Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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Aktivitas Antijamur Air Perasan Daun Seledri (Apium graveolens L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v4i2.2010.102-107 ◽

2017 ◽

Vol 4 (2) ◽

pp. 102

Author(s):

Patra Inova Ardelia ◽

Fauzia Andrini ◽

M Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Fungicidal Activity ◽

Negative Control ◽

Apium Graveolens ◽

Diffusion Method ◽

Randomized Design ◽

Significant Difference ◽

Post Hoc

Celery (Apium graveolens L.) has been known as traditional medicine. Apigenin is one of active component in Celeryleaf that has fungicidal activity, so celery leaf’s extortion suspected has fungicidal activity to Candida albicans. Apigenincan solved in water. The aim of this research was to know antifungal activity of celery leaf’s extortion againsts Candidaalbicans in vitro.This is a laboratory experimental research by using completely randomized design and disc diffusion method. Celeryleaf’s extortion was divided into 3 concentrations, there were 25%, 50% and 100%. Ketoconazole 2% was used aspositive control and aquadest as negative control. The result of this research was based on analysis of variance, therewas significant difference between minimal 2 treatment (p=0.000) and confirmed with Post Hoc test, there were significantdifference in all treatments. As the conclusion, celery leaf’s extortion had antifungal activity againsts Candida albicansin vitro with the best concentration 50% but smaller than ketoconazole 2%.

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Aktivitas Antifungi Ekstrak Etanol Daun Eryngium foetidum L. Terhadap Pertumbuhan Xeromyces sp. secara in vitro

Jurnal Protobiont ◽

10.26418/protobiont.v8i2.34056 ◽

2019 ◽

Vol 8 (2) ◽

Author(s):

Ines S Simatupang Elvi Rusmiyanto PW, Rikhsan Kurniatuhadi

Keyword(s):

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Activity Level ◽

Piper Nigrum ◽

Dilution Method ◽

Randomized Design ◽

Eryngium Foetidum ◽

Completely Randomized Design

Eryngium foetidum is a plant commonly used a seasoning cuisine and has a variety of chemical compounds that potentially an antifungal. The ethanol extract Eryngium foetidum was tested against fungal isolate Xi.01. The isolat Xi.01 isolated from the pepper (Piper Nigrum) stem was identified as Xeromyces sp. This study aimed to determine the ability of the antifungal ethanol extract of Eryngium foetidum against Xeromyces sp. (Xi.01). This study used solid dilution method and completely randomized design using 18 treatments, i.e negative control, positive control, diethanolamide concentration of 2.5; 5; 7.5 and 10% combined with the ethanol extract concentration of Eryngium foetidum of 5; 10; 20 and 40%. The results showed 16 treatment combinations had the same low antifungal activity level in the range 13,59-22,40%

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Effectivity of Trichoderma harzianum as Bio-fungicide Against Moler Disease and Bio-stimulator of Shallot Growth

Journal of Agri-Food Science and Technology ◽

10.12928/jafost.v1i1.1941 ◽

2020 ◽

Vol 1 (1) ◽

pp. 12

Author(s):

Icha Rusita ◽

Hadi Sasongko

Keyword(s):

Trichoderma Harzianum ◽

Dry Weight ◽

Positive Control ◽

Negative Control ◽

Correlation Pattern ◽

Randomized Design ◽

Significant Difference ◽

Wet Weight ◽

Number Of Leaves ◽

Completely Randomized Design

Moler disease is the primary disease that disturbs the shallot’ cultivation. Hence, it is beneficial to have preventive measures to mitigate the risk and to improve growth. The research objective is to know the effectivity of Trichoderma harzianum suspension as a bio-fungicide and bio-stimulator. The study used seven treatments, i.e., immersion of shallot seeds on negative control (distilled water), positive control (synthetic fungicide+ZPT 1g/liter), T.harzianum suspension with different concentrations of 2 ppm, 4 ppm, 6 ppm, 8 ppm, and 10 ppm, assigned in a Completely Randomized Design (CRD). Treatment that results in a significant level will be continued to the Least Significance Different (LSD) 5% test. The observed variables are the intensity of moler disease and the growth of shallot seeds. Experiment results show the significant difference between treatments. The optimal treatment to reduce the percentage of moler disease intensity is the immersion of shallot seeds in 10 ppm-concentrated T.harzianum suspension, while to improve the shallot growth is in 8 ppm-concentrated T.harzianum suspension. The intensity of moler disease has a negative correlation to the plant’s height and leaf’s diameter but shows no correlation pattern to the number of leaves, wet weight, dry weight, and length of roots.

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PENGARUH EKSTRAK Sargassum cristaefolium PADA MULTIPLIKASI Dendrobium antennatum Rchb.f SECARA IN VITRO

Bioscientist : Jurnal Ilmiah Biologi ◽

10.33394/bjib.v8i1.2672 ◽

2020 ◽

Vol 8 (1) ◽

pp. 18

Author(s):

Faisal Ansyarif ◽

Mursal Ghazali ◽

Aida Muspiah ◽

Rina Kurnianingsih

Keyword(s):

Culture Media ◽

Positive Control ◽

Negative Control ◽

Growth Media ◽

Extract Concentration ◽

Tissue Culture Media ◽

Randomized Design ◽

Completely Randomized Design ◽

Natural Cytokinin

The purpose of this study was to determine the potential and concentration of Sargassum cristaefolium extract as a natural cytokinin in tissue culture media of Dendrobium antennatum Rchb.f. This study is experimental with a completely randomized design, using several extract concentrations compared with the positive control (BAP 1.5 ppm) and negative control (MS0 media). Extract concentrations used 5 ppm, 10 ppm, 15 ppm, 20 ppm, and 25 ppm. Based on analysis of variance (ANOVA), the effect of Sargassum cristaefolium extract on the growth media significant on all parameters. Sargassum cristaefolium extracts caused different responses at certain levels of concentration. Extract concentration of 10 ppm was able to initiate the highest number of shoots and leaves compared to other extract concentrations, where as the concentration 20 ppm was able to accelerate and increase root growth.

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Potensi Bacillus licheniformis dan Streptomyces olivaceoviridis sebagai Penghambat Pertumbuhan Jamur Saprolegnia sp. Penyebab Saprolegniasis pada Ikan secara In Vitro [Potential of Bacillus licheniformis and Streptomyces olivaceoviridis as Inhibiting The Growth of Fungus Saprolegnia sp, Cause Saprolegniasis on Fish by Using In Vitro]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v7i2.11196 ◽

2019 ◽

Vol 7 (2) ◽

pp. 133

Author(s):

Oktantia Frenny Anggani ◽

Rahayu Kusdarwati ◽

Hari Suprapto

Keyword(s):

Bacillus Licheniformis ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Streptomyces Olivaceoviridis ◽

Tukey Honestly Significant Difference

Abstract Saprolegniasis is a fungal disease on fish caused by Saprolegnia sp, which is saprophyte, damaging healthy tissue and makes the immune system in fish deacreased. The uniquennes of Saprolegnia sp has the main components of the cell wall in the form of chitin that was instrumental in shaping the structure of the tip growth of fungal hyphae. Control of fungal pathogenic Saprolegnia sp can use chitinolytic microorganisms based on ability to produce chitinase for example using bacteria. This study aims to potential of Bacillus licheniformis and Streptomyces olivaceoviridis as inhibiting the growth of fungus Saprolegnia sp, cause saprolegniasis on fish by using in vitro. The research method is experimental with completely randomized design (CRD), which consists of four treatments with five replications. The experimental used is A (Saprolegnia sp and Bacillus licheniformis), B (Saprolegnia sp and Streptomyces olivaceoviridis), C negative control (Saprolegnia sp) and D positive control (Saprolegnia sp and Ketokonazol 2 %). The main parameters measured were observed inhibition zone on each treatment. Supporting parameters were observed is an observation of abnormal hpyphae structure after being induced by bacteria Bacillus licheniformis dan Streptomyces olivaceoviridis. Data were analyzed using analysis of variants (ANOVA) and to know the difference between treatments were determined by Tukey honestly significant difference (Tukey HSD) Test. The results showed that the potential of chitinolytic Bacillus licheniformis can provide a good barrier of 4,62 cm by 5,48 cm compared Streptomyces olivaceoviridis in inhibiting the growth Saprolegnia sp. Suggestions in this research is the need to further research on the value of chitinase Bacillus licheniformis and Streptomyces olivaceoviridis. Further research by using in vivo.

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Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro

E3S Web of Conferences ◽

10.1051/e3sconf/202015101046 ◽

2020 ◽

Vol 151 ◽

pp. 01046

Author(s):

Henni Vanda ◽

Rizki Parindra ◽

Muhammad Hambal ◽

Farida Athaillah

Keyword(s):

Methanolic Extract ◽

Anthelmintic Activity ◽

Positive Control ◽

Negative Control ◽

Fasciola Gigantica ◽

Histopathological Study ◽

Randomized Design ◽

Completely Randomized Design ◽

Phosphate Buffered Saline

Fasciola gigantica is a parasite that causes a disruption of the metabolism of fats, proteins, and carbohydrates, which interferes growth and causes death. Curcuma aeruginosa Roxb extract is one of the medicinal plants which has been used to treat several diseases. The aim of this study was to determine the effect of methanolic extract of C. aeruginosa Roxb on F. gigantica, including mortality time and histopathological changes that occurred after treatment. This study used a completely randomized design with five replications. The flukes were soaked in three different extract concentrations: 10% (T1), 25% (T2), and 50% (T3) (w/v). Phosphate buffered saline (PBS) solution was used as a negative control (C1) and albendazole as the positive control (C2). The mortality time of F. gigantica in each group was calculated, and the dead flukes were prepared for histopathological study. The data were analyzed by Analysis of variance. The results showed that C. aeruginosa extracts at the concentration of 10, 25 and 50% caused the death of the flukes within 75, 57 and 48 minutes, respectively., Histopathological observations showed that the extract caused breakage of tegument which is an important organ in the respiratory process and nutrient absorption. This study concluded that C. aeruginosa extract exhibited anthelmintic activity towards F. gigantica in vitro.

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UJI EFEKTIVITAS EKSTRAK ETANOL DAUN PEGAGAN (Centella asiatica (L.) Urban) SEBAGAI ANTIDIABETES TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽

10.35799/pha.10.2021.32767 ◽

2021 ◽

Vol 10 (1) ◽

pp. 736

Author(s):

Grace Laury Tulung ◽

Widdhi Bodhi ◽

Jainer Pasca Siampa

Keyword(s):

Rattus Norvegicus ◽

Design Method ◽

Centella Asiatica ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Flavonoid Compound ◽

Randomized Design ◽

Decrease Blood Glucose ◽

Completely Randomized Design

ABSTRACT Gotu Kola Leaf (Centella asiatica (L.) Urban) are known to contain flavonoid compound, and flavonoid are known to decrease blood glucose level by stimulating pancreas beta cells to produce insulin. The aim of this research is to find out wether the ethanol extract of gotu kola leaf (Centella asiatica (L.) Urban) has the antidiabetic effectiveness or not. This research used Completely Randomized Design method. There were 15 rats used in this research that were split into 5 groups that is negative control (Aquadest), positive control (Metformin), and ethanol extract of gotu kola leaf with the dosage of 5.4 mg;10.8 mg;21.6 mg. The acquired data were analyzed with ANOVA test and LSD test. The analysis result showed that ethanol extract of gotu kola leaf had antidiabetic effectiveness against male white rat. Keywords: Centella asiatica (L.) Urban, antidiabetic, Rattus norvegicus ABSTRAK Daun Pegagan (Centella asiatica (L.) Urban) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan menurunkan kadar glukosa darah dengan merangsang sel beta pankreas untuk memproduksi insulin. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun pegagan memiliki efekivitas antidiabetes. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (aquadest), kontrol positif (metformin), dan ekstrak etanol daun pegagan dengan dosis 5,4 mg; 10,8 mg; 21,6 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menujukkan bahwa ekstrak etanol daun pegagan memiliki efektivitas antidiabetes terhadap tikus putih jantan (Rattus norvegicus). Kata kunci: Centella asiatica (L.) Urban, antidiabetes, Rattus norvegicus

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KECERNAAN IN VITRO SILASE SAMPAH SAYUR DAN DAUN GAMAL MENGGUNAKAN MIKROORGANISME RUMEN KAMBING

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v2i3.3808 ◽

2015 ◽

Vol 2 (3) ◽

pp. 17

Author(s):

Indrayani Indrayani ◽

Harapin Hafid ◽

Dian Agustina

Keyword(s):

Organic Matter ◽

Analysis Of Variance ◽

Dry Matter ◽

Vegetable Waste ◽

Dry Matter Digestibility ◽

Randomized Design ◽

Honestly Significant Difference ◽

Significant Difference ◽

Completely Randomized Design

ABSTRACTThis study aims to determine the digestibility of dry matter and organic matter level waste silage mixed vegetables and Gliricidia leaves were tested in vitro. This study used a completely randomized design with 3 treatments and 3 replications. Each of these treatments is R0 (Gliricidia leaves 100%), R1 (Gliricidia leaves 70% + 30% silage vegetable waste), and R2 (Gliricidia leaves 40% + 60% silage vegetable waste). Data were analyzed using analysis of variance (ANOVA) and further testing using the test Honestly Significant Difference (HSD). The results of ANOVA showed that the mixture of vegetable waste silage was highly significant (p <0.05) on dry matter digestibility and significantly (p <0.05) on the digestibility of organic matter. It can be concluded that the mixed of vegetable waste silage and Gliricidia leaves can improved digestibility of dry matter and organic matter, treatment of 40% and 60% Gliricidia leaves plus waste vegetable produce silage dry matter digestibility and percentage of organic matter is best (72,24% and 68,19%).Keyword: Silage vegetable waste, gliricidia leaves, dry and organic matter digestibilityABSTRAKPenelitian ini bertujuan untuk mengetahui kecenaan bahan kering dan bahan organik tingkat campuran silase sampah sayur dan daun gamal yang diuji secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 3 perlakuan dan 3 ulangan. Masing-masing perlakuan tersebut ialah R0 (daun gamal 100%), R1 (daun gamal 70% + silase sampah sayur 30%), dan R2 (daun gamal 40% + silase sampah sayur 60%). Data yang diperoleh dianalisis dengan menggunakan analisis sidik ragam (ANOVA) dan uji lanjut menggunakan uji Beda Nyata Jujur (BNJ). Hasil analisis sidik ragam menunjukkan bahwa campuran silase sampah sayur berpengaruh sangat nyata (p<0,05) terhadap kecernaan bahan kering dan berpengaruh nyata (p<0,05) terhadap kecernaan bahan organik. Dapat disimpulkan bahwa perlakuan campuran silase sampah sayur dan daun gamal dapat meningkatkan kecernaan bahan kering dan bahan organik, perlakuan 40% daun gamal dan 60% silase sampah sayur menghasilkan persentase kecernaan bahan kering dan bahan organik yang terbaik yaitu (72,24% dan 68,19%).Kata kunci : Silase sampah sayur, daun gamal, kecernaan bahan kering, dan bahan organik.

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Penggunaan Kulit Nanas Fermentasi dalam Ransum yang Mengandung Gulma Berkhasiat Obat Terhadap Konsumsi Nutrient Ayam Broiler

Jurnal Agripet ◽

10.17969/agripet.v16i2.4142 ◽

2016 ◽

Vol 16 (2) ◽

pp. 76

Author(s):

Wasir Ibrahim ◽

Rita Mutia ◽

Nurhayati Nurhayati ◽

Nelwida Nelwida ◽

Berliana Berliana

Keyword(s):

Nutrient Intake ◽

Broiler Chicken ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Pineapple Peel ◽

Nutrient Consumption ◽

Before And After ◽

Completely Randomized Design ◽

And Performance

ABSTRAK. Penelitian ini bertujuan untuk mengetahui penggunaan kulit nanas fermentasi ke dalam ransum yang mengandung gulma berkhasiat obat terhadap konsumsi nutrient ayam broiler. Penelitian ini menggunakan DOC jantan strain cob umur 2 hari sebanyak 200 ekor yang dipelihara selama 42 hari. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 5 ulangan. P0 = 0% tepung kulit nanas fermentasi (TKNF) dalam ransum mengandung 0% gulma obat(GO), (kontrol positif), P1 = 0% TKNF dalam ransum mengandung 2 % gulma obat (GO),(kontrol negatif), P2 = 7.5% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat, P3 = 15% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat, P4 = 22.5% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat. Parameter yang diamati kandungan gizi kulit nanas sebelum dan sesudah fermentasi dan konsumsi nutrient. Pengaruh yang nyata terhadap parameter yang diamati dilanjutkan dengan Uji Jarak Berganda Duncan, Data yang diperoleh dari setiap parameter dianalisis ragam (ANOVA) menggunakan bantuan software SPSS 16.0. Dari hasil penelitian disimpulkan bahwa penggunaan kulit nanas yang fermentasi dengan yoghurt dalam ransum yang mengandung gulma berkhasiat obat pada taraf 15% memberikan hasil terbaik pada konsumsi nutrient ayam broiler. (Fermented pineapple peel supplementation with addition of medicinal weeds on nutrient intake consumption of broiler chicken) ABSTRACT. The aim of this study was to determine the effect of using fermented pineapple peel in the ration containing medicinal weeds on nutrient consumption and performance of broiler . Two hundred 2 days male broiler chicken cob strain were used in this study and kept for 42 days. Completely randomized design (CRD)was applied with 5 treatments and 5 replicates. Treatments consisted of P0 = 0% of fermented pineapple peels meal (FPPM) in ration containing 0% medicinal weed (MW), positive control). P1 = 0% of fermented pineapple peels meal (FPPM) in the ration containing 2 % medicinal weed (MW), negative control). P2 = 7.5% of FPPM in the ration containing 2 % medicinal weed (MW). P3 = 15% of FPPM in the ration containing 2 % medicinal weed (MW). P4 = 22,5% of FPPM in the ration containing 2 % medicinal weed (MW). The parameters measured was nutrien intake before and after fermented peel and consumption. The observed data were analyzed by statistical product and service solution (SPSS 16.0). Results showed that use of fermented pineapple peel meal up to 15% with addition medicinal weeds was the best on nutrient consumption of broiler chicken.

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Evaluating Dentin Surface Treatments for Resin-Modified Glass Ionomer Restorative Materials

Operative Dentistry ◽

10.2341/12-162-l ◽

2013 ◽

Vol 38 (4) ◽

pp. 429-438 ◽

Cited By ~ 16

Author(s):

TA Imbery ◽

A Namboodiri ◽

A Duncan ◽

R Amos ◽

AM Best ◽

...

Keyword(s):

Testing Machine ◽

In Vitro Study ◽

Positive Control ◽

Negative Control ◽

Surface Treatments ◽

Band Matrices ◽

Dentin Surface ◽

Occlusal Surfaces ◽

Significant Difference

SUMMARY This in vitro study evaluated the effect of six surface treatments on the shear bond strength of three resin-modified glass ionomers (RMGIs) to dentin. Occlusal surfaces of caries-free third molars were reduced to expose only dentin. Surface treatments were smear layer intact (negative control), Cavity Conditioner, EDTA, Ketac Primer, Self Conditioner, and etching with 35% phosphoric acid followed by the application of Optibond Solo Plus. Filtek Z250 composite resin bonded with Optibond Solo Plus served as a positive control. Conditioning agents were used according to the manufacturers' instructions. After surface treatments, Fuji II LC, Riva LC, Ketac Nano, and Filtek Z250 were placed in copper-band matrices 5 mm in diameter and 2 mm in height and were light-cured for 20 seconds. Specimens were stored in 100% humidity for 24 hours, after which they were placed in deionized water for 24 hours at 37°C. They were then tested under shear forces in an Instron Universal Testing Machine at a crosshead speed of 0.5 mm/min. A two-way analysis of variance and Tukey honestly significant difference statistical analyses (p<0.05) indicated significant interaction between RMGIs and conditioning agents. Acid etching followed by Optibond Solo Plus provided highest bond strengths for all three RMGIs, which were not statistically different from the positive control.

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