant (p < 0.001). In addition, the beneficial effect of extracts of P. viscosa and its phytochemicals on WH was evidenced by inhibiting the growth of several WH delaying microorganisms.

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