Body Surface Changes in Gastrointestinal Helminthes Following in vitro Treatment with Allium sativum Oil

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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The cost of obtaining improved planting material of garlic (Allium sativum L.) using the in vitro method

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/650/1/012050 ◽

2021 ◽

Vol 650 (1) ◽

pp. 012050

Author(s):

M A Azopkova ◽

I V Muravyeva ◽

A V Polyakov ◽

O A Razin

Keyword(s):

Allium Sativum ◽

In Vitro Method ◽

Planting Material ◽

The Cost

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Novel ε-polylysine/polyethyleneimine -coated Ag nanoparticles for in vitro treatment of Pseudomonas aeruginosa

Biochemical Engineering Journal ◽

10.1016/j.bej.2021.107937 ◽

2021 ◽

Vol 168 ◽

pp. 107937

Author(s):

Jingjing Zhang ◽

Feng Qu ◽

Taobo Liang ◽

Sidi Lv ◽

Wen Li ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Ag Nanoparticles ◽

In Vitro Treatment

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Impairment of mitochondrial oxidative phosphorylation in skin fibroblasts of SALS and FALS patients is rescued by in vitro treatment with ROS scavengers

Experimental Neurology ◽

10.1016/j.expneurol.2021.113620 ◽

2021 ◽

Vol 339 ◽

pp. 113620

Author(s):

Grazyna Debska-Vielhaber ◽

Irina Miller ◽

Viktoriya Peeva ◽

Werner Zuschratter ◽

Jaroslaw Walczak ◽

...

Keyword(s):

Oxidative Phosphorylation ◽

Skin Fibroblasts ◽

Mitochondrial Oxidative Phosphorylation ◽

Ros Scavengers ◽

In Vitro Treatment

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Prostaglandin F2α Represses IGF-I-Stimulated IRS1/Phosphatidylinositol-3-Kinase/AKT Signaling in the Corpus Luteum: Role of ERK and P70 Ribosomal S6 Kinase

Molecular Endocrinology ◽

10.1210/me.2009-0312 ◽

2010 ◽

Vol 24 (3) ◽

pp. 632-643 ◽

Cited By ~ 30

Author(s):

Edward Arvisais ◽

Xiaoying Hou ◽

Todd A. Wyatt ◽

Koumei Shirasuna ◽

Heinrich Bollwein ◽

...

Keyword(s):

Corpus Luteum ◽

Prostaglandin F2α ◽

Serine Phosphorylation ◽

Phosphatidylinositol 3 Kinase ◽

Akt Activation ◽

Diminished Capacity ◽

Igf I ◽

In Vitro Treatment

Abstract Little is known about the early intracellular events that contribute to corpus luteum regression. Experiments were designed to determine the effects of prostaglandin F2α (PGF2α) on phosphatidylinositol-3-kinase (PI3K)/Akt signaling in the corpus luteum in vivo and in vitro. Treatment of midluteal-phase cows with a luteolytic dose of PGF2α resulted in a rapid increase in ERK and mammalian target of rapamycin (mTOR)/p70 ribosomal protein S6 kinase (p70S6K1) signaling and a rapid suppression of Akt phosphorylation in luteal tissue. In vitro treatment of primary cultures of luteal cells with PGF2α also resulted in an increase in ERK and mTOR/p70S6K1 signaling and a diminished capacity of IGF-I to stimulate PI3K, Akt, and protein kinase C ζ activation. Accounting for the reductions in PI3K and Akt activation observed in response to PGF2α treatment, we found that PGF2α promoted the phosphorylation of serine residues (307, 612, 636) in the insulin receptor substrate 1 (IRS1) peptide sequence in vivo and in vitro. Serine phosphorylation of IRS1 was associated with reduced formation of IGF-I-stimulated IRS1/PI3Kp85 complexes. Furthermore, treatment with inhibitors of the MAPK kinase 1/ERK or mTOR/p70S6K1 signaling pathways prevented PGF2α-induced serine phosphorylation of IRS1 and abrogated the inhibitory actions of PGF2α on Akt activation. Taken together, these experiments provide compelling evidence that PGF2α treatment stimulates IRS1 serine phosphorylation, which may contribute to a diminished capacity to respond to IGF-I. It seems likely that the rapid changes in phosphorylation events are among the early events that mediate PGF2α-induced corpus luteum regression.

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Metallothionein in cultured human epithelial cells and synovial rheumatoid fibroblasts after in vitro treatment with auranofin

Biochemical Pharmacology ◽

10.1016/0006-2952(86)90738-0 ◽

1986 ◽

Vol 35 (12) ◽

pp. 2033-2040 ◽

Cited By ~ 14

Author(s):

Anne Glennås ◽

Peter E. Hunziker ◽

Justine S. Garvey ◽

Jeremias H.R. Kägi ◽

Hans E. Rugstad

Keyword(s):

Epithelial Cells ◽

Human Epithelial Cells ◽

In Vitro Treatment

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The effect of garlic extract (Allium sativum L.) (Amaryllidaceae) to eradicate the Porphyromonas endodontalis biofilm: An in-vitro research

Scientific Dental Journal ◽

10.4103/sdj.sdj_79_21 ◽

2021 ◽

Vol 5 (3) ◽

pp. 138

Author(s):

AdiDharma Widjaya ◽

MeinyFaudah Amin ◽

Aryadi ◽

BoediOetomo Roeslan

Keyword(s):

Allium Sativum ◽

Garlic Extract

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Lysophosphatidylcholine and sodium-calcium exchange in cardiac sarcolemma: comparison with ischemia

AJP Cell Physiology ◽

10.1152/ajpcell.1991.260.3.c433 ◽

1991 ◽

Vol 260 (3) ◽

pp. C433-C438 ◽

Cited By ~ 17

Author(s):

M. M. Bersohn ◽

K. D. Philipson ◽

R. S. Weiss

Keyword(s):

Phospholipase A2 ◽

Phospholipid Composition ◽

Maximum Velocity ◽

Passive Permeability ◽

Cardiac Sarcolemma ◽

Sodium Calcium ◽

Calcium Exchange ◽

Sarcolemmal Vesicles ◽

In Vitro Treatment

Lysophosphoglyceride accumulation in ischemic myocardium has been hypothesized to be a mechanism for altered sarcolemmal properties that underlie electrophysiological changes and Ca2+ accumulation in ischemia. We find that in vitro application of lysophosphatidylcholine to normal canine sarcolemmal vesicles at a concentration of 0.3 mumol/mg sarcolemmal protein inhibits Na(+)-Ca2+ exchange. Both maximum velocity (Vmax) for Ca2+ transport and Ca2+ affinity are reduced by lysophosphatidylcholine, whereas in ischemia only Vmax is reduced [M. M. Bersohn, K. D. Philipson, and J. Y. Fukushima. Am. J. Physiol. 242 (Cell Physiol. 11): C288-C295, 1982]. This amount of lysophosphatidylcholine does not affect sarcolemmal passive permeability to either Ca2+ or Na+. Treatment of sarcolemma with phospholipase A2 sufficient to inhibit Na(+)-Ca2+ exchange velocity by 50% causes large increases in sarcolemmal lysophosphatidylcholine and lysophosphatidylethanolamine. On the other hand, 1 h of ischemia in rabbit hearts does not affect sarcolemmal phospholipid composition. Thus, although in vitro treatment with lysophosphatidylcholine or phospholipase A2 has profound effects on sarcolemmal properties, sarcolemmal accumulation of lysophosphatidylcholine cannot account for the effects of ischemia as measured in highly purified sarcolemmal vesicles from ischemic hearts.

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