scholarly journals Production and Purification of Cutinase from <i>Fusarium oxysporum</i> Using Modified Growth Media and a Specific Cutinase Substrate

2015 ◽  
Vol 06 (04) ◽  
pp. 245-258 ◽  
Author(s):  
Ofir Degani
Keyword(s):  
Fusarium Oxysporum ◽  
Growth Media

scholarly journals Potential antifungal effects of silver nanoparticles (AgNPs) of different sizes against phytopathogenic Fusarium oxysporum f. sp. radicis-lycopersici (FORL) strains

2021 ◽  
Vol 3 (4) ◽  
Author(s):  
Ilgin Akpinar ◽  
Muammer Unal ◽  
Taner Sar
Keyword(s):  
Silver Nanoparticles ◽  
Fusarium Oxysporum ◽  
Antifungal Agents ◽  
New Technology ◽  
Economic Loss ◽  
Phytopathogenic Fungi ◽  
Growth Media ◽  
Mycelium Growth ◽  
Antifungal Effects ◽  
Critical Problems

AbstractFusarium species are the primary fungal pathogen affecting agricultural foodstuffs both in crop yield and economic loss. Due to these problems, control of phytopathogenic fungi has become one of the critical problems around the World. Nanotechnology is a new technology with potential in many fields, including agriculture. This study focused on determining potential effects of silver nanoparticles (AgNPs) with different nanosizes (3, 5, 8 and 10 nm) and at different concentrations (12.5–100 ppm) against phytopathogenic Fusarium oxysporum f. sp. radicis-lycopersici (FORL) strains. The maximum antifungal activity was achieved by decreasing nanosize and increasing concentration of AgNPs. Mycelium growth abilities were decreased about 50%, 75% and 90% by AgNPs treatment with 3 nm sizes at 25 ppm, 37.5 ppm and 50 ppm concentrations, respectively. The productivity of fungal biomass in the liquid growth media was found to be too limited at the 25–37.5 ppm of AgNPs concentrations with all sizes. In addition, both septation number and dimensions of micro- and macroconidia were found to be gradually decreased with the application of silver nanoparticles. This work showed that the low concentration of AgNPs could be used as potential antifungal agents and applied for control of phytopathogens.

scholarly journals CHARACTERIZATION OF Fusarium oxysporum ISOLATES AND RESISTANCE OF PASSION FRUIT GENOTYPES TO FUSARIOSIS

2017 ◽  
Vol 39 (3) ◽  
Author(s):  
LETÍCIA MAGALHÃES TEIXEIRA ◽  
LÍSIAS COELHO ◽  
NILVANIRA DONIZETE TEBALDI
Keyword(s):  
Fusarium Oxysporum ◽  
Mycelial Growth ◽  
Block Design ◽  
Field Resistance ◽  
Passion Fruit ◽  
Malt Extract Agar ◽  
Growth Media ◽  
Malt Extract ◽  
Extract Agar

ABSTRACT Passion fruit wilt, caused by Fusarium spp., is one of the most severe diseases for this crop. Although yellow passion fruit is propagated mostly by seeds, the use of rootstocks tolerant to early death could be a management strategy for cultivation in areas with disease history. Thus, this study characterized Fusarium isolates obtained in Triângulo Mineiro and determined the most suitable genotype to use for grafting to reduce losses caused by this pathogen. Mycelial growth and sporulation of Fusarium isolates, in three different growth media, was quantified by measuring colony diameter and by counting the conidia in Neubauer chamber 10 days after growth in malt extract-agar 2%, PDA or CMA at 22 ± 3ºC and 12 hours lighting. Five days later the color of the colonies was evaluated. The experimental design was a 4x3 factorial completely randomized design, with five replications. Characterization of morphological structures of isolates was done with minimum cultivation in Malt extract-agar 2% amended with sterilized soil + sand (1:1). Three Passiflora species and two seedling types were used as rootstock to evaluate field resistance to fusariosis, in a randomized block design, as a 3x2 factorial, with three Passiflora species (P. alata, P. setacea and P. edulis) and two seedling types (ungrafted or grafted with P. edulis). The medium PDA was the least favorable for mycelial growth of the isolates. The best medium for conidium production was Malt extract and the most sporulating isolates were Fus-01 and Fus-02. Isolate color varied from white to pink to violet. Only Fus-02 and Fus-04 formed macro and microconidia. Shape, dimensions and septa number of macroconidia and microconidia, as well as the presence of characteristic monophyalides allowed the classification of all isolates as Fusarium oxysporum f.sp. passiflorae. Passiflora alata and P. setacea, used as rootstocks for P. edulis in the field, were resistant to fusariosis. Despite its low survival, P. edulis grafted on itsef, promoted similar growth of secondary branches as P. setacea in the field.

scholarly journals Evaluation of the Antifungal Activity of Gold–Chitosan and Carbon Nanoparticles on Fusarium oxysporum

Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1143 ◽  
Author(s):  
Florin-Daniel Lipșa ◽  
Elena-Laura Ursu ◽  
Cristian Ursu ◽  
Eugen Ulea ◽  
Ana Cazacu
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Carbon Nanoparticles ◽  
Fungal Growth ◽  
Colony Growth ◽  
Plant Diseases ◽  
Economic Losses ◽  
Growth Media ◽  
Nanoparticle Concentration ◽  
Tomato Production

Nanoparticles are implemented in different biotechnological fields, and there is interest in their use in plant biology. Nanotechnology can help overcome the persistent limitations of using conventional fungicides in the management of plant diseases, contributing to a safer environment. Hence, this study is focused on evaluating the behavior of nanoparticles on two different strains of Fusarium oxysporum, which have a wide-ranging occurrence in tomato production and account for important economic losses. Fusarium oxysporum is an ascomycetous fungus that is well-known as a soilborne plant pathogen, adapted to any soil type, and it lives in different forms on organic materials. Gold–chitosan and carbon nanoparticles were suspended in potato dextrose agar growth media, and their antifungal activity was evaluated at 1, 3, 5, and 7 days after incubation by measuring the diameter of fungal colonies. The results showed that the nanoparticles have antifungal properties against F. oxysporum, the fungal colony growth diameter being reduced. Likewise, it was observed that the colony diameter was smaller when the nanoparticle concentration increased. However, in the case of one F. oxysporum strain, the highest nanoparticle concentration applied during the experiment’s execution was not able to completely inhibit fungal growth.

SINTESIS DAN MODIFIKASI STRUKTUR DAN PORI MEMBRAN KITOSAN TERCROSSLINK UNTUK STERILISASI MEDIA PERTUMBUHAN BAKTERI

2019 ◽  
Vol 3 (2) ◽  
pp. 27
Author(s):  
Emma Savitri ◽  
Natalia Suseno ◽  
Tokok Adiarto
Keyword(s):  
Tensile Strength ◽  
Chitosan Solution ◽  
Curing Temperature ◽  
Growth Media ◽  
Optimum Conditions ◽  
Separation Processes ◽  
Crosslinking Process ◽  
Chitosan Membrane ◽  
Chitosan Membranes ◽  
Crosslinked Chitosan

Many mass-transfer applications have used chitosan membrane in separation processes. This research applied crosslinked chitosan membrane to sterillize bacterial growth media. Chitosan membranes having 79 % DD were produced by casting and drying chitosan solution. The images of the membrane were characterized by SEM and other characterizations such as permeability, permselectivity and tensile strength were investigated. The flux increased with longer submersion period but the rejection decreased. Otherwise, the flux decreased and rejection increased in line with an increase in curing temperature. Tensile strength increased with the increase of submersion period and curing temperature. The optimum conditions of crosslinking process are 2 hours of submersion periods and curing temperature at 90 oC.  It gives flux 5.8930 L/jam.m2, rejection 97.47 % and tensile strength 49640 kN/m2

Evaluation of Pine Bark, Pine Bark With Wood, and Pine Tree Chips as Components of a Container Plant Growing Media

1986 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
A.J. Laiche ◽  
V.E. Nash
Keyword(s):  
Organic Amendments ◽  
Pine Bark ◽  
Growth Media ◽  
Ligustrum Sinense ◽  
Pine Tree ◽  
Water Holding ◽  
Low Solubility ◽  
Low Nitrogen ◽  
Very High ◽  
Container Plant

Three woody landscape species, Rhododendron indica ‘President Clay’, Ligustrum sinense ‘variegata’, and Ilex crenata ‘compacta’, were grown in media prepared from fresh pine bark, pine bark with wood, and pine tree chips. Although media were variable in physical properties, all exhibited very high hydraulic conductivity and low water holding capacity. The capacity of these media materials to hold fertilizer elements was very low. Nitrogen, potassium, and phosphorus were rapidly removed by leaching while calciuum and magnesium were retained longer because of the low solubility of dolomitic limestone. Pine bark was the best growth media tested for all plant species. Pine bark with wood was less satisfactory than pine bark and growth was poorest in pine tree chips. More research is needed on the use of the organic amendments with greater amounts of wood before being widely used as organic components of growth media.

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