A Novel Role of Adiponectin: Stimulator of Human SW480 Colon Carcinoma Cell Migration by Inducing an Autocrine IL-8 and MCP-1 Loop

Selectins and fibrin(ogen) play key roles in the hematogenous dissemination of tumor cells, and especially of colon carcinomas. However, the fibrin(ogen) receptor(s) on colon carcinoma cells has yet to be defined along with its relative capacity to bind fibrinogen versus fibrin under flow. Moreover, the functional P-selectin ligand has yet to be validated using intact platelets rather than purified selectin substrates. Using human CD44-knockdown and control LS174T cells, we demonstrate the pivotal involvement of CD44 in the P-selectin-mediated binding to platelets in shear flow. Quantitative comparisons of the binding kinetics of LS174T versus P-selectin glycoprotein ligand-1 (PSGL-1)-expressing THP-1 cells to activated platelets reveal that the relative avidity of P-selectin-CD44 binding is more than sevenfold lower than that of P-selectin-PSGL-1 interaction. Using CD44-knockdown LS174T cells and microspheres coated with CD44 immunoprecipitated from control LS174T cells, and purified fibrin(ogen) as substrate, we provide the first direct evidence that CD44 also acts as the major fibrin, but not fibrinogen, receptor on LS174T colon carcinoma cells. Interestingly, binding of plasma fibrin to CD44 on the colon carcinoma cell surface interferes with the P-selectin-CD44 molecular interaction and diminishes platelet-LS174T heteroaggregation in the high shear regime. Cumulatively, our data offer a novel perspective on the apparent metastatic potential associated with CD44 overexpression on colon carcinoma cells and the critical roles of P-selectin and fibrin(ogen) in metastatic spread and provide a rational basis for the design of new therapeutic strategies to impede metastasis.

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Tumor cell migration is not influenced by p21 in colon carcinoma cell lines after irradiation with X-ray or 12C heavy ions

Radiation and Environmental Biophysics ◽

10.1007/s00411-010-0297-x ◽

2010 ◽

Vol 49 (3) ◽

pp. 427-435 ◽

Cited By ~ 8

Author(s):

Kristina Goetze ◽

Michael Scholz ◽

Gisela Taucher-Scholz ◽

Wolfgang Mueller-Klieser

Keyword(s):

Cell Migration ◽

Tumor Cell ◽

Colon Carcinoma ◽

Cell Lines ◽

Heavy Ions ◽

Carcinoma Cell ◽

X Ray ◽

Tumor Cell Migration ◽

Carcinoma Cell Lines

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The mycotoxin zearalenone enhances cell proliferation, colony formation and promotes cell migration in the human colon carcinoma cell line HCT116

Toxicology Letters ◽

10.1016/j.toxlet.2016.04.012 ◽

2016 ◽

Vol 254 ◽

pp. 1-7 ◽

Cited By ~ 24

Author(s):

Haila Abassi ◽

Imen Ayed-Boussema ◽

Sarah Shirley ◽

Salwa Abid ◽

Hassen Bacha ◽

...

Keyword(s):

Cell Proliferation ◽

Cell Migration ◽

Cell Line ◽

Colon Carcinoma ◽

Colony Formation ◽

Carcinoma Cell ◽

Human Colon ◽

Human Colon Carcinoma Cell ◽

Colon Carcinoma Cell Line ◽

Human Colon Carcinoma

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Brucine, an alkaloid from seeds of Strychnos nux-vomica Linn., represses hepatocellular carcinoma cell migration and metastasis: The role of hypoxia inducible factor 1 pathway

Toxicology Letters ◽

10.1016/j.toxlet.2013.07.024 ◽

2013 ◽

Vol 222 (2) ◽

pp. 91-101 ◽

Cited By ~ 26

Author(s):

Guangwen Shu ◽

Xue Mi ◽

Jian Cai ◽

Xinlin Zhang ◽

Wu Yin ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Cell Migration ◽

Carcinoma Cell ◽

Hypoxia Inducible Factor ◽

Hypoxia Inducible Factor 1 ◽

Hepatocellular Carcinoma Cell

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Inhibition of Colon Carcinoma Cell Migration Following Treatment with Purified Venom from Lesser Weever Fish (Trachinus Vipera)

Cellular Physiology and Biochemistry ◽

10.1159/000475646 ◽

2017 ◽

Vol 41 (6) ◽

pp. 2279-2288 ◽

Cited By ~ 2

Author(s):

Myriam Fezai ◽

Chaker Slaymi ◽

Mossadok Ben-Attia ◽

Guido Kroemer ◽

Florian Lang ◽

...

Keyword(s):

Cell Migration ◽

Colon Carcinoma ◽

Cell Shape ◽

Carcinoma Cell ◽

Tissue Injury ◽

Marked Change ◽

Human Colon ◽

Cell Area ◽

Migration Assay ◽

Cellular Effects

Background: Injury by the sting of Lesser weever fish (Trachinus vipera) may lead to severe pain, edema or tissue necrosis. Cellular effects of the venom are still incompletely understood. Previous observations revealed that purified Lesser weever fish venom (LWFV) induces suicidal death of erythrocytes and HCT116 human colon carcinoma cells. The present study addressed the effect of the venom on colon carcinoma cell toxicity, shape and migration both in p53+/+ and/or p53-/- conditions. Methods: Cells were exposed to medium without or with 500 µg/ ml LWFV. Cell shape, cell area and circularity were visualized and quantified by fluorescence microscopy. Cell volume, granularity and cells toxicity were assessed via the apoptotic parameters dissipation of mitochondrial inner transmembrane potential, phosphatidylserine surface exposure and cell membrane permeabilization were measured utilizing flow cytometry. Cell migration was evaluated using wound healing assay and two-dimensional migration assay. Results: LWFV treatment was followed by a marked change of cell shape and size, significant decrease of cell area and circularity, significant impairment of cell migration, as well as induction of apoptosis after long exposition. Conclusions: LWFV exposure leads to cell shrinkage, increased granularity, apoptosis and impairment of cell migration, effects presumably contributing to LWFV-induced tissue injury.

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