Evaluation of In Vitro Antidiabetic and Antioxidant Activities and Preliminary Phytochemical Screening of Leaf Extracts of Avicennia officinalis

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

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PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITIES OF LEAF EXTRACTS OF ALTERNANTHERA BRASILIANA (L). KUNTZE AND ALTERNANTHERA BETTZICKIANA REGEL.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i6.25136 ◽

2018 ◽

Vol 11 (6) ◽

pp. 266

Author(s):

Kasthuri O R ◽

Ramesh B

Keyword(s):

Free Radical ◽

Secondary Metabolites ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Phytochemical Screening ◽

Leaf Extracts ◽

Water Extracts ◽

Hydroethanolic Extract

Objectives: The present study was carried out to determine the phytochemical constituents and in vitro antioxidant activities of leaf extracts of Alternanthera brasiliana (L). Kuntze (A. brasiliana) and Alternanthera bettzickiana regel (A. bettzickiana).Methods: Preliminary phytochemical screening was performed in petroleum ether, chloroform, acetone, ethanol, hydroethanolic and water extracts of leaves of A. brasiliana and A. bettzickiana. The level of alkaloids, flavonoids, total phenolic content, tannins, Vitamin C, Vitamin E, GSH, and total proteins were determined in hydroethanolic, ethanol, and water extracts. Antioxidant activity of the hydroethanolic leaf extracts of A. brasiliana and A. bettzickiana were determined by 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical scavenging assay, nitric oxide scavenging assay, superoxide anion scavenging assay, ferric reducing antioxidant power assay, total antioxidant capacity, and reducing power assay.Results: The phytochemical screening of six different extracts of A. brasiliana and A. bettzickiana revealed the presence of various phytonutrients. Quantitative analysis of secondary metabolites in ethanol, hydroethanolic and water extracts of leaves of A. brasiliana and A. bettzickiana showed the presence of high amount of secondary metabolites in the hydroethanolic extract. In vitro, antioxidant assay of two plant extracts revealed that A. bettzickiana was more potent than A. brasiliana in scavenging free radicals.Conclusion: The different extracts from A. brasiliana and A. bettzickiana and specifically the hydroethanolic extract of A. bettzickiana revealed several properties such as rich source of phytonutrients, higher free radical scavenging properties, and significant antioxidant capabilities. Therefore, the bioactive compound should be isolated in future studies and could be used as a safe and serve as a potential source of natural antioxidants.

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AN IN VITRO STUDY OF ANTIOXIDANT CAPACITY AND RADICAL SCAVENGING EFFECT OF SPINACIA OLERACEA LEAF EXTRACT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i11.28021 ◽

2018 ◽

Vol 11 (11) ◽

pp. 324

Author(s):

Mrinalini C Damle ◽

Shivani K Jadhav

Keyword(s):

Leaf Extract ◽

Spinacia Oleracea ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Phytochemical Screening ◽

Bioactive Constituents ◽

Leaf Extracts

Objective: The study was carried out to evaluate the preliminary phytochemical screening and antioxidant activity of ethanolic extract of Spinacia oleracea (SO).Methods: The leaves of SO were shade dried, and the extract was prepared using solvent ethanol by Soxhlet extraction method. The preliminary phytochemical screening was carried out on the leaf extract of the plant. The total phenolic content and total flavonoids were estimated using Folin- Ciocalteu’s and aluminum chloride reagents, respectively. Antioxidant activities were studied using 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, hydrogen radical, lipid peroxidation, and phosphomolybdenum radical scavenging assays.Results: The preliminary phytochemical analysis revealed the presence of bioactive constituents such as phenols, alkaloids, flavonoids, saponins, and glycosides. As SO is a rich source of different bioactive component, it contains a considerable amount of flavonoids and phenols. The different antioxidant assays proved that spinach is one of the best antioxidants with its ability to scavenge different radicals that generate oxidative stress.Conclusion: The observed activity may be associated with bioactive components such as phenols and flavonoids present in the leaf extracts and could have greater importance as nootropic plant in oxidative stress-related degenerative diseases such as Alzheimer and dementia.

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Preliminary Phytochemical Screening, In Vitro Antidiabetic, Antioxidant Activities, and Toxicity of Leaf Extracts of Psychotria malayana Jack

Plants ◽

10.3390/plants10122688 ◽

2021 ◽

Vol 10 (12) ◽

pp. 2688

Author(s):

Tanzina Sharmin Nipun ◽

Alfi Khatib ◽

Qamar Uddin Ahmed ◽

Mohd Hamzah Mohd Nasir ◽

Farahaniza Supandi ◽

...

Keyword(s):

Methanol Extract ◽

Antioxidant Activities ◽

Phytochemical Screening ◽

Leaf Extracts ◽

Isolation And Identification ◽

Therapeutic Concentration ◽

Water Extracts ◽

Methanol Extracts ◽

Insulin Sensitizing Activity

Psychotria malayana Jack belongs to the Rubiacea and is widespread in Southeast Asian countries. It is traditionally used to treat diabetes. Despite its potential medicinal use, scientific proof of this pharmacological action and the toxic effect of this plant are still lacking. Hence, this study aimed to investigate the in vitro antidiabetic and antioxidant activities, toxicity, and preliminary phytochemical screening of P. malayana leaf extracts by gas chromatography-mass spectrometry (GC-MS) after derivatization. The antidiabetic activities of different extracts of this plant were investigated through alpha-glucosidase inhibitory (AGI) and 2-NBDG glucose uptake using 3T3-L1 cell line assays, while the antioxidant activity was evaluated using DPPH and FRAP assays. Its toxicological effect was investigated using the zebrafish embryo/larvae (Danio rerio) model. The mortality, hatchability, tail-detachment, yolk size, eye size, beat per minute (BPM), and body length were taken into account to observe the teratogenicity in all zebrafish embryos exposed to methanol extract. The LC50 was determined using probit analysis. The methanol extract showed the AGI activity (IC50 = 2.71 ± 0.11 μg/mL), insulin-sensitizing activity (at a concentration of 5 µg/mL), and potent antioxidant activities (IC50 = 10.85 μg/mL and 72.53 mg AAE/g for DPPH and FRAP activity, respectively). Similarly, the water extract exhibited AGI activity (IC50 = 6.75 μg/mL), insulin-sensitizing activity at the concentration of 10 μg/mL, and antioxidant activities (IC50 = 27.12 and 33.71 μg/mL for DPPH and FRAP activity, respectively). The methanol and water extracts exhibited the LC50 value higher than their therapeutic concentration, i.e., 37.50 and 252.45 µg/mL, respectively. These results indicate that both water and methanol extracts are safe and potentially an antidiabetic agent, but the former is preferable since its therapeutic index (LC50/therapeutic concentration) is much higher than for methanol extracts. Analysis using GC-MS on derivatized methanol and water extracts of P. malayana leaves detected partial information on some constituents including palmitic acid, 1,3,5-benzenetriol, 1-monopalmitin, beta-tocopherol, 24-epicampesterol, alpha-tocopherol, and stigmast-5-ene, that could be a potential target to further investigate the antidiabetic properties of the plant. Nevertheless, isolation and identification of the bioactive compounds are required to confirm their antidiabetic activity and toxicity.

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Phytochemical analysis and salivary amylase inhibition activities of Carica papaya leaf and Garcinia mangostana pericarp extracts and partially purified fractions

International Journal of Pharmaceutical and Phytopharmacological Research ◽

10.24896/eijppr.2016616 ◽

2017 ◽

Vol 6 (1) ◽

pp. 34

Author(s):

Michael Russelle Alvarez ◽

Paolo Robert Bueno ◽

Raymond Oliver Cruz ◽

Richard Macapulay ◽

Francis Jayson Vallesfin ◽

...

Keyword(s):

Crude Extract ◽

Carica Papaya ◽

Uv Light ◽

Digestive Enzyme ◽

Phytochemical Analysis ◽

Phytochemical Screening ◽

Salivary Amylase ◽

Garcinia Mangostana ◽

Leaf Extracts

Plant-derived digestive enzyme inhibitors particularly those targeted to carbohydrate metabolism has been the focus of recent studies as natural supplements for weight control and diabetes. The present study explores the salivary amylase inhibition activity of Garcinia mangostana (Linn.) pericarp extracts and Carica papaya (Linn.) leaf extracts and fractions, as well as perform phytochemical screening and quantification, and thin layer – and high performance liquid chromatographic profiling. Results show that crude extracts and purified fractions were able to inhibit salivary amylase, with C. papaya fraction 1 being the most active at 30.89% inhibition. Phytochemical screening of all extracts tested positive for tannins, glycosides, phenolics, flavonoids and alkaloids. Quantification of phenolics showed that extracts contained high levels of phenolics, with C. papaya crude extract having the highest content with 219.0±12.7 mg GAE/g extract followed by G. mangostana crude extract with 247.1±18.0 mg GAE/g extract. Quantification of total flavonoids also showed C. papaya crude extract to contain the highest content with 55.12±0.679 mg QE/g extract. All extracts contained negligible alkaloid content, though. HPLC and TLC profiling showed several peaks and bands, when viewed in 210 nm and UV light, respectively. These results demonstrate in vitro the salivary amylase inhibitory activity of both plants and their potential as antidiabetic drug candidates; however, further studies need to be done, like isolation and structure elucidation of active components and toxicity assays. Keywords: Amylase inhibition, phytochemical quantification, Carica papaya, Garcinia mangostana

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Phytochemical Screening, Antioxidant Activities and In Vitro Anticancer Potential of Egyptian Capsicum Spp.

Biochemistry & Pharmacology Open Access ◽

10.4172/2167-0501.1000205 ◽

2015 ◽

Vol 05 (02) ◽

Cited By ~ 1

Author(s):

Shaimaa GA ◽

Mahmoud MS

Keyword(s):

Antioxidant Activities ◽

Phytochemical Screening ◽

Capsicum Spp

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DETECTION OF BIOACTIVE FRACTIONS OF JUSTICIA ADHATODA L. LEAVES

Canadian Journal of Applied Sciences ◽

10.21065/19257430.388.3 ◽

2013 ◽

Vol 3 ◽

pp. 388

Author(s):

Faiza Rasheed ◽

Keyword(s):

Crude Oil ◽

Metal Ions ◽

Dietary Fiber ◽

Total Alkaloid ◽

Antioxidant Activities ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Essential Metal ◽

Justicia Adhatoda

In vitro antibacterial and antioxidant activities of various leaf extracts of Justicia adhatoda L. (locally known as Bhaikar) were assessed. The leaves were also subjected to various phytochemical analysis. Results revealed that leaves of J. adhatoda L. contain significant amount of total alkaloid, phenols flavonoid, saponins, tannins , protein, crude oil, dietary fiber, essential and non essential metal ions. The methanol, ethanol, butanol, chloroform and n-hexane leaf extracts of J

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In vitro antioxidant activity and phytochemical analyses of Acalypha godseffiana (Euphobiaceae) leaf extracts

Nigerian Journal of Natural Products and Medicine ◽

10.4314/njnpm.v25i1.8 ◽

2021 ◽

Vol 25 (1) ◽

pp. 75-79

Author(s):

S.O. Olubodun ◽

G.E. Eriyamremu ◽

M.E. Ayevbuomwan ◽

C.I. Nzoputa

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Antioxidant Vitamin ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Anti Oxidant ◽

In Vitro Antioxidant Activity

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

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